Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,165,568 T→G 5.1% E94D (GAA→GAC EO946_RS10925 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,165,5680TG 5.1% 299.0 / 2.8 129E94D (GAA→GACEO946_RS10925hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (44/75);  new base G (3/7);  total (47/82)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TGTCCTTTTTCCAGCTGTTTCGTCACATCTTCATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGATGCCGATACAGCCTTCCAAAA  >  NZ_CP034943/2165431‑2165707
                                                                                                                                         |                                                                                                                                           
tctacTTTTTCCAGATGTTTCGTCACATCTTCATATAACTATTAATAATCGCCGTATTAACTCACATCCAACAGAACTATCAAATGCCAGATAAAACGCAAACGAAATACTATGGGAATCGCAATAAAACCTGCCAGtta                                                                                                                                           >  2:1256436/5‑139 (MQ=255)
tGTCCTTTTTCCAGCTGTTTCGTCACATCTTCATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTc                                                                                                                                           <  2:1336519/140‑1 (MQ=255)
 gTCATTTTTCCAGGTGTTTCGTCACATCTTCATATACCTTTTCTTAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCTATAAAAGGCAAGCTAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTcc                                                                                                                                          <  1:723101/140‑1 (MQ=255)
             gCTGTTTCGTCACATCTTCATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCTAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCa                                                                                                                              <  2:731673/140‑1 (MQ=255)
             gCTGTTTCGTCACATCTTCATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCa                                                                                                                              >  1:2211694/1‑140 (MQ=255)
                 tttCGTCACATCTTCATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCaa                                                                                                                          >  2:914075/1‑140 (MQ=255)
                 tttCGTCACATCTTCATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCaa                                                                                                                          >  2:1668907/1‑140 (MQ=255)
                 tttCGTCACATCTTCATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCaa                                                                                                                          >  2:1115182/1‑140 (MQ=255)
                               cATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCa                                                                                                            >  1:1840370/1‑140 (MQ=255)
                                      cTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCcaaac                                                                                                     >  1:1108581/1‑140 (MQ=255)
                                        tttCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCcaaacgc                                                                                                   >  1:2295011/1‑140 (MQ=255)
                                         ttCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCcaaacgca                                                                                                  >  1:1175345/1‑140 (MQ=255)
                                         ttCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCcaaacgca                                                                                                  >  1:1267799/1‑140 (MQ=255)
                                         ttCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCcaaacgca                                                                                                  >  1:1649761/1‑140 (MQ=255)
                                         ttCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTGTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCcaaacgca                                                                                                  >  2:1420988/1‑140 (MQ=255)
                                         tgcTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCcaaacgca                                                                                                  >  1:626751/3‑140 (MQ=255)
                                              aaTCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGGTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACga                                                                                             >  2:462559/1‑140 (MQ=255)
                                              aaTCGCCGTCGGAAATCACATCCATGCGATCTAGCGATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCCATAAAACCGGCCGGGTCCTCAATCTATCCCGCCACTAAGGCCAGTTCTGCcaaacgcgcacgg                                                                                             >  2:553556/1‑134 (MQ=255)
                                                    cGTCTGAACTCACATCCATCCGATCGAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGc                                                                                       <  2:1987185/140‑1 (MQ=255)
                                                    cGTCTGAAATCACATCCTTCCGATCGAGCCATGGCCCGATAAGAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCTATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGc                                                                                       <  2:1500121/140‑1 (MQ=255)
                                                    cGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCTAAATACTTTGGGATTGGAATTAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCGAGTTCAGCCAACCGCAAACGATACTGc                                                                                       <  2:1886150/140‑1 (MQ=255)
                                                    cGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGc                                                                                       <  2:1425215/140‑1 (MQ=255)
                                                    cGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGc                                                                                       <  1:907017/140‑1 (MQ=255)
                                                    cGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGc                                                                                       <  1:81092/140‑1 (MQ=255)
                                                    cGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGGTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGc                                                                                       <  1:560244/140‑1 (MQ=255)
                                                    cGTCTGAAATCACATCCATCCGATATAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTTGCAATAAAACCGGCCGGTTCCTCAATATCTCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGc                                                                                       <  2:1886125/140‑1 (MQ=255)
                                                         gAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACata                                                                                  <  1:75809/140‑1 (MQ=255)
                                                         gAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACata                                                                                  <  1:540490/140‑1 (MQ=255)
                                                         gAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGAAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACata                                                                                  <  1:699020/140‑1 (MQ=255)
                                                         gAAATCACATCCATCCGATCTAGCCATTGCCCGAAAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACata                                                                                  <  1:331972/140‑1 (MQ=255)
                                                                    catccGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCACACGAGACTGCAGATATAAAACAGGAc                                                                       >  1:2167550/1‑140 (MQ=255)
                                                                    catccGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGAc                                                                       >  1:1039551/1‑140 (MQ=255)
                                                                      tccGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcg                                                                     <  1:2261267/140‑1 (MQ=255)
                                                                        ctaTCTAGCCATGGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCCGCACCTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAACCAGGACcgcg                                                                   <  1:1795020/138‑1 (MQ=255)
                                                                        cGATCTAGCTATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  2:1397755/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCTAAATACTTTGGGATTGGAAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:751947/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCGCATATAAAACAGGACcgcg                                                                   <  1:2075255/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:624940/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:604273/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:1093795/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:531768/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:1622193/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:372000/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:1759174/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:1985236/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:1387263/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:1405482/140‑1 (MQ=255)
                                                                        cGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGGTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:523097/140‑1 (MQ=255)
                                                                         taTCTAGCCATTGCCAGATAAAAGGAAAGCTAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  <  2:606329/139‑1 (MQ=255)
                                                                         gATCTCGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  <  2:846319/140‑1 (MQ=255)
                                                                         gATCTAGCCATTGCCCGATACAAGGCAAGCGAACTACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCCGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  <  1:249732/140‑1 (MQ=255)
                                                                         gATCTAGCCATTGCCCGATAAAAGGCAAGCTAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  <  2:634758/140‑1 (MQ=255)
                                                                         gATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  <  2:1066831/140‑1 (MQ=255)
                                                                         gATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGAAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  <  1:1438386/140‑1 (MQ=255)
                                                                         gATCTAGCCATTGCACGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACggcgc                                                                  <  1:2069549/140‑1 (MQ=255)
                                                                         gATCTAGCCATTGACCTATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  <  2:1783440/140‑1 (MQ=255)
                                                                         gATCTAGCCAGTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAACACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  <  1:2069681/140‑1 (MQ=255)
                                                                             tAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTa                                                              <  1:1890963/140‑1 (MQ=255)
                                                                                    tGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCCCATATAAAACAGGACCGCGCGGTATTGGTTc                                                       >  1:1569905/1‑140 (MQ=255)
                                                                                    tGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACata                                                                                  >  1:2202998/1‑113 (MQ=255)
                                                                                    tGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGa                                                                        >  2:207856/1‑123 (MQ=255)
                                                                                    tGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGa                                                                        <  1:207856/123‑1 (MQ=255)
                                                                                    tGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTc                                                       >  1:1447714/1‑140 (MQ=255)
                                                                                    tGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAAAATATCAAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGACTGCACATATAAAACTGGACCTCGCGGTATTGGTTc                                                       >  2:185997/1‑140 (MQ=255)
                                                                                     gCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCACTATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCa                                                      >  2:1900382/1‑140 (MQ=255)
                                                                                     gCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCa                                                      >  2:2288074/1‑140 (MQ=255)
                                                                                      cccGATAAAAGGCAAGCGAAATACTTTGGTATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAg                                                     <  1:2340800/140‑1 (MQ=255)
                                                                                      cccGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAg                                                     <  1:478534/140‑1 (MQ=255)
                                                                                       ccGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCACTATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAgg                                                    <  2:696937/140‑1 (MQ=255)
                                                                                          aTAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcgc                                                                  >  2:1551227/1‑123 (MQ=255)
                                                                                          aTAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTg                                                 >  1:2286478/1‑140 (MQ=255)
                                                                                              aatgCAATCGAAATACTTTGGGATTTGCAATAAAACCGGCGGGTTACTCAATATATCCAGCAACTAAGGCCAGTTGAGCCAAAAGCAAACGAGCATGCCCATATAAAACAGGACCGGGCGGTATTGGTTCAGGCTGATgg                                             <  1:142668/137‑1 (MQ=255)
                                                                                              aaGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATgg                                             <  1:1644772/140‑1 (MQ=255)
                                                                                              aaGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATgg                                             <  1:738435/140‑1 (MQ=255)
                                                                                                ggCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGAt                                           <  2:2397495/140‑1 (MQ=255)
                                                                                                    aGCGAAATACTTTGGGTTTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTc                                       <  1:479325/140‑1 (MQ=255)
                                                                                                    aGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTc                                       >  2:651169/1‑140 (MQ=255)
                                                                                                    aGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTc                                       >  2:128228/1‑140 (MQ=255)
                                                                                                    aGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTc                                       <  1:1651113/140‑1 (MQ=255)
                                                                                                     gCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATCTATCCAGCAAGTAAGGCCAGTTCAGCCAAACGCCAAGGAGAATGCAAATATAATACAGGACCGCGCGGTAATGGTACAGGCTGATGTATCggcg                                      >  2:2384723/1‑137 (MQ=255)
                                                                                                     gCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTca                                      >  1:685092/1‑140 (MQ=255)
                                                                                                     gCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTca                                      >  1:685110/1‑140 (MQ=255)
                                                                                                        aaaTACTTTGGGATTTGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACaa                                   <  1:1362985/140‑1 (MQ=255)
                                                                                                        aaaTACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTAAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACaa                                   <  2:2425453/140‑1 (MQ=255)
                                                                                                                  ggATTGGCAATACACCCGGCCGGGTCCTCCATATATCCAGCAACGCAGGCCAGTACAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGt                         <  1:2369670/140‑1 (MQ=255)
                                                                                                                  ggATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGt                         <  2:2079067/140‑1 (MQ=255)
                                                                                                                  ggATTGGCAATAAAACCGGCCGGGTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGt                         <  2:2348379/140‑1 (MQ=255)
                                                                                                                   gATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTg                        >  2:652313/1‑140 (MQ=255)
                                                                                                                     ttGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGGATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGAt                      <  2:2233044/140‑1 (MQ=255)
                                                                                                                     ttGGCAATAAAACCGGCCGGGTCCTCAATCTATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAATCGAGAATGCACAAATAAAACAGGACCGCGCCGTATTGGTTCACGCTGATGGATCGTCACAACTGAACCGGTGAt                      <  2:1643354/140‑1 (MQ=255)
                                                                                                                      tGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCAGAAGTGAACCGGTGagg                     >  1:1573055/1‑138 (MQ=255)
                                                                                                                      tGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGATg                     >  1:689619/1‑140 (MQ=255)
                                                                                                                       ggCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGa                                                                        <  2:922626/88‑1 (MQ=255)
                                                                                                                          aaTAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGATGCCGa                 <  1:369001/140‑1 (MQ=255)
                                                                                                                            tAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACcgcg                                                                   <  1:1949697/88‑1 (MQ=255)
                                                                                                                            tAAAACCGGCCGGTTCCGCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGATGCCGATa               >  2:590843/1‑140 (MQ=255)
                                                                                                                            tAAAACCGGCCGGGTCCTCAAAATATCCAGCAACTAAGGCCCGTTCAGCCAAACGCACACGAGAATGCAGCTATACAACAGGACCGCGCGGTATTGGTTCAGGCGGATGGAGCGTCACATCTGAACCGGTGATGCCGAtc               >  2:1049950/1‑139 (MQ=255)
                                                                                                                             aaaaCCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGATGCCGATAc              >  2:1669736/1‑140 (MQ=255)
                                                                                                                                  cggccggTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGATGCCGATACAGCCt         <  1:1214746/140‑1 (MQ=255)
                                                                                                                                  cggccggGTCCTCAATATATCCAGCAACTAAGGCCAGTACAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGATGCCGATACAGCCt         <  1:1214840/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGTCCTTTTTCCAGCTGTTTCGTCACATCTTCATATACCTTTTCTGAATCGCCGTCTGAAATCACATCCATCCGATCTAGCCATTGCCCGATAAAAGGCAAGCGAAATACTTTGGGATTGGCAATAAAACCGGCCGGTTCCTCAATATATCCAGCAACTAAGGCCAGTTCAGCCAAACGCAAACGAGAATGCACATATAAAACAGGACCGCGCGGTATTGGTTCAGGCTGATGGATCGTCACAACTGAACCGGTGATGCCGATACAGCCTTCCAAAA  >  NZ_CP034943/2165431‑2165707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: