Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,180,235 C→A 5.8% A35A (GCC→GCA sspL → small, acid‑soluble spore protein L

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,180,2350CA 5.8% 335.5 / 7.9 111A35A (GCC→GCAsspLsmall, acid‑soluble spore protein L
Reads supporting (aligned to +/- strand):  ref base C (58/46);  new base A (4/3);  total (62/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGAATAAAG  >  NZ_CP034943/2180099‑2180373
                                                                                                                                        |                                                                                                                                          
cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                                                                         <  1:1773441/140‑1 (MQ=255)
cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                                                                         <  2:1950828/140‑1 (MQ=255)
cAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                                                                         <  2:382249/140‑1 (MQ=255)
cAATGGTCATCATAATGACGAGTTGAACAAACATGAAAAAGAAAGATAAATGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCaaa                                                                                                                                         <  2:1950837/140‑1 (MQ=255)
   tGGTCATCCTAATGACGATGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGATGGCAATACACATAATGACCCTATAACAGAGCTTGAAGAAAGAGCCaaaaaa                                                                                                                                      <  1:1188285/140‑1 (MQ=255)
     gTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                                                                    <  1:813589/140‑1 (MQ=255)
         tCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAAt                                                                                                                                >  2:617742/1‑140 (MQ=255)
            tAATGACGAGGTGAACAAACATGAAAAAGAAAGATTAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACa                                                                                                                             <  2:650435/140‑1 (MQ=255)
             aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAACACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                            <  2:583626/140‑1 (MQ=255)
             aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                            <  2:1570290/140‑1 (MQ=255)
             aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                            <  2:1774419/140‑1 (MQ=255)
             aaTGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                            <  2:37935/140‑1 (MQ=255)
             aaTGACGAGGTGAACAAAAATGAAAAAGAAATATAAAGGCCGGCTGACCGTCTGTGTTACTCCTCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGGTTGAAGAAAGAGCCAAAAAAAGCAATACaa                                                                                                                            <  2:1680433/140‑1 (MQ=255)
                 aCGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACg                                                                                                                        <  1:881276/140‑1 (MQ=255)
                    aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                                                                     >  1:1391497/1‑140 (MQ=255)
                    aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                                                                     >  1:1391420/1‑140 (MQ=255)
                    aGGTGAACTAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTa                                                                                                                     >  1:556730/1‑140 (MQ=255)
                    aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                                                                     >  2:281554/1‑140 (MQ=255)
                    aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                                                                     >  2:274255/1‑140 (MQ=255)
                    aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                                                                     >  2:1218087/1‑140 (MQ=255)
                    aGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGAACGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTa                                                                                                                     >  2:1084762/1‑140 (MQ=255)
                          acaaacaTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGagaag                                                                                                               <  1:1443525/140‑1 (MQ=255)
                                aTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                                                                    >  1:546010/1‑113 (MQ=255)
                                aTGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAg                                                                                                                                    <  2:546010/113‑1 (MQ=255)
                                 tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                                                                        >  2:1793572/1‑140 (MQ=255)
                                 tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaca                                                                                                        >  2:2394731/1‑140 (MQ=255)
                                 tGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTaaa                                                                                                        >  2:2394062/1‑138 (MQ=255)
                                  gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTacac                                                                                                       <  1:1628492/140‑1 (MQ=255)
                                  gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTAGAGAGGATCTaaac                                                                                                       <  2:1391497/140‑1 (MQ=255)
                                  gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTAGAGAGGATCTaaac                                                                                                       <  2:1391420/140‑1 (MQ=255)
                                  gAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAAGCAATACAAAACGCTAGAGAGGATCTaaac                                                                                                       <  2:556730/140‑1 (MQ=255)
                                     aaagaaagATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAt                                                                                                    <  1:617742/140‑1 (MQ=255)
                                     aaagaaagATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAt                                                                                                    <  2:550330/140‑1 (MQ=255)
                                      aagaaagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATCCAAAACGCTAGAGAAGATCTACACGAtc                                                                                                   <  1:894280/140‑1 (MQ=255)
                                          aagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAtctctc                                                                                               >  2:709260/1‑140 (MQ=255)
                                          aagaTAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGAtctctc                                                                                               >  2:709686/1‑140 (MQ=255)
                                                 cggCCGGCTGACCGGCGGTGGTACTCCGCAACGCGATCGGGAAGGCAACACACAGAATGACCCTACCCCACCGCGCGAAGAAAGAGCCAAAAAAAGCAATACAACCCGCTAGAGAAGATCTACACGATCTCTCTAGCGtt                                                                                        <  2:2189941/139‑1 (MQ=255)
                                                    ccGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGACGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAACACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAg                                                                                     <  1:1021063/140‑1 (MQ=255)
                                                       gCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCtt                                                                                  >  1:1293697/1‑140 (MQ=255)
                                                       gCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCtt                                                                                  >  1:1610843/1‑140 (MQ=255)
                                                        cTGACCGGCGGTGTTACTCCGCAAGGCGACCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAGGAGAGAGCAAAAAAAGGCACTACAAAACGCTAGAGAGGATCTAAACGATCTCTCTAGCGTTCAGCttt                                                                                 >  2:1082915/1‑140 (MQ=255)
                                                           aCCGGCGGTGGTACTCCTCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAACAAAGCACTACAAAACGCTAGAGAAGATCTACACGATCTCTATAGCGTTCAGCTTTTCa                                                                              <  1:1004471/140‑1 (MQ=255)
                                                                 gggggtACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                                        <  1:1091786/135‑1 (MQ=255)
                                                                 ggTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                                        <  1:2167163/140‑1 (MQ=255)
                                                                 ggTGTTACTCCGCAAGGCGATCTGGAACGCCATAAACATAATGACCCTAACCCAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACCCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                                        <  1:966333/140‑1 (MQ=255)
                                                                     ttACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAACACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCt                                                                    <  2:824832/140‑1 (MQ=255)
                                                                       aCTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCa                                                                  <  1:1218087/140‑1 (MQ=255)
                                                                        cTCCGCAAGGCGATCGGGAAGGAAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATAAAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCaa                                                                 <  2:1440443/140‑1 (MQ=255)
                                                                             cAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCt                                                            >  1:1832268/1‑140 (MQ=255)
                                                                               aGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCttt                                                          >  2:682346/1‑140 (MQ=255)
                                                                                ggCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTc                                                                                       >  2:2411824/1‑110 (MQ=255)
                                                                                ggCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTc                                                                                       <  1:2411824/110‑1 (MQ=255)
                                                                                     tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGACGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg                                                    >  1:438463/1‑140 (MQ=255)
                                                                                     tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg                                                    >  1:452826/1‑140 (MQ=255)
                                                                                     tCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGAGCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtg                                                    >  1:222148/1‑140 (MQ=255)
                                                                                       tGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTtggt                                                  <  1:1462862/140‑1 (MQ=255)
                                                                                               aaTACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGa                                          <  1:1727929/140‑1 (MQ=255)
                                                                                               aaTACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGa                                          >  1:2068410/1‑140 (MQ=255)
                                                                                               aaTACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGa                                          >  1:2337243/1‑140 (MQ=255)
                                                                                               aaTACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGa                                          >  1:293670/1‑140 (MQ=255)
                                                                                                 tACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAg                                        >  1:2399558/1‑140 (MQ=255)
                                                                                                        aaTGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCa                                 >  2:1775380/1‑140 (MQ=255)
                                                                                                        aaTGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCa                                 >  2:1030388/1‑140 (MQ=255)
                                                                                                             cccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgc                            >  2:1942834/1‑140 (MQ=255)
                                                                                                             cccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgc                            >  2:1822408/1‑140 (MQ=255)
                                                                                                             cccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgc                            >  2:1527323/1‑140 (MQ=255)
                                                                                                              ccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgcc                           >  2:1711122/1‑140 (MQ=255)
                                                                                                              ccTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAgccgcc                           >  1:474924/1‑140 (MQ=255)
                                                                                                                tAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATGTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTGCTGTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTg                         >  1:1126783/1‑140 (MQ=255)
                                                                                                                tAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTTGTGGCGGCGAAGCATGTCAGCCGCCTg                         >  2:1654769/1‑140 (MQ=255)
                                                                                                                 aaaaCAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCATGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCAGGGCAGCCGCCTGc                        >  2:393277/1‑140 (MQ=255)
                                                                                                                   aaCAGAGCTTGAAGAAAGAGCCAAACAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTGTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTc                      <  2:274165/140‑1 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                                        <  2:1496616/85‑1 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                                        >  1:1496616/1‑85 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                                        >  1:1605202/1‑85 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATg                                                                        >  1:388777/1‑85 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt                 >  2:153343/1‑140 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt                 >  2:843217/1‑140 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt                 >  2:685812/1‑140 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt                 >  1:1966026/1‑140 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt                 >  1:2093596/1‑140 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt                 >  2:2179898/1‑140 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt                 >  2:1773192/1‑140 (MQ=255)
                                                                                                                        agCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCAttt                 >  2:204408/1‑140 (MQ=255)
                                                                                                                          cTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGTAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGc               <  2:1016812/140‑1 (MQ=255)
                                                                                                                             gaagaaAGAGCCAAACAAAGCACTACAAAACGCTAGAGAAGAACTACCCGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAgg            <  1:591959/140‑1 (MQ=255)
                                                                                                                             gaagaaAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAgg            <  1:2164029/140‑1 (MQ=255)
                                                                                                                             gaagaaAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAgg            <  1:682346/140‑1 (MQ=255)
                                                                                                                             gaagaaAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAgg            <  1:1463112/140‑1 (MQ=255)
                                                                                                                                  aagaGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTTCAGGGTGaa       >  1:1422541/1‑140 (MQ=255)
                                                                                                                                  aagaGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGaa       >  1:1102040/1‑140 (MQ=255)
                                                                                                                                  aagaGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGaa       >  1:996622/1‑140 (MQ=255)
                                                                                                                                  aagaGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGaa       >  1:159032/1‑140 (MQ=255)
                                                                                                                                  aagaGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATGTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGaa       >  1:491800/1‑140 (MQ=255)
                                                                                                                                    gagCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGAATa     >  1:2274100/1‑140 (MQ=255)
                                                                                                                                      gCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTCCACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGAATaaa   <  2:2076212/140‑1 (MQ=255)
                                                                                                                                      gCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGAATaaa   <  2:474924/140‑1 (MQ=255)
                                                                                                                                      gCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGAATaaa   <  2:151387/140‑1 (MQ=255)
                                                                                                                                      cccAAAAAAACCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGAATaaa   <  2:807503/139‑1 (MQ=255)
                                                                                                                                       ccAAAAACAGCAATACACACCGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGAATAAAg  <  1:1899229/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
CAATGGTCATCCTAATGACGAGGTGAACAAACATGAAAAAGAAAGATAAAGGCCGGCTGACCGGCGGTGTTACTCCGCAAGGCGATCTGGAAGGCAATACACATAATGACCCTAAAACAGAGCTTGAAGAAAGAGCCAAAAAAAGCAATACAAAACGCTAGAGAAGATCTACACGATCTCTCTAGCGTTCAGCTTTTCAAGCATGCGCTCAATTCCTTTGATTTGGTGGCGGCGAAGCATGTCAGCCGCCTGCTCCATTTGCAGGGTGAATAAAG  >  NZ_CP034943/2180099‑2180373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: