Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,711,942 G→T 7.4% G123G (GGC→GGA EO946_RS14015 ← ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,711,9420GT 7.4% 300.5 / 6.9 99G123G (GGC→GGAEO946_RS14015ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base G (48/43);  new base T (2/6);  total (50/49)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

GTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATT  >  NZ_CP034943/2711803‑2712081
                                                                                                                                           |                                                                                                                                           
gTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCg                                                                                                                                             >  2:1011637/1‑140 (MQ=255)
gTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCg                                                                                                                                             >  2:1012830/1‑140 (MQ=255)
gTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCg                                                                                                                                             >  2:1667706/1‑140 (MQ=255)
gTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCg                                                                                                                                             >  2:1334174/1‑140 (MQ=255)
   ccTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCa                                                                                                                                          >  1:2287181/1‑140 (MQ=255)
    cTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                                         <  2:391538/140‑1 (MQ=255)
    cTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                                         <  2:695595/140‑1 (MQ=255)
      tATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCa                                                                                                                                       >  1:1297443/1‑140 (MQ=255)
      tATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCa                                                                                                                                       >  1:2164020/1‑140 (MQ=255)
           aTTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATGTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGGTCTTGTCCCGCCATCAGCCTg                                                                                                                                  >  2:134253/1‑140 (MQ=255)
             tCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGaa                                                                                                                                >  1:2083803/1‑140 (MQ=255)
              ccGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAAt                                                                                                                               <  1:491431/140‑1 (MQ=255)
               cGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATg                                                                                                                              <  2:1739150/140‑1 (MQ=255)
               cGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATg                                                                                                                              <  2:1175763/140‑1 (MQ=255)
                 aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                            >  1:1351187/1‑140 (MQ=255)
                 aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                            >  1:131872/1‑140 (MQ=255)
                 aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                            >  1:1240133/1‑140 (MQ=255)
                     agaTCGCAGCAAGAAAATCATTGATCGGATTTGTATTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATca                                                                                                                        <  2:2076801/140‑1 (MQ=255)
                       aTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATcaca                                                                                                                      >  1:905031/1‑140 (MQ=255)
                       aTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATcaca                                                                                                                      >  1:985981/1‑140 (MQ=255)
                         cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                    <  1:1986173/140‑1 (MQ=255)
                         cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                    >  1:1229261/1‑140 (MQ=255)
                         cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                    <  1:1256019/140‑1 (MQ=255)
                                      tCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGt                                                                                                       >  1:616837/1‑140 (MQ=255)
                                        aGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCCTCAGCCTGAATGACATCACAGTCAATCCAAATCGTcc                                                                                                     >  1:1594319/1‑140 (MQ=255)
                                          tGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATACAAATAGTCCGt                                                                                                   >  2:1859546/1‑140 (MQ=255)
                                            aTCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                                         <  2:401572/100‑1 (MQ=255)
                                            aTCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCAt                                                                                                                                         >  1:401572/1‑100 (MQ=255)
                                                  tttGTGTTGATCGTTCCCGCTTTGATCAGTTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCTTCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAg                                                                                           <  1:465661/140‑1 (MQ=255)
                                                       cTTGATCGTTCCCGCTTTGATCAGCGTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCtttt                                                                                      <  2:456795/140‑1 (MQ=255)
                                                         tGATTGTTGCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCTAGAAATGCGCCTGTAATGGAATCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                                    <  2:1846151/140‑2 (MQ=255)
                                                         tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                                    <  2:1846058/140‑2 (MQ=255)
                                                         tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                                    <  2:1846160/140‑2 (MQ=255)
                                                         tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                                    <  2:713573/140‑2 (MQ=255)
                                                         tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTCCACCGAGCTTTTcc                                                                                    <  2:1048516/140‑2 (MQ=255)
                                                         tGATTGTTCCTGCTATGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGGTCACCGAGCTTTTcc                                                                                    <  2:594079/140‑2 (MQ=255)
                                                         tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    <  2:1019725/140‑1 (MQ=255)
                                                         tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    <  2:919381/140‑1 (MQ=255)
                                                         tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    <  1:2212735/140‑1 (MQ=255)
                                                         tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    <  1:1304899/140‑1 (MQ=255)
                                                         tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    <  1:1369828/140‑1 (MQ=255)
                                                         tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    <  1:1171471/140‑1 (MQ=255)
                                                         tGATCGGTCCCGCTTTGATCAGCGTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    <  1:1236237/140‑1 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                    >  1:1476010/1‑106 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                    <  2:1476010/106‑1 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                  >  1:544804/1‑140 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                  >  1:32562/1‑140 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                  >  1:25282/1‑140 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                  >  2:19041/1‑140 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                  >  1:602384/1‑140 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                  >  1:648590/1‑140 (MQ=255)
                                                           aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                  >  1:924741/1‑140 (MQ=255)
                                                               ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                              >  2:378765/1‑140 (MQ=255)
                                                               ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                              >  2:1981795/1‑140 (MQ=255)
                                                               ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                              >  2:1729200/1‑140 (MQ=255)
                                                                tCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTa                                                                             >  1:385281/1‑140 (MQ=255)
                                                                tCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTACTAAATCtc                                                                             >  1:675418/1‑139 (MQ=255)
                                                                  ccGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCCCAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACg                                                                           <  1:2138296/140‑1 (MQ=255)
                                                                  ccGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACg                                                                           <  2:82035/140‑1 (MQ=255)
                                                                    gCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTGGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGAc                                                                         >  2:1343626/1‑140 (MQ=255)
                                                                               cTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    >  1:180362/1‑118 (MQ=255)
                                                                               cTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                    <  2:180362/118‑1 (MQ=255)
                                                                                  gCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGGTCTTGTCCCGCCATCAGCCTGAATGACATCACTGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAgcgcgc                                                           >  1:1148270/1‑140 (MQ=255)
                                                                                          caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                                   <  2:1472179/140‑1 (MQ=255)
                                                                                          caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                                   <  2:623331/140‑1 (MQ=255)
                                                                                          caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                                   <  2:621992/140‑1 (MQ=255)
                                                                                               gcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCg                                              >  2:107791/1‑140 (MQ=255)
                                                                                               gcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCg                                              >  2:108002/1‑140 (MQ=255)
                                                                                                   tAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGGTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGa                                          <  1:847122/140‑1 (MQ=255)
                                                                                                        aGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAAtctc                                     <  2:32562/140‑1 (MQ=255)
                                                                                                        aGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAAtctc                                     <  2:25282/140‑1 (MQ=255)
                                                                                                             tGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTg                                >  1:1968957/1‑140 (MQ=255)
                                                                                                                    gTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCACTCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGcc                         >  1:860321/1‑140 (MQ=255)
                                                                                                                            aGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCCGGATCTACAACAGCTCGAAGCGCGCGGCCGATCAGGCGTTGGATCTCCATTGTTCTGCCGGAAAttt                 >  2:1340154/1‑140 (MQ=255)
                                                                                                                            aGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCCAGATCTACAACAGCTCGAAGCGCGCGGCCGATCAGGCGTTGGATCTCCATTGTTCTGCCGGAAAttt                 >  2:1404803/1‑140 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                                    >  1:1076378/1‑105 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                                    <  1:2423967/105‑1 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                                    <  2:1076378/105‑1 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                                    >  2:2423967/1‑105 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                 >  2:1733208/1‑140 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                 >  2:1689537/1‑140 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                 >  2:1255191/1‑140 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                 >  2:2095039/1‑140 (MQ=255)
                                                                                                                            aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                 >  2:1100898/1‑140 (MQ=255)
                                                                                                                             gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGTCTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt                <  2:522978/140‑1 (MQ=255)
                                                                                                                             gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt                <  2:180039/140‑1 (MQ=255)
                                                                                                                             gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt                <  1:1626697/140‑1 (MQ=255)
                                                                                                                             gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt                <  2:523437/140‑1 (MQ=255)
                                                                                                                               ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc              <  2:2079590/140‑1 (MQ=255)
                                                                                                                               ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc              <  2:614315/140‑1 (MQ=255)
                                                                                                                               ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc              <  2:2320622/140‑1 (MQ=255)
                                                                                                                               ggTTCGTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc              <  2:2320791/140‑1 (MQ=255)
                                                                                                                                 ttCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcccc            >  2:1129754/1‑140 (MQ=255)
                                                                                                                                 ttCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcccc            >  2:1923443/1‑140 (MQ=255)
                                                                                                                                         ccGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCa                                                  <  2:275947/94‑1 (MQ=255)
                                                                                                                                           gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt  >  2:497486/1‑140 (MQ=255)
                                                                                                                                           gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt  >  2:222845/1‑140 (MQ=255)
                                                                                                                                           gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt  >  2:2246625/1‑140 (MQ=255)
                                                                                                                                           gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt  >  2:700156/1‑140 (MQ=255)
                                                                                                                                           gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt  >  2:2412315/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GTTCCTTATCAATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATT  >  NZ_CP034943/2711803‑2712081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: