Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,706,418 G→T 6.4% intergenic (‑539/‑278) ilvB ← / → EO946_RS13980 acetolactate synthase large subunit/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,706,4180GT 6.4% 371.1 / 2.9 131intergenic (‑539/‑278)ilvB/EO946_RS13980acetolactate synthase large subunit/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (24/95);  new base T (10/2);  total (34/97)
Fisher's exact test for biased strand distribution p-value = 2.01e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

TTCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAATATCATATAAA  >  NZ_CP034943/2706279‑2706552
                                                                                                                                           |                                                                                                                                      
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAATCTTGCGTTTATCTTTTGTTTAACTAATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAg                                                                                                                                        >  1:1708813/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAg                                                                                                                                        >  2:918393/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAg                                                                                                                                        >  2:1321924/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAg                                                                                                                                        >  2:2262725/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATATTTTGTTTTACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAg                                                                                                                                        >  1:2160586/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGATTGTGAAGCTTGCGTTTATATTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTACTATTATTAg                                                                                                                                        >  2:1825792/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCAGTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAg                                                                                                                                        >  1:1050113/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCAAATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAg                                                                                                                                        >  2:1185289/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAAACATATTGCGATCATTTTTGGGTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCCGTGTCAACAGCATTTTTTAAAATTATTAg                                                                                                                                        >  1:1016938/1‑140 (MQ=255)
ttCAAGGAAGTCGTTTGTTTTTTCACTTTTAAGAATCATATTGCGATCATTTTTGCGTGTGAAGCTTGCGTTGATCTTTTGTTGGAATCATATTAAGCCCCTTTGGACGCAGTGTCAACAGCCTTTTTTAAAATTATTAg                                                                                                                                        >  1:328843/1‑140 (MQ=255)
     ggAAGTCGTTTGTTTTTTCACTTTTCAGAATGCTATTGCGATCATTTTTGCTTGTGACGATTGGGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGAGGCAGTGTCAAGAGCATTTTTTAAAATTATTAGACAAt                                                                                                                                   >  2:747752/1‑140 (MQ=255)
          tCGTTTGTTTTTTCACTTTTGAGAATCATATTGCGATCATTTTTTCTTGTGAAGCTTGCGTTTATCTTTTTTTGTACTCATATTTCGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTATACAAttttta                                                                                                                              >  1:886097/1‑138 (MQ=255)
          tCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGa                                                                                                                              >  2:1131844/1‑140 (MQ=255)
          tCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCAATTTTTCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTAAGCCGCTTTGGACGCAGTGTCAACAGCAATTTTTAAAATTATTATACAAttttta                                                                                                                              >  1:886280/1‑138 (MQ=255)
          tCGTTTGTTTTTTCACTTTTCAGAATAATATTGCGCTCCTTTTTGCTTGTGAAGCTTGCGTTTATCTTTTTTTTAACTAATATTAACCCCCGTTTGAAGCAGTGTCAACAGCAATTTTTAAAATTATTATAAAAatttta                                                                                                                              >  1:288728/1‑138 (MQ=255)
          tCGTTTGTTTTTTCACTTTTAAGAAAAAAATTTCGATAATATTTTCTTGTGAACCATTCGTTTATATTTTGTTGAACTCAAATTACGACGCTTTTGACGCAGTGTCAACAGCATTTTTTATAATTATTATACAAtttttt                                                                                                                              >  1:1609577/1‑138 (MQ=255)
          tCGTTTGTTTTTTAAATTTTCAGAATAATATTGCGATAATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTATACAAttttta                                                                                                                              >  1:927877/1‑138 (MQ=255)
          tCGTGTGTTTTTTGACTTTTCACAATCATATATCGATAATTTTTGCTTGTGAAGCTTGCGGTTATCATTTGTTTAACTCATAATAATCCGCTGTGGACTCAGTGGCAACAGCAGTTTTTAAAATTATTATACATTTTTGa                                                                                                                              >  2:305242/1‑140 (MQ=255)
              ttttattttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaa                                                                                                                          <  2:1413822/135‑1 (MQ=255)
              ttgtttTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaa                                                                                                                          <  2:909271/140‑1 (MQ=255)
              ttgtttTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaa                                                                                                                          <  2:1084666/140‑1 (MQ=255)
              ttgtttTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaa                                                                                                                          <  1:2330504/140‑1 (MQ=255)
              ttgtttTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaa                                                                                                                          <  1:2254258/140‑1 (MQ=255)
              ttgtttTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaa                                                                                                                          <  2:362083/140‑1 (MQ=255)
                tttttttAACTTTTCAGAATCATATTGCGATGATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatc                                                                                                                        <  1:1482024/139‑1 (MQ=255)
                gtttTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatc                                                                                                                        <  2:951708/140‑1 (MQ=255)
                gtttTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatc                                                                                                                        <  1:1815898/140‑1 (MQ=255)
                gtttGTTCACTGTTCCGCATCATATTGCGATCCTTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatc                                                                                                                        <  1:1896093/140‑1 (MQ=255)
                 ttttttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatca                                                                                                                       <  1:1900665/140‑1 (MQ=255)
                   ttttcagttttcagAATCATATTGCGATGATTTTTGCTTTTGAAGCTTGCGTTTATCTTTTGTTGCACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatcaat                                                                                                                     <  2:2133504/140‑1 (MQ=255)
                   ttttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatcaat                                                                                                                     <  1:133983/140‑1 (MQ=255)
                   ttttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatcaat                                                                                                                     <  2:1295219/140‑1 (MQ=255)
                   ttttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatcaat                                                                                                                     <  2:1163361/140‑1 (MQ=255)
                   ttttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatcaat                                                                                                                     <  1:1778826/140‑1 (MQ=255)
                   ttttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatcaat                                                                                                                     <  1:2081447/140‑1 (MQ=255)
                   tttaCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTaatcaat                                                                                                                     <  1:1346122/140‑1 (MQ=255)
                     ttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCt                                                                                                                   <  2:2217407/140‑1 (MQ=255)
                     ttCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCt                                                                                                                   <  2:521845/140‑1 (MQ=255)
                        aCTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGCCGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCtata                                                                                                                <  2:1094824/140‑1 (MQ=255)
                        aCTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCtata                                                                                                                <  1:1526980/140‑1 (MQ=255)
                        aCTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCtata                                                                                                                <  2:36632/140‑1 (MQ=255)
                        aCTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCtata                                                                                                                <  2:705377/140‑1 (MQ=255)
                        aCTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCtata                                                                                                                <  2:1328185/140‑1 (MQ=255)
                        aCTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCtata                                                                                                                <  1:860403/140‑1 (MQ=255)
                          ttttCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTTCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATAtt                                                                                                              <  1:1241075/140‑1 (MQ=255)
                                   tCATATTGCGATCATTTTTTCTTGTGAAGCTTGCGTTTATATTTTGTTGAACTCATATTACGCAGCTTTTGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCt                                                                                                     <  1:696734/140‑1 (MQ=255)
                                   tCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCt                                                                                                     <  1:2401117/140‑1 (MQ=255)
                                   tCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCt                                                                                                     <  1:696679/140‑1 (MQ=255)
                                      tatTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTc                                                                                                  >  1:707482/1‑140 (MQ=255)
                                      tatTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTc                                                                                                  >  1:719830/1‑140 (MQ=255)
                                           cGATCATTTTTGCTTGTGAAGCTTGCGTGTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCAATTTTTAAAATTATTAGACAATTTTGATTAATCCATCTATATTGTATACGCTTTCattat                                                                                             >  2:1032284/1‑140 (MQ=255)
                                            gATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCattatt                                                                                            <  1:152020/140‑1 (MQ=255)
                                            gATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCattatt                                                                                            <  1:1151896/140‑1 (MQ=255)
                                            gATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCattatt                                                                                            <  1:1180470/140‑1 (MQ=255)
                                            gATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCattatt                                                                                            <  1:2332378/140‑1 (MQ=255)
                                             aTCATATTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCAGATTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTg                                                                                           <  1:2244934/140‑1 (MQ=255)
                                                                 ttGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAAt                                                                       <  1:2387803/140‑1 (MQ=255)
                                                                                         atatTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTc                                               >  1:1972792/1‑140 (MQ=255)
                                                                                             tACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTa                                           <  2:2320878/140‑1 (MQ=255)
                                                                                             tACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTa                                           <  2:2138437/140‑1 (MQ=255)
                                                                                               cgccgcTTTGGACGCATTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAAt                                         <  1:301998/140‑1 (MQ=255)
                                                                                               cgccgcTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAAt                                         <  2:2160586/140‑1 (MQ=255)
                                                                                               cgccgcTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAAt                                         <  1:605553/140‑1 (MQ=255)
                                                                                               cgccgcTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAAt                                         <  2:7048/140‑1 (MQ=255)
                                                                                               cgccgcTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGAAAATTTTGATTAATCAATATATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAAt                                         <  2:2081925/140‑1 (MQ=255)
                                                                                                   gcTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTAt                                     >  1:1835124/1‑140 (MQ=255)
                                                                                                    cTTTGGACTCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATATATATTGTATACGCTTTCATTTTTGATGCATAATGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATc                                    <  1:404508/140‑1 (MQ=255)
                                                                                                    cTTTGGACGCAGTGTCAACATCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATc                                    <  1:1321924/140‑1 (MQ=255)
                                                                                                    cTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACTATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATc                                    <  1:371306/140‑1 (MQ=255)
                                                                                                    cTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATc                                    <  1:2168364/140‑1 (MQ=255)
                                                                                                    cTTTGGAAGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGTAATAGCAAATGTTAAAAGATTTTATAATTTTTTCATTAATCTATc                                    >  1:1642359/1‑140 (MQ=255)
                                                                                                     tttGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCa                                   >  1:523595/1‑140 (MQ=255)
                                                                                                         gACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTTTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGa                               <  1:1396144/140‑1 (MQ=255)
                                                                                                         gACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGa                               <  2:1133156/140‑1 (MQ=255)
                                                                                                              aGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATacac                          >  1:1432933/1‑140 (MQ=255)
                                                                                                              aGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATacac                          >  1:1846538/1‑140 (MQ=255)
                                                                                                              aGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATacac                          >  2:1775579/1‑140 (MQ=255)
                                                                                                              aGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATacac                          >  1:1382634/1‑140 (MQ=255)
                                                                                                              aGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATacac                          >  2:501342/1‑140 (MQ=255)
                                                                                                              aGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTAATTAATCTATCAAGGATacac                          >  2:2243309/1‑140 (MQ=255)
                                                                                                                 ttCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAg                       <  2:1643324/139‑1 (MQ=255)
                                                                                                                 gtCAACAGCATTTTTTATAATTATTAGACAATTTTGATTAATCAATATATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAg                       <  1:403642/140‑1 (MQ=255)
                                                                                                                 gtCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAg                       <  1:2156468/140‑1 (MQ=255)
                                                                                                                   cAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATCATTTTTTCATTAATCTATCAAGGATACACCAGaa                     <  2:1177365/140‑1 (MQ=255)
                                                                                                                   cAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGaa                     <  2:1360211/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTATACAATTTTGATTATTCATTCTTTATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTATTCTATCAATGATACACCAGAATTTTTCAat            <  2:1598053/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTATAAAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:1708813/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTTTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:1345755/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            >  1:1031020/1‑140 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:28625/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:279208/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:2117756/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:1538298/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:1511261/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            >  1:1552736/1‑140 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:1016938/140‑1 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            >  1:420221/1‑140 (MQ=255)
                                                                                                                            tttttAAAATTATTAGACAATTTTGATTAATCAATATATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAat            <  2:1830233/140‑1 (MQ=255)
                                                                                                                                    attattAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAATATCAtata    <  2:1264948/140‑1 (MQ=255)
                                                                                                                                      tattaGACAATTTTGATTAATCAATCTCTATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAATATCATATaaa  <  2:1032456/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                      
TTCAAGGAAGTCGTTTGTTTTTTCACTTTTCAGAATCATATTGCGATCATTTTTGCTTGTGAAGCTTGCGTTTATCTTTTGTTGAACTCATATTACGCCGCTTTGGACGCAGTGTCAACAGCATTTTTTAAAATTATTAGACAATTTTGATTAATCAATCTATATTGTATACGCTTTCATTATTGATGCATAAGGAATCGCAAATGTTAAAAGTTTTTATAATTTTTTCATTAATCTATCAAGGATACACCAGAATTTTTCAATATCATATAAA  >  NZ_CP034943/2706279‑2706552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: