BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,711,930	G→T	7.9%	T127T (ACC→ACA)	EO946_RS14015 ←	ribonuclease PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,711,930	0	G	T	7.9%	278.5 / 9.6	95	T127T (ACC→ACA)	EO946_RS14015	ribonuclease PH
Reads supporting (aligned to +/- strand): ref base G (49/38); new base T (2/6); total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

ACAAAATTCCCTGTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCC  >  NZ_CP034943/2711791‑2712069

aCAAAATTCCCTGTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCg                                                                                                                                             >  2:1493500/1‑140 (MQ=255)

aCAAAATTCCCTGTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCg                                                                                                                                             >  2:2195676/1‑140 (MQ=255)

aCAAAATTCCCTGTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCg                                                                                                                                             >  2:297372/1‑140 (MQ=255)

aCAAAATTCCCTGTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCg                                                                                                                                             >  2:90930/1‑140 (MQ=255)

aCAAAATTCCCTGTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCg                                                                                                                                             >  2:128145/1‑140 (MQ=255)

aCAAAATTCCCTGTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGGCTTGATCGTTCCAGCTTTGATCAGCTTGCCATTAGCAATCGCCATAGCAAGAAATGGACGTGTAATGGAAGCg                                                                                                                                             >  2:1436597/1‑140 (MQ=255)

            gTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCg                                                                                                                                 >  2:1667706/1‑140 (MQ=255)

            gTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCg                                                                                                                                 >  2:1011637/1‑140 (MQ=255)

            gTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCg                                                                                                                                 >  2:1012830/1‑140 (MQ=255)

            gTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCg                                                                                                                                 >  2:1334174/1‑140 (MQ=255)

               ccTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCACTCGCCATAGCACGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCa                                                                                                                              >  2:1255100/1‑140 (MQ=255)

               ccTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCa                                                                                                                              >  1:2287181/1‑140 (MQ=255)

                cTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                             <  2:1754575/140‑1 (MQ=255)

                cTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                             <  2:391538/140‑1 (MQ=255)

                cTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                             <  2:695595/140‑1 (MQ=255)

                  tATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCa                                                                                                                           >  1:1297443/1‑140 (MQ=255)

                  tATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCa                                                                                                                           >  1:2164020/1‑140 (MQ=255)

                       aTTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATGTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGGTCTTGTCCCGCCATCAGCCTg                                                                                                                      >  2:134253/1‑140 (MQ=255)

                         tCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGaa                                                                                                                    >  1:2083803/1‑140 (MQ=255)

                          ccGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAAt                                                                                                                   <  1:491431/140‑1 (MQ=255)

                           cGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATg                                                                                                                  <  2:1175763/140‑1 (MQ=255)

                           cGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATg                                                                                                                  <  2:1739150/140‑1 (MQ=255)

                             aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                >  1:131872/1‑140 (MQ=255)

                             aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                >  1:1351187/1‑140 (MQ=255)

                             aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                >  1:1240133/1‑140 (MQ=255)

                                 agaTCGCAGCAAGAAAATCATTGATCGGATTTGTATTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATca                                                                                                            <  2:2076801/140‑1 (MQ=255)

                                 agaTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATca                                                                                                            <  2:457492/140‑1 (MQ=255)

                                   aTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATcaca                                                                                                          >  1:985981/1‑140 (MQ=255)

                                   aTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATcaca                                                                                                          >  1:905031/1‑140 (MQ=255)

                                   aTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATcaca                                                                                                          >  1:1531556/1‑140 (MQ=255)

                                     cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                        <  1:1256019/140‑1 (MQ=255)

                                     cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                        <  1:1986173/140‑1 (MQ=255)

                                     cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                        >  1:1229261/1‑140 (MQ=255)

                                                  tCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGt                                                                                           >  1:616837/1‑140 (MQ=255)

                                                    aGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCCTCAGCCTGAATGACATCACAGTCAATCCAAATCGTcc                                                                                         >  1:1594319/1‑140 (MQ=255)

                                                      tGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATACAAATAGTCCGt                                                                                       >  2:1859546/1‑140 (MQ=255)

                                                        aTCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                             <  2:401572/100‑1 (MQ=255)

                                                        aTCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                             >  1:401572/1‑100 (MQ=255)

                                                              tttGTGTTGATCGTTCCCGCTTTGATCAGTTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCTTCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAg                                                                               <  1:465661/140‑1 (MQ=255)

                                                                   cTTGATCGTTCCCGCTTTGATCAGCGTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCtttt                                                                          <  2:456795/140‑1 (MQ=255)

                                                                     tGATTGTTGCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCTAGAAATGCGCCTGTAATGGAATCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                        <  2:1846151/140‑2 (MQ=255)

                                                                     tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                        <  2:1846058/140‑2 (MQ=255)

                                                                     tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                        <  2:1846160/140‑2 (MQ=255)

                                                                     tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                        <  2:713573/140‑2 (MQ=255)

                                                                     tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTCCACCGAGCTTTTcc                                                                        <  2:1048516/140‑2 (MQ=255)

                                                                     tGATTGTTCCTGCTATGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGGTCACCGAGCTTTTcc                                                                        <  2:594079/140‑2 (MQ=255)

                                                                     tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                        <  2:919381/140‑1 (MQ=255)

                                                                     tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                        <  1:1171471/140‑1 (MQ=255)

                                                                     tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                        <  1:1369828/140‑1 (MQ=255)

                                                                     tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                        <  1:2212735/140‑1 (MQ=255)

                                                                     tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                        <  2:1019725/140‑1 (MQ=255)

                                                                     tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                        <  1:1304899/140‑1 (MQ=255)

                                                                     tGATCGGTCCCGCTTTGATCAGCGTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                        <  1:1236237/140‑1 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                        >  1:1476010/1‑106 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                        <  2:1476010/106‑1 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                      >  2:19041/1‑140 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                      >  1:25282/1‑140 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                      >  1:32562/1‑140 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                      >  1:544804/1‑140 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                      >  1:924741/1‑140 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                      >  1:602384/1‑140 (MQ=255)

                                                                       aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                      >  1:648590/1‑140 (MQ=255)

                                                                           ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                  >  2:1981795/1‑140 (MQ=255)

                                                                           ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                  >  2:378765/1‑140 (MQ=255)

                                                                           ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                  >  2:1729200/1‑140 (MQ=255)

                                                                            tCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTa                                                                 >  1:385281/1‑140 (MQ=255)

                                                                              ccGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCCCAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACg                                                               <  1:2138296/140‑1 (MQ=255)

                                                                              ccGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACg                                                               <  2:82035/140‑1 (MQ=255)

                                                                                gCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTGGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGAc                                                             >  2:1343626/1‑140 (MQ=255)

                                                                                           cTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                        >  1:180362/1‑118 (MQ=255)

                                                                                              gCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGGTCTTGTCCCGCCATCAGCCTGAATGACATCACTGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAgcgcgc                                               >  1:1148270/1‑140 (MQ=255)

                                                                                                      caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                       <  2:621992/140‑1 (MQ=255)

                                                                                                      caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                       <  2:218412/140‑1 (MQ=255)

                                                                                                      caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                       <  2:623331/140‑1 (MQ=255)

                                                                                                      caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                       <  2:1472179/140‑1 (MQ=255)

                                                                                                           gcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCg                                  >  2:107791/1‑140 (MQ=255)

                                                                                                           gcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCg                                  >  2:108002/1‑140 (MQ=255)

                                                                                                               tAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGa                              <  1:91348/140‑1 (MQ=255)

                                                                                                               tAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGGTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGa                              <  1:847122/140‑1 (MQ=255)

                                                                                                                    aGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAAtctc                         <  2:25282/140‑1 (MQ=255)

                                                                                                                    aGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAAtctc                         <  2:32562/140‑1 (MQ=255)

                                                                                                                                gTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCACTCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGcc             >  1:860321/1‑140 (MQ=255)

                                                                                                                                        aGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCCGGATCTACAACAGCTCGAAGCGCGCGGCCGATCAGGCGTTGGATCTCCATTGTTCTGCCGGAAAttt     >  2:1340154/1‑140 (MQ=255)

                                                                                                                                        aGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCCAGATCTACAACAGCTCGAAGCGCGCGGCCGATCAGGCGTTGGATCTCCATTGTTCTGCCGGAAAttt     >  2:1404803/1‑140 (MQ=255)

                                                                                                                                        aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                        >  1:1076378/1‑105 (MQ=255)

                                                                                                                                        aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                        >  2:2423967/1‑105 (MQ=255)

                                                                                                                                        aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                        <  1:2423967/105‑1 (MQ=255)

                                                                                                                                        aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                        <  2:1076378/105‑1 (MQ=255)

                                                                                                                                        aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt     >  2:2095039/1‑140 (MQ=255)

                                                                                                                                        aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt     >  2:1733208/1‑140 (MQ=255)

                                                                                                                                        aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt     >  2:1689537/1‑140 (MQ=255)

                                                                                                                                        aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt     >  2:1100898/1‑140 (MQ=255)

                                                                                                                                         gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGTCTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt    <  2:522978/140‑1 (MQ=255)

                                                                                                                                         gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt    <  1:1626697/140‑1 (MQ=255)

                                                                                                                                         gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt    <  2:523437/140‑1 (MQ=255)

                                                                                                                                         gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt    <  2:180039/140‑1 (MQ=255)

                                                                                                                                           ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc  <  2:2320622/140‑1 (MQ=255)

                                                                                                                                           ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc  <  2:614315/140‑1 (MQ=255)

                                                                                                                                           ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc  <  2:2079590/140‑1 (MQ=255)

                                                                                                                                           ggTTCGTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc  <  2:2320791/140‑1 (MQ=255)

ACAAAATTCCCTGTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCC  >  NZ_CP034943/2711791‑2712069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`