BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	417,267	G→T	5.3%	F56L (TTC→TTA)	EO946_RS02145 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	417,267	0	G	T	5.3%	339.5 / 5.4	119	F56L (TTC→TTA)	EO946_RS02145	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (53/58); new base T (4/4); total (57/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TCGCATCTTCAAAGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATCAGT  >  NZ_CP034943/417131‑417405

tCGCATCTTCAAAGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCGTCAGGGGTTCTTTTTTTTCGATTTATAGATAAAGTACATTTCTATCACTAGTATGaaa                                                                                                                                         >  2:253245/1‑140 (MQ=255)

           aaGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATaa                                                                                                                              <  2:605034/140‑1 (MQ=255)

           aaGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATaa                                                                                                                              <  2:1133297/140‑1 (MQ=255)

               aaCCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGTGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGc                                                                                                                          <  2:1348699/140‑1 (MQ=255)

               aaCCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGc                                                                                                                          <  2:909001/140‑1 (MQ=255)

               aaCCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGc                                                                                                                          <  2:418723/140‑1 (MQ=255)

               aaCCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGc                                                                                                                          <  2:2250426/140‑1 (MQ=255)

                 ccATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGAGCACCCCATAACCGGTACTAATATTTAGAATTATAAAAAAAGTAAATTAATATAAAAAATATGAAAAAAAAAAATaaaacaaa                                                                                                                        >  2:1264130/1‑136 (MQ=255)

                 caaTCAACAGACATCCCAGCTATACCATAGACGGAAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCAAGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCAc                                                                                                                        <  2:746365/138‑1 (MQ=255)

                    tCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                                     <  1:788335/140‑1 (MQ=255)

                    tCAACAGACATAGCAGCGATACAATAGACGGAAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                                     <  1:640907/140‑1 (MQ=255)

                    tCAACAGACATAGCAGCGATACAATAGACGGAAATATTATCGATTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                                     <  1:83589/140‑1 (MQ=255)

                       aCAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGTTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCa                                                                                                                  <  2:650020/140‑1 (MQ=255)

                       aCAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCa                                                                                                                  <  2:1647518/140‑1 (MQ=255)

                        caaacacAGCAGTGATACAATAGACGGCAATATGATTGTCTGAAAAAAGCGCCCCCCTTTAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAAGCACGACCCAt                                                                                                                 >  1:2132473/4‑140 (MQ=255)

                        caaacacAGCAGTGATACAATAGACGGCAATATGATTGTCTGAAAAAAGCGCCCCCCTTTAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAAGCACGACCCAt                                                                                                                 >  1:1782591/4‑140 (MQ=255)

                           aCATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCt                                                                                                              >  1:1123439/1‑140 (MQ=255)

                               agcagcGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCaa                                                                                                          >  1:1530778/1‑140 (MQ=255)

                                     gATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                                     >  1:1198427/1‑123 (MQ=255)

                                     gATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGAc                                                                                                                     <  2:1198427/123‑1 (MQ=255)

                                      aTACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAACAATAAAAGCACGACCCCTGCTGCAAAcggcgt                                                                                                   >  1:1728080/1‑140 (MQ=255)

                                      aTACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAAcgtcgt                                                                                                   >  1:1652162/1‑140 (MQ=255)

                                      aTACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAAcgtcgt                                                                                                   <  2:1469517/140‑1 (MQ=255)

                                      aTACAATAGACCGAAATATTATCGCTTGAAAAAAGCCCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAACCACGACCCATTCTGCAAAcgtcgt                                                                                                   <  1:1365505/140‑1 (MQ=255)

                                         cAATAGACGGCACTATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCAt                                                                                                >  1:1802236/1‑140 (MQ=255)

                                           aTAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCt                                                                                              <  2:268888/140‑1 (MQ=255)

                                           aTAGACGGCAATATTATCGATTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCt                                                                                              <  1:2142580/140‑1 (MQ=255)

                                           aTAGACGGAAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCt                                                                                              <  1:315176/140‑1 (MQ=255)

                                              gACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTATTATGAAAACATAAAATAAAATCACGACCCATTATGCAAACGTCGTCATCTTaa                                                                                           >  1:366454/1‑140 (MQ=255)

                                                 ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTCTAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCCTTCTGCAAACGTCGTCATCTTAAAGc                                                                                        >  1:1257629/1‑140 (MQ=255)

                                                 ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATATATAACGTACATTTCTATCAATAGTATTAAAACAAAAAATAAAAAAACGACACATTATTAAAAAGAAGTAATCTTAAAga                                                                                        >  2:2121351/1‑139 (MQ=255)

                                                 ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                        >  1:1189230/1‑140 (MQ=255)

                                                 ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                        >  1:1460797/1‑140 (MQ=255)

                                                 ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                        >  1:1133411/1‑140 (MQ=255)

                                                 ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                        <  1:601902/140‑1 (MQ=255)

                                                 ggCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                        >  1:526042/1‑140 (MQ=255)

                                                  gCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTCATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCCCGACCCATTCTGCAAACGTCGTCATCTTAAAGCt                                                                                       <  2:521620/140‑1 (MQ=255)

                                                   cAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCTATTTATATATAACGTAAATTTCTCTAAAAAGTATGAAAACAAAAAATAAAAGCAAGACCCATTCTGCAAAAGTAGTCATCTTAAAGCTg                                                                                      >  2:601902/1‑140 (MQ=255)

                                                   cAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTAGATATATAGATAAAGTACATTTCTCTCAAAAGAATGAAAACAAAAAAAAAAAGAACGAAACAATATGAAAAAGTCGTAATCATAAAGATg                                                                                      >  2:1005625/1‑140 (MQ=255)

                                                      tattatCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGAc                                                                                   >  1:2249675/1‑140 (MQ=255)

                                                         tatCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGCTCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAACGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACata                                                                                <  2:473562/140‑1 (MQ=255)

                                                         tatCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACata                                                                                <  2:2116544/140‑1 (MQ=255)

                                                         tatCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACata                                                                                <  1:2120089/140‑1 (MQ=255)

                                                         tatCGATTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACata                                                                                <  1:1510337/140‑1 (MQ=255)

                                                           tCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTATCTCACTAGTATGAAAACAAAAAATAAAAGCACGAACCATTATGCAAACTTAGTCATATTAAAGCTGGACataat                                                                              >  2:191365/1‑138 (MQ=255)

                                                           tCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATAAAGAAAAATTCTCTCACTAGTATGAAAACAAAAAATAAAAGAACGACCCATTCTGAAAACATCGTAATCTTAAAGAAGGAAATAtt                                                                              >  2:1984781/1‑140 (MQ=255)

                                                           tCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTATATTTATATATAAAGTACAATACAATCACTAATATGAAAACAAAAAATAAAATAAAGACCAATTCTGAAAAAGTCGTCATCTTAAAGCTGGACAAAtt                                                                              >  2:2379541/1‑140 (MQ=255)

                                                                tGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCTATATATAGATCACGTAAATTTATATAACTAGTATTAAAACAAAAAATAAAAGAAAGACACATACTGCAAACGACTACATCTTAAAGATTAACataaagatat                                                                         >  2:2405662/1‑133 (MQ=255)

                                                                tGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTtat                                                                         >  1:118062/1‑140 (MQ=255)

                                                                tGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTATCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTtat                                                                         >  1:1658809/1‑140 (MQ=255)

                                                                             cctcTTCAGGTGTTATTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATTAAAAAAAAAAATAAAAGCACTACAAATTCTTCAAACGTAGTCATATTAAAGATGGAAATATTGTTATAATTGGCtttttt                                                            <  2:724008/137‑3 (MQ=255)

                                                                             ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                            <  1:910148/140‑1 (MQ=255)

                                                                             ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                            <  1:1731053/140‑1 (MQ=255)

                                                                             ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                            <  2:448942/140‑1 (MQ=255)

                                                                             ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                            <  1:149506/140‑1 (MQ=255)

                                                                             ccGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                            <  2:2363632/140‑1 (MQ=255)

                                                                             ccGCTTCAGGGGTTCTATTTTTTCGATTTATAGATCACGTACCTTTCTCTCACTAGTATGAAAACAAAAAATAAAACCACGACCCAGTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGt                                                            <  2:1552617/140‑1 (MQ=255)

                                                                               gCTTCAGGGTTTATTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCa                                                          <  1:68048/140‑1 (MQ=255)

                                                                               gCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCa                                                          <  1:2039495/140‑1 (MQ=255)

                                                                                 ttCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGc                                                                                        <  1:701188/108‑1 (MQ=255)

                                                                                     ggggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGa                                                    <  1:1910492/140‑1 (MQ=255)

                                                                                     ggggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGa                                                    <  1:1321627/140‑1 (MQ=255)

                                                                                     ggggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGa                                                    <  1:318984/140‑1 (MQ=255)

                                                                                      gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGaa                                                   <  2:1034466/140‑1 (MQ=255)

                                                                                      gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGaa                                                   <  2:2312965/140‑1 (MQ=255)

                                                                                      gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGaa                                                   <  2:2091230/140‑1 (MQ=255)

                                                                                      gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGaa                                                   <  2:1825645/140‑1 (MQ=255)

                                                                                      gggTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTCTCAAACTGaa                                                   <  1:1162163/140‑1 (MQ=255)

                                                                                          tCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAACACAAAATATAAAAGCACGACCCATTCTGCAAACGTCGTCCTCTTAAAGCTGGAGATATTGTTATAAATGGCTTGTGTCAAGCTGcaagcg                                               >  2:1311248/1‑137 (MQ=255)

                                                                                          tCTTTATTTTCGATTTATAGATCACGTACATTTCTCTCCCTAGTATGAAAACAAAAAATAAAAGCACGACCCATACTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTCAAAccg                                               >  2:635206/1‑139 (MQ=255)

                                                                                           cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                              >  1:1631215/1‑140 (MQ=255)

                                                                                           cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                              >  1:1886795/1‑140 (MQ=255)

                                                                                           cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                              >  2:2314380/1‑140 (MQ=255)

                                                                                           cTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCtt                                              >  2:2316642/1‑140 (MQ=255)

                                                                                                      aTTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCAGTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTcc                                   >  2:459487/1‑140 (MQ=255)

                                                                                                        ttATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAATCACGACCCATTCCGCAAATGTTGTCATCTTAAAGCTGGACATATTGTTATAAACGGATTTTGTTAAACTGAACCCTTGAAGCATGTCCAt                                 <  1:1237929/140‑1 (MQ=255)

                                                                                                        ttATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAAGCACGACCCATTCCGCAAATGTTGTCATCTTAAAGCTGGACATATTGTTATAAACGGCTTTTGTTAAACTGAACCCTTGAAGCATGTCCAt                                 <  2:1782591/140‑1 (MQ=255)

                                                                                                        ttATAGATCACGTACATTTCTCTCACAAGTACTAACACAAAAAATAAAAGCACGACCCATTCCGCAAATGTTGTCATCTTAAAGCTGGACATATTGTTATAAACGGCTTTTGTTAAACTGAACCCTTGAAGCATGTCCAt                                 <  2:2132473/140‑1 (MQ=255)

                                                                                                          ataGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATaa                               >  1:1479564/1‑140 (MQ=255)

                                                                                                                   gTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTCTAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATTTCCATAAAAACAGaaa                      >  2:1216621/1‑140 (MQ=255)

                                                                                                                             ctcACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTtaataa            >  2:2053395/1‑140 (MQ=255)

                                                                                                                             ctcACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTtaataa            >  2:2334282/1‑140 (MQ=255)

                                                                                                                             ctcACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTtaataa            >  2:772791/1‑140 (MQ=255)

                                                                                                                             ctcACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTtaataa            >  2:852309/1‑140 (MQ=255)

                                                                                                                                aCTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACa         >  1:1699440/1‑140 (MQ=255)

                                                                                                                                aCTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACa         >  1:81380/1‑140 (MQ=255)

                                                                                                                                aCTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACa         >  1:416614/1‑140 (MQ=255)

                                                                                                                                 cTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACCTATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACaa        >  2:679831/1‑140 (MQ=255)

                                                                                                                                   aGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAAt      <  1:986417/140‑1 (MQ=255)

                                                                                                                                   aGTATGAAAACAAAAAATAAAAGCAATACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCCTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAAt      <  2:2011862/140‑1 (MQ=255)

                                                                                                                                    gTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATc     <  1:2383336/140‑1 (MQ=255)

                                                                                                                                    gTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATc     <  1:1852843/140‑1 (MQ=255)

                                                                                                                                    gTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATc     <  1:2385962/140‑1 (MQ=255)

                                                                                                                                     tATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATCa    >  1:1096966/1‑140 (MQ=255)

                                                                                                                                     tATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATCa    >  1:2088350/1‑140 (MQ=255)

                                                                                                                                     tATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATCa    >  2:1713712/1‑140 (MQ=255)

                                                                                                                                     tATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATCa    >  1:420649/1‑140 (MQ=255)

                                                                                                                                     tATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACACATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATCa    >  2:291323/1‑140 (MQ=255)

                                                                                                                                       tGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATCAGt  <  2:1479564/140‑1 (MQ=255)

                                                                                                                                       tGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAAAGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATTTCCATAAAAACAGAAATGGTTAATAAACAAATCAGt  <  2:1643369/140‑1 (MQ=255)

TCGCATCTTCAAAGGAACCATCAACAGACATAGCAGCGATACAATAGACGGCAATATTATCGCTTGAAAAAAGCGCCCCGCTTCAGGGGTTCTTTTTTTTCGATTTATAGATCACGTACATTTCTCTCACTAGTATGAAAACAAAAAATAAAAGCACGACCCATTCTGCAAACGTCGTCATCTTAAAGCTGGACATATTGTTATAAATGGCTTTTGTCAAACTGAAACCTTGTAGCATGTCCATAAAAACAGAAATGGTTAATAAACAAATCAGT  >  NZ_CP034943/417131‑417405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`