Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 438,591 C→A 5.8% K48N (AAG→AAT gabR ← transcriptional regulator GabR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943438,5910CA 5.8% 556.3 / 16.7 181K48N (AAG→AATgabRtranscriptional regulator GabR
Reads supporting (aligned to +/- strand):  ref base C (64/104);  new base A (4/7);  total (68/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 183 total aligned reads displayed.

GTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATG  >  NZ_CP034943/438452‑438729
                                                                                                                                           |                                                                                                                                          
gTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGc                                                                                                                                            >  1:37594/1‑140 (MQ=255)
gTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGc                                                                                                                                            >  1:117583/1‑140 (MQ=255)
 tGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCt                                                                                                                                           >  1:2075391/1‑140 (MQ=255)
      aTGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCg                                                                                                                                      >  2:2345580/1‑140 (MQ=255)
        ggggggTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGc                                                                                                                                    <  1:1053179/140‑1 (MQ=255)
         gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                   >  1:100111/3‑140 (MQ=255)
         gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                   >  1:397235/3‑140 (MQ=255)
           gggTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAg                                                                                                                                 >  2:524815/1‑140 (MQ=255)
             gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                               >  2:884682/1‑140 (MQ=255)
             gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                               <  2:925159/140‑1 (MQ=255)
             gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                               <  1:1402104/140‑1 (MQ=255)
             gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                               <  1:591277/140‑1 (MQ=255)
             gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                               <  1:1105800/140‑1 (MQ=255)
             gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                               <  2:961335/140‑1 (MQ=255)
             gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                               <  1:1961474/140‑1 (MQ=255)
             gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCATCAGCTTCTCGGCCAGct                                                                                                                               <  1:2415839/140‑1 (MQ=255)
             gTGCTCCACGGCAGAAAACACGTCCAGCGTCTCCACGAAAAACCACTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                               <  1:804716/140‑1 (MQ=255)
               gCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctct                                                                                                                             <  1:2015774/140‑1 (MQ=255)
                cTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctc                                                                                                                            >  2:266132/1‑140 (MQ=255)
                cTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctc                                                                                                                            >  1:1008180/1‑140 (MQ=255)
                  ccccGGCAGAAAACATGTCCAGCTTCTCCACCAAACACCCCTTTCGCTCAATGGCGTACAAATAGCCCTCAGCCAGAAGCTGCTGATAGGCTGAATCCACTGAGTTTACGCGGCCCTTCAGCTTCTCGGCCAGctctctc                                                                                                                          <  2:2121566/137‑1 (MQ=255)
                  ccACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctctc                                                                                                                          <  2:298003/140‑1 (MQ=255)
                   ctcggcggAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGCCAGctcccgct                                                                                                                         <  2:1205147/138‑6 (MQ=255)
                     cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                       >  1:1808203/1‑140 (MQ=255)
                     cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                       <  2:891957/140‑1 (MQ=255)
                       gCAGAACAAATGTCCAGCTTCTCCCCGAAAACCCCCTGTCGCTCAATGGCGTACAAATATCCCTCACCCAGAAGCTGCCGCTAGGCTGAATTCACTGAGTTTACGCTGACCTGCAGCTTCTCGGCCAGCTCTCTCTTGGa                                                                                                                     <  2:501739/140‑1 (MQ=255)
                        cAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAg                                                                                                                    >  2:411574/1‑140 (MQ=255)
                         aGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAgg                                                                                                                   <  1:1392547/140‑1 (MQ=255)
                               cATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCt                                                                                                             <  2:1481382/140‑1 (MQ=255)
                                  gTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCg                                                                                                          <  2:44127/140‑1 (MQ=255)
                                     cAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAgt                                                                                                       >  1:2245631/1‑140 (MQ=255)
                                            tcCACGTAAAACCCATTTCGCTCAATTGCGTTCAAATATCCCTCAGCCAGATGCTGCTGTTAGGCTGAATTCACTGAGTTGACGCTGACCTTCAGCTTCTCGGGCAGCTCTCTCTTGGAGGGGAGCTTCGAGTGTTgcag                                                                                                <  2:1348496/140‑1 (MQ=255)
                                            tcCACGAAAAACCCCTTTCGCTGAAGGGCGGACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGCTTACGCTGACCTTCAGGGTCTCGGCCAGCGCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                <  2:2014946/140‑1 (MQ=255)
                                            tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                <  2:1912651/140‑1 (MQ=255)
                                            tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                <  2:1367315/140‑1 (MQ=255)
                                            tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                <  1:2118609/140‑1 (MQ=255)
                                            tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                <  2:1336329/140‑1 (MQ=255)
                                            tcCACGAAAAACCCCTTTAGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                >  2:827976/1‑140 (MQ=255)
                                            gccACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCGGCTGATAGGCTGAAGTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                <  2:1607764/139‑1 (MQ=255)
                                                 gAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCGTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGAt                                                                                           <  1:1650396/140‑1 (MQ=255)
                                                        cccTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCt                                                                                    >  1:2277529/1‑140 (MQ=255)
                                                        cccTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCt                                                                                    >  1:2277541/1‑140 (MQ=255)
                                                             tCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGaa                                                                               >  1:2081827/1‑140 (MQ=255)
                                                             tCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGaa                                                                               >  1:1800483/1‑140 (MQ=255)
                                                                cTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAAttt                                                                            >  1:1811270/1‑140 (MQ=255)
                                                                     tGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCtttt                                                                       >  1:589286/1‑140 (MQ=255)
                                                                        cGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAgg                                                                                                                   >  1:1466645/1‑93 (MQ=255)
                                                                        cGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAgg                                                                                                                   <  2:1466645/93‑1 (MQ=255)
                                                                          tACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAg                                                                  >  1:2036642/1‑140 (MQ=255)
                                                                                  tCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGAt                                                          >  2:951733/1‑140 (MQ=255)
                                                                                  tCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGAt                                                          >  2:1369303/1‑140 (MQ=255)
                                                                                       cAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAAtt                                                     >  2:518340/1‑140 (MQ=255)
                                                                                             gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                               <  2:1683597/140‑1 (MQ=255)
                                                                                             gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                               <  1:1385850/140‑1 (MQ=255)
                                                                                             gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                               <  2:1503665/140‑1 (MQ=255)
                                                                                             gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                               <  2:2203670/140‑1 (MQ=255)
                                                                                             gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                               <  1:313707/140‑1 (MQ=255)
                                                                                             gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                               <  2:1795722/140‑1 (MQ=255)
                                                                                                    ctgATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGCATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGatat                                        >  1:721171/1‑140 (MQ=255)
                                                                                                    ctgATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTTCTGATAGatat                                        >  1:1271684/1‑140 (MQ=255)
                                                                                                      gacaGGCTGAATTCCCGGAGTTGACGCTGACCTTCAGCTTCTCGGCCAGCTCTCCCTTGGAGGGGACCGTCGAGGGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  2:1445558/137‑1 (MQ=255)
                                                                                                      gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  2:184262/140‑1 (MQ=255)
                                                                                                      gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  2:2277541/140‑1 (MQ=255)
                                                                                                      gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  2:1010415/140‑1 (MQ=255)
                                                                                                      gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  1:888229/140‑1 (MQ=255)
                                                                                                      gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  1:636970/140‑1 (MQ=255)
                                                                                                      gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  2:941325/140‑1 (MQ=255)
                                                                                                      gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTATCGGCCAGCTCTCTCTTGTATGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  2:2029402/140‑1 (MQ=255)
                                                                                                      gATAGGCTGAATTCACTGAGTTTACGCTGAACTTCATCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTTTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                      <  1:926920/140‑1 (MQ=255)
                                                                                                           gCTGACTTCACTGAGTTTACGCGGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  2:1174001/140‑1 (MQ=255)
                                                                                                           gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  2:2245631/140‑1 (MQ=255)
                                                                                                           gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  1:1070964/140‑1 (MQ=255)
                                                                                                           gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  2:1717626/140‑1 (MQ=255)
                                                                                                           gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  1:2311140/140‑1 (MQ=255)
                                                                                                           gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  1:1593134/140‑1 (MQ=255)
                                                                                                           gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  1:1547614/140‑1 (MQ=255)
                                                                                                           gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  1:430594/140‑1 (MQ=255)
                                                                                                           gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  1:518340/140‑1 (MQ=255)
                                                                                                           gCTGAATTCAATGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                 <  2:1374339/140‑1 (MQ=255)
                                                                                                            cTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCCGCTCTCTCTTGGAGGGGACCTTCGCCTGTGGCAGCAGATTACGGCTGAGACTTTCTTTTTTTAGTTTTTGATAAATTTGCTGATCGATATAATCGGCt                                >  2:1066710/1‑140 (MQ=255)
                                                                                                                     gtGAGTTTACTCTTAGCTTGAGGTTATCGTCCTGTTTTGTTTTGGTGGGGAGCTTCGATTGTGGCAGAATATTACTGCTGTTAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGTTAGTTATATTCGGTTTGTTTTGAg                       <  2:2174474/139‑1 (MQ=255)
                                                                                                                     cTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAg                       <  2:1392414/140‑1 (MQ=255)
                                                                                                                          tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                  >  1:1420899/1‑140 (MQ=255)
                                                                                                                          tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                  >  1:1149608/1‑140 (MQ=255)
                                                                                                                          tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                  <  1:448435/140‑1 (MQ=255)
                                                                                                                          tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                  <  1:1346434/140‑1 (MQ=255)
                                                                                                                          tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGGGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATAGTCTTGTTCTGAGCGGTc                  >  1:1234704/1‑140 (MQ=255)
                                                                                                                             tcGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg               <  2:1803053/139‑1 (MQ=255)
                                                                                                                             ccGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTGGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg               <  2:2133966/139‑1 (MQ=255)
                                                                                                                             aCGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg               <  2:1292592/140‑1 (MQ=255)
                                                                                                                              cGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAgt              <  2:633324/140‑1 (MQ=255)
                                                                                                                                 tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta           <  2:397235/140‑2 (MQ=255)
                                                                                                                                 tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta           <  2:100111/140‑2 (MQ=255)
                                                                                                                                 tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta           <  2:1127422/140‑2 (MQ=255)
                                                                                                                                 tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta           <  2:621177/140‑2 (MQ=255)
                                                                                                                                 tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta           <  2:1582267/140‑2 (MQ=255)
                                                                                                                                  gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa          <  1:889830/140‑1 (MQ=255)
                                                                                                                                  gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa          <  1:259773/140‑1 (MQ=255)
                                                                                                                                  gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa          >  1:2326792/1‑140 (MQ=255)
                                                                                                                                  gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa          >  1:1690238/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GTGCAAATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATG  >  NZ_CP034943/438452‑438729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: