Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,720,740 G→A 5.2% C143C (TGC→TGT EO946_RS14065 ← aspartate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,720,7400GA 5.2% 566.3 / 12.9 173C143C (TGC→TGTEO946_RS14065aspartate kinase
Reads supporting (aligned to +/- strand):  ref base G (121/43);  new base A (6/3);  total (127/46)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01

Only 100 of 219 total aligned reads displayed.

TCGCTGATTTCACATAGCGCGGATCGGTAGTAAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGC  >  NZ_CP034943/2720601‑2720879
                                                                                                                                           |                                                                                                                                           
  gCTGATTTCACATAGCGCGGATCGGTAGTAAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCa                                                                                                                                           <  1:417479/140‑1 (MQ=255)
     gATTTCACATAGCGCGGATCGGTAGTAAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATc                                                                                                                                        >  2:1124018/1‑140 (MQ=255)
     gATTTCACATAGCGCGGATCGGTAGTAAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTAGCAATc                                                                                                                                        >  2:301430/1‑140 (MQ=255)
       tttCACATAGCGCGGATCGGTAGTAAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCct                                                                                                                                      <  1:1257665/140‑1 (MQ=255)
               aGCGCGGATCGGTAGTAAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATg                                                                                                                              <  1:1930697/140‑1 (MQ=255)
                gcgcGGCTCGGTAGTAAACACGCCGGGCACGTCCGTGGATATGTCACATGGATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTCTCTGCACCGCCCCGGGCCAAGGTGGTGATTTAGCACTGCTCTGTCAtcg                                                                                                                             >  2:1397985/1‑138 (MQ=255)
                      aTCGGTAGTAAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCACCTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGa                                                                                                                       >  2:1788057/1‑140 (MQ=255)
                           tagtaAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTGATGCCTTGGAATCCt                                                                                                                  >  1:2255012/1‑140 (MQ=255)
                           tagtaAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGACTCCt                                                                                                                  >  2:415307/1‑140 (MQ=255)
                           tagtaAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCt                                                                                                                  >  1:1795573/1‑140 (MQ=255)
                           tagtaAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCt                                                                                                                  >  2:533114/1‑140 (MQ=255)
                                aaCACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgac                                                                                                             >  2:972804/1‑140 (MQ=255)
                                aaCACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgac                                                                                                             >  2:1097394/1‑140 (MQ=255)
                                aaCACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgac                                                                                                             >  1:327059/1‑140 (MQ=255)
                                aaCACGCCGGGCACGTCCGGGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgac                                                                                                             >  1:713192/1‑140 (MQ=255)
                                     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                        >  1:825764/1‑140 (MQ=255)
                                     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                        >  1:2424233/1‑140 (MQ=255)
                                     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                        >  1:1770799/1‑140 (MQ=255)
                                     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                        >  1:1573805/1‑140 (MQ=255)
                                     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                        >  2:692952/1‑140 (MQ=255)
                                        gggCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatgacaa                                                                                                     >  1:1096558/1‑140 (MQ=255)
                                               tCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGAAAATTTTTcc                                                                                              >  1:524047/1‑140 (MQ=255)
                                                   tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                          >  2:364248/1‑140 (MQ=255)
                                                   tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                          >  2:911943/1‑140 (MQ=255)
                                                   tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                          <  1:2202340/140‑1 (MQ=255)
                                                   tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                          >  1:1106686/1‑140 (MQ=255)
                                                   tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAAGGACAATTTTTCCTTGc                                                                                          >  2:1468491/1‑140 (MQ=255)
                                                          gTCACCTTTATCCGCTTTTAATGCGGCAGCCCAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGAGTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                   >  2:1252592/1‑140 (MQ=255)
                                                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCACTCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                   >  2:2096226/1‑140 (MQ=255)
                                                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                   >  2:874550/1‑140 (MQ=255)
                                                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                   >  1:739148/1‑140 (MQ=255)
                                                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                   >  1:1359046/1‑140 (MQ=255)
                                                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                   >  2:514783/1‑140 (MQ=255)
                                                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                   >  2:1642667/1‑140 (MQ=255)
                                                            cacaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTgg                                                                                 >  1:1066292/1‑140 (MQ=255)
                                                             acaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGt                                                                                >  1:1840446/1‑140 (MQ=255)
                                                             acaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGt                                                                                >  2:88239/1‑140 (MQ=255)
                                                             acaTTTATCAGCTTTTAAAGCGGCAGCCAAAGCAACTGCTGTTGTATCTGAACCGCCCCGGCCCAATGTCGTAATTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGt                                                                                >  1:1553255/1‑140 (MQ=255)
                                                             acaTTTATCAGCTTTTAAAGCGGCAGCCAAAGCAACTGCTGTTGTATCTGAACCGCCCCGGCCCAATGTCGTAATTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGt                                                                                >  1:679008/1‑140 (MQ=255)
                                                               aTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCt                                                                              >  1:2226977/1‑140 (MQ=255)
                                                                   atactcTTTTAATGCGGCAGCAAAATAAAATGCAGTTGTATCTGAACCTACCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                          >  2:135542/6‑140 (MQ=255)
                                                                   atactcTTTTAATCCGGCAGAAAAATAAAATGCATTTGTATCTGAAAAGACCCGGCCCAATGTAGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                          >  2:2147186/6‑140 (MQ=255)
                                                                   aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                          >  1:930085/1‑140 (MQ=255)
                                                                   aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                          >  1:487463/1‑140 (MQ=255)
                                                                   aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAAGGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                          >  1:1524132/1‑140 (MQ=255)
                                                                     ccGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTaaa                                                                        >  2:1036013/1‑140 (MQ=255)
                                                                         ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                    >  1:2314046/1‑140 (MQ=255)
                                                                           ttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGaa                                                                  <  2:1068011/140‑1 (MQ=255)
                                                                                  ggCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGa                                                           <  1:476764/140‑1 (MQ=255)
                                                                                   gCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGat                                                          >  1:893741/1‑140 (MQ=255)
                                                                                   gCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGat                                                          <  1:2395063/140‑1 (MQ=255)
                                                                                        cAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCtg                                                     <  1:1615167/140‑1 (MQ=255)
                                                                                                 tGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTgg                                            >  2:2254016/1‑140 (MQ=255)
                                                                                                     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                        >  2:1962424/1‑140 (MQ=255)
                                                                                                     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                        >  2:620974/1‑140 (MQ=255)
                                                                                                           tCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTg                                  <  2:169923/140‑1 (MQ=255)
                                                                                                             tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                <  2:2101955/140‑1 (MQ=255)
                                                                                                             tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                <  2:1583118/140‑1 (MQ=255)
                                                                                                              gAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCct                                                                                                                                      >  1:2323106/1‑37 (MQ=255)
                                                                                                              gAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCct                                                                                                                                      <  2:2323106/37‑1 (MQ=255)
                                                                                                              gAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAAc                               <  2:487158/140‑1 (MQ=255)
                                                                                                              gAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAAc                               >  1:353497/1‑140 (MQ=255)
                                                                                                                aCCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAAtt                                                                                                   <  2:1538101/70‑1 (MQ=255)
                                                                                                                   gCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCtt                          >  1:201373/1‑140 (MQ=255)
                                                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACCATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCTAAGTCTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                         >  1:168871/1‑140 (MQ=255)
                                                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCACGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                         >  2:1190387/1‑140 (MQ=255)
                                                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGTACGGCTTc                         >  1:2400214/1‑140 (MQ=255)
                                                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                         >  2:1597092/1‑140 (MQ=255)
                                                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                         >  2:735940/1‑140 (MQ=255)
                                                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                         >  2:1814061/1‑140 (MQ=255)
                                                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                         >  1:385110/1‑140 (MQ=255)
                                                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                         >  1:1952868/1‑140 (MQ=255)
                                                                                                                         gCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTAc                    >  1:255450/1‑140 (MQ=255)
                                                                                                                               tGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTc              >  1:1172616/1‑140 (MQ=255)
                                                                                                                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc            >  1:1100506/3‑137 (MQ=255)
                                                                                                                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc            >  1:1732100/3‑137 (MQ=255)
                                                                                                                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc            <  2:1100529/135‑1 (MQ=255)
                                                                                                                                    tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc         >  2:252134/1‑140 (MQ=255)
                                                                                                                                    tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc         >  1:1869721/1‑140 (MQ=255)
                                                                                                                                    tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc         >  2:622398/1‑140 (MQ=255)
                                                                                                                                    tGATTTCGCAATCCTCTGTAATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc         >  2:643289/1‑140 (MQ=255)
                                                                                                                                      aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg       <  1:1387654/140‑1 (MQ=255)
                                                                                                                                         tCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTc                                                                                               >  1:803192/1‑49 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACATTTTTTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:1468491/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:1036013/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  2:930085/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:621220/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:735856/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:911943/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:1250408/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:200910/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:1344956/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  1:1938209/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  2:2431394/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  2:1840446/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  2:2226977/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  2:1905963/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  2:201373/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAACCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  2:2041335/140‑1 (MQ=255)
                                                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc  <  2:1512703/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TCGCTGATTTCACATAGCGCGGATCGGTAGTAAACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGC  >  NZ_CP034943/2720601‑2720879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: