BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,720,830	G→A	5.2%	I113I (ATC→ATT)	EO946_RS14065 ←	aspartate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,720,830	0	G	A	5.2%	578.2 / 13.0	173	I113I (ATC→ATT)	EO946_RS14065	aspartate kinase
Reads supporting (aligned to +/- strand): ref base G (58/106); new base A (3/6); total (61/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 176 total aligned reads displayed.

CTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATA  >  NZ_CP034943/2720697‑2720968

cTGCAGGTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTg                                                                                                                                      <  1:2082937/140‑1 (MQ=255)

 tGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTgg                                                                                                                                     >  2:2254016/1‑140 (MQ=255)

     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                                                                                                                 >  2:338498/1‑140 (MQ=255)

     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                                                                                                                 >  2:620974/1‑140 (MQ=255)

     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                                                                                                                 >  2:1962424/1‑140 (MQ=255)

     gTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGtt                                                                                                                                 >  2:622741/1‑140 (MQ=255)

           tCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTg                                                                                                                           <  2:169923/140‑1 (MQ=255)

             tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                                                                                                         <  2:2101955/140‑1 (MQ=255)

             tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                                                                                                         >  1:2316659/1‑140 (MQ=255)

             tGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGaa                                                                                                                         <  2:1583118/140‑1 (MQ=255)

              gAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAAc                                                                                                                        <  2:487158/140‑1 (MQ=255)

              gAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAAc                                                                                                                        >  1:353497/1‑140 (MQ=255)

                   gCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCtt                                                                                                                   >  1:201373/1‑140 (MQ=255)

                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACCATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCTAAGTCTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                  >  1:168871/1‑140 (MQ=255)

                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCACGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                  >  2:1190387/1‑140 (MQ=255)

                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                  >  1:2400120/1‑140 (MQ=255)

                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                  >  1:385119/1‑140 (MQ=255)

                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                  >  1:1952868/1‑140 (MQ=255)

                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                  >  1:915434/1‑140 (MQ=255)

                    ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                  >  2:735856/1‑140 (MQ=255)

                         gCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTAc                                                                                                             >  1:255450/1‑140 (MQ=255)

                              aTGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGAtt                                                                                                        >  1:1371286/1‑140 (MQ=255)

                               tGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTc                                                                                                       >  1:1172616/1‑140 (MQ=255)

                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                                                                     >  1:1100529/3‑137 (MQ=255)

                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                                                                     <  2:1100506/135‑1 (MQ=255)

                                    taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                                                                     <  2:1732100/135‑1 (MQ=255)

                                    tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                                                                  >  1:1869721/1‑140 (MQ=255)

                                    tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                                                                  >  2:252134/1‑140 (MQ=255)

                                    tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                                                                  >  2:643938/1‑140 (MQ=255)

                                    tGATTTCGCAATCCTCTGTAATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                                                                  >  2:643289/1‑140 (MQ=255)

                                      aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTGTGCTAAAACCGAAGTGTCTATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                                                                                <  2:2150867/140‑1 (MQ=255)

                                      aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                                                                                <  1:1387654/140‑1 (MQ=255)

                                        ttCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgcc                                                                                              <  2:1508202/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1167956/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1150927/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:201373/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1521409/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1840446/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1905963/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:1097394/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:525481/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:930085/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:901668/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:1597092/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:1344956/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:1938209/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:1962424/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:2251963/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:1252592/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:1250408/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:338498/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:543699/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:621220/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:735940/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:1036013/140‑1 (MQ=255)

                                           gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAACCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:2041335/140‑1 (MQ=255)

                                           gCAATCCTCTGTCAGGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1387133/140‑1 (MQ=255)

                                           gCAAGCCTCTGTCCTGCCTTCGAATCCTGCGACACTGACAATTTGTCCGGGCTCAAGCGGGTCTGCTACAACCGCACGGTCGATATCAGTGATCCGGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  1:364248/140‑1 (MQ=255)

                                           acAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1553255/139‑1 (MQ=255)

                                           acAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATATTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1800442/139‑1 (MQ=255)

                                                ccTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGta                                                                                      <  1:1487620/140‑1 (MQ=255)

                                                  tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                                                                    <  2:546208/140‑1 (MQ=255)

                                                  tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                                                                    <  2:1033799/140‑1 (MQ=255)

                                                  tcggTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                                                                    <  2:1263233/137‑1 (MQ=255)

                                                    tctCAAGCCTTTTAATCCTGCGAAAATCACAATTTTTCCTTGCTAAAGCTCGTCTGCTAAAAACGAAGTGTCGATATCTGTGAGACTGGCGTTTCCCTGAACGGAATCAGTACGGATTCCCTCCTGCCAGCCATTCTAgc                                                                                  >  2:1315824/3‑139 (MQ=255)

                                                       cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                                                               <  1:217889/140‑1 (MQ=255)

                                                       cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                                                               <  2:327059/140‑1 (MQ=255)

                                                       cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                                                               <  1:1803934/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGTGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  2:2427346/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCCGTATAGGAcaca                                                                             >  2:1955071/1‑140 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             >  1:98488/1‑140 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  1:970736/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  1:317035/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  1:970725/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             >  1:1889990/1‑140 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  2:1421242/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  2:42923/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             >  1:2353328/1‑140 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             >  1:161585/1‑140 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATTTTTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  2:1181353/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAATATTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGGGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  1:1466889/140‑1 (MQ=255)

                                                         tGCCTTGGAATCCTGCGACAATGACAAGTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  2:2424233/140‑1 (MQ=255)

                                                         ggCCTTGGAATCCTGCGACAATGACAATTTGTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                             <  2:1573805/139‑1 (MQ=255)

                                                              tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTa                                                                                                              <  2:352696/102‑1 (MQ=255)

                                                              tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTc                                                                        <  1:415307/140‑1 (MQ=255)

                                                                gAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCAt                                                                      <  1:1591642/140‑1 (MQ=255)

                                                                     cTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt                                                                 >  1:742366/1‑140 (MQ=255)

                                                                      tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:2333874/113‑1 (MQ=255)

                                                                      tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           <  2:1352078/113‑1 (MQ=255)

                                                                      tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                           >  1:1352078/1‑113 (MQ=255)

                                                                        cGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCtttt                                                              >  2:965012/1‑140 (MQ=255)

                                                                        cGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCtttt                                                              >  2:1612345/1‑140 (MQ=255)

                                                                                                 aaGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAgg                                     <  1:261065/140‑1 (MQ=255)

                                                                                                 aaGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAgg                                     <  1:927589/140‑1 (MQ=255)

                                                                                                 aaGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGGACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAgg                                     <  2:736216/140‑1 (MQ=255)

                                                                                                                aaCCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCt                      <  2:2147983/140‑1 (MQ=255)

                                                                                                                          tCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGc            >  2:877631/1‑140 (MQ=255)

                                                                                                                             atatCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCcag         <  2:915434/140‑1 (MQ=255)

                                                                                                                                 cTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCagcagc     <  2:893741/140‑1 (MQ=255)

                                                                                                                                    tgATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCata  >  1:855041/1‑140 (MQ=255)

CTGCAGTTGTATCTGAACCGCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATA  >  NZ_CP034943/2720697‑2720968

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`