Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,611,711 A→G 5.4% intergenic (‑330/+4) alaS ← / ← EO946_RS13520 alanine‑‑tRNA ligase/AI‑2E family transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,611,7110AG 5.4% 470.4 / 11.6 147intergenic (‑330/+4)alaS/EO946_RS13520alanine‑‑tRNA ligase/AI‑2E family transporter
Reads supporting (aligned to +/- strand):  ref base A (82/56);  new base G (4/4);  total (87/60)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01

Only 100 of 155 total aligned reads displayed.

GATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAATGACGACAGG  >  NZ_CP034943/2611573‑2611849
                                                                                                                                          |                                                                                                                                          
gATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                           <  2:350396/140‑1 (MQ=255)
gATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                           <  2:1905372/140‑1 (MQ=255)
gATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                           <  2:1441516/140‑1 (MQ=255)
gATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                           <  1:1862832/140‑1 (MQ=255)
gATTAGCTTCCTCCGATCTTCTGAGAAAGCTCCTGTCAGCTTCCGGAGCTTCTCACTTTCACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCaa                                                                                                                                           <  1:1056679/140‑1 (MQ=255)
     gCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCa                                                                                                                                      <  2:90335/140‑1 (MQ=255)
     gCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCa                                                                                                                                      <  1:2084120/140‑1 (MQ=255)
        tcctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATg                                                                                                                                   <  2:2118034/140‑1 (MQ=255)
          ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACACGCTGCACGCCAATGTCAATGCt                                                                                                                                 >  2:1471129/1‑140 (MQ=255)
          ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                                 <  2:387667/140‑1 (MQ=255)
          ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                                 <  1:1626910/140‑1 (MQ=255)
          ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                                 <  2:2309972/140‑1 (MQ=255)
          ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                                 >  2:2176714/1‑140 (MQ=255)
          ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCt                                                                                                                                 >  2:1777881/1‑140 (MQ=255)
          ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAAGGCt                                                                                                                                 <  1:1447670/140‑1 (MQ=255)
          ctccGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCACATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAACCTGCACGCCAATGTCAATGCt                                                                                                                                 >  2:316870/1‑140 (MQ=255)
                               ccTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTaaaaaag                                                                                                            >  2:416342/1‑139 (MQ=255)
                                 tttCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATgg                                                                                                          <  2:1557713/140‑1 (MQ=255)
                                          aCGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                                 >  1:1017227/1‑140 (MQ=255)
                                          aCGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                                 <  2:1705953/140‑1 (MQ=255)
                                          aCGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                                 >  1:1109343/1‑140 (MQ=255)
                                                cTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACtttt                                                                                           >  2:302353/1‑140 (MQ=255)
                                                        tttAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAATCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGc                                                                                   >  2:1100345/1‑140 (MQ=255)
                                                        tttAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGc                                                                                   >  2:511856/1‑140 (MQ=255)
                                                          tAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCgg                                                                                 >  1:336193/1‑140 (MQ=255)
                                                           cagaGGGCGTCGGCGTACTCAGATCCGTCACTCTAGATTCTTATGTATTCTTCGTGATTTTATAGAGAATCTGGACGCCAATGTCATTGCGCCGTCCTCATCCGTATAAAATGGATCATCATCACTTTTAAAACAGCGGt                                                                                <  2:2188639/137‑1 (MQ=255)
                                                                ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATTTATTCGACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCTCAg                                                                           >  1:190764/1‑140 (MQ=255)
                                                                ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                                 <  2:11956/118‑1 (MQ=255)
                                                                ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                                 >  1:11956/1‑118 (MQ=255)
                                                                ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGatcatcatc                                                                                                 >  1:389445/1‑118 (MQ=255)
                                                                ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAg                                                                           >  1:2216667/1‑140 (MQ=255)
                                                                ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAg                                                                           >  2:1483270/1‑140 (MQ=255)
                                                                ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAg                                                                           >  1:1373512/1‑140 (MQ=255)
                                                                ggCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAg                                                                           >  1:1028521/1‑140 (MQ=255)
                                                                  cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACACGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                         >  2:763792/1‑140 (MQ=255)
                                                                  cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                         <  2:2151345/140‑1 (MQ=255)
                                                                  cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                         >  1:105012/1‑140 (MQ=255)
                                                                  cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                         <  2:577971/140‑1 (MQ=255)
                                                                  cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGa                                                                         >  2:499552/1‑140 (MQ=255)
                                                                  cTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGa                                                                         >  2:499582/1‑140 (MQ=255)
                                                                  cTTCGGCGTACTCAGATACGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGa                                                                         <  2:2120481/140‑1 (MQ=255)
                                                                   ttCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATGATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGaa                                                                        <  2:261992/140‑1 (MQ=255)
                                                                      ggCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGCAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAg                                                                     >  2:184227/1‑140 (MQ=255)
                                                                            cTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGaa                                                               >  2:168887/1‑140 (MQ=255)
                                                                            cTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGaa                                                               >  2:2053933/1‑140 (MQ=255)
                                                                                 atgcGTCACTCGAGATTCGTATGTATTCTACGAGAACTTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAACATGGATCCTCATCCCTTTTAAAACAGCGGTCGCAGGAACAGCCCGAATCAtt                                                          >  2:139273/4‑140 (MQ=255)
                                                                                 aTCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCAtt                                                          >  2:2309892/1‑140 (MQ=255)
                                                                                 aTCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCAtt                                                          >  2:2287101/1‑140 (MQ=255)
                                                                                     gTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCaa                                                      <  1:1136677/140‑1 (MQ=255)
                                                                                      tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCCATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                                     >  2:600611/1‑140 (MQ=255)
                                                                                      tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                                     >  2:2048254/1‑140 (MQ=255)
                                                                                      tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                                     >  2:211054/1‑140 (MQ=255)
                                                                                      tCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAAc                                                     >  2:2186329/1‑140 (MQ=255)
                                                                                        aCTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGa                                                   <  1:2053933/140‑1 (MQ=255)
                                                                                        aCTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGa                                                   <  1:168887/140‑1 (MQ=255)
                                                                                          tCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGAtt                                                 <  2:1713820/140‑1 (MQ=255)
                                                                                               aTTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                            <  1:2007531/140‑1 (MQ=255)
                                                                                               aTTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                            <  1:1708737/140‑1 (MQ=255)
                                                                                               aTTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                            <  2:2418653/140‑1 (MQ=255)
                                                                                                  cGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACCGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAg                                         <  2:1333461/140‑1 (MQ=255)
                                                                                                  cGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAg                                         <  2:110176/140‑1 (MQ=255)
                                                                                                             tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGa                                                                <  2:73189/106‑1 (MQ=255)
                                                                                                             tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGa                                                                >  1:73189/1‑106 (MQ=255)
                                                                                                             tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGa                                                                <  2:1194195/106‑1 (MQ=255)
                                                                                                             tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCgg                              >  1:2120140/1‑140 (MQ=255)
                                                                                                             tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCgg                              >  1:2333561/1‑140 (MQ=255)
                                                                                                             tACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGAGTCCCGCAAGCTCTCCTCCgg                              >  2:2272359/1‑140 (MQ=255)
                                                                                                              aCGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGc                             <  2:2333561/140‑1 (MQ=255)
                                                                                                              aCGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGc                             <  2:2120140/140‑1 (MQ=255)
                                                                                                               cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                            <  2:2175520/124‑1 (MQ=255)
                                                                                                               cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGc                                            >  1:2175520/1‑124 (MQ=255)
                                                                                                               cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCa                            >  1:917471/1‑140 (MQ=255)
                                                                                                               cGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCa                            >  1:2385989/1‑140 (MQ=255)
                                                                                                                 agaAATTCTAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGAGTCCCGCAAGCTCTCCTCCGGCAAg                          <  2:826864/140‑1 (MQ=255)
                                                                                                                 agaAATTATAGACCAGCGGCACGCCAATGTCAATGCTCCGTCCTCCTCCGTAAAAAATGGATCATCATCACTTTTAAACCAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                          <  1:416342/140‑1 (MQ=255)
                                                                                                                 agaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                          <  2:171146/140‑1 (MQ=255)
                                                                                                                 agaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                          <  1:639241/140‑1 (MQ=255)
                                                                                                                 agaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                          <  2:1268302/140‑1 (MQ=255)
                                                                                                                 agaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                          <  1:933622/140‑1 (MQ=255)
                                                                                                                 agaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAg                          <  1:766207/140‑1 (MQ=255)
                                                                                                                 agaAATTATAGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGAACAGCCAGAATCATCCCGACGATACCCGCAAGCTCCCCTCCGGCAAg                          <  1:499552/140‑1 (MQ=255)
                                                                                                                 agaAATTATAGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGAACAGCCAGAATCATCCCGACGATACCCGCAAGCTCCCCTCCGGCAAg                          <  1:499582/140‑1 (MQ=255)
                                                                                                                  gaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGc                         <  2:602836/140‑1 (MQ=255)
                                                                                                                  gaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGc                         >  1:2034189/1‑140 (MQ=255)
                                                                                                                  gaAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGc                         <  2:1990709/140‑1 (MQ=255)
                                                                                                                  caaaTTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGc                         <  2:1297700/139‑1 (MQ=255)
                                                                                                                       tataGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGc                    <  1:2176714/140‑1 (MQ=255)
                                                                                                                       tataGACAAGCGGCACGCCGATGTCAATGCTCCGTCCTCATCCGCAAAAAATGGATCATCATTACTTTTAAAACAGCCGTCGCAGGAACAGCCAGAATCATCCCGACGATACCCGCAAGCTCCCCTCCGGCAAGGAGTGc                    <  1:235341/140‑1 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCTTCCTCAGCCGTAAAACATGGATCTTCATCAGTTGGACAACAGCGGTCGCAGGAACAGCCTGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCCAGCCGTGCAAGCATAAt           >  1:2347536/1‑140 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCATGCAGTGCAAGCATAAt           >  1:1363753/1‑140 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGc                    <  2:678049/131‑1 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGc                    >  1:678049/1‑131 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt           >  1:1212006/1‑140 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt           >  2:587218/1‑140 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt           >  1:2411394/1‑140 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt           >  1:1564331/1‑140 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt           >  1:844014/1‑140 (MQ=255)
                                                                                                                                gCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAAt           >  2:1559026/1‑140 (MQ=255)
                                                                                                                                         cAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAATGACGACAgg  >  2:406570/1‑140 (MQ=255)
                                                                                                                                         cAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAATGACGACAgg  >  2:1972358/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
GATTAGCTTCCTCCGATCTTCTGATAAAGCTCCTTTCAGCTTACGGAGCTTCTCTCTTTAACAGGGCTTCGGCGTACTCAGATCCGTCACTCGAGATTCGTATGTATTCTACGAGAAATTATAGACAAGCTGCACGCCAATGTCAATGCTCCGTCCTCATCCGTAAAAAATGGATCATCATCACTTTTAAAACAGCGGTCGCAGGAACAGCCAGAATCATTCCAACGATTCCCGCAAGCTCTCCTCCGGCAAGCAGTGCAAGCATAATGACGACAGG  >  NZ_CP034943/2611573‑2611849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: