BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,720,790	G→T	5.1%	Q127K (CAA→AAA)	EO946_RS14065 ←	aspartate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,720,790	0	G	T	5.1%	695.7 / 17.9	223	Q127K (CAA→AAA)	EO946_RS14065	aspartate kinase
Reads supporting (aligned to +/- strand): ref base G (101/110); new base T (6/6); total (107/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 223 total aligned reads displayed.

TGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAG  >  NZ_CP034943/2720652‑2720928

tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                                           >  2:741649/1‑140 (MQ=255)

tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                                           >  1:1106686/1‑140 (MQ=255)

tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                                           >  2:364248/1‑140 (MQ=255)

tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                                           >  2:911943/1‑140 (MQ=255)

  tataTGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCACTGTCGTGATTTCGCAATCCTCTGTCATGACTTGGAATACTGCGACAATGACAATTTTTCCTTGCTc                                                                                                                                         >  2:164451/1‑140 (MQ=255)

       gTCACCTTTATCCGCTTTTAATGCGGCAGCCCAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGAGTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                                    >  2:1252592/1‑140 (MQ=255)

       gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTTTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                                    >  1:1638153/1‑140 (MQ=255)

       gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                                    >  2:874550/1‑140 (MQ=255)

         cacaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTgg                                                                                                                                  >  1:1068011/1‑140 (MQ=255)

          acaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGt                                                                                                                                 >  2:884359/1‑140 (MQ=255)

          acaTTTATCAGCTTTTAAAGCGGCAGCCAAAGCAACTGCTGTTGTATCTGAACCGCCCCGGCCCAATGTCGTAATTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGt                                                                                                                                 >  1:1553255/1‑140 (MQ=255)

          acaTTTATCAGCTTTTAAAGCGGCAGCCAAAGCAACTGCTGTTGTATCTGAACCGCCCCGGCCCAATGTCGTAATTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGt                                                                                                                                 >  1:679008/1‑140 (MQ=255)

            aTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCt                                                                                                                               >  1:1167956/1‑140 (MQ=255)

                aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                                           >  1:1521409/1‑140 (MQ=255)

                aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                                           >  1:525481/1‑140 (MQ=255)

                aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                                           >  1:487463/1‑140 (MQ=255)

                 tCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTaa                                                                                                                          <  1:1854856/140‑1 (MQ=255)

                  ccGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTaaa                                                                                                                         >  2:200910/1‑140 (MQ=255)

                      ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                                                                     >  1:74222/1‑140 (MQ=255)

                      ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                                                                     >  1:73164/1‑140 (MQ=255)

                      ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                                                                     >  1:2314046/1‑140 (MQ=255)

                        ttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGaa                                                                                                                   <  2:1106686/140‑1 (MQ=255)

                        ttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGaa                                                                                                                   <  2:1066292/140‑1 (MQ=255)

                        ttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGaa                                                                                                                   <  2:2271783/140‑1 (MQ=255)

                               ggCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGa                                                                                                            <  1:476764/140‑1 (MQ=255)

                                gCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGat                                                                                                           <  1:1401695/140‑1 (MQ=255)

                                gCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGat                                                                                                           >  1:893741/1‑140 (MQ=255)

                                     cAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCtg                                                                                                      <  1:1615167/140‑1 (MQ=255)

                                     cAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGGGTCGATATCtg                                                                                                      <  1:192573/140‑1 (MQ=255)

                                             cTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTg                                                                                              <  1:335774/140‑1 (MQ=255)

                                             cTGCAGGTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTg                                                                                              <  1:2082937/140‑1 (MQ=255)

                                                  gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                                                                         >  2:1962424/1‑140 (MQ=255)

                                                          tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                                                                 <  2:2101955/140‑1 (MQ=255)

                                                          tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                                                                 <  2:1583118/140‑1 (MQ=255)

                                                                gCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCtt                                                                           >  1:201373/1‑140 (MQ=255)

                                                                gCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCtt                                                                           <  2:2348423/140‑1 (MQ=255)

                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACCATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCTAAGTCTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                          >  1:168871/1‑140 (MQ=255)

                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGTACGGCTTc                                                                          >  1:2400214/1‑140 (MQ=255)

                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                          >  1:385110/1‑140 (MQ=255)

                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                          >  2:2038318/1‑140 (MQ=255)

                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                          >  2:735856/1‑140 (MQ=255)

                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                          >  2:1597092/1‑140 (MQ=255)

                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                          >  1:1952868/1‑140 (MQ=255)

                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                          >  2:1814061/1‑140 (MQ=255)

                                                                      gCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTAc                                                                     >  1:255450/1‑140 (MQ=255)

                                                                           aTGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGAtt                                                                >  1:1371286/1‑140 (MQ=255)

                                                                                 taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                             >  1:1100506/3‑137 (MQ=255)

                                                                                 taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                             <  2:1732100/135‑1 (MQ=255)

                                                                                 taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                             <  2:1100506/135‑1 (MQ=255)

                                                                                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                          >  2:643938/1‑140 (MQ=255)

                                                                                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                          >  2:2074692/1‑140 (MQ=255)

                                                                                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                          >  2:622398/1‑140 (MQ=255)

                                                                                   aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                                        <  2:161398/140‑1 (MQ=255)

                                                                                     ttCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgcc                                                      <  2:1508202/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACATTTTTTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:1468491/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:1036013/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:1521409/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:1840446/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:1905963/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:1250408/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:2226977/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:1288901/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:1167956/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:487463/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:525481/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:911943/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:809655/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:1597092/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:735940/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:621220/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:556716/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:200910/140‑1 (MQ=255)

                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  1:2251963/140‑1 (MQ=255)

                                                                                        gCAATACTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   <  2:964260/140‑1 (MQ=255)

                                                                                        acAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTCCCGCCTgccagc                                                   <  2:1553255/139‑1 (MQ=255)

                                                                                          aaTCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagcca                                                 <  2:1359046/140‑1 (MQ=255)

                                                                                             ccTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGta                                              <  2:1191738/140‑1 (MQ=255)

                                                                                             ccTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGta                                              <  1:966157/140‑1 (MQ=255)

                                                                                               tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                            <  2:1033799/140‑1 (MQ=255)

                                                                                               tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                            <  2:335613/140‑1 (MQ=255)

                                                                                                 tctCAAGCCTTTTAATCCTGCGAAAATCACAATTTTTCCTTGCTAAAGCTCGTCTGCTAAAAACGAAGTGTCGATATCTGTGAGACTGGCGTTTCCCTGAACGGAATCAGTACGGATTCCCTCCTGCCAGCCATTCTAgc                                          >  2:1315824/3‑139 (MQ=255)

                                                                                                 tGTCATGCCGTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAgg                                          >  1:1189279/1‑140 (MQ=255)

                                                                                                    cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                       <  1:1803934/140‑1 (MQ=255)

                                                                                                    cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                       <  1:217889/140‑1 (MQ=255)

                                                                                                      ttgcTTGGAATCCTCCGACTATTAAAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCTATGTGTCGATATCTTTGATCCTGGCGTTTCCGTGAACGGCTTACGTACGGATTCCCGCGTGCGAGCCACTATTTGAcaca                                     <  2:485120/137‑1 (MQ=255)

                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGTGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                     <  2:2427346/140‑1 (MQ=255)

                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                     >  1:98488/1‑140 (MQ=255)

                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                     <  1:970736/140‑1 (MQ=255)

                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                     <  1:2364405/140‑1 (MQ=255)

                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                     >  1:1889990/1‑140 (MQ=255)

                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                     <  2:1421242/140‑1 (MQ=255)

                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                     <  2:1181353/140‑1 (MQ=255)

                                                                                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTa                                                                      <  2:352696/102‑1 (MQ=255)

                                                                                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTa                                                                      >  1:352696/1‑102 (MQ=255)

                                                                                                                   tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   >  1:2333874/1‑113 (MQ=255)

                                                                                                                   tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                   >  1:1352078/1‑113 (MQ=255)

                                                                                                                   tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCtt                        >  1:39401/1‑140 (MQ=255)

                                                                                                                          atgacaatTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTg                 >  2:261065/1‑140 (MQ=255)

                                                                                                                          atgacaatTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTg                 >  2:1347378/1‑140 (MQ=255)

                                                                                                                          atgacaatTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTg                 >  2:927589/1‑140 (MQ=255)

TGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAG  >  NZ_CP034943/2720652‑2720928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`