Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,018,253 C→T 5.6% A270A (GCG→GCA proC ← pyrroline‑5‑carboxylate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,018,2530CT 5.6% 321.5 / 6.5 107A270A (GCG→GCAproCpyrroline‑5‑carboxylate reductase
Reads supporting (aligned to +/- strand):  ref base C (48/51);  new base T (3/3);  total (53/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 110 total aligned reads displayed.

GTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGCAC  >  NZ_CP034943/2018114‑2018389
                                                                                                                                           |                                                                                                                                        
gTTAATGAACTGCTTCCAATCTTCACAACGAGTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATc                                                                                                                                          >  2:24935/1‑140 (MQ=255)
gTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATc                                                                                                                                          >  2:25054/1‑140 (MQ=255)
gTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATc                                                                                                                                          >  2:1391991/1‑140 (MQ=255)
 ttAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTAGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCg                                                                                                                                         >  2:1810450/1‑140 (MQ=255)
   aaTGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCt                                                                                                                                       <  2:1229277/140‑1 (MQ=255)
      gAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCttta                                                                                                                                    >  1:1397909/1‑138 (MQ=255)
      gAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTga                                                                                                                                    >  1:96681/1‑140 (MQ=255)
           gCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAAt                                                                                                                               >  1:2149008/1‑140 (MQ=255)
           gCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAAt                                                                                                                               >  1:986088/1‑140 (MQ=255)
           gCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCCTCGCTGATTTCCTTTTAAAGCTTAGCCGCATGATTAATCCCTTGAGAAAt                                                                                                                               >  1:305429/1‑140 (MQ=255)
           gCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTAGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTGGAGaact                                                                                                                               >  2:2276485/1‑138 (MQ=255)
            cTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATc                                                                                                                              >  1:1770612/1‑140 (MQ=255)
            cTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATc                                                                                                                              >  1:1770791/1‑140 (MQ=255)
            cTTCCAATCTTCACAACGACTCTTTTCATGCTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGCGCTGCAGTTTTTTCGATATCTTAGCTGATTTCCATTGAACGCATAGCCGCATGCTTTATTGCTTGAGAAATc                                                                                                                              >  1:1770702/1‑140 (MQ=255)
                 aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAACTCGCTtc                                                                                                                         >  1:1397227/1‑140 (MQ=255)
                 aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  1:2379232/1‑140 (MQ=255)
                 aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  1:570579/1‑140 (MQ=255)
                 aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  1:1475138/1‑140 (MQ=255)
                 aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  2:692276/1‑140 (MQ=255)
                 aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  2:738649/1‑140 (MQ=255)
                 aaTCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCGTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTtc                                                                                                                         >  1:825746/1‑140 (MQ=255)
                      tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTCTCACTCCTGATAGCGGTGCCGCGGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGGGAAATCGCTTCTccgc                                                                                                                    >  1:58867/1‑140 (MQ=255)
                      tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTGGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTacgc                                                                                                                    >  2:1638923/1‑140 (MQ=255)
                      tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGTACGCTTAGCCGCATGCTTAATCGCTTTAGAAATCGCTTCTccgc                                                                                                                    >  1:1415146/1‑140 (MQ=255)
                      tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGTACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTacgc                                                                                                                    >  1:2264636/1‑140 (MQ=255)
                      tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTccgc                                                                                                                    >  2:2296008/1‑140 (MQ=255)
                      tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTccgc                                                                                                                    >  2:837090/1‑140 (MQ=255)
                      tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTccgc                                                                                                                    >  1:758545/1‑140 (MQ=255)
                      tCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTccgc                                                                                                                    >  2:2293762/1‑140 (MQ=255)
                             gACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGct                                                                                                             <  1:45569/140‑1 (MQ=255)
                               ctctTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGctct                                                                                                           >  1:1493839/1‑140 (MQ=255)
                                    ttCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTc                                                                                                      >  1:2248772/1‑140 (MQ=255)
                                    ttCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTc                                                                                                      <  1:1995833/140‑1 (MQ=255)
                                            tGTATCAGGCGTCACTTTCTCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGGCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTg                                                                                              <  2:1147786/140‑1 (MQ=255)
                                            tGTATCAGGCGTCACTTTATCACTCCTGATAGCCGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTACTTTGAACGCTTATCCGCAATCTTAATAGCTTTAGAAAAAGATTCAACGCCGCCGCTCATTTTAAATGCTTg                                                                                              >  1:952670/1‑140 (MQ=255)
                                            ggTATCAGGCGTCACTGTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGAGATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCGTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTg                                                                                              <  2:2214604/139‑1 (MQ=255)
                                               aTCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAg                                                                                           <  2:1416370/140‑1 (MQ=255)
                                               aTCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAg                                                                                           <  2:2204814/140‑1 (MQ=255)
                                               aTCAGGCGTCACTTTATCACTCCTGATAGCGGGGCCGCGGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAg                                                                                           <  2:2317540/140‑1 (MQ=255)
                                                 cAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGcc                                                                                         <  1:947941/140‑1 (MQ=255)
                                                 cAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGcc                                                                                         <  1:2325655/140‑1 (MQ=255)
                                                   ggCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCg                                                                                       >  2:413288/1‑140 (MQ=255)
                                                        cACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCGTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGgcagc                                                                                  >  1:1686859/1‑140 (MQ=255)
                                                         aCTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCTATTCTTGAAGCCCGgcagca                                                                                 <  2:1211726/140‑1 (MQ=255)
                                                         aCTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGATCTTTTTCTATTATTGCAGCCCGgcagca                                                                                 <  2:2139808/140‑1 (MQ=255)
                                                          cTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGAGTTCCTTTGAACGCTTAGCCGCATGCGTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGcagcag                                                                                <  2:1919760/140‑1 (MQ=255)
                                                              aTCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgt                                                                            >  2:312078/1‑140 (MQ=255)
                                                              aTCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgt                                                                            >  2:1900003/1‑140 (MQ=255)
                                                                     cTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgttccgttc                                                                     >  2:2037370/1‑140 (MQ=255)
                                                                       gATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCCTGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCgg                                                                   <  2:76660/140‑1 (MQ=255)
                                                                       gATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCgg                                                                   <  1:1411996/140‑1 (MQ=255)
                                                                        aTAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTTAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTACGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGGTCggg                                                                  >  2:1173041/1‑139 (MQ=255)
                                                                        aTAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGt                                                                  >  2:1961232/1‑140 (MQ=255)
                                                                         tAGCGGTTCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTg                                                                 <  2:286166/140‑1 (MQ=255)
                                                                         tAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTg                                                                 <  2:1312163/140‑1 (MQ=255)
                                                                          aGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGa                                                                >  2:134779/1‑140 (MQ=255)
                                                                             ggTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGt                                                             >  2:683191/1‑140 (MQ=255)
                                                                               tGCCGCTGTTTTTTCGATATCGTCGCTGATTTGCTTTTAACGCTTAGCCGCAAGCTTAATCCCTTGAGAAATAGCGTCTCCGCCGCCGCTCTTTTTAAAGGCGTGGAGCCCGGCAGCAGTCGTTACGTTCGGTGACGTGa                                                           >  1:1378907/1‑140 (MQ=255)
                                                                               tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGTAAGCTTAGCCCCATGCTTAATAGCTTGAGAAATCGCTTCTCCCCCGCCGCTCTTTTTCAAAGCATTCAGCCCGGCAGCATTCGTTCCGTTCGGTGACGTGa                                                           >  1:1312163/1‑140 (MQ=255)
                                                                               tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAACTCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGa                                                           >  1:1774092/1‑140 (MQ=255)
                                                                               tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTg                                                                 <  2:1735980/134‑1 (MQ=255)
                                                                               tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTg                                                                 >  1:1735980/1‑134 (MQ=255)
                                                                               tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGa                                                           >  2:256436/1‑140 (MQ=255)
                                                                               tGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGa                                                           >  2:2118846/1‑140 (MQ=255)
                                                                                    cTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTg                                                      <  1:256436/140‑1 (MQ=255)
                                                                                    cTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTg                                                      <  2:736121/140‑1 (MQ=255)
                                                                                    cTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTg                                                      <  1:1349954/140‑1 (MQ=255)
                                                                                     tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATAgtgt                                                     <  2:621389/140‑1 (MQ=255)
                                                                                     tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGt                                                     <  2:1586947/140‑1 (MQ=255)
                                                                                     tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGt                                                     <  2:1134530/140‑1 (MQ=255)
                                                                                     tGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTGAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGt                                                     <  2:376127/140‑1 (MQ=255)
                                                                                          tttCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCtt                                                <  2:1770791/140‑1 (MQ=255)
                                                                                          tttCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCtt                                                <  2:1770702/140‑1 (MQ=255)
                                                                                          tttCGATATCTTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTTATTGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCCGCTGCTGTCGTTCCGTTCGGTGACGTGATATTATCTCtt                                                <  2:1770612/140‑1 (MQ=255)
                                                                                           ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  2:679626/140‑1 (MQ=255)
                                                                                           ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  1:60414/140‑1 (MQ=255)
                                                                                           ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  1:1764634/140‑1 (MQ=255)
                                                                                           ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  2:2345764/140‑1 (MQ=255)
                                                                                           ttCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  1:2134598/140‑1 (MQ=255)
                                                                                               atatCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgtt                                                                           <  2:748094/108‑1 (MQ=255)
                                                                                               atatCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTcgtt                                                                           >  1:748094/1‑108 (MQ=255)
                                                                                                tatCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGAcc                                          <  1:2037370/140‑1 (MQ=255)
                                                                                                  tcgtcgCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGACATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTc                                        <  2:2248772/140‑1 (MQ=255)
                                                                                                     tcgCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAgg                                     <  1:2293762/140‑1 (MQ=255)
                                                                                                        cTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTg                                  >  2:2132982/1‑140 (MQ=255)
                                                                                                             ttCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTtctc                             <  2:348427/140‑1 (MQ=255)
                                                                                                                          ttAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTa                                               <  1:2118143/109‑1 (MQ=255)
                                                                                                                                    gCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  1:2132982/140‑1 (MQ=255)
                                                                                                                                    gCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  2:2358275/140‑1 (MQ=255)
                                                                                                                                    gCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  1:2118846/140‑1 (MQ=255)
                                                                                                                                    gCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  1:1909182/140‑1 (MQ=255)
                                                                                                                                    gCTTAATCGCGTGAGAAATCGCTGCTCCGCCGCCGCTCTTTGTCCAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCg      <  2:600510/140‑1 (MQ=255)
                                                                                                                                     cTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCgg     <  1:312078/140‑1 (MQ=255)
                                                                                                                                     cTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCgg     <  1:1138409/140‑1 (MQ=255)
                                                                                                                                     cTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCgg     <  1:1900003/140‑1 (MQ=255)
                                                                                                                                      ttAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGc    <  1:1814223/140‑1 (MQ=255)
                                                                                                                                      ctAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGc    <  1:837090/139‑1 (MQ=255)
                                                                                                                                        aaTCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGCAc  >  1:543879/1‑140 (MQ=255)
                                                                                                                                        aaTCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTAAATGAGCATTTTTGCGGCAc  >  2:118611/1‑140 (MQ=255)
                                                                                                                                        aaTCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCGCAGGCGGTTCTCCTGTTTCCATGAGCCTTTTTGCGGCAc  >  1:987792/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GTTAATGAACTGCTTCCAATCTTCACAACGACTCTTTTCATGTTTGTATCAGGCGTCACTTTATCACTCCTGATAGCGGTGCCGCTGTTTTTTCGATATCGTCGCTGATTTCCTTTGAACGCTTAGCCGCATGCTTAATCGCTTGAGAAATCGCTTCTCCGCCGCCGCTCTTTTTCAAGGCTTGCAGCCCGGCAGCAGTCGTTCCGTTCGGTGACGTGATATTGTCTCTTAAGACCTCAGGCTGTTCTCCTGTTTCCATGAGCATTTTTGCGGCAC  >  NZ_CP034943/2018114‑2018389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: