Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,601,897 T→A 5.2% S37S (TCA→TCT EO946_RS13465 ← penicillin‑binding protein 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,601,8970TA 5.2% 254.6 / 4.5 77S37S (TCA→TCTEO946_RS13465penicillin‑binding protein 2
Reads supporting (aligned to +/- strand):  ref base T (36/37);  new base A (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

AACAACCGCAGGCTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACATTGTGGACG  >  NZ_CP034943/2601765‑2602036
                                                                                                                                    |                                                                                                                                            
aacaacCGCAGGCTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATg                                                                                                                                       >  2:388279/1‑140 (MQ=255)
   aacCGCAGGCTCGCTTTTGCCGGTCAGCGCCTGCCCGTGTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGAtt                                                                                                                                    <  1:1721289/140‑1 (MQ=255)
     ccGCAGGCTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCt                                                                                                                                  >  1:2144015/1‑140 (MQ=255)
        cgggCTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTa                                                                                                                              >  1:2165580/3‑140 (MQ=255)
          ggCTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTaaa                                                                                                                            >  1:2379554/1‑140 (MQ=255)
          ggCTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTaaa                                                                                                                            >  1:1271932/1‑140 (MQ=255)
           gCTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCTTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTaaaa                                                                                                                           <  2:2170668/140‑1 (MQ=255)
            cTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTaaaaa                                                                                                                          <  1:992562/140‑1 (MQ=255)
             tCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTaaaaaa                                                                                                                         >  2:59461/1‑140 (MQ=255)
                 ttttGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGc                                                                                                                     <  2:951228/140‑1 (MQ=255)
                 ttttGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGc                                                                                                                     <  2:1339234/140‑1 (MQ=255)
                 tttgGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGAACAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGc                                                                                                                     <  2:2431726/140‑1 (MQ=255)
                         gTCAGCGCCTGCCCGTTTCGATCTAAAATGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTc                                                                                                             >  1:217296/1‑140 (MQ=255)
                           cAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCg                                                                                                           <  1:1166737/140‑1 (MQ=255)
                                    cccGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTca                                                                                                  >  2:614380/1‑140 (MQ=255)
                                    cccGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTca                                                                                                  >  2:653049/1‑140 (MQ=255)
                                    cccGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTca                                                                                                  >  2:1540053/1‑140 (MQ=255)
                                        tttCTATGTAAAAAGGAACATCTTCCGTCAGAAATGAGTACTTCCTCCGTACTCTGTTTCACGCTGTCCTGGATCAAATGGATGTTCTTTTTTGAAAGTGATTGTGTAAAAAACAGCTGGATTTCCGCCAACCTcagcag                                                                                              >  2:191830/1‑140 (MQ=255)
                                         ttCGATCTAAAAAAGAGCCTCTTCCATCTGAAATGAGCACTTCCTCCGTCCGCTGTTTCACACTTTCTTGAATCAAATTAATTTTCTTTTTAGAAAATGATTCTGTGAAAAACAGCTGGATTTCTGCAAGTCTCAGCAGa                                                                                             >  2:369265/1‑140 (MQ=255)
                                         ttCGATCTAAAAAAGAGCCTCTTCCATCTGAAATGAGCACTTCCTCCGTCCGCTGTTTCACACTTTCTTGAATCAAATTAATTTTCTTTTTAGAAAATGATTCTGTGAAAAACAGCTGGATTTCTGCAAGTCTCAGCAGa                                                                                             >  2:2031568/1‑140 (MQ=255)
                                           cGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAg                                                                                           <  2:898871/140‑1 (MQ=255)
                                           cGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGCATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTACAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAg                                                                                           <  1:1671776/140‑1 (MQ=255)
                                           cGATCTAAAAACGAACCTCTTCCGTCAGACATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAg                                                                                           <  2:1455880/140‑1 (MQ=255)
                                            gATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGc                                                                                          <  1:2164536/140‑1 (MQ=255)
                                                        aaCCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTACTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGACAAAAACg                                                                              >  2:542185/1‑140 (MQ=255)
                                                          ccTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGAt                                                                            <  2:21069/140‑1 (MQ=255)
                                                          ccTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGAt                                                                            <  2:1057698/140‑1 (MQ=255)
                                                              ttCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCaaa                                                                        <  1:460982/140‑1 (MQ=255)
                                                              ttCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCaaa                                                                        <  1:1801411/140‑1 (MQ=255)
                                                                      gAAATTAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTTGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGTATTTCCGCCAACCTCAGCAGAAGCAGTAAAAAAACGATCAAAAAAGCAAt                                                                <  2:1668478/140‑1 (MQ=255)
                                                                            aGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGc                                                          >  1:343604/1‑140 (MQ=255)
                                                                                 ttCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCt                                                     >  2:1240069/1‑140 (MQ=255)
                                                                                 ttCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCt                                                     >  2:703845/1‑140 (MQ=255)
                                                                                 ttCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATAAAAAAAGCAATGACTGCCAGCt                                                     >  2:374527/1‑140 (MQ=255)
                                                                                   cctccGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCGGGTAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTc                                                   >  2:1730463/1‑140 (MQ=255)
                                                                                   cctccGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTc                                                   >  1:2111232/1‑140 (MQ=255)
                                                                                   cctccGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTc                                                   >  1:848948/1‑140 (MQ=255)
                                                                                   cctccGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGATGGATTTCCGCCAACCTCAGAAGAAGCAGGCCAAAAACGATCAAAAAAGCAATGACTGCCAGCTTc                                                   >  2:1491811/1‑140 (MQ=255)
                                                                                     tccgtccgCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCAt                                                 <  2:2111232/140‑1 (MQ=255)
                                                                                     tccgtccgCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCAt                                                 <  2:848948/140‑1 (MQ=255)
                                                                                      ccgtccgCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAATTGATTCTGTAAAAAACAGCTGGAGTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAACAGCAATGAGTGCCAGCTTCAtt                                                >  2:52727/1‑140 (MQ=255)
                                                                                      ccgtccgCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCCtt                                                >  1:328794/1‑140 (MQ=255)
                                                                                      ccgtccgCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAAAAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCAtt                                                >  1:1543752/1‑140 (MQ=255)
                                                                                          ccgCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGACGCAGGAAAAAAACGATCAAAAAAGCACTGACTGCCAGCTTCATTCGtg                                            >  2:295960/1‑139 (MQ=255)
                                                                                          ccgCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGtt                                            >  2:1192149/1‑140 (MQ=255)
                                                                                              tGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGTAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAAAGATCAAAAAATCAATGACTGCCAGCTTCATTCGTTTCGa                                        >  2:1358937/1‑140 (MQ=255)
                                                                                              tGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGa                                        >  1:1115966/1‑140 (MQ=255)
                                                                                              tGTTTCACGCTATCCTGGATCAAATTGATATTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAAACTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGACAGCTACAGTCTTTTAGa                                        >  2:2178297/1‑140 (MQ=255)
                                                                                               gTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGat                                       <  2:1115966/140‑1 (MQ=255)
                                                                                                   cACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCt                                   >  2:1704129/1‑140 (MQ=255)
                                                                                                   cACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCt                                   >  2:605663/1‑140 (MQ=255)
                                                                                                    aagCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCtt                                  <  2:1271932/138‑1 (MQ=255)
                                                                                                    aCGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCtt                                  <  2:2379554/140‑1 (MQ=255)
                                                                                                          tCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATaaa                            >  1:2043746/1‑140 (MQ=255)
                                                                                                          tCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATaaa                            >  1:800679/1‑140 (MQ=255)
                                                                                                               gATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAac                       <  1:1042193/140‑1 (MQ=255)
                                                                                                                 tCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAacac                     <  1:614380/140‑1 (MQ=255)
                                                                                                                 tCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAacac                     <  1:1540053/140‑1 (MQ=255)
                                                                                                                 tCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAacac                     <  1:653049/140‑1 (MQ=255)
                                                                                                                   aaaTTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCt                   <  2:2144015/140‑1 (MQ=255)
                                                                                                                    aaTTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCt                   <  1:2269689/140‑1 (MQ=255)
                                                                                                                    aaTTGATTTTCTTTTATGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACTATCAAAAAAGCAATGACTGCCAGCTTAATTCGTTTCGATATCTTAATAAAAAAACACCTc                  >  1:933271/1‑140 (MQ=255)
                                                                                                                     aTTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGAATGCCAGCTTCATTCGTTTCGATCTCTTAATAAAAAAACAACTCa                 >  2:1395382/1‑140 (MQ=255)
                                                                                                                       tGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCAcc               <  1:407013/140‑1 (MQ=255)
                                                                                                                        aaTTTTCTTTTTAGAAAATGATTCTTTTAAAAACAGATGGATTTCTGCAAGTCTCAGCAGAAGCAGAAAAAATACAATCAAAAAAGCGATGACTGCCAGCTTAATTCGTTTCGATATCTTCATAAAAAAACACCTCAccc              <  1:369265/139‑1 (MQ=255)
                                                                                                                        aaTTTTCTTTTTAGAAAATGATTCTGTGAAAAACAGCTGGATTTCTGCAAGTCTCAGCAGAAGCAGAAAAAATACAATCAAAAACGCGATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCAccc              <  1:2031568/139‑1 (MQ=255)
                                                                                                                         aTTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCca             <  1:605663/140‑1 (MQ=255)
                                                                                                                         aTTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCca             <  1:1704129/140‑1 (MQ=255)
                                                                                                                         aTTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCca             <  1:1419259/140‑1 (MQ=255)
                                                                                                                            ttCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACAt          <  2:800679/140‑1 (MQ=255)
                                                                                                                            ttCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACAt          <  2:2043746/140‑1 (MQ=255)
                                                                                                                              cTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGc                                                                                          >  2:1028489/1‑58 (MQ=255)
                                                                                                                              cTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGc                                                                                          <  1:1028489/58‑1 (MQ=255)
                                                                                                                              cTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGc                                                                                          <  1:2065958/58‑1 (MQ=255)
                                                                                                                              cTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGc                                                                                          >  2:2065958/1‑58 (MQ=255)
                                                                                                                              cTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACATtg        <  1:392769/140‑1 (MQ=255)
                                                                                                                              cTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACATtg        <  1:362998/140‑1 (MQ=255)
                                                                                                                                  tttGACAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACATTGTGGa    <  1:2423910/140‑1 (MQ=255)
                                                                                                                                    tGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACATTGTGGACg  <  1:901160/140‑1 (MQ=255)
                                                                                                                                    tGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACATTGTGGACg  <  1:954815/140‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                            
AACAACCGCAGGCTCGCTTTTGCCGGTCAGCGCCTGCCCGTTTCGATCTAAAAAGGAACCTCTTCCGTCAGAAATGAGTACTTCCTCCGTCCGCTGTTTCACGCTTTCCTGGATCAAATTGATTTTCTTTTTTGAAAATGATTCTGTAAAAAACAGCTGGATTTCCGCCAACCTCAGCAGAAGCAGGAAAAAAACGATCAAAAAAGCAATGACTGCCAGCTTCATTCGTTTCGATATCTTCATAAAAAAACACCTCACCCACATTGTGGACG  >  NZ_CP034943/2601765‑2602036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: