BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,145,766	A→G	5.1%	L309L (TTG→CTG)	EO946_RS10800 ←	peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,145,766	0	A	G	5.1%	565.7 / 13.5	176	L309L (TTG→CTG)	EO946_RS10800	peptidase
Reads supporting (aligned to +/- strand): ref base A (86/78); new base G (5/4); total (91/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.44e-01

Only 100 of 177 total aligned reads displayed.

GTTTTTTGATAGGCTGATTGCAGCACTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCG  >  NZ_CP034943/2145627‑2145903

gTTTTTTGATAGGCTGATTGCAGCACTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCaa                                                                                                                                           >  2:2335767/1‑140 (MQ=255)

         tAGGCTGATTGCAGCACTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTc                                                                                                                                  <  2:1661965/140‑1 (MQ=255)

         tAGGCTGATTGCAGCACTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGACGATCCATTCTACTGTTACCGGATGGCGTTTAAAACATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTc                                                                                                                                  <  1:390588/140‑1 (MQ=255)

           ggCTGATTGCAGCACTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCa                                                                                                                                >  1:1725538/1‑140 (MQ=255)

               gATTGCAGCACTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTc                                                                                                                            <  1:874505/140‑1 (MQ=255)

                aTTGCAGCACTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCa                                                                                                                           <  1:1704514/140‑1 (MQ=255)

                      gcaCTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGtttt                                                                                                                     >  2:1561457/1‑140 (MQ=255)

                       caCTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTa                                                                                                                    >  1:1919791/1‑140 (MQ=255)

                       caCTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTa                                                                                                                    <  1:227099/140‑1 (MQ=255)

                       caCTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTa                                                                                                                    >  2:68387/1‑140 (MQ=255)

                       caCTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTa                                                                                                                    >  2:1120701/1‑140 (MQ=255)

                       caCTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTa                                                                                                                    >  2:133602/1‑140 (MQ=255)

                       caCTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGCTATTCTAAATCCTTCAACCAGTTTTCCAATTAAATTTTTa                                                                                                                    >  2:1827048/1‑140 (MQ=255)

                            aaaTTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCt                                                                                                               >  1:282759/1‑140 (MQ=255)

                                 aaaGGGTGGTCATCAGTTAAATCATACGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGCATGGCGTTTAAACCATTCTTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTGTgg                                                                                                          >  2:335703/1‑140 (MQ=255)

                                        ggtCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCa                                                                                                   >  1:63664/1‑140 (MQ=255)

                                         gtCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTc                                                                                                                            >  1:295491/1‑114 (MQ=255)

                                         gtCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTc                                                                                                                            <  2:295491/114‑1 (MQ=255)

                                          tcatcaGGTAACTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGGTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCACCCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATg                                                                                                 <  2:1915173/140‑1 (MQ=255)

                                          tcatcaGGAAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTGTCCACTTCAACTTTTACTGCTTCTGGTTTTTCATg                                                                                                 <  2:2361757/140‑1 (MQ=255)

                                                ggTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTAc                                                                                                                   >  1:999526/1‑116 (MQ=255)

                                                   aaaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCATTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAt                                                                                        >  2:1607178/1‑140 (MQ=255)

                                                    aataatTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTGCTGGTTTTTCATGGGGAGCACtt                                                                                       >  2:1308195/1‑140 (MQ=255)

                                                    aaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCCAtt                                                                                       >  2:2062667/1‑140 (MQ=255)

                                                    aaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAtt                                                                                       >  2:2374465/1‑140 (MQ=255)

                                                    aaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAtt                                                                                       >  2:2085683/1‑140 (MQ=255)

                                                       cATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                    <  2:1550789/140‑1 (MQ=255)

                                                           ctgCAGCCATTGAGCGCCAAACCATTCGACTTGTACGGGATTGCGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCAtc                                                                                >  2:280235/3‑140 (MQ=255)

                                                           cGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCTTGGGGAGCAATTCCGCAtc                                                                                >  2:283593/1‑140 (MQ=255)

                                                           cGGCAGCCATTGAGCGCCAAACCATTCGACTTGTACGGGATGGCGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCAtc                                                                                >  2:1485489/1‑140 (MQ=255)

                                                            ggCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAtct                                                                               <  2:1158835/140‑1 (MQ=255)

                                                             gCAGCCATTGAGCGCCGAACCATTCTACTGGTACCGGATGGCGGTTAAACCATTCGTCACGGTATTCTCACTCCTTCACCCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAtctc                                                                              <  2:760803/140‑1 (MQ=255)

                                                             gCAGCCATTGAGCGCCGAACCATTCTACGGTGACCGGATGGCGTTTAAACCAGTCGTCACGGTAGTCTAACTCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAtctc                                                                              <  2:760849/140‑1 (MQ=255)

                                                                  cATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTc                                                                         <  1:487109/140‑1 (MQ=255)

                                                                         cgcCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATgacg                                                                  >  2:2431451/1‑140 (MQ=255)

                                                                           ccGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATgacgac                                                                >  2:403382/1‑140 (MQ=255)

                                                                           ccGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATgacgac                                                                >  2:2320166/1‑140 (MQ=255)

                                                                            cGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                               <  2:2193221/140‑1 (MQ=255)

                                                                              aaCCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACg                                                             <  2:1766865/140‑1 (MQ=255)

                                                                              aaCCATTCTACTGGTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACg                                                             <  2:1623836/140‑1 (MQ=255)

                                                                                        cTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACt                                                   >  2:704293/1‑140 (MQ=255)

                                                                                           ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                >  2:514272/1‑140 (MQ=255)

                                                                                           ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                >  2:633477/1‑140 (MQ=255)

                                                                                           ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                <  2:643401/140‑1 (MQ=255)

                                                                                           ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                <  2:1083494/140‑1 (MQ=255)

                                                                                           ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAGTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                <  2:1203356/140‑1 (MQ=255)

                                                                                            tACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACg                                               <  1:470833/140‑1 (MQ=255)

                                                                                             aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                               <  2:47811/123‑1 (MQ=255)

                                                                                             aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                               >  1:1829292/1‑123 (MQ=255)

                                                                                             aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                               <  2:1829292/123‑1 (MQ=255)

                                                                                             aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGa                                              >  1:1258667/1‑140 (MQ=255)

                                                                                             aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGa                                              >  1:1382556/1‑140 (MQ=255)

                                                                                             aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGa                                              >  1:222104/1‑140 (MQ=255)

                                                                                                 gATGGCTTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGc                                          <  1:1944038/140‑1 (MQ=255)

                                                                                                  aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTAGTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                         >  1:2078761/1‑140 (MQ=255)

                                                                                                  aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                         >  1:56787/1‑140 (MQ=255)

                                                                                                  aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                         >  1:1746305/1‑140 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      <  2:1687222/140‑1 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      <  2:1799507/140‑1 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      <  2:933183/140‑1 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      <  1:1127293/140‑1 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      >  1:1226584/1‑140 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      >  1:697726/1‑140 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      >  1:172081/1‑140 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      <  1:275624/140‑1 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTGTTACTGCTTCTGGTTTTTCATGGGGATCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      >  1:386097/1‑140 (MQ=255)

                                                                                                     gCGTTTAAACCATTCGTCACGGTAGTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                      >  1:2372261/1‑140 (MQ=255)

                                                                                                      cGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGAAGATGGCCAGGc                                     <  1:1485489/140‑1 (MQ=255)

                                                                                                      cGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGc                                     <  1:430897/140‑1 (MQ=255)

                                                                                                       gTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCt                                    <  1:525966/140‑1 (MQ=255)

                                                                                                            aaCCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTccccc                               <  2:1947167/140‑1 (MQ=255)

                                                                                                                aTTCGTCCCGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGATTTTCATGGGGAGCAATTCCGCATCTCACTTCTATGACGACACGATCTGCAAATGACGAAGGCCACGCTCCCCCTgag                           >  2:991825/1‑140 (MQ=255)

                                                                                                                aTTCGTCACGGTATTCTAAATCCTTCAAGCAGGTTTCCAATTCAAGTTTTAGTGCGTCTGGTTTTTCTTGGGGAGCAATTCCGCTTCTCCCTTCTCTGACGACCCGATCTGGAACTGACGAAGGCCACGCTCCCCCTgag                           >  1:1922674/1‑140 (MQ=255)

                                                                                                                aTTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgag                           >  1:2132935/1‑140 (MQ=255)

                                                                                                                 ttCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGAAGATGGCCAGGCACCGCCTgaga                          >  1:2169949/1‑140 (MQ=255)

                                                                                                                 ttCGTCACGGTATTCTACATCCTTCAACCACTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                          <  1:1624418/140‑1 (MQ=255)

                                                                                                                 ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                          >  2:400560/1‑140 (MQ=255)

                                                                                                                 ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                          <  1:1112255/140‑1 (MQ=255)

                                                                                                                 ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                          >  2:1456779/1‑140 (MQ=255)

                                                                                                                 ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                          >  2:1301519/1‑140 (MQ=255)

                                                                                                                  tCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                    >  1:2026326/1‑81 (MQ=255)

                                                                                                                  tCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                    <  2:2026326/81‑1 (MQ=255)

                                                                                                                       aCGGTATTCTAAATCCTACAACCAGTTTTCCAATTCAAGATTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGc                    <  1:1875785/140‑1 (MQ=255)

                                                                                                                         ggTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCg                  >  2:1896035/1‑140 (MQ=255)

                                                                                                                           tATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGat                >  1:2325757/1‑140 (MQ=255)

                                                                                                                           tATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGat                >  1:1806223/1‑140 (MQ=255)

                                                                                                                             ttCTAAATCCTTCACCAAGTTTTCCAATTCAAGTTTTACTGAGTCTGGTTTTTCCTGGGGAGCAATTCCGCCTCTCCCGTCTAGGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGatat              <  1:1607178/140‑1 (MQ=255)

                                                                                                                             ttCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGACCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGatat              <  1:2185468/140‑1 (MQ=255)

                                                                                                                                tAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCa           >  2:1741117/1‑140 (MQ=255)

                                                                                                                                   aTCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTg        >  2:83462/1‑140 (MQ=255)

                                                                                                                                       ttCAACGAGTTTTCCACTTCAAGTGTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc    <  1:133602/140‑1 (MQ=255)

                                                                                                                                       ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc    >  1:315270/1‑140 (MQ=255)

                                                                                                                                       ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc    >  2:614381/1‑140 (MQ=255)

                                                                                                                                       ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc    >  1:1291846/1‑140 (MQ=255)

                                                                                                                                       ttCAACCAGTTTTCCAATTCAAGTTGTACTGCTTCTTGTTTTTCATGGGTAGCAATTCCGCATCTGCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc    <  2:1479297/140‑1 (MQ=255)

                                                                                                                                       ttCAACCAGTTTTCCAATTCAAGTGTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATAGTCACTGGTAc    >  1:1515436/1‑140 (MQ=255)

                                                                                                                                        tCAACCAGTTTTCCAATTCCAGTTTTACTGCTTCTGGATTTTCATGGGGAGCAATTCCCCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc   <  1:1566297/140‑1 (MQ=255)

                                                                                                                                        tCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc   <  2:2132935/140‑1 (MQ=255)

                                                                                                                                        tCAACCAGTTTTCATATTCAAGTTTTAATGCTTCTGGTTTTTCATGGGGTGCTATTCCGCATCTCCCTTCTATGTCGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc   <  1:1795754/140‑1 (MQ=255)

                                                                                                                                         cAACCAGTTTTCCAATTCCAGTTTTACTGCTTCGGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGCAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCg  >  1:116725/1‑140 (MQ=255)

GTTTTTTGATAGGCTGATTGCAGCACTGAAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCG  >  NZ_CP034943/2145627‑2145903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`