Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,117,185 G→C 6.2% G531G (GGC→GGG)  EO946_RS10610 ← nitric oxide reductase activation protein NorD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,117,1850GC 6.2% 247.2 / 4.3 77G531G (GGC→GGG) EO946_RS10610nitric oxide reductase activation protein NorD
Reads supporting (aligned to +/- strand):  ref base G (28/44);  new base C (3/2);  total (31/46)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCATCGTTTGTATC  >  NZ_CP034943/2117050‑2117316
                                                                                                                                       |                                                                                                                                   
aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                                 <  2:1082038/140‑1 (MQ=255)
aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                                 <  2:1758128/140‑1 (MQ=255)
aaCCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGt                                                                                                                                 <  2:1875635/140‑1 (MQ=255)
  ccGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                               <  1:1283397/140‑1 (MQ=255)
   cGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCAc                                                                                                                              <  1:1742157/140‑1 (MQ=255)
     cTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCACCATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACgg                                                                                                                            <  2:1280223/140‑1 (MQ=255)
     cTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACgg                                                                                                                            <  1:2022080/140‑1 (MQ=255)
       tCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGtt                                                                                                                          >  1:727950/1‑140 (MQ=255)
       tCACTTGTATCGACAAACCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGtt                                                                                                                          >  2:1690574/1‑140 (MQ=255)
         aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                                        <  2:2146185/140‑1 (MQ=255)
         aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                                        >  2:40514/1‑140 (MQ=255)
         aCTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTAt                                                                                                                        <  1:638022/140‑1 (MQ=255)
          cTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATc                                                                                                                       <  2:2307263/140‑1 (MQ=255)
          agtggaTCGACAATCCCGTTTTCTTCAAAGCCAAACGGGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACATTGGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCCTCTGCCGGATCGCATAGCCGTCACGGTTATc                                                                                                                       <  2:641033/135‑1 (MQ=255)
             gTATCGACAATCCCGTTTTGTTCATAGCCAAAAGCGGCCGGCTCTCCATCTGAAAAAACGATCAATAACTTTTGCGCCTCGCTGAGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTc                                                                                                                    >  2:1755670/1‑140 (MQ=255)
               agcGACAATCCCGGTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGGTGCAACATGTTTTTCGTCATCTGCCGGCTCGCATCGCCGTCCCGGTAATCTTccg                                                                                                                  >  2:528556/3‑139 (MQ=255)
               aTCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCt                                                                                                                  <  2:1108780/140‑1 (MQ=255)
                tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                               >  1:2381024/1‑126 (MQ=255)
                tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCa                                                                                                                               <  2:2381024/126‑1 (MQ=255)
                tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                                 >  1:2288289/1‑140 (MQ=255)
                tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                                 >  1:1777856/1‑140 (MQ=255)
                tCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTc                                                                                                                 >  1:2033516/1‑140 (MQ=255)
                   acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                                       >  2:45862/4‑131 (MQ=255)
                   acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                                       >  2:1707414/4‑131 (MQ=255)
                   acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAATTGCGTACCCGTCACGGTTATc                                                                                                                       <  1:45862/128‑1 (MQ=255)
                   acgaTCCCATTTTGTTCGTAGCCAAATGCGGCCGGCTCTCCATCTGAGAAAACAATTAAGAACTTTTGCGCCTCGCTGCGATGCAGCATTTTTTTCGTCATCTGCCTAAGTGCGTACCCGTCACGGTTATc                                                                                                                       <  1:1707414/128‑1 (MQ=255)
                       tCCCGTTTTGTTCATATCCAAACGCGGCAGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCAGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  2:2378942/1‑140 (MQ=255)
                       tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          <  2:1452790/140‑1 (MQ=255)
                       tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  2:2007602/1‑140 (MQ=255)
                       tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  1:1977719/1‑140 (MQ=255)
                       tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  2:940590/1‑140 (MQ=255)
                       tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTAAGGCTCa                                                                                                          >  2:75090/1‑140 (MQ=255)
                       tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCAAAGCCGTCACGGTTATCTTCCTCAGGCTCa                                                                                                          >  2:408201/1‑140 (MQ=255)
                       tCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCAGCAACATTTTATTCGTCATCTGACGGATCGCATACCCGTCACGGTTATCTTCCTCAGtctcc                                                                                                          >  1:18138/1‑139 (MQ=255)
                        cccGTGTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCACCATTTTTTTCGTCATCTGACGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCaa                                                                                                         >  1:1160626/1‑140 (MQ=255)
                         cctttttGTTCATAGCCAAACGCGGCCGTCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  1:2110496/137‑1 (MQ=255)
                         ccGTTTTTTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  1:1026631/140‑1 (MQ=255)
                         ccGTTTTGTTCATAGCCAAACGCTGCCGGGTCTACATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  1:1390657/140‑1 (MQ=255)
                         ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  2:1977719/140‑1 (MQ=255)
                         ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  2:988396/140‑1 (MQ=255)
                         ccGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAg                                                                                                        <  1:2066292/140‑1 (MQ=255)
                              ttGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGTCTCAAGCTGCa                                                                                                   >  2:2022858/1‑140 (MQ=255)
                                 ttCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATaa                                                                                                >  2:1412855/1‑140 (MQ=255)
                                      aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                           >  1:229607/1‑140 (MQ=255)
                                      aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                           >  2:1859660/1‑140 (MQ=255)
                                      aGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGcc                                                                                           >  2:1252849/1‑140 (MQ=255)
                                           aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                                      <  1:1499372/140‑1 (MQ=255)
                                           aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                                      <  1:2035123/140‑1 (MQ=255)
                                           aaCGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTcc                                                                                      <  1:2076022/140‑1 (MQ=255)
                                                ggccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCct                                                                                 <  2:1123135/140‑1 (MQ=255)
                                                 gccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                                <  2:538170/140‑1 (MQ=255)
                                                  ccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctct                                                                               >  1:1599346/1‑140 (MQ=255)
                                                  ccggcTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctct                                                                               <  1:1690574/140‑1 (MQ=255)
                                                          ccATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTaa                                                                       <  2:437198/140‑1 (MQ=255)
                                                                 aaaaaaCGATCAAGAACTTTTGCGCCTCGCTGCGCTTCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATgatgat                                                                <  2:703985/140‑1 (MQ=255)
                                                                 aaaaaaCGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATgatgat                                                                <  2:459298/140‑1 (MQ=255)
                                                                           cAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGaa                                                      <  2:229607/140‑1 (MQ=255)
                                                                            aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                                     <  2:170950/140‑1 (MQ=255)
                                                                            aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                                     <  2:2203861/140‑1 (MQ=255)
                                                                            aaGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAAt                                                     <  2:802680/140‑1 (MQ=255)
                                                                               aaCTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAAc                                                  <  1:905865/140‑1 (MQ=255)
                                                                                        gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                                         <  2:2307467/140‑1 (MQ=255)
                                                                                        gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                                         <  1:40514/140‑1 (MQ=255)
                                                                                        gcCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTGATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGaa                                         <  2:1220001/140‑1 (MQ=255)
                                                                                                 cgctgcAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTtgg                                <  1:1755670/140‑1 (MQ=255)
                                                                                                      cAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTtggctggc                           >  2:1298388/1‑140 (MQ=255)
                                                                                                         cATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                                <  2:1456202/84‑1 (MQ=255)
                                                                                                         cATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCctc                                                                                >  1:1456202/1‑84 (MQ=255)
                                                                                                         cATTTTTTTCGTCATCTGCCGGATCGCATAGCCCTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCGCTTGTCTTAATGAGGCTTGAAAGGGAATACCCGTATTGAAATAGTTTGGCTGGCTTg                        >  2:1658619/1‑140 (MQ=255)
                                                                                                              ttttCGTCATCTGCCGGATCGCCTAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCg                   >  2:1564703/1‑140 (MQ=255)
                                                                                                              ttttCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGTCTGGCTTGTTTCg                   >  2:2098966/1‑140 (MQ=255)
                                                                                                                ttCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGCTGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGt                 <  1:1175344/140‑1 (MQ=255)
                                                                                                                    tCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCa             <  1:1213706/140‑1 (MQ=255)
                                                                                                                    tCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGAGTGAAAGGGAATAACAGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCa             >  2:1149028/1‑140 (MQ=255)
                                                                                                                     cATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCAt            <  2:1375246/140‑1 (MQ=255)
                                                                                                                     cATCTGCCGGATCGCAGAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCAt            <  2:34980/140‑1 (MQ=255)
                                                                                                                               aTCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCATCGTTTGTAtc  <  1:1252849/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                   
AACCGCTTCACTTGTATCGACAATCCCGTTTTGTTCATAGCCAAACGCGGCCGGCTCTCCATCTGAAAAAACGATCAAGAACTTTTGCGCCTCGCTGCGCTGCAACATTTTTTTCGTCATCTGCCGGATCGCATAGCCGTCACGGTTATCTTCCTCAGGCTCAAGCTGCATAATTGCCGGTCCAGCCTCTTGTCTTAATGATGATTGAAAGGGAATAACCGTATTGAAATAGTTTGGCTGGCTTGTTTCGGTCGCATCGTTTGTATC  >  NZ_CP034943/2117050‑2117316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑