BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,154,383	G→A	6.1%	L174L (CTA→TTA)	EO946_RS10855 ←	phytase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,154,383	0	G	A	6.1%	409.3 / 11.5	131	L174L (CTA→TTA)	EO946_RS10855	phytase
Reads supporting (aligned to +/- strand): ref base G (58/64); new base A (4/4); total (62/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01

Only 100 of 132 total aligned reads displayed.

TCCCGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATT  >  NZ_CP034943/2154245‑2154522

tCCCGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGt                                                                                                                                            <  2:1942238/140‑1 (MQ=255)

tCCCGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGt                                                                                                                                            <  2:1445742/140‑1 (MQ=255)

 cccGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTg                                                                                                                                           >  1:1991122/1‑140 (MQ=255)

 cccGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTg                                                                                                                                           >  2:831862/1‑140 (MQ=255)

 cccGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTg                                                                                                                                           >  1:1839831/1‑140 (MQ=255)

 cccGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTg                                                                                                                                           >  1:1807213/1‑140 (MQ=255)

   cGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTCGTGCg                                                                                                                                         >  2:1057389/1‑140 (MQ=255)

   cGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCg                                                                                                                                         >  1:2151390/1‑140 (MQ=255)

   cGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTGTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAAGTTCATACTGCTCAAATTCTGCTTGCTTGCCTGTCACTAGTGCg                                                                                                                                         >  2:1790360/1‑140 (MQ=255)

          atcatcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAATTg                                                                                                                                  >  2:2135927/1‑140 (MQ=255)

          atcatcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATg                                                                                                                                  >  1:1546402/1‑140 (MQ=255)

          atcatcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATg                                                                                                                                  >  2:1162023/1‑140 (MQ=255)

          atcatcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATg                                                                                                                                  >  2:943880/1‑140 (MQ=255)

            catcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCt                                                                                                                                >  2:2129436/1‑140 (MQ=255)

            catcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCt                                                                                                                                >  2:1009886/1‑140 (MQ=255)

            catcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCt                                                                                                                                >  2:1630590/1‑140 (MQ=255)

              tcCGCAACCAGGCCTTCAGTTTGAGAATTCAATTTACATTCACGCACTGTTTTCCCTGGTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTcc                                                                                                                              <  2:502288/140‑1 (MQ=255)

              tcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTcc                                                                                                                              <  2:2428483/140‑1 (MQ=255)

              tcCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGGTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTcc                                                                                                                              <  2:530124/140‑1 (MQ=255)

                      aaGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTGTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCt                                                                                                                      <  2:2197801/140‑1 (MQ=255)

                        ggCCTTCAGTTTGAGAATTCAAGTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTgg                                                                                                                    <  1:2422363/140‑1 (MQ=255)

                         gCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGc                                                                                                                   >  2:1243018/1‑140 (MQ=255)

                          ccTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCt                                                                                                                  <  2:1991122/140‑1 (MQ=255)

                          ccTTCAGTTGGAGAATTCAAGTTCAAGTCACGCACTTTGTTCCCTGTTACATAACCCTTTCCATTATCCGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCt                                                                                                                  <  1:2430505/140‑1 (MQ=255)

                             tCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTATCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTACTGTTTTCTGGCtgtg                                                                                                               >  1:783308/1‑140 (MQ=255)

                                gtgtGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTGTCCCTTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATa                                                                                                            <  1:2182973/137‑1 (MQ=255)

                                gTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATa                                                                                                            <  1:1009886/140‑1 (MQ=255)

                                gTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATa                                                                                                            <  1:1630590/140‑1 (MQ=255)

                                gTTTGAGAAGTCAATTTAAAGTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATa                                                                                                            <  1:813947/140‑1 (MQ=255)

                                       aaTTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTg                                                                                                     <  2:2310623/140‑1 (MQ=255)

                                         ttCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATa                                                                                                            <  1:2111656/131‑1 (MQ=255)

                                         ttCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATa                                                                                                            >  2:2111656/1‑131 (MQ=255)

                                         ttCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATa                                                                                                            <  1:996345/131‑1 (MQ=255)

                                               ttAAATTCACGCACCTTTTTCCCTGTTACATAACCCTTTCCACCATCAACAATTTCATACTGCTCAAATTCCCCTTGTTTGCCTGTCACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGAATCCATa                                                                                             >  1:240209/1‑140 (MQ=255)

                                                                tttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAc                                                                            <  2:2108380/140‑1 (MQ=255)

                                                                tttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAc                                                                            <  2:936377/140‑1 (MQ=255)

                                                                tttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAc                                                                            <  2:937431/140‑1 (MQ=255)

                                                                 ttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACt                                                                           <  1:1187525/140‑1 (MQ=255)

                                                                 ttCCCTGTTACATAACCCTTTCCATTATCAGCAATTGACTACTGCTCAACTTCTCCTGGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACt                                                                           <  1:2324840/140‑1 (MQ=255)

                                                                          aCATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAgg                                                                  <  1:958847/140‑1 (MQ=255)

                                                                          aCATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAgg                                                                  <  1:1037282/140‑1 (MQ=255)

                                                                          aCATAACCCTTTCCACCATCAACAATTTCATACTGCTCAAATTCCCCTTGTTTGCCTGTCACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGAATCCATAAACCTCAGAAATATTGGTGGAAATAgg                                                                  <  2:240209/140‑1 (MQ=255)

                                                                           cagaaCCCTTTCCATGATCAGCAATTTCCTACTGCTCAAAGTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGa                                                                 <  1:695434/137‑1 (MQ=255)

                                                                                cccTTTCCATTATCAGCACTTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGttt                                                            >  2:584024/1‑140 (MQ=255)

                                                                                cccTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGttt                                                            <  2:712686/140‑1 (MQ=255)

                                                                                  cTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCg                                                          >  2:740448/1‑140 (MQ=255)

                                                                                  cTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCg                                                          >  2:1045937/1‑140 (MQ=255)

                                                                                  cTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCg                                                          >  2:2162386/1‑140 (MQ=255)

                                                                                   tttCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                         >  2:680651/1‑140 (MQ=255)

                                                                                   tttCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                         <  1:2216681/140‑1 (MQ=255)

                                                                                   tttCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCGTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                         <  2:506913/140‑1 (MQ=255)

                                                                                   tttCCATTATCAGCAATTTCATACTGCTCAAATTCTCCGTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                         <  1:380767/140‑1 (MQ=255)

                                                                                     tCCATTCTCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                       <  2:712163/140‑1 (MQ=255)

                                                                                     tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGGTCCTGTTTTCTGGGTGTGATAGAGACTGAATCAGTAAAGTTCAGTAATATCAGTGGAAATTGGAGGTTTGGGGt                                                       >  2:2030593/1‑140 (MQ=255)

                                                                                     tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGACATAGGATGTTTCGGGt                                                       >  1:904174/1‑140 (MQ=255)

                                                                                     tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                         >  2:374653/1‑138 (MQ=255)

                                                                                     tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                       >  1:2243444/1‑140 (MQ=255)

                                                                                     tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                       >  1:937756/1‑140 (MQ=255)

                                                                                     tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                       <  1:1243018/140‑1 (MQ=255)

                                                                                     tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                       >  1:1211776/1‑140 (MQ=255)

                                                                                     tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCGGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGCAATATCACTGGAAATCGGAGGTTTAGGGt                                                       >  1:1670554/1‑140 (MQ=255)

                                                                                          tATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTa                                                  >  2:469139/1‑140 (MQ=255)

                                                                                          tATCAGCAATTTCATACTGCTCAAATTCTCCTTGATTGCCTGTAACTAGTGCGTAAAATTCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAATGGAAATAGGATGTTTCGGGTCTGTa                                                  >  1:506913/1‑140 (MQ=255)

                                                                                           aTCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTaa                                                 <  2:1510873/140‑1 (MQ=255)

                                                                                              aGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATAc                                              <  2:1217503/140‑1 (MQ=255)

                                                                                                cAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTaa                                                 >  2:2323197/1‑135 (MQ=255)

                                                                                                cAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTaa                                                 >  2:114518/1‑135 (MQ=255)

                                                                                                   tttCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTc                                         >  1:2390536/1‑140 (MQ=255)

                                                                                                    ttCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCa                                        <  2:1839831/140‑1 (MQ=255)

                                                                                                      aaTAATGCTCAAATTCTCGTTGTTTTCCTGTCACTATTGCGTTTATTGCTCCTGTTTTCTGGCTGTTATACTGTCTGAATCCGTACACTTCAGATATATGACTGGAAATAGGATGTTTCGGGTATGTAATAATTTTCaaa                                      <  2:1783960/139‑1 (MQ=255)

                                                                                                        tACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGTCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGTGTCTGTAATACTTTTCAAAtt                                    <  2:1546402/140‑1 (MQ=255)

                                                                                                        tACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                    <  1:1962026/140‑1 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                         >  1:2058981/1‑114 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                         <  2:2058981/114‑1 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                    <  1:471858/135‑1 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                    >  2:471858/1‑135 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                    <  1:1143157/135‑1 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                    >  2:1143157/1‑135 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                               >  1:1692620/1‑140 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                               >  1:324846/1‑140 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                               >  2:1425196/1‑140 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                               >  1:147142/1‑140 (MQ=255)

                                                                                                             cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                               >  1:1344735/1‑140 (MQ=255)

                                                                                                                 aagtctcCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACGTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATc                           <  1:1817223/137‑1 (MQ=255)

                                                                                                                   ttCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATccc                         <  2:1687635/140‑1 (MQ=255)

                                                                                                                   ttCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATccc                         <  2:259077/140‑1 (MQ=255)

                                                                                                                       ccTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTTATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTAGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTc                     >  2:1884609/1‑140 (MQ=255)

                                                                                                                       ccTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTc                     >  2:2258300/1‑140 (MQ=255)

                                                                                                                           gcttgcCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCAtt                 <  1:2129436/140‑1 (MQ=255)

                                                                                                                                ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTACACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGta            <  2:1063483/140‑1 (MQ=255)

                                                                                                                                ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGta            >  1:944705/1‑140 (MQ=255)

                                                                                                                                ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGta            <  2:1268165/140‑1 (MQ=255)

                                                                                                                                ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGta            <  1:1852177/140‑1 (MQ=255)

                                                                                                                                ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCGTTATCCCCGTCCATTGCGta            >  1:212488/1‑140 (MQ=255)

                                                                                                                                 cTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTGTATCCCCGTCCATTGCGtat           <  2:1976833/140‑1 (MQ=255)

                                                                                                                                     cACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGa                                                                                                    <  2:2266470/47‑1 (MQ=255)

                                                                                                                                     cACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGa                                                                                                    >  1:2261512/1‑47 (MQ=255)

                                                                                                                                     cACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGa                                                                                                    >  1:2266470/1‑47 (MQ=255)

                                                                                                                                     cACTAATGCGTAAAATGCTCCGGTTTTCTGGCTGTGATACAAGCTGa                                                                                                    >  1:1058933/1‑47 (MQ=255)

                                                                                                                                        tAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGTGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGa    <  1:722965/140‑1 (MQ=255)

TCCCGTACTCATCATCCGCAACAAGGCCTTCAGTTTGAGAATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATT  >  NZ_CP034943/2154245‑2154522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`