Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,711,951 C→T 8.0% Q120Q (CAG→CAA)  EO946_RS14015 ← ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,711,9510CT 8.0% 306.6 / 12.5 100Q120Q (CAG→CAA) EO946_RS14015ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base C (51/41);  new base T (2/6);  total (53/47)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

ATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATTCTCTAA  >  NZ_CP034943/2711814‑2712087
                                                                                                                                         |                                                                                                                                        
aTTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATGTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGGTCTTGTCCCGCCATCAGCCTg                                                                                                                                        >  2:134253/1‑140 (MQ=255)
  tCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGaa                                                                                                                                      >  1:2083803/1‑140 (MQ=255)
   ccGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAAt                                                                                                                                     <  1:491431/140‑1 (MQ=255)
    cGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATg                                                                                                                                    <  2:1175763/140‑1 (MQ=255)
    cGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATg                                                                                                                                    <  2:1739150/140‑1 (MQ=255)
      aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                                  >  1:131872/1‑140 (MQ=255)
      aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                                  >  1:1351187/1‑140 (MQ=255)
      aCAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGAc                                                                                                                                  >  1:1240133/1‑140 (MQ=255)
          agaTCGCAGCAAGAAAATCATTGATCGGATTTGTATTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATca                                                                                                                              <  2:2076801/140‑1 (MQ=255)
          agaTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATca                                                                                                                              <  2:457492/140‑1 (MQ=255)
            aTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATcaca                                                                                                                            >  1:985981/1‑140 (MQ=255)
            aTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATcaca                                                                                                                            >  1:905031/1‑140 (MQ=255)
            aTCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATcaca                                                                                                                            >  1:1531556/1‑140 (MQ=255)
              cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                          <  1:1256019/140‑1 (MQ=255)
              cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                          >  1:1229261/1‑140 (MQ=255)
              cGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                          <  1:1986173/140‑1 (MQ=255)
                           tCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGt                                                                                                             >  1:616837/1‑140 (MQ=255)
                             aGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCCTCAGCCTGAATGACATCACAGTCAATCCAAATCGTcc                                                                                                           >  1:1594319/1‑140 (MQ=255)
                               tGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATACAAATAGTCCGt                                                                                                         >  2:1859546/1‑140 (MQ=255)
                                       tttGTGTTGATCGTTCCCGCTTTGATCAGTTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCTTCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAg                                                                                                 <  1:465661/140‑1 (MQ=255)
                                            cTTGATCGTTCCCGCTTTGATCAGCGTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCtttt                                                                                            <  2:456795/140‑1 (MQ=255)
                                              tGATTGTTGCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCTAGAAATGCGCCTGTAATGGAATCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                                          <  2:1846151/140‑2 (MQ=255)
                                              tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                                          <  2:1846160/140‑2 (MQ=255)
                                              tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                                          <  2:1846058/140‑2 (MQ=255)
                                              tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTcc                                                                                          <  2:713573/140‑2 (MQ=255)
                                              tGATTGTTCCTGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTCCACCGAGCTTTTcc                                                                                          <  2:1048516/140‑2 (MQ=255)
                                              tGATTGTTCCTGCTATGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCGCCTGTAATGGAAGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGGTCACCGAGCTTTTcc                                                                                          <  2:594079/140‑2 (MQ=255)
                                              tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          <  2:919381/140‑1 (MQ=255)
                                              tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          <  1:1171471/140‑1 (MQ=255)
                                              tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          <  1:2212735/140‑1 (MQ=255)
                                              tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          <  2:1019725/140‑1 (MQ=255)
                                              tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          <  1:1304899/140‑1 (MQ=255)
                                              tGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          <  1:1369828/140‑1 (MQ=255)
                                              tGATCGGTCCCGCTTTGATCAGCGTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          <  1:1236237/140‑1 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                          <  2:1476010/106‑1 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGt                                                                                                                          >  1:1476010/1‑106 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                        >  1:32562/1‑140 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                        >  1:924741/1‑140 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                        >  1:648590/1‑140 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                        >  1:602384/1‑140 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                        >  1:544804/1‑140 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                        >  1:25282/1‑140 (MQ=255)
                                                aTCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTaa                                                                                        >  2:19041/1‑140 (MQ=255)
                                                    ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                                    >  2:378765/1‑140 (MQ=255)
                                                    ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                                    >  2:1981795/1‑140 (MQ=255)
                                                    ttCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCt                                                                                    >  2:1729200/1‑140 (MQ=255)
                                                     tCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTa                                                                                   >  1:385281/1‑140 (MQ=255)
                                                     tCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTACTAAATCtc                                                                                   >  1:675418/1‑139 (MQ=255)
                                                       ccGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCCCAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACg                                                                                 <  1:2138296/140‑1 (MQ=255)
                                                       ccGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACg                                                                                 <  2:82035/140‑1 (MQ=255)
                                                         gCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTGGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGAc                                                                               >  2:1343626/1‑140 (MQ=255)
                                                                    cTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          >  1:180362/1‑118 (MQ=255)
                                                                    cTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCt                                                                                          <  2:180362/118‑1 (MQ=255)
                                                                       gCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGGTCTTGTCCCGCCATCAGCCTGAATGACATCACTGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAgcgcgc                                                                 >  1:1148270/1‑140 (MQ=255)
                                                                               caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                                         <  2:218412/140‑1 (MQ=255)
                                                                               caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                                         <  2:621992/140‑1 (MQ=255)
                                                                               caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                                         <  2:623331/140‑1 (MQ=255)
                                                                               caatcgcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATc                                                         <  2:1472179/140‑1 (MQ=255)
                                                                                    gcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCg                                                    >  2:108002/1‑140 (MQ=255)
                                                                                    gcCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCg                                                    >  2:107791/1‑140 (MQ=255)
                                                                                        tAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGa                                                <  1:91348/140‑1 (MQ=255)
                                                                                        tAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGGTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGa                                                <  1:847122/140‑1 (MQ=255)
                                                                                             aGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAAtctc                                           <  2:32562/140‑1 (MQ=255)
                                                                                             aGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAAtctc                                           <  2:25282/140‑1 (MQ=255)
                                                                                                  tGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTg                                      >  1:1968957/1‑140 (MQ=255)
                                                                                                         gTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCACTCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGcc                               >  1:860321/1‑140 (MQ=255)
                                                                                                                 aGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCCGGATCTACAACAGCTCGAAGCGCGCGGCCGATCAGGCGTTGGATCTCCATTGTTCTGCCGGAAAttt                       >  2:1340154/1‑140 (MQ=255)
                                                                                                                 aGCTGTTCTAGTTCCTCCATCAGCTTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCCAGATCTACAACAGCTCGAAGCGCGCGGCCGATCAGGCGTTGGATCTCCATTGTTCTGCCGGAAAttt                       >  2:1404803/1‑140 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCCTCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                       >  2:944232/1‑140 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                                          >  1:1076378/1‑105 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                                          >  2:2423967/1‑105 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                                          <  1:2423967/105‑1 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGAt                                                          <  2:1076378/105‑1 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                       >  2:1255191/1‑140 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                       >  2:1689537/1‑140 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                       >  2:2095039/1‑140 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                       >  2:1733208/1‑140 (MQ=255)
                                                                                                                 aGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAttt                       >  2:1100898/1‑140 (MQ=255)
                                                                                                                  gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGTCTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt                      <  2:522978/140‑1 (MQ=255)
                                                                                                                  gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt                      <  2:523437/140‑1 (MQ=255)
                                                                                                                  gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt                      <  1:1626697/140‑1 (MQ=255)
                                                                                                                  gCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAAtttt                      <  2:180039/140‑1 (MQ=255)
                                                                                                                    ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc                    <  2:2320622/140‑1 (MQ=255)
                                                                                                                    ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc                    <  2:614315/140‑1 (MQ=255)
                                                                                                                    ggTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc                    <  2:2079590/140‑1 (MQ=255)
                                                                                                                    ggTTCGTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcc                    <  2:2320791/140‑1 (MQ=255)
                                                                                                                      ttCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcccc                  >  2:1129754/1‑140 (MQ=255)
                                                                                                                      ttCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTcccc                  >  2:1923443/1‑140 (MQ=255)
                                                                                                                              ccGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCa                                                        >  1:275947/1‑94 (MQ=255)
                                                                                                                              ccGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCa                                                        <  2:275947/94‑1 (MQ=255)
                                                                                                                                gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt        >  2:700156/1‑140 (MQ=255)
                                                                                                                                gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt        >  2:2246625/1‑140 (MQ=255)
                                                                                                                                gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt        >  2:497486/1‑140 (MQ=255)
                                                                                                                                gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt        >  2:2412315/1‑140 (MQ=255)
                                                                                                                                gCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGAtt        >  2:222845/1‑140 (MQ=255)
                                                                                                                                  cATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATtct      <  1:107791/140‑1 (MQ=255)
                                                                                                                                  cATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATtct      <  1:108002/140‑1 (MQ=255)
                                                                                                                                      aGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATTCTCTaa  >  2:798160/1‑140 (MQ=255)
                                                                                                                                      aGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATTCTCTaa  >  2:1510480/1‑140 (MQ=255)
                                                                                                                                      aGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATTCTCTaa  >  2:987686/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ATTCCGACAGAGATCGCAGCAAGAAAATCAGTGATCGGATTTGTCTTGATCGTTCCCGCTTTGATCAGCTTGCCAATCGCAATCGCCATAGCAAGAAATGCACCTGTAATGGAAGCGGTTCTTGTCCCGCCATCAGCCTGAATGACATCACAGTCAATCCAAATCGTCCGTTCACCGAGCTTTTCTAAATCTACGACTGCCCGAAGCGCGCGGCCGATCAGGCGTTGAATCTCCATTGTTCTGCCGGAAATTTTCCCCTTAGAAGATTCTCTAA  >  NZ_CP034943/2711814‑2712087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑