Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,180,184 T→C 6.8% D18D (GAT→GAC sspL → small, acid‑soluble spore protein L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,180,1840TC 6.8% 318.3 / 10.2 103D18D (GAT→GACsspLsmall, acid‑soluble spore protein L
Reads supporting (aligned to +/- strand):  ref base T (25/71);  new base C (4/3);  total (29/74)
Fisher's exact test for biased strand distribution p-value = 9.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 106 total aligned reads displayed.

ATCATCTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTG  >  NZ_CP034943/2180047‑2180323
                                                                                                                                         |                                                                                                                                           
atcatcTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCt                                                                                                                                           >  2:1936098/1‑140 (MQ=255)
 tcatcTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTg                                                                                                                                          <  2:19090/140‑1 (MQ=255)
   atcTGGCAAAATAAGCGATTCGGCTGAATGATCACAATGAAATCTCGATAAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATGTGGa                                                                                                                                        <  2:2434211/140‑1 (MQ=255)
   atcTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGa                                                                                                                                        <  2:724462/140‑1 (MQ=255)
   atcTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGa                                                                                                                                        <  2:1987383/140‑1 (MQ=255)
   atcTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGa                                                                                                                                        <  1:1372171/140‑1 (MQ=255)
   atcTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGa                                                                                                                                        <  1:1994658/140‑1 (MQ=255)
   atcTGGAAAAAGAAGCGATTCGGATGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGa                                                                                                                                        <  2:2251147/140‑1 (MQ=255)
    tcTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGaa                                                                                                                                       <  2:424706/140‑1 (MQ=255)
       ggCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGc                                                                                                                                    <  2:679984/140‑1 (MQ=255)
           aaaGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATa                                                                                                                                <  2:95462/140‑1 (MQ=255)
           aaaGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATa                                                                                                                                <  2:569406/140‑1 (MQ=255)
           aaaGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATa                                                                                                                                <  1:824814/140‑1 (MQ=255)
               aagCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGACGGCTGACCGGCGGTGTTACTCCGCAATGCGATCTGGAAGGCAATAcaca                                                                                                                            >  1:1016812/1‑140 (MQ=255)
                   taTTCGGCTGAATGATAAGACTGAAATCTCGAACAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAATATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  2:1946420/139‑1 (MQ=255)
                   gATTCGGCTTAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  2:754103/140‑1 (MQ=255)
                   gATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  2:445870/140‑1 (MQ=255)
                   gATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  2:164128/140‑1 (MQ=255)
                   gATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  2:1410130/140‑1 (MQ=255)
                   gATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  2:1288195/140‑1 (MQ=255)
                   gATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  1:943677/140‑1 (MQ=255)
                   gATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  1:461470/140‑1 (MQ=255)
                   gATTAGGCTGAATGATAAGAATGAAATCTCTATCAATTGTAATCCTACTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTTTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAAt                                                                                                                        <  2:462599/140‑1 (MQ=255)
                     ttCGGCTTAATGATAAGAATGAAATCTCGATCAATGGACATCCTAATGACGATGTGTACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGa                                                                                                                      <  2:1026991/140‑1 (MQ=255)
                     ttCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGa                                                                                                                      <  1:1113543/140‑1 (MQ=255)
                     ttCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGa                                                                                                                      <  2:1658861/140‑1 (MQ=255)
                     ttCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGa                                                                                                                      <  2:108152/140‑1 (MQ=255)
                     ttCGGCTGAATCATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGa                                                                                                                      <  2:2374723/140‑1 (MQ=255)
                        ggCTGAATGATCAGAATTAAATCTCGATAAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGAccc                                                                                                                   <  2:1589413/140‑1 (MQ=255)
                        ggCTGAATGATAAGACTGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGAccc                                                                                                                   <  1:2370733/140‑1 (MQ=255)
                        ggCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGAccc                                                                                                                   <  2:2254782/140‑1 (MQ=255)
                               tGATAAGAATTAAATCTCGATCAATTTTCATCCTAATGACGAGGTGAAAAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGAAATGCGATCTGGAAGGCAATACACATAATGACACTaaaaaa                                                                                                            >  2:1663686/1‑138 (MQ=255)
                               tGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACa                                                                                                            <  1:1106939/140‑1 (MQ=255)
                               tGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACa                                                                                                            <  1:959174/140‑1 (MQ=255)
                               agATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGCTTAACATACATGAAATAGAAAGATAAAGGCCGGCTGACCGGCGTTGTTACTGCGGAAGGCGATCTGGAAGGCTATACACATTATGACCCTAAAACa                                                                                                            <  2:1122604/139‑1 (MQ=255)
                                gATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGTTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACag                                                                                                           <  1:1187859/140‑1 (MQ=255)
                                gATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACag                                                                                                           <  1:2150869/140‑1 (MQ=255)
                                           aaTCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCaa                                                                                                                                  <  1:2089940/106‑1 (MQ=255)
                                               tagATCAATGGTCATCCTAATGACGATGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAagag                                                                                            <  2:2246706/138‑1 (MQ=255)
                                                    cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                       <  2:1950828/140‑1 (MQ=255)
                                                    cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                       <  1:1773441/140‑1 (MQ=255)
                                                    cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                       <  2:382249/140‑1 (MQ=255)
                                                    cAATGGTCATCATAATGACGAGTTGAACAAACATGAAAAAGAAAGATAAATGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                       <  2:1950837/140‑1 (MQ=255)
                                                       tGGTCATCCTAATGACGATGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGATGGCAATACACATAATGACCCTATAACAGAGCTTGAAGAAAGAGCCaaaaaa                                                                                    <  1:1188285/140‑1 (MQ=255)
                                                         gTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                  <  1:813589/140‑1 (MQ=255)
                                                             tCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAAt                                                                              >  2:617742/1‑140 (MQ=255)
                                                                tAATGACGAGGTGAACAAACATGAAAAAGAAAGATTAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACa                                                                           <  2:650435/140‑1 (MQ=255)
                                                                 aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAACACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                          <  2:583626/140‑1 (MQ=255)
                                                                 aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                          <  2:37935/140‑1 (MQ=255)
                                                                 aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                          <  2:193579/140‑1 (MQ=255)
                                                                 aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                          <  2:1774419/140‑1 (MQ=255)
                                                                 aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                          <  2:1570290/140‑1 (MQ=255)
                                                                 aaTGACGAGGTGAACAAAAATGAAAAAGAAATATAAAGGCCGGCTGACCGTCTGTGTTACTCCTCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGGTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                          <  2:1680433/140‑1 (MQ=255)
                                                                     aCGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACg                                                                      <  1:881276/140‑1 (MQ=255)
                                                                     aCGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACg                                                                      <  1:1599049/140‑1 (MQ=255)
                                                                        aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                   >  1:1391420/1‑140 (MQ=255)
                                                                        aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                   >  1:556730/1‑140 (MQ=255)
                                                                        aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                   >  1:1391497/1‑140 (MQ=255)
                                                                        aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGAccc                                                                                                                   >  2:910315/1‑92 (MQ=255)
                                                                        aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGAccc                                                                                                                   <  1:910315/92‑1 (MQ=255)
                                                                        aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                   >  2:274255/1‑140 (MQ=255)
                                                                        aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                   >  2:1218087/1‑140 (MQ=255)
                                                                        aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                   >  2:281554/1‑140 (MQ=255)
                                                                        aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGAACGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                   >  2:1084762/1‑140 (MQ=255)
                                                                              acaaacaTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGagaag                                                             <  1:1443525/140‑1 (MQ=255)
                                                                                    aTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                  <  2:546010/113‑1 (MQ=255)
                                                                                    aTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                  >  1:546010/1‑113 (MQ=255)
                                                                                     tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                      >  2:223124/1‑140 (MQ=255)
                                                                                     tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                      >  2:1793572/1‑140 (MQ=255)
                                                                                     tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                      >  2:2394731/1‑140 (MQ=255)
                                                                                     tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                      >  1:2028805/1‑140 (MQ=255)
                                                                                     tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaaa                                                      >  2:2394062/1‑138 (MQ=255)
                                                                                      gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTacac                                                     <  1:1628492/140‑1 (MQ=255)
                                                                                      gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTAGAGAGGATCTaaac                                                     <  2:1391420/140‑1 (MQ=255)
                                                                                      gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTAGAGAGGATCTaaac                                                     <  2:556730/140‑1 (MQ=255)
                                                                                         aaagaaagATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAt                                                  <  2:550330/140‑1 (MQ=255)
                                                                                          aagaaagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATCCAAAACGCTAGAGAAGATCTACACGAtc                                                 <  1:894280/140‑1 (MQ=255)
                                                                                              aagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAtctctc                                             >  2:709260/1‑140 (MQ=255)
                                                                                              aagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAtctctc                                             >  2:709686/1‑140 (MQ=255)
                                                                                                     cggCCGGCTGACCGGCGGTGGTACTCCGCAACGCGATCGGGAAGGCAACACACAGAATGACCCTACCCCACCGCGCGAAGAAAGAGCCAAAAAAAGCAATACAACCCGCTAGAGAAGATCTACACGATCTCTCTAGCGtt                                      <  2:2189941/139‑1 (MQ=255)
                                                                                                        ccGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGACGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAACACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAg                                   <  1:1021063/140‑1 (MQ=255)
                                                                                                           gCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCtt                                >  1:1610843/1‑140 (MQ=255)
                                                                                                           gCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCtt                                >  1:1293697/1‑140 (MQ=255)
                                                                                                            cTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAGGCACTACAAAACGCTAGAGAGGATCTAAACGATCTCTCTAGCGTTCAGCttt                               >  2:1082915/1‑140 (MQ=255)
                                                                                                               aCCGGCGGTGGTACTCCTCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAACAAAGCACTACAAAACGCTAGAGAAGATCTACACGATCTCTATAGCGTTCAGCTTTTCa                            <  1:1004471/140‑1 (MQ=255)
                                                                                                                 cggcggTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAg                          >  1:501249/1‑140 (MQ=255)
                                                                                                                     gggggtACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                      <  1:1091786/135‑1 (MQ=255)
                                                                                                                     ggTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                      <  1:2167163/140‑1 (MQ=255)
                                                                                                                     ggTGTTACTCCGCAAGGCGATCTGGAACGCCATAAACATAATGACCCTAACCCAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACCCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                      <  1:966333/140‑1 (MQ=255)
                                                                                                                         ttACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAACACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCt                  <  2:824832/140‑1 (MQ=255)
                                                                                                                           aCTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCa                <  1:1218087/140‑1 (MQ=255)
                                                                                                                            cTCCGCAAGGCGATCGGGAAGGAAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATAAAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCaa               <  2:1440443/140‑1 (MQ=255)
                                                                                                                              ccGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAAtt             >  1:2367401/1‑140 (MQ=255)
                                                                                                                                 cAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCt          >  1:1832268/1‑140 (MQ=255)
                                                                                                                                   aGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCttt        >  2:682346/1‑140 (MQ=255)
                                                                                                                                    ggCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTc                                     >  2:2411824/1‑110 (MQ=255)
                                                                                                                                    ggCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTc                                     <  1:2411824/110‑1 (MQ=255)
                                                                                                                                         tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGACGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg  >  1:438463/1‑140 (MQ=255)
                                                                                                                                         tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg  >  1:452826/1‑140 (MQ=255)
                                                                                                                                         tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGAGCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg  >  1:222148/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ATCATCTGGCAAAAGAAGCGATTCGGCTGAATGATAAGAATGAAATCTCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTG  >  NZ_CP034943/2180047‑2180323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: