Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,180,226 A→G 7.2% E32E (GAA→GAG sspL → small, acid‑soluble spore protein L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,180,2260AG 7.2% 301.3 / 10.0 97E32E (GAA→GAGsspLsmall, acid‑soluble spore protein L
Reads supporting (aligned to +/- strand):  ref base A (51/39);  new base G (4/3);  total (55/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 101 total aligned reads displayed.

TCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGG  >  NZ_CP034943/2180094‑2180363
                                                                                                                                    |                                                                                                                                         
tagATCAATGGTCATCCTAATGACGATGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAagag                                                                                                                                    <  2:2246706/138‑1 (MQ=255)
     cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                                                               <  2:1950828/140‑1 (MQ=255)
     cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                                                               <  1:1773441/140‑1 (MQ=255)
     cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                                                               <  2:382249/140‑1 (MQ=255)
     cAATGGTCATCATAATGACGAGTTGAACAAACATGAAAAAGAAAGATAAATGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                                                               <  2:1950837/140‑1 (MQ=255)
        tGGTCATCCTAATGACGATGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGATGGCAATACACATAATGACCCTATAACAGAGCTTGAAGAAAGAGCCaaaaaa                                                                                                                            <  1:1188285/140‑1 (MQ=255)
          gTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                                                          <  1:813589/140‑1 (MQ=255)
              tCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAAt                                                                                                                      >  2:617742/1‑140 (MQ=255)
                 tAATGACGAGGTGAACAAACATGAAAAAGAAAGATTAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACa                                                                                                                   <  2:650435/140‑1 (MQ=255)
                  aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAACACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                  <  2:583626/140‑1 (MQ=255)
                  aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                  <  2:1774419/140‑1 (MQ=255)
                  aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                  <  2:193579/140‑1 (MQ=255)
                  aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                  <  2:1570290/140‑1 (MQ=255)
                  aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                  <  2:37935/140‑1 (MQ=255)
                  aaTGACGAGGTGAACAAAAATGAAAAAGAAATATAAAGGCCGGCTGACCGTCTGTGTTACTCCTCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGGTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                  <  2:1680433/140‑1 (MQ=255)
                      aCGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACg                                                                                                              <  1:881276/140‑1 (MQ=255)
                      aCGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACg                                                                                                              <  1:1599049/140‑1 (MQ=255)
                         aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                                                           >  1:556730/1‑140 (MQ=255)
                         aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                                                           >  1:1391420/1‑140 (MQ=255)
                         aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                                                           >  1:1391497/1‑140 (MQ=255)
                         aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                                                           >  2:281554/1‑140 (MQ=255)
                         aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                                                           >  2:274255/1‑140 (MQ=255)
                         aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                                                           >  2:1218087/1‑140 (MQ=255)
                         aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGAACGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                                                           >  2:1084762/1‑140 (MQ=255)
                               acaaacaTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGagaag                                                                                                     <  1:1443525/140‑1 (MQ=255)
                                     aTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                                                          >  1:546010/1‑113 (MQ=255)
                                     aTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                                                          <  2:546010/113‑1 (MQ=255)
                                      tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                                                              >  2:223124/1‑140 (MQ=255)
                                      tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                                                              >  1:2028805/1‑140 (MQ=255)
                                      tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                                                              >  2:2394731/1‑140 (MQ=255)
                                      tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                                                              >  2:1793572/1‑140 (MQ=255)
                                      tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaaa                                                                                              >  2:2394062/1‑138 (MQ=255)
                                       gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTacac                                                                                             <  1:1628492/140‑1 (MQ=255)
                                       gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTAGAGAGGATCTaaac                                                                                             <  2:1391497/140‑1 (MQ=255)
                                       gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTAGAGAGGATCTaaac                                                                                             <  2:1391420/140‑1 (MQ=255)
                                       gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTAGAGAGGATCTaaac                                                                                             <  2:556730/140‑1 (MQ=255)
                                          aaagaaagATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAt                                                                                          <  2:550330/140‑1 (MQ=255)
                                          aaagaaagATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAt                                                                                          <  1:617742/140‑1 (MQ=255)
                                           aagaaagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATCCAAAACGCTAGAGAAGATCTACACGAtc                                                                                         <  1:894280/140‑1 (MQ=255)
                                               aagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAtctctc                                                                                     >  2:709260/1‑140 (MQ=255)
                                               aagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAtctctc                                                                                     >  2:709686/1‑140 (MQ=255)
                                                      cggCCGGCTGACCGGCGGTGGTACTCCGCAACGCGATCGGGAAGGCAACACACAGAATGACCCTACCCCACCGCGCGAAGAAAGAGCCAAAAAAAGCAATACAACCCGCTAGAGAAGATCTACACGATCTCTCTAGCGtt                                                                              <  2:2189941/139‑1 (MQ=255)
                                                         ccGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGACGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAACACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAg                                                                           <  1:1021063/140‑1 (MQ=255)
                                                            gCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCtt                                                                        >  1:1610843/1‑140 (MQ=255)
                                                            gCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCtt                                                                        >  1:1293697/1‑140 (MQ=255)
                                                             cTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAGGCACTACAAAACGCTAGAGAGGATCTAAACGATCTCTCTAGCGTTCAGCttt                                                                       >  2:1082915/1‑140 (MQ=255)
                                                                aCCGGCGGTGGTACTCCTCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAACAAAGCACTACAAAACGCTAGAGAAGATCTACACGATCTCTATAGCGTTCAGCTTTTCa                                                                    <  1:1004471/140‑1 (MQ=255)
                                                                  cggcggTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAg                                                                  >  1:501249/1‑140 (MQ=255)
                                                                      gggggtACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              <  1:1091786/135‑1 (MQ=255)
                                                                      ggTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              <  1:2167163/140‑1 (MQ=255)
                                                                      ggTGTTACTCCGCAAGGCGATCTGGAACGCCATAAACATAATGACCCTAACCCAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACCCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              <  1:966333/140‑1 (MQ=255)
                                                                          ttACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAACACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCt                                                          <  2:824832/140‑1 (MQ=255)
                                                                            aCTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCa                                                        <  1:1218087/140‑1 (MQ=255)
                                                                             cTCCGCAAGGCGATCGGGAAGGAAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATAAAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCaa                                                       <  2:1440443/140‑1 (MQ=255)
                                                                               ccGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAAtt                                                     >  1:2367401/1‑140 (MQ=255)
                                                                                  cAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCt                                                  >  1:1832268/1‑140 (MQ=255)
                                                                                    aGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCttt                                                >  2:682346/1‑140 (MQ=255)
                                                                                     ggCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTc                                                                             >  2:2411824/1‑110 (MQ=255)
                                                                                     ggCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTc                                                                             <  1:2411824/110‑1 (MQ=255)
                                                                                          tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGACGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg                                          >  1:438463/1‑140 (MQ=255)
                                                                                          tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg                                          >  1:452826/1‑140 (MQ=255)
                                                                                          tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGAGCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg                                          >  1:222148/1‑140 (MQ=255)
                                                                                            tGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtggt                                        <  1:1462862/140‑1 (MQ=255)
                                                                                                    aaTACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGa                                <  1:1727929/140‑1 (MQ=255)
                                                                                                    aaTACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGa                                >  1:2068410/1‑140 (MQ=255)
                                                                                                    aaTACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGa                                >  1:293670/1‑140 (MQ=255)
                                                                                                    aaTACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGa                                >  1:2337243/1‑140 (MQ=255)
                                                                                                      tACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAg                              >  1:2399558/1‑140 (MQ=255)
                                                                                                             aaTGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCa                       >  2:1775380/1‑140 (MQ=255)
                                                                                                             aaTGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCa                       >  2:1030388/1‑140 (MQ=255)
                                                                                                                  cccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgc                  >  2:1942834/1‑140 (MQ=255)
                                                                                                                  cccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgc                  >  2:1822408/1‑140 (MQ=255)
                                                                                                                  cccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgc                  >  2:1527323/1‑140 (MQ=255)
                                                                                                                   ccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgcc                 >  2:1711122/1‑140 (MQ=255)
                                                                                                                   ccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgcc                 >  1:1316440/1‑140 (MQ=255)
                                                                                                                   ccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgcc                 >  1:474924/1‑140 (MQ=255)
                                                                                                                     tAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATGTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTGCTGTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTg               >  1:1126783/1‑140 (MQ=255)
                                                                                                                     tAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTTGTGGCGGCGAAGCATGTCAGCCGCCTg               >  2:1654769/1‑140 (MQ=255)
                                                                                                                      aaaaCAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCATGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCAGGGCAGCCGCCTGc              >  2:393277/1‑140 (MQ=255)
                                                                                                                        aaCAGAGCTTGAAGAAAGAGCCAAACAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTGTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTc            <  2:274165/140‑1 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              <  2:1496616/85‑1 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              <  2:388777/85‑1 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              >  1:1496616/1‑85 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              >  1:1605202/1‑85 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              >  1:388777/1‑85 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                              <  2:1605202/85‑1 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  2:685812/1‑140 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  2:843217/1‑140 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  2:682231/1‑140 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  1:1966026/1‑140 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  1:2093596/1‑140 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  2:2179898/1‑140 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  2:204408/1‑140 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  2:1773192/1‑140 (MQ=255)
                                                                                                                             agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt       >  2:153343/1‑140 (MQ=255)
                                                                                                                               cTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGTAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGc     <  2:1016812/140‑1 (MQ=255)
                                                                                                                                  gaagaaAGAGCCAAACAAAGCACTACAAAACGCTAGAGAAGAACTACCCGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAgg  <  1:591959/140‑1 (MQ=255)
                                                                                                                                  gaagaaAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAgg  <  1:1463112/140‑1 (MQ=255)
                                                                                                                                  gaagaaAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAgg  <  1:2164029/140‑1 (MQ=255)
                                                                                                                                  gaagaaAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAgg  <  1:682346/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                         
TCGATCAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGG  >  NZ_CP034943/2180094‑2180363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: