Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,135,516 C→A 7.9% A496E (GCA→GAA)  EO946_RS10715 → sodium:solute symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,135,5160CA 7.9% 243.5 / 8.5 83A496E (GCA→GAA) EO946_RS10715sodium:solute symporter
Reads supporting (aligned to +/- strand):  ref base C (47/29);  new base A (3/4);  total (50/33)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGGTTCTGGGGATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCGTTGTTGTATCG  >  NZ_CP034943/2135378‑2135654
                                                                                                                                          |                                                                                                                                          
aaGGGTTCTGGGGATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCa                                                                                                                                           >  1:1558261/1‑140 (MQ=255)
     ttCTGGGGATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATaaaa                                                                                                                                      <  1:2137587/140‑1 (MQ=255)
       cTGGGGATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATaaaaaa                                                                                                                                    >  1:1007517/1‑140 (MQ=255)
       cTGGGGATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATaaaaaa                                                                                                                                    >  1:386566/1‑140 (MQ=255)
       cTGGGGATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATaaaaaa                                                                                                                                    >  1:691579/1‑140 (MQ=255)
           ggATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCa                                                                                                                                <  1:1966931/140‑1 (MQ=255)
           ggATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCa                                                                                                                                <  1:1965302/140‑1 (MQ=255)
           ggATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCa                                                                                                                                <  1:1004069/140‑1 (MQ=255)
             aTTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAATAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAATATGCTGACCCAAATAAAAAGGGTGAATAAAAAAACCATa                                                                                                                              >  2:1593803/1‑140 (MQ=255)
             aTTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACAATa                                                                                                                              >  1:2030667/1‑140 (MQ=255)
             aTTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGAATAAAAAAACCATa                                                                                                                              >  2:329333/1‑140 (MQ=255)
             aTTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGAATAAAAAAACCATa                                                                                                                              >  2:1593756/1‑140 (MQ=255)
             aTTGGCCGCCAACATCATCACTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATa                                                                                                                              <  1:1022415/140‑1 (MQ=255)
               tGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCCGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATAcg                                                                                                                            >  2:1352568/1‑140 (MQ=255)
               tGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATAcg                                                                                                                            >  2:2135590/1‑140 (MQ=255)
                  ccgccAACCTAATCGCTTTGGTTATTCTGAATCCGCTTTTCGTCAAGAAGGCAGGATCCAATCCTTTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGAATAAAAAAACCATACGCgg                                                                                                                         <  1:1165042/140‑1 (MQ=255)
                    gccAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGcc                                                                                                                       >  1:1932610/1‑139 (MQ=255)
                    gccAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCa                                                                                                                       >  1:1161341/1‑140 (MQ=255)
                          atcatcGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCcgcg                                                                                                                 <  2:1876877/140‑1 (MQ=255)
                              tcGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATg                                                                                                             >  1:823680/1‑140 (MQ=255)
                                         aTTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAAACATACGCGGCAGCCGCGTATGGTTTTTTTTAc                                                                                                  >  1:1906877/1‑140 (MQ=255)
                                                 tCCGCTGTTCGTCATGTAGGAAGGATCCAATCCTGTCATTGAAGGTTTAGTTGGTAAAAAACAAGATGCTGACGCAAATCAAAATGGTGCATAAACAAACCATAGGCGGCAGCCGCGTATGGTTTTTTTTAGAtttttgt                                                                                          >  2:1288541/1‑136 (MQ=255)
                                                 tCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCCCGTATGGTTTTTTTTACATTTCttt                                                                                          >  2:373022/1‑140 (MQ=255)
                                                    gCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAATGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCtatttt                                                                                       <  2:901336/140‑1 (MQ=255)
                                                    gCTGTTCGTCAACACGGCAGGATCCAATCATTTAATTGAAGTTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCtttttt                                                                                       <  2:2114651/140‑1 (MQ=39)
                                                     cTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCCTTGAAGGTTTAGTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATCGGCGGCAGCCGCGTAGGGTTTTTTTTAGATTTCTTTTTTc                                                                                      >  1:1970282/1‑140 (MQ=255)
                                                     cTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTc                                                                                      >  1:1895453/1‑140 (MQ=255)
                                                     cTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTc                                                                                      >  1:2136272/1‑140 (MQ=255)
                                                     cTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGGATGGTTTTTTTTACATTTCTTTTTTc                                                                                      >  1:1494828/1‑140 (MQ=255)
                                                          cGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGt                                                                                 <  2:2136272/140‑1 (MQ=255)
                                                                      aGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCtt                                                                     <  1:496081/140‑1 (MQ=255)
                                                                          tCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTcc                                                                 >  1:1044392/1‑140 (MQ=255)
                                                                            cAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCAt                                                               <  2:1075091/140‑1 (MQ=255)
                                                                            cAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCAt                                                               <  1:2323415/140‑1 (MQ=255)
                                                                            cAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCAt                                                               <  2:353467/140‑1 (MQ=255)
                                                                                 cTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATTGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCg                                                          <  2:1933256/140‑1 (MQ=255)
                                                                                 cTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCg                                                          <  2:259179/140‑1 (MQ=255)
                                                                                 cTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCg                                                          <  2:1048800/140‑1 (MQ=255)
                                                                                  tGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGt                                                         >  2:1279534/1‑140 (MQ=255)
                                                                                    tCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTAt                                                       >  2:2405457/1‑140 (MQ=255)
                                                                                    tCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTAt                                                       >  1:1373987/1‑140 (MQ=255)
                                                                                    tCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTAt                                                       <  2:2153394/140‑1 (MQ=255)
                                                                                    tCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTAt                                                       <  2:1186679/140‑1 (MQ=255)
                                                                                    tCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTAt                                                       >  2:1778509/1‑140 (MQ=255)
                                                                                    tCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTAt                                                       <  1:2227932/140‑1 (MQ=255)
                                                                                        tGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAATAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCatt                                                   >  2:1097730/1‑140 (MQ=255)
                                                                                        tGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCatt                                                   >  2:1045820/1‑140 (MQ=255)
                                                                                              tttATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGTGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGa                                             >  2:1060227/1‑140 (MQ=255)
                                                                                                tATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACa                                           >  2:1538857/1‑140 (MQ=255)
                                                                                                 aTTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTAAGGTTTTTTGTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATAATTAttttagag                                          >  2:1609104/1‑135 (MQ=255)
                                                                                                       tAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGcttctt                                    >  1:1707934/1‑140 (MQ=255)
                                                                                                       tAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGctgctt                                    >  1:687036/1‑140 (MQ=255)
                                                                                                         aaaaaCAAGATGCTGACCCCAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCtttt                                  <  2:1087137/140‑1 (MQ=255)
                                                                                                             aCAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAg                              <  2:2180295/140‑1 (MQ=255)
                                                                                                             aCAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAg                              <  2:1162580/140‑1 (MQ=255)
                                                                                                               aaGATGCTGACCCAACTCAAAAGGGTGCATAAAAAAACCAGACGCCGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGtt                            <  1:2109685/140‑1 (MQ=255)
                                                                                                               aaGATGCTGACCCAAATCAAAAGGCTGCATAAAAAAACCAGACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGtt                            <  1:699342/140‑1 (MQ=255)
                                                                                                                 gATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCt                          >  1:2105335/1‑140 (MQ=255)
                                                                                                                 gATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCt                          >  1:1803625/1‑140 (MQ=255)
                                                                                                                 gATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCt                          >  1:471939/1‑140 (MQ=255)
                                                                                                                 gATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTAGGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCt                          >  1:1944164/1‑140 (MQ=255)
                                                                                                                      tGACCCAAATCAAAAGGGTGCATAAAAAACCCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGGTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCAt                     <  1:1018829/140‑1 (MQ=255)
                                                                                                                       gACCCCCATCAAAAGGGTGCATAACAAAACCCTCCGCGGCAGCCGCGTATGGTTTTTTTTACATTTCATTTTTCAGAGTTTCGATTGCCTTTTCCTTTTGCGTATCATTATTTGCCAGCTTCTTTTGAAGTTCTGTCAtt                    <  2:2313276/140‑1 (MQ=255)
                                                                                                                       gACCCAAATCAAAAGGGTGCATAAAAACACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCAtt                    <  2:2261981/140‑1 (MQ=255)
                                                                                                                         cccAAATCAAAAGGGTGAATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTaa                  <  1:1593756/140‑1 (MQ=255)
                                                                                                                         cccAAATCAAAAGGGTGAATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTaa                  <  1:329333/140‑1 (MQ=255)
                                                                                                                         cacAAATCAAAAGTCTGAATAAAAAAACCATACGCGGCAGCCGCGTATGTTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGACTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTaa                  <  1:1593803/138‑1 (MQ=255)
                                                                                                                             aaTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTCTGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTc              >  2:2093860/1‑140 (MQ=255)
                                                                                                                             aaTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTc              >  1:961041/1‑140 (MQ=255)
                                                                                                                             aaTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTc              >  1:68670/1‑140 (MQ=255)
                                                                                                                             aaTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTc              >  1:1312198/1‑140 (MQ=255)
                                                                                                                             aaTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTc              >  1:105992/1‑140 (MQ=255)
                                                                                                                             aaTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCGGTCATTAATTTc              >  2:883888/1‑140 (MQ=255)
                                                                                                                             aaTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGGATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTc              >  1:68685/1‑140 (MQ=255)
                                                                                                                              aTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCAt                                                               >  2:115919/1‑90 (MQ=255)
                                                                                                                              aTCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCAt                                                               <  1:115919/90‑1 (MQ=255)
                                                                                                                                  aaaGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCgttgt         >  2:1110551/1‑140 (MQ=255)
                                                                                                                                   aaGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCgttgtt        >  2:1552761/1‑140 (MQ=255)
                                                                                                                                    aGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCGttgttg       >  2:2001585/1‑140 (MQ=255)
                                                                                                                                    aGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCGttgttg       >  2:929709/1‑140 (MQ=255)
                                                                                                                                    aGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCGttgttg       >  2:627201/1‑140 (MQ=255)
                                                                                                                                    aGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTACCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCGttgttg       >  2:512516/1‑140 (MQ=255)
                                                                                                                                         gCATAAAAAAACCATTCGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCATAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCGTTGTTGTATCg  <  2:564509/140‑1 (MQ=255)
                                                                                                                                          cATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCat                                                    >  2:969525/1‑89 (MQ=255)
                                                                                                                                          cATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCat                                                    >  2:1923500/1‑89 (MQ=255)
                                                                                                                                          cATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCat                                                    <  1:969525/89‑1 (MQ=255)
                                                                                                                                          cATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCat                                                    <  1:1923500/89‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AAGGGTTCTGGGGATTGGCCGCCAACATCATCGCTGTGGTTATTCTGAATCCGCTGTTCGTCAAGAAGGCAGGATCCAATCCTGTCATTGAAGGTTTATTTGGTAAAAAACAAGATGCTGACCCAAATCAAAAGGGTGCATAAAAAAACCATACGCGGCAGCCGCGTATGGTTTTTTTTACATTTCTTTTTTCAGAGTTTCGATTGCCTTTTCCATTTGCGTATCATTATTTGACAGCTTCTTTTGAAGTTCTGTCATTAATTTCGTTGTTGTATCG  >  NZ_CP034943/2135378‑2135654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑