BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	417,261	T→A	6.7%	L58L (CTA→CTT)	EO946_RS02145 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	417,261	0	T	A	6.7%	320.7 / 7.3	102	L58L (CTA→CTT)	EO946_RS02145	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (45/49); new base A (5/3); total (50/52)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

CATCGGCAATCGCATCTTCAAAGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAA  >  NZ_CP034943/417122‑417399

catcGGCAATCGCATCTTCAAAGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACt                                                                                                                                            <  2:1990022/140‑1 (MQ=255)

         tCGCATCTTCAAAGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCGTCAGGGGTTCTTTTTTTTCGATTTATAGATAAAGTACATTTCTATCACTAGTATGaaa                                                                                                                                   >  2:253245/1‑140 (MQ=255)

                    aaGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATaa                                                                                                                        <  2:605034/140‑1 (MQ=255)

                    aaGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATaa                                                                                                                        <  2:1133297/140‑1 (MQ=255)

                        aaCCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGTGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGc                                                                                                                    <  2:1348699/140‑1 (MQ=255)

                        aaCCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGc                                                                                                                    <  2:909001/140‑1 (MQ=255)

                        aaCCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGc                                                                                                                    <  2:418723/140‑1 (MQ=255)

                        aaCCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGc                                                                                                                    <  2:2250426/140‑1 (MQ=255)

                          ccATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGAGCACCCCATAACCGGTACTAATATTTAGAATTATAAAAAAAGTAAATTAATATAAAAAATATGAAAAAAAAAAATaaaacaaa                                                                                                                  >  2:1264130/1‑136 (MQ=255)

                          caaTCAACAGACATCCCAGCTATACCATAGACGGAAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCAAGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCAc                                                                                                                  <  2:746365/138‑1 (MQ=255)

                             tCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                               <  1:788335/140‑1 (MQ=255)

                             tCAACAGACATAGCAGCGATACAATAGACGGAAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                               <  1:640907/140‑1 (MQ=255)

                             tCAACAGACATAGCAGCGATACAATAGACGGAAATATTATCGATTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                               <  1:83589/140‑1 (MQ=255)

                                aCAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGTTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCa                                                                                                            <  2:650020/140‑1 (MQ=255)

                                aCAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCa                                                                                                            <  2:1647518/140‑1 (MQ=255)

                                 caaacacAGCAGTGATACAATAGACGGCAATATGATTGTCTGAAAAAAGCGCCCCCCTTTAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAAGCACGACCCAt                                                                                                           >  1:1782591/4‑140 (MQ=255)

                                 caaacacAGCAGTGATACAATAGACGGCAATATGATTGTCTGAAAAAAGCGCCCCCCTTTAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAAGCACGACCCAt                                                                                                           >  1:2132473/4‑140 (MQ=255)

                                    aCATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCt                                                                                                        >  1:1123439/1‑140 (MQ=255)

                                        agcagcGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCaa                                                                                                    >  1:1530778/1‑140 (MQ=255)

                                        agcagcGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCaa                                                                                                    >  1:1603559/1‑140 (MQ=255)

                                              gATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                               <  2:1198427/123‑1 (MQ=255)

                                              gATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                               >  1:1198427/1‑123 (MQ=255)

                                               aTACAATCTACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAAcgtcgt                                                                                             <  1:1197070/140‑1 (MQ=255)

                                               aTACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAACAATAAAAGCACGACCCCTGCTGCAAAcggcgt                                                                                             >  1:1728080/1‑140 (MQ=255)

                                               aTACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAAcgtcgt                                                                                             >  1:1652162/1‑140 (MQ=255)

                                               aTACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAAcgtcgt                                                                                             <  2:1469517/140‑1 (MQ=255)

                                               aTACAATAGACCGAAATATTATCGCTTGAAAAAAGCCCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAACCACGACCCATTCTGCAAAcgtcgt                                                                                             <  1:1365505/140‑1 (MQ=255)

                                                  cAATAGACGGCACTATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCAt                                                                                          >  1:1802236/1‑140 (MQ=255)

                                                    aTAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCt                                                                                        <  2:268888/140‑1 (MQ=255)

                                                    aTAGACGGCAATATTATCGATTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCt                                                                                        <  1:2142580/140‑1 (MQ=255)

                                                    aTAGACGGAAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCt                                                                                        <  1:315176/140‑1 (MQ=255)

                                                       gACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTATTATGAAAACATAAAATAAAATCACGACCCATTATGCAAACGTCGTCATCTTaa                                                                                     >  1:366454/1‑140 (MQ=255)

                                                          ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTCTAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCCTTCTGCAAACGTCGTCATCTTAAAGc                                                                                  >  1:1257629/1‑140 (MQ=255)

                                                          ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATATATAACGTACATTTCTATCAATAGTATTAAAACAAAAAATAAAAAAACGACACATTATTAAAAAGAAGTAATCTTAAAga                                                                                  >  2:2121351/1‑139 (MQ=255)

                                                          ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                  <  1:601902/140‑1 (MQ=255)

                                                          ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                  >  1:1133411/1‑140 (MQ=255)

                                                          ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                  >  1:526042/1‑140 (MQ=255)

                                                          ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                  >  1:1460797/1‑140 (MQ=255)

                                                          ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                  >  1:1189230/1‑140 (MQ=255)

                                                           gCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTCATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCCCGACCCATTCTGCAAACGTCGTCATCTTAAAGCt                                                                                 <  2:521620/140‑1 (MQ=255)

                                                            cAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCTATTTATATATAACGTAAATTTCTCTAAAAAGTATGAAAACAAAAAATAAAAGCAAGACCCATTCTGCAAAAGTAGTCATCTTAAAGCTg                                                                                >  2:601902/1‑140 (MQ=255)

                                                            cAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTg                                                                                >  1:666178/1‑140 (MQ=255)

                                                            cAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTAGATATATAGATAAAGTACATTTCTCTCAAAAGAATGAAAACAAAAAAAAAAAGAACGAAACAATATGAAAAAGTCGTAATCATAAAGATg                                                                                >  2:1005625/1‑140 (MQ=255)

                                                                  tatCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGCTCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAACGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACata                                                                          <  2:473562/140‑1 (MQ=255)

                                                                  tatCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACata                                                                          <  1:2120089/140‑1 (MQ=255)

                                                                  tatCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACata                                                                          <  2:2116544/140‑1 (MQ=255)

                                                                  tatCGATTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACata                                                                          <  1:1510337/140‑1 (MQ=255)

                                                                    tCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTATCTCACTAGTATGAAAACAAAAAATAAAAGCACGAACCATTATGCAAACTTAGTCATATTAAAGCTGGACataat                                                                        >  2:191365/1‑138 (MQ=255)

                                                                    tCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATAAAGAAAAATTCTCTCACTAGTATGAAAACAAAAAATAAAAGAACGACCCATTCTGAAAACATCGTAATCTTAAAGAAGGAAATAtt                                                                        >  2:1984781/1‑140 (MQ=255)

                                                                    tCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTATATTTATATATAAAGTACAATACAATCACTAATATGAAAACAAAAAATAAAATAAAGACCAATTCTGAAAAAGTCGTCATCTTAAAGCTGGACAAAtt                                                                        >  2:2379541/1‑140 (MQ=255)

                                                                         tGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCTATATATAGATCACGTAAATTTATATAACTAGTATTAAAACAAAAAATAAAAGAAAGACACATACTGCAAACGACTACATCTTAAAGATTAACataaagatat                                                                   >  2:2405662/1‑133 (MQ=255)

                                                                         tGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTtat                                                                   >  1:118062/1‑140 (MQ=255)

                                                                         tGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTATCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTtat                                                                   >  1:1658809/1‑140 (MQ=255)

                                                                                      cctcTTCAGGTGTTATTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATTAAAAAAAAAAATAAAAGCACTACAAATTCTTCAAACGTAGTCATATTAAAGATGGAAATATTGTTATAATTGGCtttttt                                                      <  2:724008/137‑3 (MQ=255)

                                                                                      ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                      <  2:448942/140‑1 (MQ=255)

                                                                                      ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                      <  1:1731053/140‑1 (MQ=255)

                                                                                      ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                      <  1:910148/140‑1 (MQ=255)

                                                                                      ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                      <  1:149506/140‑1 (MQ=255)

                                                                                      ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                      <  1:1374539/140‑1 (MQ=255)

                                                                                      ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                      <  2:2363632/140‑1 (MQ=255)

                                                                                      ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGATGGACATATTGTTATAAATGGCTTTTGt                                                      <  2:591954/140‑1 (MQ=255)

                                                                                      ccGCTTCAGGGGTTCTATTTTTTCGATTTATAGATCACGTACCTTTCTCTCACTAGTATGAAAACAAAAAATAAAACCACGACCCAGTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                      <  2:1552617/140‑1 (MQ=255)

                                                                                        gCTTCAGGGTTTATTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCa                                                    <  1:68048/140‑1 (MQ=255)

                                                                                        gCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCa                                                    <  1:2039495/140‑1 (MQ=255)

                                                                                          ttCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                  >  2:701188/1‑108 (MQ=255)

                                                                                          ttCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                  <  1:701188/108‑1 (MQ=255)

                                                                                              ggggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGa                                              <  1:1321627/140‑1 (MQ=255)

                                                                                              ggggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGa                                              <  1:1910492/140‑1 (MQ=255)

                                                                                              ggggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGa                                              <  1:1996001/140‑1 (MQ=255)

                                                                                              ggggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGa                                              <  1:318984/140‑1 (MQ=255)

                                                                                              ggggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCAGTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGa                                              <  1:345847/140‑1 (MQ=255)

                                                                                               gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGaa                                             <  2:1825645/140‑1 (MQ=255)

                                                                                               gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGaa                                             <  2:1034466/140‑1 (MQ=255)

                                                                                               gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGaa                                             <  2:2091230/140‑1 (MQ=255)

                                                                                               gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGaa                                             <  2:2312965/140‑1 (MQ=255)

                                                                                               gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTCTCAAACTGaa                                             <  1:1162163/140‑1 (MQ=255)

                                                                                                   tCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAACACAAAATATAAAAGCACGACCCATTCTGCAAACGTCGTCCTCTTAAAGCTGGAGATATTGTTATAAATGGCTTGTGTCAAGCTGcaagcg                                         >  2:1311248/1‑137 (MQ=255)

                                                                                                   tCTTTATTTTCGATTTATAGATCACGTACATTTCTCTCCCTAGTATGAAAACAAAAAATAAAAGCACGACCCATACTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTCAAAccg                                         >  2:635206/1‑139 (MQ=255)

                                                                                                    cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                        >  2:235692/1‑140 (MQ=255)

                                                                                                    cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                        >  1:1886795/1‑140 (MQ=255)

                                                                                                    cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                        >  1:1766607/1‑140 (MQ=255)

                                                                                                    cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                        >  2:1465868/1‑140 (MQ=255)

                                                                                                    cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                        >  1:1631215/1‑140 (MQ=255)

                                                                                                    cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                        >  2:2316642/1‑140 (MQ=255)

                                                                                                    cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                        >  2:2314380/1‑140 (MQ=255)

                                                                                                               aTTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCAGTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTcc                             >  2:459487/1‑140 (MQ=255)

                                                                                                                 ttATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAATCACGACCCATTCCGCAAATGTTGTCATCTTAAAGCTGGACATATTGTTATAAACGGATTTTGTTAAACTGAACCCTTGAAGCATGTCCAt                           <  1:1237929/140‑1 (MQ=255)

                                                                                                                 ttATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAAGCACGACCCATTCCGCAAATGTTGTCATCTTAAAGCTGGACATATTGTTATAAACGGCTTTTGTTAAACTGAACCCTTGAAGCATGTCCAt                           <  2:2132473/140‑1 (MQ=255)

                                                                                                                 ttATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAAGCACGACCCATTCCGCAAATGTTGTCATCTTAAAGCTGGACATATTGTTATAAACGGCTTTTGTTAAACTGAACCCTTGAAGCATGTCCAt                           <  2:1782591/140‑1 (MQ=255)

                                                                                                                   ataGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATaa                         >  1:1479564/1‑140 (MQ=255)

                                                                                                                          aCGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGa                  <  2:118062/140‑1 (MQ=255)

                                                                                                                           cGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGaa                 <  2:224314/140‑1 (MQ=255)

                                                                                                                            gTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTCTAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATTTCCATAAAAACAGaaa                >  2:1216621/1‑140 (MQ=255)

                                                                                                                                      ctcACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTtaataa      >  2:2334282/1‑140 (MQ=255)

                                                                                                                                      ctcACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTtaataa      >  2:772791/1‑140 (MQ=255)

                                                                                                                                      ctcACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTtaataa      >  2:852309/1‑140 (MQ=255)

                                                                                                                                         aCTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACa   >  1:1699440/1‑140 (MQ=255)

                                                                                                                                         aCTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACa   >  1:81380/1‑140 (MQ=255)

                                                                                                                                         aCTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACa   >  1:416614/1‑140 (MQ=255)

                                                                                                                                          cTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACCTATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACaa  >  2:679831/1‑140 (MQ=255)

CATCGGCAATCGCATCTTCAAAGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAA  >  NZ_CP034943/417122‑417399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`