Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 438,588 C→G 5.8% L49L (CTG→CTC gabR ← transcriptional regulator GabR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943438,5880CG 5.8% 598.3 / 21.3 183L49L (CTG→CTCgabRtranscriptional regulator GabR
Reads supporting (aligned to +/- strand):  ref base C (66/106);  new base G (4/7);  total (70/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 186 total aligned reads displayed.

GCAGTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTG  >  NZ_CP034943/438449‑438726
                                                                                                                                           |                                                                                                                                          
gCAGTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTc                                                                                                                                            >  1:2273208/1‑140 (MQ=255)
gCAGTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTc                                                                                                                                            >  1:1282520/1‑140 (MQ=255)
 cAGTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCGTCTCCACGAAAAACCCCTTTCGCGCAATGGCGTACAAATATCCCACAGCCAGAATCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCa                                                                                                                                           <  1:548216/140‑1 (MQ=255)
   gTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGc                                                                                                                                         >  1:37594/1‑140 (MQ=255)
    tGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCt                                                                                                                                        >  1:2075391/1‑140 (MQ=255)
           ggggggTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGc                                                                                                                                 <  1:1053179/140‑1 (MQ=255)
           ggggggTGCTCCACGGCAGAAAACAGGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACACATAGCCCTCAGCCAGAAGCTGCTGATAGGCGGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGc                                                                                                                                 <  1:1205277/140‑1 (MQ=255)
            gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                >  1:100111/3‑140 (MQ=255)
            gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                >  1:397235/3‑140 (MQ=255)
            gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                >  1:621177/3‑140 (MQ=255)
              gggTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAg                                                                                                                              >  2:524815/1‑140 (MQ=255)
                gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                            >  2:1346434/1‑140 (MQ=255)
                gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                            <  1:1402104/140‑1 (MQ=255)
                gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                            >  2:448435/1‑140 (MQ=255)
                gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                            <  1:159771/140‑1 (MQ=255)
                gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                            <  2:925159/140‑1 (MQ=255)
                gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                            <  1:1105800/140‑1 (MQ=255)
                gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                            <  2:961335/140‑1 (MQ=255)
                gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCATCAGCTTCTCGGCCAGct                                                                                                                            <  1:2415839/140‑1 (MQ=255)
                  gCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctct                                                                                                                          >  2:1070964/1‑140 (MQ=255)
                  gCTCCACGGCAGAAAACATGTCCAGCGTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATAGCCCGCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctct                                                                                                                          <  1:698905/140‑1 (MQ=255)
                   cTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctc                                                                                                                         >  2:266132/1‑140 (MQ=255)
                   cTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctc                                                                                                                         >  1:1008180/1‑140 (MQ=255)
                     ccACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctctc                                                                                                                       <  2:298003/140‑1 (MQ=255)
                      ctcggcggAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGCCAGctcccgct                                                                                                                      <  2:1205147/138‑6 (MQ=255)
                        cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                    <  2:1635525/140‑1 (MQ=255)
                        cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                    >  1:1269634/1‑140 (MQ=255)
                        cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                    <  2:217686/140‑1 (MQ=255)
                        cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                    >  1:1808203/1‑140 (MQ=255)
                        cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                    <  2:891957/140‑1 (MQ=255)
                            aGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAAGGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAAGTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAgg                                                                                                                <  2:2236061/140‑1 (MQ=255)
                                  cATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCt                                                                                                          <  2:1481382/140‑1 (MQ=255)
                                     gTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCg                                                                                                       <  2:1008180/140‑1 (MQ=255)
                                        cAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAgt                                                                                                    >  1:2245631/1‑140 (MQ=255)
                                           cTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGtgtg                                                                                                 >  2:2311140/1‑140 (MQ=255)
                                               tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                             <  2:1336329/140‑1 (MQ=255)
                                               tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                             <  1:2118609/140‑1 (MQ=255)
                                               tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                             <  2:1590603/140‑1 (MQ=255)
                                                           cccTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCt                                                                                 >  1:2277541/1‑140 (MQ=255)
                                                                tCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGaa                                                                            >  1:2081827/1‑140 (MQ=255)
                                                                tCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGaa                                                                            >  1:1800483/1‑140 (MQ=255)
                                                                   cTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAAttt                                                                         >  1:1811270/1‑140 (MQ=255)
                                                                        tGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCtttt                                                                    >  1:589286/1‑140 (MQ=255)
                                                                           cGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAgg                                                                                                                >  1:1466645/1‑93 (MQ=255)
                                                                             tACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAg                                                               >  1:2036642/1‑140 (MQ=255)
                                                                             tACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAg                                                               >  1:1566816/1‑140 (MQ=255)
                                                                                  atatCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGttttt                                                          >  1:763274/1‑140 (MQ=255)
                                                                                    atCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctc                                                                                                                           >  2:1360008/1‑73 (MQ=255)
                                                                                    atCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctc                                                                                                                           <  1:1360008/73‑1 (MQ=255)
                                                                                     tCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGAt                                                       >  2:951733/1‑140 (MQ=255)
                                                                                     tCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGAt                                                       >  2:1369303/1‑140 (MQ=255)
                                                                                          cAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAAtt                                                  >  2:518340/1‑140 (MQ=255)
                                                                                             ccAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGc                                               >  1:1392414/1‑140 (MQ=255)
                                                                                                gAAGCTGCTGATAGGCTGAATTCCCTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGCACCTTCGAGTGTGGCAGCAGATTACGGGTGAGAATTTGTTTTTTTATTTTTGGATAAATTTGCTga                                            <  2:1503827/140‑1 (MQ=255)
                                                                                                       ctgATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTTCTGATAGatat                                     >  1:1271684/1‑140 (MQ=255)
                                                                                                         gagaGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  2:1694214/137‑1 (MQ=255)
                                                                                                         gacaGGCTGAATTCCCGGAGTTGACGCTGACCTTCAGCTTCTCGGCCAGCTCTCCCTTGGAGGGGACCGTCGAGGGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  2:1445558/137‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACTCTTACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGTACCTTCGAGTGTGGCAGAAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  2:2029410/140‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  2:1846502/140‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  2:941325/140‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  2:2277529/140‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  1:888514/140‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  1:2253977/140‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCGGATAGATATaa                                   <  2:745751/140‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTGTTTGATAAATTTGCTGATAGAtatac                                   <  2:1017572/140‑2 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCGTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  2:2019636/140‑1 (MQ=255)
                                                                                                         gATAGGCTGAATTCACTGAGTTTACGCTGAACTTCATCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTTTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                   <  1:926920/140‑1 (MQ=255)
                                                                                                             ggCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCgg                               <  2:147433/140‑1 (MQ=255)
                                                                                                              gCTGACTTCACTGAGTTTACGCGGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              <  2:1174001/140‑1 (MQ=255)
                                                                                                              gCTGAATTCACTGCGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              <  1:1469610/140‑1 (MQ=255)
                                                                                                              gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              <  1:1070964/140‑1 (MQ=255)
                                                                                                              gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              <  1:430594/140‑1 (MQ=255)
                                                                                                              gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              <  1:518340/140‑1 (MQ=255)
                                                                                                              gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              <  1:2311140/140‑1 (MQ=255)
                                                                                                              gCTGAATTCAATGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              <  2:1374339/140‑1 (MQ=255)
                                                                                                              gCTGAAGTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              <  1:1904574/140‑1 (MQ=255)
                                                                                                                        gtGAGTTTACTCTTAGCTTGAGGTTATCGTCCTGTTTTGTTTTGGTGGGGAGCTTCGATTGTGGCAGAATATTACTGCTGTTAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGTTAGTTATATTCGGTTTGTTTTGAg                    <  2:2174474/139‑1 (MQ=255)
                                                                                                                        cTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAg                    <  2:850278/140‑1 (MQ=255)
                                                                                                                        cTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAg                    <  2:1392414/140‑1 (MQ=255)
                                                                                                                             tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc               <  1:312405/140‑1 (MQ=255)
                                                                                                                             tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc               >  1:1149608/1‑140 (MQ=255)
                                                                                                                             tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc               <  1:448435/140‑1 (MQ=255)
                                                                                                                             tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc               <  1:884682/140‑1 (MQ=255)
                                                                                                                             tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc               >  1:1420899/1‑140 (MQ=255)
                                                                                                                             tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc               <  1:2014945/140‑1 (MQ=255)
                                                                                                                                tcGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg            <  2:1803053/139‑1 (MQ=255)
                                                                                                                                ccGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTGGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg            <  2:2133966/139‑1 (MQ=255)
                                                                                                                                aCGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg            <  2:1292592/140‑1 (MQ=255)
                                                                                                                                 cGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                              >  2:1737763/1‑121 (MQ=255)
                                                                                                                                 cGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAgt           <  2:633324/140‑1 (MQ=255)
                                                                                                                                    tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta        <  2:397235/140‑2 (MQ=255)
                                                                                                                                    tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta        <  2:621177/140‑2 (MQ=255)
                                                                                                                                    tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta        <  2:100111/140‑2 (MQ=255)
                                                                                                                                     gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa       >  1:1690238/1‑140 (MQ=255)
                                                                                                                                     gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa       <  1:259773/140‑1 (MQ=255)
                                                                                                                                     gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa       >  1:2326792/1‑140 (MQ=255)
                                                                                                                                     gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa       >  2:1832870/1‑140 (MQ=255)
                                                                                                                                      aCCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGaa      >  2:419511/1‑140 (MQ=255)
                                                                                                                                      aCCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGaa      >  2:409130/1‑140 (MQ=255)
                                                                                                                                          tCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAAtgtg  <  2:1962011/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GCAGTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTG  >  NZ_CP034943/438449‑438726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: