Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,117,175 G→T 5.1% R535R (CGG→AGG)  EO946_RS10610 ← nitric oxide reductase activation protein NorD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,117,1750GT 5.1% 247.3 / 3.2 79R535R (CGG→AGG) EO946_RS10610nitric oxide reductase activation protein NorD
Reads supporting (aligned to +/- strand):  ref base G (29/46);  new base T (2/2);  total (31/48)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

TCTGGCTTCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCAT  >  NZ_CP034943/2117038‑2117306
                                                                                                                                         |                                                                                                                                   
tCTGGCTTCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGa                                                                                                                                   <  2:205672/140‑1 (MQ=255)
       tCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATa                                                                                                                            <  2:8825/140‑1 (MQ=255)
       tCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATa                                                                                                                            <  2:289954/140‑1 (MQ=255)
            aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                       <  2:1875635/140‑1 (MQ=255)
            aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                       <  2:1758128/140‑1 (MQ=255)
            aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                       <  2:1082038/140‑1 (MQ=255)
              ccGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                     <  1:1283397/140‑1 (MQ=255)
               cGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCAc                                                                                                                    <  1:1742157/140‑1 (MQ=255)
                 cTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCACCATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACgg                                                                                                                  <  2:1280223/140‑1 (MQ=255)
                 cTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACgg                                                                                                                  <  1:2022080/140‑1 (MQ=255)
                   tCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGtt                                                                                                                >  1:727950/1‑140 (MQ=255)
                   tCACTTGTATCGACAAACCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGtt                                                                                                                >  2:1690574/1‑140 (MQ=255)
                     aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                              <  2:2146185/140‑1 (MQ=255)
                     aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                              <  1:638022/140‑1 (MQ=255)
                     aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                              >  2:40514/1‑140 (MQ=255)
                      cTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATc                                                                                                             <  2:2307263/140‑1 (MQ=255)
                      agtggaTCGACAATCCCGTTTTCTTCAAAGCCAAACGGGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACATTGGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCCTCTGCCGGATCGCATAGCCGTCACGGTTATc                                                                                                             <  2:641033/135‑1 (MQ=255)
                         gTATCGACAATCCCGTTTTGTTCATAGCCAAAAGCGGCCGGCTCTCCATCTGAAAAAACGATCAATAACTTTTGCGCCTCGCTGAGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTc                                                                                                          >  2:1755670/1‑140 (MQ=255)
                           agcGACAATCCCGGTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGGTGCAACATGTTTTTCGTCATCTGCCGGCTCGCATCGCCGTCCCGGTAATCTTccg                                                                                                        >  2:528556/3‑139 (MQ=255)
                           aTCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCt                                                                                                        <  2:1108780/140‑1 (MQ=255)
                            tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                     <  2:2381024/126‑1 (MQ=255)
                            tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                     >  1:2381024/1‑126 (MQ=255)
                            tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                       >  1:2033516/1‑140 (MQ=255)
                            tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                       >  1:2288289/1‑140 (MQ=255)
                            tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                       >  1:1777856/1‑140 (MQ=255)
                               acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                             >  2:45862/4‑131 (MQ=255)
                               acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                             <  1:45862/128‑1 (MQ=255)
                               acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                             >  2:1707414/4‑131 (MQ=255)
                               acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAAGTGCGTACCCGTCACGGTTATc                                                                                                             <  1:1707414/128‑1 (MQ=255)
                                   tCCCGTTTTGTTCATATCCAAACGCGGCAGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCAGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                >  2:2378942/1‑140 (MQ=255)
                                   tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                >  1:1977719/1‑140 (MQ=255)
                                   tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                <  2:1452790/140‑1 (MQ=255)
                                   tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                >  2:2007602/1‑140 (MQ=255)
                                   tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                >  2:940590/1‑140 (MQ=255)
                                   tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTAAGGCTCa                                                                                                >  2:75090/1‑140 (MQ=255)
                                   tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCAAAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                >  2:408201/1‑140 (MQ=255)
                                   tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCAGCAACATTTTATTCGTCATCTGACGGATCGCATACCCGTCACGGTTATCTTCCTCAGtctcc                                                                                                >  1:18138/1‑139 (MQ=255)
                                    cccGTGTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCACCATTTTTTTCGTCATCTGACGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCaa                                                                                               >  1:1160626/1‑140 (MQ=255)
                                     cctttttGTTCATAGCCAAACGCGGCCGTCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                              <  1:2110496/137‑1 (MQ=255)
                                     ccGTTTTTTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                              <  1:1026631/140‑1 (MQ=255)
                                     ccGTTTTGTTCATAGCCAAACGCTGCCGGGTCTACATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                              <  1:1390657/140‑1 (MQ=255)
                                     ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                              <  2:1977719/140‑1 (MQ=255)
                                     ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                              <  2:988396/140‑1 (MQ=255)
                                     ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                              <  1:2066292/140‑1 (MQ=255)
                                          ttGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGTCTCAAGCTGCa                                                                                         >  2:2022858/1‑140 (MQ=255)
                                             ttCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATaa                                                                                      >  2:1412855/1‑140 (MQ=255)
                                                  aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                 >  2:1252849/1‑140 (MQ=255)
                                                  aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                 >  1:229607/1‑140 (MQ=255)
                                                  aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                 >  2:1859660/1‑140 (MQ=255)
                                                       aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                            <  1:2035123/140‑1 (MQ=255)
                                                       aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                            <  1:1499372/140‑1 (MQ=255)
                                                       aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                            <  1:2076022/140‑1 (MQ=255)
                                                            ggccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCct                                                                       <  2:1123135/140‑1 (MQ=255)
                                                             gccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                      <  2:538170/140‑1 (MQ=255)
                                                              ccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctct                                                                     <  1:1690574/140‑1 (MQ=255)
                                                              ccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctct                                                                     >  1:1599346/1‑140 (MQ=255)
                                                                      ccATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTaa                                                             <  2:437198/140‑1 (MQ=255)
                                                                             aaaaaaCGATCAAGAACTTTTGCGCCTCGCTGCGCTTCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATgatgat                                                      <  2:703985/140‑1 (MQ=255)
                                                                             aaaaaaCGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATgatgat                                                      <  2:459298/140‑1 (MQ=255)
                                                                                       cAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGaa                                            <  2:229607/140‑1 (MQ=255)
                                                                                        aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                           <  2:2203861/140‑1 (MQ=255)
                                                                                        aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                           <  2:802680/140‑1 (MQ=255)
                                                                                        aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                           <  2:170950/140‑1 (MQ=255)
                                                                                           aaCTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAAc                                        <  1:905865/140‑1 (MQ=255)
                                                                                                    gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                               <  2:2307467/140‑1 (MQ=255)
                                                                                                    gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                               <  1:40514/140‑1 (MQ=255)
                                                                                                    gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTGATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                               <  2:1220001/140‑1 (MQ=255)
                                                                                                             cgctgcAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTtgg                      <  1:1755670/140‑1 (MQ=255)
                                                                                                                  cAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTtggctggc                 >  2:1298388/1‑140 (MQ=255)
                                                                                                                     cATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                      >  1:1456202/1‑84 (MQ=255)
                                                                                                                     cATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                      <  2:1456202/84‑1 (MQ=255)
                                                                                                                     cATTTTTTTCGTCATCTGCCGGATCGCATAGCCCTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCGCTTGTCTTAATGAGGCTTGAAAGGGAATACCCGTATTGAAATAGTTTGGCTGGCTTg              >  2:1658619/1‑140 (MQ=255)
                                                                                                                          ttttCGTCATCTGCCGGATCGCCTAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCg         >  2:1564703/1‑140 (MQ=255)
                                                                                                                          ttttCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGTCTGGCTTGTTTCg         >  2:2098966/1‑140 (MQ=255)
                                                                                                                            ttCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGCTGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGt       <  1:1175344/140‑1 (MQ=255)
                                                                                                                                tCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCa   <  1:1213706/140‑1 (MQ=255)
                                                                                                                                tCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGAGTGAAAGGGAATAACAGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCa   >  2:1149028/1‑140 (MQ=255)
                                                                                                                                 cATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCAt  <  2:1375246/140‑1 (MQ=255)
                                                                                                                                 cATCTGCCGGATCGCAGAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCAt  <  2:34980/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                   
TCTGGCTTCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCAT  >  NZ_CP034943/2117038‑2117306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑