BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,145,791	T→A	5.0%	A300A (GCA→GCT)	EO946_RS10800 ←	peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,145,791	0	T	A	5.0%	602.4 / 17.1	180	A300A (GCA→GCT)	EO946_RS10800	peptidase
Reads supporting (aligned to +/- strand): ref base T (76/95); new base A (5/4); total (81/99)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.53e-01

Only 100 of 180 total aligned reads displayed.

AAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACA  >  NZ_CP034943/2145655‑2145914

     aaaGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTgg                                                                                                                     >  2:1681816/1‑140 (MQ=255)

              tcatcaGGTAACTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGGTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCACCCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATg                                                                                                            <  2:1915173/140‑1 (MQ=255)

              tcatcaGGAAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTGTCCACTTCAACTTTTACTGCTTCTGGTTTTTCATg                                                                                                            <  2:2361757/140‑1 (MQ=255)

                        aaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCCAtt                                                                                                  >  2:2062667/1‑140 (MQ=255)

                        aaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAtt                                                                                                  >  2:2374465/1‑140 (MQ=255)

                           cATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                               <  2:1550789/140‑1 (MQ=255)

                           cATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                               <  2:1725922/140‑1 (MQ=255)

                              tCGGCCGCCATTGAGCGCCGAACCCTTCTCCTGTGACCGGATGGCGATTAAACCATTCGTCACGGTATTCTAAATCCTTCCACCAGTTGTCCAATTCAAGAATTCCTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAt                                                                                            <  2:382029/140‑1 (MQ=255)

                              tCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAt                                                                                            <  1:1880420/140‑1 (MQ=255)

                               ctgCAGCCATTGAGCGCCAAACCATTCGACTTGTACGGGATTGCGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCAtc                                                                                           >  2:280235/3‑140 (MQ=255)

                               cGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTCTTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAtc                                                                                           >  2:284220/1‑140 (MQ=255)

                               cGGCAGCCATTGAGCGCCAAACCATTCGACTTGTACGGGATGGCGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCAtc                                                                                           >  2:1485489/1‑140 (MQ=255)

                                ggCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAtct                                                                                          <  2:1158835/140‑1 (MQ=255)

                                 gCAGCCATTGAGCGCCGAACCATTCTACTGGTACCGGATGGCGGTTAAACCATTCGTCACGGTATTCTCACTCCTTCACCCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAtctc                                                                                         <  2:760803/140‑1 (MQ=255)

                                  cAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTcc                                                                                        >  2:470833/1‑140 (MQ=255)

                                      cATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTc                                                                                    <  1:487459/140‑1 (MQ=255)

                                               ccGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATgacgac                                                                           >  2:2320166/1‑140 (MQ=255)

                                                cGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                                          <  2:2193221/140‑1 (MQ=255)

                                                cGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                                          <  1:2335767/140‑1 (MQ=255)

                                                               ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                           <  2:1083494/140‑1 (MQ=255)

                                                               ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                           >  2:633477/1‑140 (MQ=255)

                                                               ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                           <  2:643401/140‑1 (MQ=255)

                                                               ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                           <  2:793436/140‑1 (MQ=255)

                                                               ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                           <  1:1791411/140‑1 (MQ=255)

                                                               ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAGTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                           <  2:1203356/140‑1 (MQ=255)

                                                               gtACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                           <  2:1466524/139‑1 (MQ=255)

                                                                tACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACg                                                          <  1:470833/140‑1 (MQ=255)

                                                                tACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACg                                                          <  1:1561457/140‑1 (MQ=255)

                                                                tACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACg                                                          >  1:1932632/1‑140 (MQ=255)

                                                                 aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTGTATGACGACACGATCTGCAACTGTCGa                                                         >  1:1600485/1‑140 (MQ=255)

                                                                 aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                                          <  2:1829166/123‑1 (MQ=255)

                                                                 aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                                          >  1:47811/1‑123 (MQ=255)

                                                                 aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                                          >  1:1829292/1‑123 (MQ=255)

                                                                 aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGa                                                         >  1:222104/1‑140 (MQ=255)

                                                                 aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGa                                                         >  1:1467326/1‑140 (MQ=255)

                                                                  cgggATGGCGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGAAGat                                                        >  1:1318591/3‑139 (MQ=255)

                                                                     gATGGCTTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGc                                                     <  1:1944038/140‑1 (MQ=255)

                                                                      aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTAGTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                                    >  1:2078761/1‑140 (MQ=255)

                                                                      aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                                    >  2:735146/1‑140 (MQ=255)

                                                                      aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                                    >  1:1746305/1‑140 (MQ=255)

                                                                      aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                                    >  1:56787/1‑140 (MQ=255)

                                                                       tGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCa                                                   <  1:40121/140‑1 (MQ=255)

                                                                         gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                 <  2:478491/140‑1 (MQ=255)

                                                                         gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                 <  1:683149/140‑1 (MQ=255)

                                                                         gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                 >  1:697726/1‑140 (MQ=255)

                                                                         gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                 >  1:1226584/1‑140 (MQ=255)

                                                                         gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                 <  2:1799507/140‑1 (MQ=255)

                                                                         gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                 >  1:172081/1‑140 (MQ=255)

                                                                         gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                 <  2:1574867/140‑1 (MQ=255)

                                                                         gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTGTTACTGCTTCTGGTTTTTCATGGGGATCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                 >  1:386097/1‑140 (MQ=255)

                                                                          cGTTTAAACCATTCGTCATGTTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGAAGATGGCCAGGc                                                <  1:280235/140‑1 (MQ=255)

                                                                          cGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGaa                                                           <  1:527001/129‑2 (MQ=255)

                                                                          cGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGaa                                                           >  2:527001/1‑128 (MQ=255)

                                                                          cGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGAAGATGGCCAGGc                                                <  1:1485489/140‑1 (MQ=255)

                                                                          cGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGc                                                <  1:1351676/140‑1 (MQ=255)

                                                                           gTTTAATCCTTTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCTTCTCCCTTCTATGAAGACACGATCTGCAACTGACGAAGGCCACGCt                                               <  2:2388068/140‑1 (MQ=255)

                                                                            tttAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTc                                              >  2:698149/1‑140 (MQ=255)

                                                                             ttAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTcc                                             <  1:1873620/140‑1 (MQ=255)

                                                                                aaCCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTccccc                                          <  2:1947167/140‑1 (MQ=255)

                                                                                     ttCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGAAGATGGCCAGGCACCGCCTgaga                                     >  1:2169949/1‑140 (MQ=255)

                                                                                     ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                     >  2:400560/1‑140 (MQ=255)

                                                                                     ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                     <  1:1112255/140‑1 (MQ=255)

                                                                                     ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                     >  2:1855518/1‑140 (MQ=255)

                                                                                     ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                     >  2:1301519/1‑140 (MQ=255)

                                                                                     gtCGTCACGGTATTCTAAATCCGTCAACCAGTGGTCCAATTCAAGTGTGACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                     <  1:1624276/139‑1 (MQ=255)

                                                                                      tCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                               >  1:2026326/1‑81 (MQ=255)

                                                                                      tCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                               <  2:2026326/81‑1 (MQ=255)

                                                                                      tCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGAt                                    >  2:975213/1‑140 (MQ=255)

                                                                                           atggTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGAAGATGGCCAGGCACCGCCTGAGATGGTGc                               <  2:1318591/138‑1 (MQ=255)

                                                                                             ggTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCg                             >  2:1896035/1‑140 (MQ=255)

                                                                                               tATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGat                           >  1:1806223/1‑140 (MQ=255)

                                                                                               tATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGat                           >  1:2325757/1‑140 (MQ=255)

                                                                                                aTTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGata                          <  1:301666/140‑1 (MQ=255)

                                                                                                    tAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCa                      >  2:1741117/1‑140 (MQ=255)

                                                                                                       aTCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTg                   >  1:1161935/1‑140 (MQ=255)

                                                                                                           ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGCTATTCACGGGTAc               >  2:2098839/1‑140 (MQ=255)

                                                                                                           ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc               >  1:2189478/1‑140 (MQ=255)

                                                                                                           ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc               >  1:1291846/1‑140 (MQ=255)

                                                                                                           ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc               >  2:614381/1‑140 (MQ=255)

                                                                                                            tCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc              <  2:2132935/140‑1 (MQ=255)

                                                                                                            tCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc              <  2:907849/140‑1 (MQ=255)

                                                                                                            tCAACCAGTTGTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc              <  2:1382556/140‑1 (MQ=255)

                                                                                                                  aGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTAtt        <  1:916935/140‑1 (MQ=255)

                                                                                                                   gTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGAAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTg       >  2:2431982/1‑140 (MQ=255)

                                                                                                                    ttttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTa          <  2:1628344/136‑1 (MQ=255)

                                                                                                                    ttttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTa          >  1:1628344/1‑136 (MQ=255)

                                                                                                                      ttCCAATTCCAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  2:1607669/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  1:1120640/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  1:1120915/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  1:1238409/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  1:1827048/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  1:2105570/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  2:622348/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  1:400560/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  1:895169/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  2:1559409/140‑1 (MQ=255)

                                                                                                                      ttCCAATTCAAGGTTTACTGCTTCTGCTTTTTCATGGGGAGCCATTCCGCATCTCCCTTCTATGACGACAAGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa    <  1:2373121/140‑1 (MQ=255)

                                                                                                                       tCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGAc   <  1:2177298/140‑1 (MQ=255)

                                                                                                                        ccAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACa  <  1:1625991/140‑1 (MQ=255)

                                                                                                                        ccAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACa  <  1:1168681/140‑1 (MQ=255)

AAATTAAAGGGTGGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACA  >  NZ_CP034943/2145655‑2145914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`