Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,117,182 A→G 5.1% Y532Y (TAT→TAC)  EO946_RS10610 ← nitric oxide reductase activation protein NorD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,117,1820AG 5.1% 252.6 / 2.9 79Y532Y (TAT→TAC) EO946_RS10610nitric oxide reductase activation protein NorD
Reads supporting (aligned to +/- strand):  ref base A (28/46);  new base G (2/2);  total (31/48)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

TCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCATCGTTTGTATC  >  NZ_CP034943/2117045‑2117316
                                                                                                                                         |                                                                                                                                      
tCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATa                                                                                                                                      <  2:8825/140‑1 (MQ=255)
tCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATa                                                                                                                                      <  2:289954/140‑1 (MQ=255)
     aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                                 <  2:1758128/140‑1 (MQ=255)
     aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                                 <  2:1082038/140‑1 (MQ=255)
     aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                                 <  2:1875635/140‑1 (MQ=255)
       ccGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                               <  1:1283397/140‑1 (MQ=255)
        cGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCAc                                                                                                                              <  1:1742157/140‑1 (MQ=255)
          cTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCACCATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACgg                                                                                                                            <  2:1280223/140‑1 (MQ=255)
          cTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACgg                                                                                                                            <  1:2022080/140‑1 (MQ=255)
            tCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGtt                                                                                                                          >  1:727950/1‑140 (MQ=255)
            tCACTTGTATCGACAAACCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGtt                                                                                                                          >  2:1690574/1‑140 (MQ=255)
              aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                                        <  2:2146185/140‑1 (MQ=255)
              aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                                        <  1:638022/140‑1 (MQ=255)
              aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                                        >  2:40514/1‑140 (MQ=255)
               cTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATc                                                                                                                       <  2:2307263/140‑1 (MQ=255)
               agtggaTCGACAATCCCGTTTTCTTCAAAGCCAAACGGGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACATTGGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCCTCTGCCGGATCGCATAGCCGTCACGGTTATc                                                                                                                       <  2:641033/135‑1 (MQ=255)
                  gTATCGACAATCCCGTTTTGTTCATAGCCAAAAGCGGCCGGCTCTCCATCTGAAAAAACGATCAATAACTTTTGCGCCTCGCTGAGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTc                                                                                                                    >  2:1755670/1‑140 (MQ=255)
                    agcGACAATCCCGGTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGGTGCAACATGTTTTTCGTCATCTGCCGGCTCGCATCGCCGTCCCGGTAATCTTccg                                                                                                                  >  2:528556/3‑139 (MQ=255)
                    aTCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCt                                                                                                                  <  2:1108780/140‑1 (MQ=255)
                     tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                               <  2:2381024/126‑1 (MQ=255)
                     tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                               >  1:2381024/1‑126 (MQ=255)
                     tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                                 >  1:1777856/1‑140 (MQ=255)
                     tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                                 >  1:2033516/1‑140 (MQ=255)
                     tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                                 >  1:2288289/1‑140 (MQ=255)
                        acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                                       >  2:45862/4‑131 (MQ=255)
                        acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                                       <  1:45862/128‑1 (MQ=255)
                        acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                                       >  2:1707414/4‑131 (MQ=255)
                        acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAAGTGCGTACCCGTCACGGTTATc                                                                                                                       <  1:1707414/128‑1 (MQ=255)
                            tCCCGTTTTGTTCATATCCAAACGCGGCAGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCAGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  2:2378942/1‑140 (MQ=255)
                            tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          <  2:1452790/140‑1 (MQ=255)
                            tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  2:2007602/1‑140 (MQ=255)
                            tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  1:1977719/1‑140 (MQ=255)
                            tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  2:940590/1‑140 (MQ=255)
                            tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTAAGGCTCa                                                                                                          >  2:75090/1‑140 (MQ=255)
                            tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCAAAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  2:408201/1‑140 (MQ=255)
                            tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCAGCAACATTTTATTCGTCATCTGACGGATCGCATACCCGTCACGGTTATCTTCCTCAGtctcc                                                                                                          >  1:18138/1‑139 (MQ=255)
                             cccGTGTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCACCATTTTTTTCGTCATCTGACGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCaa                                                                                                         >  1:1160626/1‑140 (MQ=255)
                              cctttttGTTCATAGCCAAACGCGGCCGTCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  1:2110496/137‑1 (MQ=255)
                              ccGTTTTTTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  1:1026631/140‑1 (MQ=255)
                              ccGTTTTGTTCATAGCCAAACGCTGCCGGGTCTACATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  1:1390657/140‑1 (MQ=255)
                              ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  2:1977719/140‑1 (MQ=255)
                              ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  2:988396/140‑1 (MQ=255)
                              ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  1:2066292/140‑1 (MQ=255)
                                   ttGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGTCTCAAGCTGCa                                                                                                   >  2:2022858/1‑140 (MQ=255)
                                      ttCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATaa                                                                                                >  2:1412855/1‑140 (MQ=255)
                                           aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                           >  1:229607/1‑140 (MQ=255)
                                           aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                           >  2:1252849/1‑140 (MQ=255)
                                           aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                           >  2:1859660/1‑140 (MQ=255)
                                                aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                                      <  1:2035123/140‑1 (MQ=255)
                                                aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                                      <  1:2076022/140‑1 (MQ=255)
                                                aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                                      <  1:1499372/140‑1 (MQ=255)
                                                     ggccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCct                                                                                 <  2:1123135/140‑1 (MQ=255)
                                                      gccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                                <  2:538170/140‑1 (MQ=255)
                                                       ccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctct                                                                               <  1:1690574/140‑1 (MQ=255)
                                                       ccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctct                                                                               >  1:1599346/1‑140 (MQ=255)
                                                               ccATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTaa                                                                       <  2:437198/140‑1 (MQ=255)
                                                                      aaaaaaCGATCAAGAACTTTTGCGCCTCGCTGCGCTTCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATgatgat                                                                <  2:703985/140‑1 (MQ=255)
                                                                      aaaaaaCGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATgatgat                                                                <  2:459298/140‑1 (MQ=255)
                                                                                cAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGaa                                                      <  2:229607/140‑1 (MQ=255)
                                                                                 aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                                     <  2:2203861/140‑1 (MQ=255)
                                                                                 aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                                     <  2:170950/140‑1 (MQ=255)
                                                                                 aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                                     <  2:802680/140‑1 (MQ=255)
                                                                                    aaCTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAAc                                                  <  1:905865/140‑1 (MQ=255)
                                                                                             gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                                         <  2:2307467/140‑1 (MQ=255)
                                                                                             gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                                         <  1:40514/140‑1 (MQ=255)
                                                                                             gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTGATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                                         <  2:1220001/140‑1 (MQ=255)
                                                                                                      cgctgcAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTtgg                                <  1:1755670/140‑1 (MQ=255)
                                                                                                           cAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTtggctggc                           >  2:1298388/1‑140 (MQ=255)
                                                                                                              cATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                                >  1:1456202/1‑84 (MQ=255)
                                                                                                              cATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                                <  2:1456202/84‑1 (MQ=255)
                                                                                                              cATTTTTTTCGTCATCTGCCGGATCGCATAGCCCTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCGCTTGTCTTAATGAGGCTTGAAAGGGAATACCCGTATTGAAATAGTTTGGCTGGCTTg                        >  2:1658619/1‑140 (MQ=255)
                                                                                                                   ttttCGTCATCTGCCGGATCGCCTAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCg                   >  2:1564703/1‑140 (MQ=255)
                                                                                                                   ttttCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGTCTGGCTTGTTTCg                   >  2:2098966/1‑140 (MQ=255)
                                                                                                                     ttCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGCTGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGt                 <  1:1175344/140‑1 (MQ=255)
                                                                                                                         tCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCa             <  1:1213706/140‑1 (MQ=255)
                                                                                                                         tCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGAGTGAAAGGGAATAACAGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCa             >  2:1149028/1‑140 (MQ=255)
                                                                                                                          cATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCAt            <  2:1375246/140‑1 (MQ=255)
                                                                                                                          cATCTGCCGGATCGCAGAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCAt            <  2:34980/140‑1 (MQ=255)
                                                                                                                                    aTCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCATCGTTTGTAtc  <  1:1252849/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TCAATAACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCATCGTTTGTATC  >  NZ_CP034943/2117045‑2117316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑