Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,611,703 T→G 5.1% intergenic (‑322/+12) alaS ← / ← EO946_RS13520 alanine‑‑tRNA ligase/AI‑2E family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,611,7030TG 5.1% 522.7 / 9.9 164intergenic (‑322/+12)alaS/EO946_RS13520alanine‑‑tRNA ligase/AI‑2E family transporter
Reads supporting (aligned to +/- strand):  ref base T (90/65);  new base G (4/5);  total (94/70)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

CACTCAGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAT  >  NZ_CP034943/2611566‑2611840
                                                                                                                                         |                                                                                                                                         
cACTCAGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGc                                                                                                                                         >  2:1523188/1‑140 (MQ=255)
cACTCAGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGc                                                                                                                                         >  2:1996976/1‑140 (MQ=255)
  cTCAGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCAc                                                                                                                                       >  2:933622/1‑140 (MQ=255)
  cTCAGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCAc                                                                                                                                       >  2:211234/1‑140 (MQ=255)
  cTCAGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCAc                                                                                                                                       >  2:2126937/1‑140 (MQ=255)
     aGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGcc                                                                                                                                    <  1:747003/140‑1 (MQ=255)
     aGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGcc                                                                                                                                    <  1:832313/140‑1 (MQ=255)
       gATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                  <  2:1441516/140‑1 (MQ=255)
       gATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                  <  2:1905372/140‑1 (MQ=255)
       gATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                  <  2:350396/140‑1 (MQ=255)
       gATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                  <  2:1929207/140‑1 (MQ=255)
       gATTAGCTTCCTCCGATCTTCTGAGAAAGCTCCTGTCAGCTTCCGGAGCTTCTCACTTTCACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                  <  1:1056679/140‑1 (MQ=255)
               tcctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATg                                                                                                                          <  2:2118429/140‑1 (MQ=255)
                 ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACACGCTGCACGCCAATGTCAATGCt                                                                                                                        >  2:1471129/1‑140 (MQ=255)
                 ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                        >  2:2176714/1‑140 (MQ=255)
                 ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                        <  1:2080308/140‑1 (MQ=255)
                 ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                        >  2:2291615/1‑140 (MQ=255)
                 ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                        <  1:1626910/140‑1 (MQ=255)
                 ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAAGGCt                                                                                                                        <  1:1447670/140‑1 (MQ=255)
                 ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCACATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAACCTGCACGCCAATGTCAATGCt                                                                                                                        >  2:316870/1‑140 (MQ=255)
                 ctccGATCTTCTGATAAAGCTCATTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                        <  2:220256/140‑1 (MQ=255)
                                     tCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTaaaaaa                                                                                                    >  2:2007531/1‑140 (MQ=255)
                                      ccTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTaaaaaag                                                                                                   >  2:416342/1‑139 (MQ=255)
                                                 aCGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                        >  1:1017227/1‑140 (MQ=255)
                                                 aCGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                        >  1:1109343/1‑140 (MQ=255)
                                                 aCGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                        <  2:1705953/140‑1 (MQ=255)
                                                       cTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACtttt                                                                                  >  2:302353/1‑140 (MQ=255)
                                                               tttAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGc                                                                          >  2:511856/1‑140 (MQ=255)
                                                               tttAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGc                                                                          >  2:235341/1‑140 (MQ=255)
                                                                 tAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCgg                                                                        >  1:1936299/1‑140 (MQ=255)
                                                                 tAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCgg                                                                        >  1:336193/1‑140 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATTTATTCGACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCTCAg                                                                  >  1:190764/1‑140 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                        >  1:389445/1‑118 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                        >  1:11956/1‑118 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                        <  2:389445/118‑1 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                        <  2:11956/118‑1 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAg                                                                  >  2:1483270/1‑140 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAg                                                                  >  1:1028521/1‑140 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAg                                                                  >  1:1373512/1‑140 (MQ=255)
                                                                       ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAg                                                                  >  1:1411716/1‑140 (MQ=255)
                                                                         cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACACGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                >  2:763792/1‑140 (MQ=255)
                                                                         cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                >  1:105012/1‑140 (MQ=255)
                                                                         cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                <  2:577971/140‑1 (MQ=255)
                                                                         cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                <  2:2151345/140‑1 (MQ=255)
                                                                         cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGa                                                                >  2:499582/1‑140 (MQ=255)
                                                                         cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGa                                                                >  2:499552/1‑140 (MQ=255)
                                                                         cTTCGGCGTACTCAGATACGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                <  2:2120481/140‑1 (MQ=255)
                                                                          ttCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGaa                                                               <  1:234589/140‑1 (MQ=255)
                                                                          ttCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATGATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGaa                                                               <  2:261992/140‑1 (MQ=255)
                                                                          ttCGGCGTACTCAGATCCGTCAATGGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGaa                                                               <  2:2318408/140‑1 (MQ=255)
                                                                            cGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACa                                                             >  2:1630077/1‑140 (MQ=255)
                                                                            cGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCAGCACTTTTAAAACAGCGGTCGCAGGAACa                                                             >  2:1440599/1‑140 (MQ=255)
                                                                             ggCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAg                                                            >  2:182783/1‑140 (MQ=255)
                                                                                   cTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGaa                                                      >  2:2053933/1‑140 (MQ=255)
                                                                                        aTCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAACTGGATCCTCATCACTTTTAACACAGCGGTCGCAGGAACAGCCAGAATCAtt                                                 >  1:710461/1‑140 (MQ=255)
                                                                                        aTCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCAtt                                                 >  1:1812928/1‑140 (MQ=255)
                                                                                        aTCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCAtt                                                 >  2:2309892/1‑140 (MQ=255)
                                                                                            gTCACTCGAGATTCGTATGTATTCTACAAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCaa                                             <  1:315729/140‑1 (MQ=255)
                                                                                             tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCCATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                            >  2:600611/1‑140 (MQ=255)
                                                                                             tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                            >  1:942225/1‑140 (MQ=255)
                                                                                             tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                            >  2:211054/1‑140 (MQ=255)
                                                                                             tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                            >  2:871243/1‑140 (MQ=255)
                                                                                             tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                            >  2:2186329/1‑140 (MQ=255)
                                                                                               aCTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGa                                          <  1:2053933/140‑1 (MQ=255)
                                                                                               aCTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGa                                          <  1:168887/140‑1 (MQ=255)
                                                                                                 tCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGAtt                                        <  2:1713820/140‑1 (MQ=255)
                                                                                                  cGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTc                                       >  1:90471/1‑140 (MQ=255)
                                                                                                      aTTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                   <  1:1708737/140‑1 (MQ=255)
                                                                                                      aTTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                   <  2:2418653/140‑1 (MQ=255)
                                                                                                         cGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACCGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAg                                <  2:1333461/140‑1 (MQ=255)
                                                                                                         cGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAg                                <  2:110176/140‑1 (MQ=255)
                                                                                                                    tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGACCAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCgg                     >  2:1157247/1‑140 (MQ=255)
                                                                                                                    tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGa                                                       >  1:1194195/1‑106 (MQ=255)
                                                                                                                    tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGa                                                       <  2:73189/106‑1 (MQ=255)
                                                                                                                    tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCgg                     >  1:2120140/1‑140 (MQ=255)
                                                                                                                    tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCgg                     >  1:2333561/1‑140 (MQ=255)
                                                                                                                    tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGAGTCCCGCAAGCTCTCCTCCgg                     >  2:2272359/1‑140 (MQ=255)
                                                                                                                      cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                   <  2:2175520/124‑1 (MQ=255)
                                                                                                                      cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                   >  1:2175520/1‑124 (MQ=255)
                                                                                                                      cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCa                   >  1:594116/1‑140 (MQ=255)
                                                                                                                      cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCa                   >  1:2206856/1‑140 (MQ=255)
                                                                                                                      cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCa                   >  1:917471/1‑140 (MQ=255)
                                                                                                                        agaAATTCTAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGAGTCCCGCAAGCTCTCCTCCGGCAAg                 <  2:826864/140‑1 (MQ=255)
                                                                                                                        agaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                 <  2:1268302/140‑1 (MQ=255)
                                                                                                                        agaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                 <  1:766207/140‑1 (MQ=255)
                                                                                                                        agaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                 <  2:171146/140‑1 (MQ=255)
                                                                                                                        agaAATTATAGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGAACAGCCAGAATCATCCCGACGATACCCGCAAGCTCCCCTCCGGCAAg                 <  1:499552/140‑1 (MQ=255)
                                                                                                                         gaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGc                >  1:2034189/1‑140 (MQ=255)
                                                                                                                         gaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGc                >  1:1222742/1‑140 (MQ=255)
                                                                                                                         caaaTTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGc                <  2:1297700/139‑1 (MQ=255)
                                                                                                                              tataGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGc           <  1:2176714/140‑1 (MQ=255)
                                                                                                                              tataGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGAACAGCCAGAATCATCCCGACGATACCCGCAAGCTCCCCTCCGGCAAGGAGTGc           <  1:235341/140‑1 (MQ=255)
                                                                                                                                       gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGc           <  2:678049/131‑1 (MQ=255)
                                                                                                                                       gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGc           >  1:678049/1‑131 (MQ=255)
                                                                                                                                       gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt  >  1:1564331/1‑140 (MQ=255)
                                                                                                                                       gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt  >  2:587218/1‑140 (MQ=255)
                                                                                                                                       gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt  >  2:1559026/1‑140 (MQ=255)
                                                                                                                                       gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt  >  1:2411394/1‑140 (MQ=255)
                                                                                                                                       gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt  >  1:456814/1‑140 (MQ=255)
                                                                                                                                       gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCACTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt  >  1:2385258/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                         
CACTCAGGATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAT  >  NZ_CP034943/2611566‑2611840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: