BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,154,420	A→G	6.3%	S161S (AGT→AGC)	EO946_RS10855 ←	phytase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,154,420	0	A	G	6.3%	405.8 / 11.8	129	S161S (AGT→AGC)	EO946_RS10855	phytase
Reads supporting (aligned to +/- strand): ref base A (60/60); new base G (4/4); total (64/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 130 total aligned reads displayed.

AATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGACCGATTGGAGGCGGCAGCAA  >  NZ_CP034943/2154284‑2154559

aaTTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTg                                                                                                                                          <  2:2310623/140‑1 (MQ=255)

        ttAAATTCACGCACCTTTTTCCCTGTTACATAACCCTTTCCACCATCAACAATTTCATACTGCTCAAATTCCCCTTGTTTGCCTGTCACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGAATCCATa                                                                                                                                  >  1:240209/1‑140 (MQ=255)

                         tttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAc                                                                                                                 <  2:937431/140‑1 (MQ=255)

                         tttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAc                                                                                                                 <  2:936377/140‑1 (MQ=255)

                         tttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAc                                                                                                                 <  2:2108380/140‑1 (MQ=255)

                         tttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAc                                                                                                                 <  2:496854/140‑1 (MQ=255)

                          ttCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACt                                                                                                                <  1:1187525/140‑1 (MQ=255)

                          ttCCCTGTTACATAACCCTTTCCATTATCAGCAATTGACTACTGCTCAACTTCTCCTGGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACt                                                                                                                <  1:2324840/140‑1 (MQ=255)

                          ttCCCTGTTACATAACCCTGTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACt                                                                                                                <  1:999977/140‑1 (MQ=255)

                                ggtACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAACATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAAt                                                                                                          >  2:2132567/3‑140 (MQ=255)

                                gTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGATCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAAt                                                                                                          >  1:1322205/1‑140 (MQ=255)

                                   aCATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAgg                                                                                                       <  1:1037282/140‑1 (MQ=255)

                                   aCATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAgg                                                                                                       <  1:958847/140‑1 (MQ=255)

                                   aCATAACCCTTTCCACCATCAACAATTTCATACTGCTCAAATTCCCCTTGTTTGCCTGTCACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGAATCCATAAACCTCAGAAATATTGGTGGAAATAgg                                                                                                       <  2:240209/140‑1 (MQ=255)

                                    cagaaCCCTTTCCATGATCAGCAATTTCCTACTGCTCAAAGTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGa                                                                                                      <  1:695434/137‑1 (MQ=255)

                                         cccTTTCCATTATCAGCACTTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGttt                                                                                                 >  2:584024/1‑140 (MQ=255)

                                         cccTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGttt                                                                                                 <  2:712629/140‑1 (MQ=255)

                                         cccTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGttt                                                                                                 <  2:1181710/140‑1 (MQ=255)

                                           cTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCg                                                                                               >  2:2162386/1‑140 (MQ=255)

                                           cTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCg                                                                                               >  2:740448/1‑140 (MQ=255)

                                           cTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCg                                                                                               >  2:1045937/1‑140 (MQ=255)

                                            tttCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                                                              >  2:680651/1‑140 (MQ=255)

                                              tCCATTCTCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                                                            <  2:712163/140‑1 (MQ=255)

                                              tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGGTCCTGTTTTCTGGGTGTGATAGAGACTGAATCAGTAAAGTTCAGTAATATCAGTGGAAATTGGAGGTTTGGGGt                                                                                            >  2:2030593/1‑140 (MQ=255)

                                              tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGACATAGGATGTTTCGGGt                                                                                            >  1:904174/1‑140 (MQ=255)

                                              tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                                                              <  1:374653/138‑1 (MQ=255)

                                              tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                                                            >  1:2243444/1‑140 (MQ=255)

                                              tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                                                            >  1:1842957/1‑140 (MQ=255)

                                              tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                                                            >  1:1211776/1‑140 (MQ=255)

                                              tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGt                                                                                            >  1:937756/1‑140 (MQ=255)

                                              tCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCGGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGCAATATCACTGGAAATCGGAGGTTTAGGGt                                                                                            >  1:1670554/1‑140 (MQ=255)

                                                   tATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTa                                                                                       >  2:469139/1‑140 (MQ=255)

                                                   tATCAGCAATTTCATACTGCTCAAATTCTCCTTGATTGCCTGTAACTAGTGCGTAAAATTCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAATGGAAATAGGATGTTTCGGGTCTGTa                                                                                       >  1:506913/1‑140 (MQ=255)

                                                    aTCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTaa                                                                                      <  2:1510873/140‑1 (MQ=255)

                                                       aGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATAc                                                                                   <  2:1217503/140‑1 (MQ=255)

                                                         cAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTaa                                                                                      >  2:114518/1‑135 (MQ=255)

                                                         cAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTaa                                                                                      <  1:2323197/135‑1 (MQ=255)

                                                             ttCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCa                                                                             <  2:1839831/140‑1 (MQ=255)

                                                                 tACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGTCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGTGTCTGTAATACTTTTCAAAtt                                                                         <  2:1546402/140‑1 (MQ=255)

                                                                 tACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                                                         <  1:1962026/140‑1 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCgg                                                                                              <  2:2058981/114‑1 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAATGTCCCtt                                                                    >  1:2421316/1‑140 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                                                         <  1:471858/135‑1 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                                                         <  1:1143157/135‑1 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAAtt                                                                         >  2:471858/1‑135 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                                                                    >  1:1344735/1‑140 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                                                                    >  2:1425196/1‑140 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                                                                    >  1:1692620/1‑140 (MQ=255)

                                                                      cTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCtt                                                                    >  1:324846/1‑140 (MQ=255)

                                                                        cAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTa                                                                  <  2:274964/140‑1 (MQ=255)

                                                                          aagtctcCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACGTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATc                                                                <  1:1817223/137‑1 (MQ=255)

                                                                          aaTTCTGCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTGCCTTTATc                                                                <  1:1574488/140‑1 (MQ=255)

                                                                          aaTTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATc                                                                <  1:810256/140‑1 (MQ=255)

                                                                            ttCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATccc                                                              <  2:259077/140‑1 (MQ=255)

                                                                            ttCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATccc                                                              <  2:1687635/140‑1 (MQ=255)

                                                                                    gcttgcCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCAtt                                                      <  1:2129436/140‑1 (MQ=255)

                                                                                         ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTACACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGta                                                 <  2:1063483/140‑1 (MQ=255)

                                                                                         ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGta                                                 >  1:944705/1‑140 (MQ=255)

                                                                                         ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGta                                                 <  2:1268165/140‑1 (MQ=255)

                                                                                         ccTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCGTTATCCCCGTCCATTGCGta                                                 >  1:212488/1‑140 (MQ=255)

                                                                                          cTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTGTATCCCCGTCCATTGCGtat                                                <  2:1976833/140‑1 (MQ=255)

                                                                                              cACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGa                                                                                                                                         <  2:2261512/47‑1 (MQ=255)

                                                                                              cACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGa                                                                                                                                         >  1:2261512/1‑47 (MQ=255)

                                                                                              cACTAATGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAAGCTGa                                                                                                                                         <  2:2266470/47‑1 (MQ=255)

                                                                                              cACTAATGCGTAAAATGCTCCGGTTTTCTGGCTGTGATACAAGCTGa                                                                                                                                         >  1:1058933/1‑47 (MQ=255)

                                                                                              cACTAATGCGTAAAATGCTCCCGTTTTCTGGCTGTGATACAAGCTGa                                                                                                                                         <  2:1058933/47‑1 (MQ=255)

                                                                                                 tAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGTGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGa                                         <  1:722965/140‑1 (MQ=255)

                                                                                                 tAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATGCCCTTTATCCCCGTCCATTGCGTATACTTCGa                                         <  1:2016906/140‑1 (MQ=255)

                                                                                                    tGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATc                                                                <  2:523443/114‑1 (MQ=255)

                                                                                                    tGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATc                                                                >  1:2342567/1‑114 (MQ=255)

                                                                                                    tGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATc                                                                >  1:523443/1‑114 (MQ=255)

                                                                                                    tGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATc                                                                <  2:2342567/114‑1 (MQ=255)

                                                                                                    tGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTg                                      >  1:1720067/1‑140 (MQ=255)

                                                                                                    tGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTg                                      >  1:1212720/1‑140 (MQ=255)

                                                                                                    tGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTg                                      >  1:2174268/1‑140 (MQ=255)

                                                                                                      cGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAATTTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTa                                    >  1:1111386/1‑140 (MQ=255)

                                                                                                      cGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTa                                    >  1:1575459/1‑140 (MQ=255)

                                                                                                      cGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTa                                    >  1:834078/1‑140 (MQ=255)

                                                                                                      cGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTa                                    >  1:1930287/1‑140 (MQ=255)

                                                                                                      cGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTa                                    >  1:634676/1‑140 (MQ=255)

                                                                                                        tAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTAtt                                  >  2:753326/1‑140 (MQ=255)

                                                                                                           aaTGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCAGTGGAAATAGGATGTTTCGGGGCTGGAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATGCtt                               >  2:1387399/1‑140 (MQ=255)

                                                                                                           aaTGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCtt                               >  2:665601/1‑140 (MQ=255)

                                                                                                           aaTGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGAAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCATTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCtt                               >  2:1360789/1‑140 (MQ=255)

                                                                                                                tCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCtt                          <  1:1268479/140‑1 (MQ=255)

                                                                                                                tCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCtt                          <  2:1842957/140‑1 (MQ=255)

                                                                                                                tCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCtt                          <  2:532677/140‑1 (MQ=255)

                                                                                                                tCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCtt                          <  2:937756/140‑1 (MQ=255)

                                                                                                                tCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCtt                          <  1:2063955/140‑1 (MQ=255)

                                                                                                                tCCTGTTTTCGGGCTGTGATACAGACTGAATCCGTAAACTGCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCAGTGCGTATACTTCGATTGTATTCTTTCCtt                          <  2:1877385/140‑1 (MQ=255)

                                                                                                                    gTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGa                      <  2:289988/140‑1 (MQ=255)

                                                                                                                    gTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGa                      <  2:1737007/140‑1 (MQ=255)

                                                                                                                     ttttCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGCTGTTTCGGGTCTGTAATACTTTTCACATTCCCTTTATCCCCGTCCGTTGCGTATACTTCGATTGTTGTCTTTCCGGCAGAc                     >  2:2354892/1‑140 (MQ=255)

                                                                                                                     ttttCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGAc                     >  2:1520433/1‑140 (MQ=255)

                                                                                                                          tGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGACCGAtt                >  2:2333594/1‑140 (MQ=255)

                                                                                                                          tGGCTGTCATACAGACTTAATCCGTAAACATCAGAAATATCACTGGAAATAGGATGTTTAGGGTATGTAATACATTTCAAAGTCCCTTTATCCCCGACCATTCCGTATACTTAGAATGAATTCTTTCCTTCATACCTAtt                >  2:2200171/1‑140 (MQ=255)

                                                                                                                               gtgATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGACCGATTGGAgg           >  2:1342960/1‑140 (MQ=255)

                                                                                                                               gtgATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGACCGATTGGAgg           >  2:1342971/1‑140 (MQ=255)

                                                                                                                                tgATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGACCGATTGGAggc          <  1:2258300/140‑1 (MQ=255)

                                                                                                                                      aGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGACCGATTGGAGGCGgcagc    >  2:848828/1‑140 (MQ=255)

AATTCAATTTAAATTCACGCACTTTTTTCCCTGTTACATAACCCTTTCCATTATCAGCAATTTCATACTGCTCAAATTCTCCTTGCTTGCCTGTCACTAGTGCGTAAAATGCTCCTGTTTTCTGGCTGTGATACAGACTGAATCCGTAAACTTCAGAAATATCACTGGAAATAGGATGTTTCGGGTCTGTAATACTTTTCAAATTCCCTTTATCCCCGTCCATTGCGTATACTTCGATTGTATTCTTTCCTTCAGACCGATTGGAGGCGGCAGCAA  >  NZ_CP034943/2154284‑2154559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`