Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,145,803 T→C 5.1% E296E (GAA→GAG EO946_RS10800 ← peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,145,8030TC 5.1% 586.9 / 13.2 179E296E (GAA→GAGEO946_RS10800peptidase
Reads supporting (aligned to +/- strand):  ref base T (73/96);  new base C (5/4);  total (79/100)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 180 total aligned reads displayed.

GGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACATTGTCATACAGCGGATCTGAAATTCTCG  >  NZ_CP034943/2145667‑2145942
                                                                                                                                        |                                                                                                                                           
           aaaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAt                                                                                                                               >  2:2185468/1‑140 (MQ=255)
            aataatTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTGCTGGTTTTTCATGGGGAGCACtt                                                                                                                              >  2:1308195/1‑140 (MQ=255)
            aaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAtt                                                                                                                              >  2:2374465/1‑140 (MQ=255)
            aaTCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAtt                                                                                                                              >  2:2085683/1‑140 (MQ=255)
               cATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                                                           <  2:1725922/140‑1 (MQ=255)
               cATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCg                                                                                                                           <  2:1550789/140‑1 (MQ=255)
                 ttCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCa                                                                                                                         <  2:1554015/140‑1 (MQ=255)
                  tCGGCCGCCATTGAGCGCCGAACCCTTCTCCTGTGACCGGATGGCGATTAAACCATTCGTCACGGTATTCTAAATCCTTCCACCAGTTGTCCAATTCAAGAATTCCTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAt                                                                                                                        <  2:382029/140‑1 (MQ=255)
                  tCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAt                                                                                                                        <  1:203506/140‑1 (MQ=255)
                   cGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCTTGGGGAGCAATTCCGCAtc                                                                                                                       >  2:283593/1‑140 (MQ=255)
                   cGGCAGCCATTGAGCGCCAAACCATTCGACTTGTACGGGATGGCGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCAtc                                                                                                                       >  2:1485489/1‑140 (MQ=255)
                    ggCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAtct                                                                                                                      <  2:1976373/140‑1 (MQ=255)
                     gCAGCCATTGAGCGCCGAACCATTCTACTGGTACCGGATGGCGGTTAAACCATTCGTCACGGTATTCTCACTCCTTCACCCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCAtctc                                                                                                                     <  2:760803/140‑1 (MQ=255)
                      cAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTcc                                                                                                                    >  2:470833/1‑140 (MQ=255)
                          cATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTc                                                                                                                <  1:487459/140‑1 (MQ=255)
                                 cgcCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATgacg                                                                                                         >  2:2431451/1‑140 (MQ=255)
                                 cgcCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTGCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATgacg                                                                                                         >  2:2374126/1‑140 (MQ=255)
                                 cgcCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAAGTCAAGTTTTACTGCTGCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCGTCTATgacg                                                                                                         >  2:1149243/1‑140 (MQ=255)
                                   ccGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATgacgac                                                                                                       >  2:2320191/1‑140 (MQ=255)
                                      aaCCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACg                                                                                                    <  2:1766865/140‑1 (MQ=255)
                                                   ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                                                       <  2:793436/140‑1 (MQ=255)
                                                   ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                                                       <  2:643401/140‑1 (MQ=255)
                                                   ttACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                                                       >  2:633477/1‑140 (MQ=255)
                                                   gtACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAc                                                                                       <  2:1466524/139‑1 (MQ=255)
                                                    tACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACg                                                                                      >  1:1932632/1‑140 (MQ=255)
                                                    tACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACg                                                                                      <  1:470833/140‑1 (MQ=255)
                                                     aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                                                                      >  1:1829166/1‑123 (MQ=255)
                                                     aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                                                                      >  1:47811/1‑123 (MQ=255)
                                                     aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGaca                                                                                                      <  2:1829292/123‑1 (MQ=255)
                                                     aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGa                                                                                     >  1:1382556/1‑140 (MQ=255)
                                                     aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGa                                                                                     >  1:907849/1‑140 (MQ=255)
                                                     aCCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGa                                                                                     >  1:1467326/1‑140 (MQ=255)
                                                         gATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGc                                                                                 <  1:2085683/140‑1 (MQ=255)
                                                          aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                                                                >  1:56787/1‑140 (MQ=255)
                                                          aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                                                                >  2:735146/1‑140 (MQ=255)
                                                          aTGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGGTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCACTTCTATGACGACACGATCTGCAACTGACGAAGGcc                                                                                >  1:73022/1‑140 (MQ=255)
                                                           tGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCa                                                                               <  1:40121/140‑1 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             <  2:1687222/140‑1 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             <  1:683149/140‑1 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             <  2:1574867/140‑1 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             <  1:275624/140‑1 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             >  1:1226584/1‑140 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             <  2:478491/140‑1 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCACTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             >  1:2231156/1‑140 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTGTTACTGCTTCTGGTTTTTCATGGGGATCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             >  1:386097/1‑140 (MQ=255)
                                                             gCGTTTAAACCATTCGTCACGGTAGTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACg                                                                             >  1:2372261/1‑140 (MQ=255)
                                                              cGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGaa                                                                                       <  1:527001/129‑2 (MQ=255)
                                                              cGTTTAAACCATTCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGaa                                                                                       >  2:527001/1‑128 (MQ=255)
                                                               gTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGcg                                                                           <  1:172283/140‑2 (MQ=255)
                                                               gTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCt                                                                           <  2:2035391/140‑1 (MQ=255)
                                                                tttAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTc                                                                          >  2:698149/1‑140 (MQ=255)
                                                                        aTTCGTCACGGTATTCTAAATCCTTCAAGCAGGTTTCCAATTCAAGTTTTAGTGCGTCTGGTTTTTCTTGGGGAGCAATTCCGCTTCTCCCTTCTCTGACGACCCGATCTGGAACTGACGAAGGCCACGCTCCCCCTgag                                                                  >  1:1922674/1‑140 (MQ=255)
                                                                        aTTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgag                                                                  >  1:2132935/1‑140 (MQ=255)
                                                                         ttCGTCATGGTATTCTAAATCCTTCAGCCAGTTTTCCAATTCAAGTTTTACAGCTTCTGGTTTCTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGGTCTGCAACTGAAGATGGCCAGGCACCGCCTgaga                                                                 >  1:2169949/1‑140 (MQ=255)
                                                                         ttCGTCACGGTATTCTACATCCTTCAACCACTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                                                 <  1:1624418/140‑1 (MQ=255)
                                                                         ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                                                 >  2:1855518/1‑140 (MQ=255)
                                                                         ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                                                 >  2:1301519/1‑140 (MQ=255)
                                                                         ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                                                 >  2:400560/1‑140 (MQ=255)
                                                                         ttCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTgaga                                                                 <  1:1112255/140‑1 (MQ=255)
                                                                          tCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGAt                                                                >  2:975213/1‑140 (MQ=255)
                                                                               aCGGTATTCTAAATCCTACAACCAGTTTTCCAATTCAAGATTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGc                                                           <  1:1875785/140‑1 (MQ=255)
                                                                                 ggTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCg                                                         <  1:1681816/140‑1 (MQ=255)
                                                                                   tATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGat                                                       >  1:1806223/1‑140 (MQ=255)
                                                                                   tATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGat                                                       >  1:2325757/1‑140 (MQ=255)
                                                                                   tATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCACGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGat                                                       >  1:1976642/1‑140 (MQ=255)
                                                                                    aTTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGata                                                      <  1:301666/140‑1 (MQ=255)
                                                                                     ttCTAAATCCTTCACCAAGTTTTCCAATTCAAGTTTTACTGAGTCTGGTTTTTCCTGGGGAGCAATTCCGCCTCTCCCGTCTAGGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGatat                                                     <  1:1607178/140‑1 (MQ=255)
                                                                                     ttCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGACCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGatat                                                     <  1:2185468/140‑1 (MQ=255)
                                                                                         aaaTCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAAGTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCAc                                                 <  2:1808979/140‑1 (MQ=255)
                                                                                           aTCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTg                                               >  1:1161935/1‑140 (MQ=255)
                                                                                               ttCAACGAGTTTTCCACTTCAAGTGTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc                                           <  1:133602/140‑1 (MQ=255)
                                                                                               ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTCTGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc                                           <  2:264338/140‑1 (MQ=255)
                                                                                               ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGCTATTCACGGGTAc                                           >  2:2098839/1‑140 (MQ=255)
                                                                                               ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc                                           >  2:614381/1‑140 (MQ=255)
                                                                                               ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc                                           >  1:1291846/1‑140 (MQ=255)
                                                                                               ttCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGAAGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAc                                           >  2:2215629/1‑140 (MQ=255)
                                                                                               ttCAACCAGTTTTCCAATTCAAGTGTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATAGTCACTGGTAc                                           >  1:1515436/1‑140 (MQ=255)
                                                                                                tCAACCAGTTTTCCAATTCCAGTTTTACTGCTTCTGGATTTTCATGGGGAGCAATTCCCCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc                                          <  1:1566297/140‑1 (MQ=255)
                                                                                                tCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc                                          <  2:1919791/140‑1 (MQ=255)
                                                                                                tCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc                                          <  2:907849/140‑1 (MQ=255)
                                                                                                tCAACCAGTTTTCATATTCAAGTTTTAATGCTTCTGGTTTTTCATGGGGTGCTATTCCGCATCTCCCTTCTATGTCGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc                                          <  1:1795754/140‑1 (MQ=255)
                                                                                                tCAACCAGTTGTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTAcc                                          <  2:1382556/140‑1 (MQ=255)
                                                                                                      aGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTAtt                                    <  1:220019/140‑1 (MQ=255)
                                                                                                      aGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTAtt                                    <  1:916935/140‑1 (MQ=255)
                                                                                                        ttttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTa                                      <  2:1628344/136‑1 (MQ=255)
                                                                                                        ttttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTgg                                  >  2:1194639/1‑140 (MQ=255)
                                                                                                        ttttCCAATTCAAGTTTTACTGCTTCTGGTTTATCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTCCCGGTATTgg                                  >  2:1900485/1‑140 (MQ=255)
                                                                                                          ttCCAATTCCAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa                                <  2:1607669/140‑1 (MQ=255)
                                                                                                          ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa                                <  1:1120915/140‑1 (MQ=255)
                                                                                                          ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa                                <  1:1238409/140‑1 (MQ=255)
                                                                                                          ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa                                <  1:1120640/140‑1 (MQ=255)
                                                                                                          ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa                                <  2:622348/140‑1 (MQ=255)
                                                                                                          ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa                                <  1:400560/140‑1 (MQ=255)
                                                                                                          ttCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCGTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGa                                <  1:1750907/140‑1 (MQ=255)
                                                                                                           tCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGAc                               <  1:184234/140‑1 (MQ=255)
                                                                                                            ccAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACa                              <  1:1625991/140‑1 (MQ=255)
                                                                                                            ccAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACa                              <  1:1168681/140‑1 (MQ=255)
                                                                                                                                tCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTCCCGGGCTTGGGACATTGTCATCCAGCGGATCTGa          >  1:1315774/1‑140 (MQ=255)
                                                                                                                                tCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACATTGTCATACAGCGGATCTGa          <  1:690525/140‑1 (MQ=255)
                                                                                                                                        ttCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACATTGTCATACAGCGGATCTGAAATTCTCg  >  2:120750/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
GGTCATCAGGTAAATCATTCGGCAGCCATTGAGCGCCGAACCATTCTACTGTTACCGGATGGCGTTTAAACCATTCGTCACGGTATTCTAAATCCTTCAACCAGTTTTCCAATTCAAGTTTTACTGCTTCTGGTTTTTCATGGGGAGCAATTCCGCATCTCCCTTCTATGACGACACGATCTGCAACTGACGAAGGCCACGCTCCCCCTGAGATGGTGCCGATATTCACTGGTACCGGTATTGGGACATTGTCATACAGCGGATCTGAAATTCTCG  >  NZ_CP034943/2145667‑2145942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: