Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,294,428 G→T 5.5% G32G (GGC→GGA EO946_RS11665 ← YpuI family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,294,4280GT 5.5% 443.3 / 11.0 153G32G (GGC→GGAEO946_RS11665YpuI family protein
Reads supporting (aligned to +/- strand):  ref base G (77/66);  new base T (4/5);  total (82/71)
Fisher's exact test for biased strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

AATACTGTCATTCTTCTCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAGCGCGATT  >  NZ_CP034943/2294291‑2294567
                                                                                                                                         |                                                                                                                                           
aaTACTGTCATTCTTCTCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGcc                                                                                                                                           <  1:256227/140‑1 (MQ=255)
aaTACTGTCATTCTTCTCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGcc                                                                                                                                           <  1:67887/140‑1 (MQ=255)
aaTACTGTCATTCTTCTCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGcc                                                                                                                                           >  1:1638285/1‑140 (MQ=255)
  tACTGTCATTCTTCTCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTg                                                                                                                                         <  1:118223/140‑1 (MQ=255)
  tACTGTCATTCTTCTCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTg                                                                                                                                         <  1:118699/140‑1 (MQ=255)
  tACTGTCATTCTTCTCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTg                                                                                                                                         <  1:820087/140‑1 (MQ=255)
            cttctCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGt                                                                                                                               >  1:1153684/1‑140 (MQ=255)
            cttctCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGGATTGACAACACTTGATAAGAATTCGCCTGTTTTTTTAgg                                                                                                                               >  1:1583450/1‑139 (MQ=255)
               ctcAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTGTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTg                                                                                                                            >  1:13369/1‑140 (MQ=255)
               ctcAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTGTGATTTAGGTAATCGTTAACCGTATTGACCACCATTGATAAGAATTGGCCTGTTTTTTGTGTCTg                                                                                                                            >  1:1849247/1‑140 (MQ=255)
                    agTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTc                                                                                                                       >  2:1534025/1‑140 (MQ=255)
                        cTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTTATTTTGGTAATCGTTAAAGGTATTGACAACCATTGATAAGAATTCGCCGGTTTTTTGATTATGcgcacgcaa                                                                                                                   >  1:1398318/1‑139 (MQ=255)
                        cTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTGTTTTGAGTCTGCGCTCGcac                                                                                                                   >  1:473974/1‑140 (MQ=255)
                         tCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTTATTTAGGTAATCGTTAACGGTATTGACAACCATTGATCAGACTTCTCCTGTTTTTTGAGTCTGCGCTCGcaca                                                                                                                  >  2:1446637/1‑140 (MQ=255)
                         tCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTTACCACCATTGATAAGAATTCTCCTGTTTTTTGAGTCTGCGCTCGcaca                                                                                                                  >  2:1293752/1‑140 (MQ=255)
                         tCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCTCCTGTTTTTTGAGTCTGCGCTCGcaca                                                                                                                  >  2:1294067/1‑140 (MQ=255)
                         tCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGAGTTAGGGAATCGTTAACGGTATTGACAACCATTGAAAAGAATTCGACGGTTTTTTGAGTCTGCGCTCGcaaa                                                                                                                  >  2:9689/1‑138 (MQ=255)
                             cacaTCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCg                                                                                                              >  1:2390265/1‑140 (MQ=255)
                                aTCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAAAGGTATTGACAACCCTTGCTAAGAATTCGCCTGTTTTTTGAGTGTGCGCTCGCACAATCGAAt                                                                                                           >  1:1286261/1‑140 (MQ=255)
                                  cacaGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTGTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTTAGTCTGGGCTCGCACAATCGAATGt                                                                                                         >  2:777214/1‑140 (MQ=255)
                                     aGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCg                                                                                                                      >  1:983983/1‑124 (MQ=255)
                                     aGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTccc                                                                                                      >  2:232536/1‑140 (MQ=255)
                                     aGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTccc                                                                                                      >  2:142973/1‑140 (MQ=255)
                                      gCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCa                                                                                                     <  1:2266976/140‑1 (MQ=255)
                                       cAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCAt                                                                                                    >  2:274513/1‑140 (MQ=255)
                                       cAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCAt                                                                                                    >  2:1769338/1‑140 (MQ=255)
                                       cAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCAt                                                                                                    >  2:866854/1‑140 (MQ=255)
                                       cAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAAATCGCCTGTTTTTTGTGTCTGCGCTCGCACAATCGACTGTCCCAt                                                                                                    >  2:2357906/1‑140 (MQ=255)
                                       cAATATCCTGTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCAt                                                                                                    >  2:562732/1‑140 (MQ=255)
                                        aaTATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCAtt                                                                                                   <  1:1006978/140‑1 (MQ=255)
                                          tatCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTTTTTTAGGTAATAGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTTAGTCTGCGCTCGCACAATCGAATGTCCCATTTc                                                                                                 >  1:705307/1‑140 (MQ=255)
                                          tatCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTTATTTATGTAATAGTTAACTGTATTTACAAACAATGAAAAAAATTCGCCAGTTTTTTTATTCTGCGCTCGCACAAAACAAAGTACCAttta                                                                                                 >  1:1415964/1‑139 (MQ=255)
                                          tatCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAAATCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTc                                                                                                 >  1:1644172/1‑140 (MQ=255)
                                          tatCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGAGAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCAAAATAGAATGTCCCATTTc                                                                                                 >  2:1598491/1‑140 (MQ=255)
                                              cTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGttt                                                                                             <  2:2182710/140‑1 (MQ=255)
                                                 tctcAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGTTTTAGGTAATCGTTAACGGTATTGACAACCAATTATAAGAATTAGCCTGTTTTTTTATTCTGCGCTCGCACAAACGAATGTCCCATTTCGGTTGGt                                                                                          >  1:1041292/1‑140 (MQ=255)
                                                 tctcAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGTTGTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGt                                                                                          >  1:354851/1‑140 (MQ=255)
                                                 tctcAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGt                                                                                          >  2:744240/1‑140 (MQ=255)
                                                      gTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCtt                                                                                     <  1:1008730/140‑1 (MQ=255)
                                                       tAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGTTTTAGGTAATCGTTAACGGTATTGACAACCAGTTATAAGAATTCGCCTGTTTTTTGAGTGTGGGCTCGCACAAACGAATGTCCCCATTCGTTTGGTATCttg                                                                                    >  1:198060/1‑139 (MQ=255)
                                                       tAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGTTGTAGGTAATCGTTAACGGTATTGACAGCCATTGATAAGAATTCGGCTGTGTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTCGCTTc                                                                                    >  2:1728803/1‑140 (MQ=255)
                                                          gCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTTATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTGTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTcccc                                                                                 >  2:72664/1‑140 (MQ=255)
                                                          gCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAAATCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTcccc                                                                                 >  2:73455/1‑140 (MQ=255)
                                                            aaTTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTc                                                                               <  2:2116508/140‑1 (MQ=255)
                                                             aTTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCa                                                                              <  2:473974/140‑1 (MQ=255)
                                                                   ccTGTAAGGACTCCAGCGTGGTGTGATTTATGTAATCGTTAACCGTATTTACAACCATTGATAAGAATTCGGCGGTTTTTTGAGTGTGCGCGCGGACAATCGAATTTCCCATTTCGTTTGGTTTGTTGGCCTGATGTGtt                                                                        >  1:424132/1‑140 (MQ=255)
                                                                   ccTGTAAGGACTCCAGCGTCGTTTTATTTAGGGAATCGTTAACGGTATTTACACGCATTGATAAGAATTCGCCTGTGTTTTGTGTCTGGGCTCGCGCAATCGGATGTCCCATTTGGTTTTGTGGGTTGCCCTCAGGTGtt                                                                        >  1:1884252/1‑140 (MQ=255)
                                                                   ccTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTTACAACCAATGATAAGAATTAGCCTGTTTTTTTAGTCTGCGCTCGCACAAACGAATTTCCCCTTTCGTTTTGTATCTTCCCCTCATGTCtt                                                                        >  1:1884429/1‑140 (MQ=255)
                                                                    cTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCttt                                                                       <  2:1952671/140‑1 (MQ=255)
                                                                    cTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCttt                                                                       <  2:1519213/140‑1 (MQ=255)
                                                                    cTGTAAGGACTCCAGCGTCGATTGATTTAGGTAATCGTTAACGGGATTGACAACCAGTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCttt                                                                       <  2:604017/140‑1 (MQ=255)
                                                                          ggACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGtctct                                                                 <  1:1769338/140‑1 (MQ=255)
                                                                          ggACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGtctct                                                                 <  1:562732/140‑1 (MQ=255)
                                                                               ccAGCGTCGTTTGATTTAGGTACTCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGCGTCTGCGCTCGCACAAACGAATGTCCCATTTGGTTTGGTATATTCCCCTCATGTCTTTGTCTGTCAGAt                                                            >  2:2019071/1‑140 (MQ=255)
                                                                               ccAGCGTCGTTTGATTTAGGTAATCCTTAACGGTATTTACAAACAATGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTAAAGTCTTTGTATCTAACAt                                                            >  2:2022376/1‑140 (MQ=255)
                                                                                cAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACAtt                                                           <  1:1069825/140‑1 (MQ=255)
                                                                                cAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAAATCGCCTGTTTTTTGAGTCTGGGCTCGCACAATCGAATGTACCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACAtt                                                           >  2:2197745/1‑140 (MQ=255)
                                                                                    gtcgtTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGc                                                       <  2:2390265/140‑1 (MQ=255)
                                                                                     tcgtTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGct                                                      <  2:1644172/140‑1 (MQ=255)
                                                                                     tcgtTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGct                                                      <  2:705307/140‑1 (MQ=255)
                                                                                     tcgtTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAAATCGCCTGTGTTTTTAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTCTCTTCCCCTCATTTCTTTGTGTCTCACATTTTGct                                                      >  2:2172192/1‑140 (MQ=255)
                                                                                        tttGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTGTTTGTCTCGCACATTTTGcttct                                                   >  2:529538/1‑140 (MQ=255)
                                                                                        tttGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGcttct                                                   <  1:568592/140‑1 (MQ=255)
                                                                                        tttGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAAACGAATGTCCCAGTTCGTTTGGTATCTTCCCCTCATTTCTTTGTCTCTCACATTTTGcttat                                                   >  2:1170051/1‑138 (MQ=255)
                                                                                               tAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCCCAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTTTCTCTCACCTTTTGCTTCTTTCATGa                                            >  1:125199/1‑140 (MQ=255)
                                                                                                     aTCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGctt                                      <  1:232536/140‑1 (MQ=255)
                                                                                                     aTCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGctt                                      <  2:1041292/140‑1 (MQ=255)
                                                                                                     aTCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGctt                                      <  2:1876174/140‑1 (MQ=255)
                                                                                                      tCGTTAACTGTATTGACAACCATTGATAAGAAGTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCCCCTTTTGCTTCTTTCATGATAGcttc                                     <  2:39949/140‑1 (MQ=255)
                                                                                                      tCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGcttc                                     <  1:2211194/140‑1 (MQ=255)
                                                                                                      tCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGcttc                                     <  1:1855833/140‑1 (MQ=255)
                                                                                                      tCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGcttc                                     >  1:902318/1‑140 (MQ=255)
                                                                                                      ccgctcaCGGTATGGACAACCAATCATAAGAATTCGCCTGTTTATTGAGTCTGAGCTCGCACAATAGACTGTCCCATTTCGTTTGGTATCTTCCCCTCATCTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGcttc                                     <  1:1981678/134‑1 (MQ=255)
                                                                                                       cGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGcttct                                    <  2:1849247/140‑1 (MQ=255)
                                                                                                       cGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGcttct                                    <  2:13369/140‑1 (MQ=255)
                                                                                                       agTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGcttct                                    <  2:776295/139‑1 (MQ=255)
                                                                                                           aaCGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCt                                >  1:1711318/1‑140 (MQ=255)
                                                                                                           aaCGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCt                                <  1:1526451/140‑1 (MQ=255)
                                                                                                           aaCGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTGTGCTTCTTTCATGATAGCttcttcgt                                >  1:161670/1‑138 (MQ=255)
                                                                                                            aCGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCtt                               <  2:345839/140‑1 (MQ=255)
                                                                                                            aCGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCtt                               <  1:182033/140‑1 (MQ=255)
                                                                                                            aCGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCGTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCtt                               <  1:914499/140‑1 (MQ=255)
                                                                                                              ggTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTtat                             <  2:1973644/140‑1 (MQ=255)
                                                                                                                tATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCAGATTTTGCTTCTTTCATGATAGCTGCTTCCTtatct                           >  2:1245494/1‑138 (MQ=255)
                                                                                                                tATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATATCTTCTTCCTTatat                           >  2:947925/1‑140 (MQ=255)
                                                                                                                 aTTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAAACGAATGTCCCATTTCGTTTGGTATCTTCCACTCATGTCTTTGTATCTCACATTTTGCTTATTTCATGATAGCTTCTTCCTTATATc                          >  2:2072889/1‑140 (MQ=255)
                                                                                                                       aaCCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTatga                    >  1:1527549/1‑140 (MQ=255)
                                                                                                                       aaCCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTatga                    <  1:2758/140‑1 (MQ=255)
                                                                                                                          cATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTatgatga                 >  2:2227909/1‑140 (MQ=255)
                                                                                                                          cATTGATAAGAATTCGCCTGTTGTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTatgatga                 >  2:1105504/1‑140 (MQ=255)
                                                                                                                            ttGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCAGATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTatgatgatg               >  1:1799656/1‑140 (MQ=255)
                                                                                                                            ttGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTatgatgatg               >  1:1130110/1‑140 (MQ=255)
                                                                                                                                    aaTTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAgcg       >  2:1819094/1‑140 (MQ=255)
                                                                                                                                      ttCTCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAGcgcg     <  1:1293752/140‑1 (MQ=255)
                                                                                                                                      ttCTCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAGcgcg     <  1:1756377/140‑1 (MQ=255)
                                                                                                                                      ttCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAGcgcg     <  2:214389/140‑1 (MQ=255)
                                                                                                                                      ttCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAGcgcg     <  1:1247927/140‑1 (MQ=255)
                                                                                                                                       tCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAGCGCGa    <  2:1379673/140‑1 (MQ=255)
                                                                                                                                       tCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGATTCTTCCTTATATCTTATGATGATGAAAAAGCGCGa    <  2:2328011/140‑1 (MQ=255)
                                                                                                                                         gCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAGCGCGAtt  <  1:221324/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
AATACTGTCATTCTTCTCAGAGTGCTCAGCACATCACAGCAATATCCTTTCTCAGTAGGCAATTCAGCCTGTAAGGACTCCAGCGTCGTTTGATTTAGGTAATCGTTAACGGTATTGACAACCATTGATAAGAATTCGCCTGTTTTTTGAGTCTGCGCTCGCACAATCGAATGTCCCATTTCGTTTGGTATCTTCCCCTCATGTCTTTGTCTCTCACATTTTGCTTCTTTCATGATAGCTTCTTCCTTATATCTTATGATGATGAAAAAGCGCGATT  >  NZ_CP034943/2294291‑2294567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: