Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,720,791 C→T 5.5% E126E (GAG→GAA EO946_RS14065 ← aspartate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,720,7910CT 5.5% 712.0 / 19.2 216E126E (GAG→GAAEO946_RS14065aspartate kinase
Reads supporting (aligned to +/- strand):  ref base C (95/109);  new base T (6/6);  total (101/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 224 total aligned reads displayed.

TGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCA  >  NZ_CP034943/2720652‑2720930
                                                                                                                                           |                                                                                                                                           
tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                                             >  2:364248/1‑140 (MQ=255)
tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                                             >  1:1106686/1‑140 (MQ=255)
tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                                             >  2:741649/1‑140 (MQ=255)
tgtATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGc                                                                                                                                             >  2:911943/1‑140 (MQ=255)
  tataTGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCACTGTCGTGATTTCGCAATCCTCTGTCATGACTTGGAATACTGCGACAATGACAATTTTTCCTTGCTc                                                                                                                                           >  2:164451/1‑140 (MQ=255)
       gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTTTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                                      >  1:1638153/1‑140 (MQ=255)
       gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                                      >  2:874550/1‑140 (MQ=255)
       gTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCt                                                                                                                                      >  2:1250408/1‑140 (MQ=255)
         cacaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTgg                                                                                                                                    >  1:1068011/1‑140 (MQ=255)
         cacaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTgg                                                                                                                                    >  1:2271783/1‑140 (MQ=255)
          acaTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGt                                                                                                                                   >  2:884359/1‑140 (MQ=255)
            aTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCt                                                                                                                                 >  1:1167956/1‑140 (MQ=255)
            aTTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCt                                                                                                                                 >  1:2226977/1‑140 (MQ=255)
                atactcTTTTAATGCGGCAGCAAAATAAAATGCAGTTGTATCTGAACCTACCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                                             >  2:135542/6‑140 (MQ=255)
                aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                                             >  1:525481/1‑140 (MQ=255)
                aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                                             >  1:1521409/1‑140 (MQ=255)
                aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                                             >  1:487463/1‑140 (MQ=255)
                aTCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTa                                                                                                                             >  1:930085/1‑140 (MQ=255)
                 tCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTaa                                                                                                                            <  1:1854856/140‑1 (MQ=255)
                  ccGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTaaa                                                                                                                           >  2:200910/1‑140 (MQ=255)
                      ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                                                                       >  1:645548/1‑140 (MQ=255)
                      ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                                                                       >  1:73164/1‑140 (MQ=255)
                      ttttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCg                                                                                                                       >  1:2406118/1‑140 (MQ=255)
                        ttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGaa                                                                                                                     <  2:2271783/140‑1 (MQ=255)
                        ttAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGaa                                                                                                                     <  2:1066292/140‑1 (MQ=255)
                          aaTGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAgt                                                                                                                   >  1:2013399/1‑140 (MQ=255)
                                gCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGat                                                                                                             >  1:893741/1‑140 (MQ=255)
                                gCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGat                                                                                                             <  1:1401695/140‑1 (MQ=255)
                                     cAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCtg                                                                                                        <  1:1615167/140‑1 (MQ=255)
                                     cAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGGGTCGATATCtg                                                                                                        <  1:192573/140‑1 (MQ=255)
                                             cTGCAGGTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTg                                                                                                <  1:2082937/140‑1 (MQ=255)
                                                          tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                                                                   >  1:2316659/1‑140 (MQ=255)
                                                          tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                                                                   <  2:2101955/140‑1 (MQ=255)
                                                                gCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCtt                                                                             <  2:2348423/140‑1 (MQ=255)
                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCACGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                            >  2:1190387/1‑140 (MQ=255)
                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                            >  2:1814061/1‑140 (MQ=255)
                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                            >  2:1597092/1‑140 (MQ=255)
                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                            >  2:2038318/1‑140 (MQ=255)
                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                            >  1:385119/1‑140 (MQ=255)
                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                            >  2:735856/1‑140 (MQ=255)
                                                                 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                            >  1:2400120/1‑140 (MQ=255)
                                                                           aTGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGAtt                                                                  >  1:1371286/1‑140 (MQ=255)
                                                                                 taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                               >  1:1100506/3‑137 (MQ=255)
                                                                                 taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                               >  1:1732100/3‑137 (MQ=255)
                                                                                 taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                               <  2:1100529/135‑1 (MQ=255)
                                                                                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                            >  2:2074692/1‑140 (MQ=255)
                                                                                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                            >  2:252134/1‑140 (MQ=255)
                                                                                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                            >  2:622398/1‑140 (MQ=255)
                                                                                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                            >  2:643938/1‑140 (MQ=255)
                                                                                   aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTGTGCTAAAACCGAAGTGTCTATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                                          <  2:2150867/140‑1 (MQ=255)
                                                                                   aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                                          <  2:161398/140‑1 (MQ=255)
                                                                                   aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                                          <  2:1096558/140‑1 (MQ=255)
                                                                                     ttCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgcc                                                        <  2:1508202/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACATTTTTTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:1468491/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:487463/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:1288901/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:1840446/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:1521409/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:1250408/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:1905963/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:1036013/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:1597092/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:1167956/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:1962424/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:911943/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:741649/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:735940/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:556716/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:525481/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:543699/140‑1 (MQ=255)
                                                                                        gCAATCCTCTGTCAGGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:1387133/140‑1 (MQ=255)
                                                                                        gCAATACTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  2:964260/140‑1 (MQ=255)
                                                                                        gCAAGCCTCTGTCCTGCCTTCGAATCCTGCGACACTGACAATTTGTCCGGGCTCAAGCGGGTCTGCTACAACCGCACGGTCGATATCAGTGATCCGGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     <  1:364248/140‑1 (MQ=255)
                                                                                        acAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTCCCGCCTgccagc                                                     <  2:1553255/139‑1 (MQ=255)
                                                                                               tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                              <  2:1704917/140‑1 (MQ=255)
                                                                                               tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                              <  2:1033799/140‑1 (MQ=255)
                                                                                               tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                              <  2:335613/140‑1 (MQ=255)
                                                                                                 tctCAAGCCTTTTAATCCTGCGAAAATCACAATTTTTCCTTGCTAAAGCTCGTCTGCTAAAAACGAAGTGTCGATATCTGTGAGACTGGCGTTTCCCTGAACGGAATCAGTACGGATTCCCTCCTGCCAGCCATTCTAgc                                            >  2:1315824/3‑139 (MQ=255)
                                                                                                 tGTCATGCCGTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAgg                                            >  1:1189279/1‑140 (MQ=255)
                                                                                                    cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                         <  1:1803934/140‑1 (MQ=255)
                                                                                                    cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                         <  1:217889/140‑1 (MQ=255)
                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGTGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                       <  2:2427346/140‑1 (MQ=255)
                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCCGTATAGGAcaca                                       >  2:1955071/1‑140 (MQ=255)
                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                       <  1:317035/140‑1 (MQ=255)
                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                       <  2:42923/140‑1 (MQ=255)
                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                       <  1:2364405/140‑1 (MQ=255)
                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                       <  1:970725/140‑1 (MQ=255)
                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                       >  1:98488/1‑140 (MQ=255)
                                                                                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                       <  2:1181353/140‑1 (MQ=255)
                                                                                                      ggCCTTGGAATCCTGCGACAATGACAATTTGTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                       <  2:1573805/139‑1 (MQ=255)
                                                                                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTa                                                                        >  1:352696/1‑102 (MQ=255)
                                                                                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTa                                                                        <  2:352696/102‑1 (MQ=255)
                                                                                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTc                                  <  1:415307/140‑1 (MQ=255)
                                                                                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTc                                  >  1:1945652/1‑140 (MQ=255)
                                                                                                                  cTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt                           >  1:742366/1‑140 (MQ=255)
                                                                                                                  cTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt                           >  1:15660/1‑140 (MQ=255)
                                                                                                                   tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                     >  1:1352078/1‑113 (MQ=255)
                                                                                                                          atgacaatTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTg                   >  2:927589/1‑140 (MQ=255)
                                                                                                                          atgacaatTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTg                   >  2:1347378/1‑140 (MQ=255)
                                                                                                                                           cTCAAGCTGGTCTGCTAAAACCGACGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCa  <  1:1872481/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TGTATATGTCACATTTATCCGCTTTTAATGCGGCAGCCAAAGCAACTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCA  >  NZ_CP034943/2720652‑2720930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: