BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,720,770	G→A	5.3%	V133V (GTC→GTT)	EO946_RS14065 ←	aspartate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,720,770	0	G	A	5.3%	742.0 / 19.1	225	V133V (GTC→GTT)	EO946_RS14065	aspartate kinase
Reads supporting (aligned to +/- strand): ref base G (102/111); new base A (6/6); total (108/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 248 total aligned reads displayed.

AACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTT  >  NZ_CP034943/2720633‑2720908

aaCACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATTCGGCAGCCAAAGCAACTGCAGTTGTATCTGAAACGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgac                                                                                                                                          >  2:621220/1‑140 (MQ=255)

aaCACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgac                                                                                                                                          >  2:972804/1‑140 (MQ=255)

aaCACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgac                                                                                                                                          >  2:1803934/1‑140 (MQ=255)

aaCACGCCGGGCACGTCCGGGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgac                                                                                                                                          >  1:713192/1‑140 (MQ=255)

     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                                                     >  1:1421242/1‑140 (MQ=255)

     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                                                     >  1:2214827/1‑140 (MQ=255)

     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                                                     >  1:825764/1‑140 (MQ=255)

     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                                                     >  1:1573805/1‑140 (MQ=255)

     gCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGGTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatga                                                                                                                                     >  1:177787/1‑140 (MQ=255)

         ggCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCgacaatgacaat                                                                                                                                 >  1:1905963/1‑140 (MQ=255)

               tCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGAAAATTTTTcc                                                                                                                           >  1:524047/1‑140 (MQ=255)

                   tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                       >  1:1106686/1‑140 (MQ=255)

                   tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                       >  2:911943/1‑140 (MQ=255)

                   tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAAGGACAATTTTTCCTTGc                                                                                                                       >  2:1468491/1‑140 (MQ=255)

                     tataTGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCACTGTCGTGATTTCGCAATCCTCTGTCATGACTTGGAATACTGCGACAATGACAATTTTTCCTTGCTc                                                                                                                     >  2:164451/1‑140 (MQ=255)

                     tataTGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTACTTGCTc                                                                                                                     >  2:1588835/1‑140 (MQ=255)

                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCACTCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                >  2:2096226/1‑140 (MQ=255)

                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                >  2:1250408/1‑140 (MQ=255)

                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                >  2:514783/1‑140 (MQ=255)

                          gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                >  2:874550/1‑140 (MQ=255)

                            cacaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTgg                                                                                                              >  1:1068011/1‑140 (MQ=255)

                             acaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGt                                                                                                             >  2:884359/1‑140 (MQ=255)

                               aTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCt                                                                                                           >  1:1167956/1‑140 (MQ=255)

                                   atactcTTTTAATCCGGCAGAAAAATAAAATGCATTTGTATCTGAAAAGACCCGGCCCAATGTAGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                       >  2:2147186/6‑140 (MQ=255)

                                   aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                       >  1:487463/1‑140 (MQ=255)

                                   aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAAGGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                       >  1:1524132/1‑140 (MQ=255)

                                     ccGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTaaa                                                                                                     >  2:1036013/1‑140 (MQ=255)

                                     ccGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTaaa                                                                                                     >  2:200910/1‑140 (MQ=255)

                                         ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                                                 >  1:645548/1‑140 (MQ=255)

                                         ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                                                 >  1:2406118/1‑140 (MQ=255)

                                             aaTGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAgt                                                                                             >  1:2013399/1‑140 (MQ=255)

                                                  ggCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGa                                                                                        <  2:370867/140‑1 (MQ=255)

                                                   gCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGat                                                                                       >  1:893741/1‑140 (MQ=255)

                                                                 tGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTgg                                                                         >  2:2254016/1‑140 (MQ=255)

                                                                     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                                                     >  2:338498/1‑140 (MQ=255)

                                                                     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                                                     >  2:622741/1‑140 (MQ=255)

                                                                     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                                                     >  2:1962424/1‑140 (MQ=255)

                                                                             tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                                             >  1:2316659/1‑140 (MQ=255)

                                                                              gAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAAc                                                            >  1:353497/1‑140 (MQ=255)

                                                                                aCCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAAtt                                                                                                                                <  2:1538101/70‑1 (MQ=255)

                                                                                   gCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCtt                                                       <  2:2348423/140‑1 (MQ=255)

                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACCATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCTAAGTCTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                      >  1:168871/1‑140 (MQ=255)

                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCACGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                      >  2:1190387/1‑140 (MQ=255)

                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                      >  1:2400120/1‑140 (MQ=255)

                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                      >  2:2038318/1‑140 (MQ=255)

                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                      >  1:385119/1‑140 (MQ=255)

                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                      >  2:735856/1‑140 (MQ=255)

                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                      >  1:1952868/1‑140 (MQ=255)

                                                                                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                      >  1:915434/1‑140 (MQ=255)

                                                                                         gCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTAc                                                 >  1:255450/1‑140 (MQ=255)

                                                                                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                         <  2:1732100/135‑1 (MQ=255)

                                                                                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                         >  1:1732100/3‑137 (MQ=255)

                                                                                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                         <  2:1100506/135‑1 (MQ=255)

                                                                                                    tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                      >  1:1869721/1‑140 (MQ=255)

                                                                                                      aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                    <  2:1096558/140‑1 (MQ=255)

                                                                                                      aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                    <  1:1387654/140‑1 (MQ=255)

                                                                                                      aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                    <  2:161398/140‑1 (MQ=255)

                                                                                                         tCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTc                                                                                                                            <  2:803192/49‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  2:2226977/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:2251963/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:1344956/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:1097394/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:338498/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  2:1905963/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  2:1840446/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:135542/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:543699/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:998770/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:2147186/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:735856/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  2:1521409/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:741649/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:1036013/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:1250408/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  1:1938209/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  2:1150927/140‑1 (MQ=255)

                                                                                                           gCAATCCTCTGTCAGGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  2:1387133/140‑1 (MQ=255)

                                                                                                           gCAATACTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  2:964260/140‑1 (MQ=255)

                                                                                                           acAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTCCCGCCTgccagc                               <  2:679008/139‑1 (MQ=255)

                                                                                                             aaTCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGCACGGCTTCCGTACGGATTCCCGCCTgccagcca                             <  2:1174797/140‑1 (MQ=255)

                                                                                                                ccTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGta                          <  1:1487620/140‑1 (MQ=255)

                                                                                                                ccTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGta                          <  1:966157/140‑1 (MQ=255)

                                                                                                                  tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                        <  2:546208/140‑1 (MQ=255)

                                                                                                                    tctCAAGCCTTTTAATCCTGCGAAAATCACAATTTTTCCTTGCTAAAGCTCGTCTGCTAAAAACGAAGTGTCGATATCTGTGAGACTGGCGTTTCCCTGAACGGAATCAGTACGGATTCCCTCCTGCCAGCCATTCTAgc                      >  2:1315824/3‑139 (MQ=255)

                                                                                                                    tGTCATGCCGTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAgg                      >  1:1189279/1‑140 (MQ=255)

                                                                                                                       cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                   <  1:1803934/140‑1 (MQ=255)

                                                                                                                       cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                   <  2:327059/140‑1 (MQ=255)

                                                                                                                         ttgcTTGGAATCCTCCGACTATTAAAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCTATGTGTCGATATCTTTGATCCTGGCGTTTCCGTGAACGGCTTACGTACGGATTCCCGCGTGCGAGCCACTATTTGAcaca                 <  2:485120/137‑1 (MQ=255)

                                                                                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGTGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                 <  2:2427346/140‑1 (MQ=255)

                                                                                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                 <  1:970736/140‑1 (MQ=255)

                                                                                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                 >  1:161585/1‑140 (MQ=255)

                                                                                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                 <  1:317035/140‑1 (MQ=255)

                                                                                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                 >  1:2353328/1‑140 (MQ=255)

                                                                                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                 <  1:2074692/140‑1 (MQ=255)

                                                                                                                         ggCCTTGGAATCCTGCGACAATGACAATTTGTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                 <  2:1573805/139‑1 (MQ=255)

                                                                                                                              tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTc            <  1:415307/140‑1 (MQ=255)

                                                                                                                                     cTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt     >  1:15660/1‑140 (MQ=255)

                                                                                                                                     cTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt     >  1:1609847/1‑140 (MQ=255)

                                                                                                                                     cTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt     >  1:742366/1‑140 (MQ=255)

                                                                                                                                      tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                               <  2:1352078/113‑1 (MQ=255)

AACACGCCGGGCACGTCCGTGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTT  >  NZ_CP034943/2720633‑2720908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`