BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,720,854	T→C	5.5%	E105E (GAA→GAG)	EO946_RS14065 ←	aspartate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,720,854	0	T	C	5.5%	573.0 / 14.4	180	E105E (GAA→GAG)	EO946_RS14065	aspartate kinase
Reads supporting (aligned to +/- strand): ref base T (58/111); new base C (3/7); total (62/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

Only 100 of 182 total aligned reads displayed.

GCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCATTTCTCGTCTGCTCGGC  >  NZ_CP034943/2720716‑2720989

 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACCATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCTAAGTCTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                                       >  1:168871/1‑140 (MQ=255)

 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGTACGGCTTc                                                                                                                                       >  1:2400214/1‑140 (MQ=255)

 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                                       >  2:735856/1‑140 (MQ=255)

 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                                       >  1:1952868/1‑140 (MQ=255)

 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                                       >  1:385119/1‑140 (MQ=255)

 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                                       >  1:385110/1‑140 (MQ=255)

 ccccGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTc                                                                                                                                       >  2:1814061/1‑140 (MQ=255)

      gCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTAc                                                                                                                                  >  1:255450/1‑140 (MQ=255)

            tGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTc                                                                                                                            >  1:1172616/1‑140 (MQ=255)

                 taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                                                                                          <  2:1100506/135‑1 (MQ=255)

                 taaTTTCACAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTccc                                                                                                                          >  1:1100506/3‑137 (MQ=255)

                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                                                                                       >  2:2074692/1‑140 (MQ=255)

                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                                                                                       >  2:643938/1‑140 (MQ=255)

                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                                                                                       >  2:252134/1‑140 (MQ=255)

                 tGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGcc                                                                                                                       >  1:1869721/1‑140 (MQ=255)

                   aTTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTg                                                                                                                     <  2:1096558/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:911943/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:543699/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:621220/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:735856/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:735940/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:809655/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:2431394/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:201373/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:998770/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:1150927/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:2226977/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:1521409/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:1905963/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:1250408/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:2147186/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:487463/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:1288901/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:1036013/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:525481/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:556716/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:135542/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:200910/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:1962424/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  1:1938209/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:930085/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAACCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:2041335/140‑1 (MQ=255)

                        gCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:1512703/140‑1 (MQ=255)

                        gCAATCCTCTGTCAGGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:1387133/140‑1 (MQ=255)

                        acAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATTTTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:1553255/139‑1 (MQ=255)

                        acAATCCTCTGTCATGCCTTGGAATCCTGCAACAATGACAATATTTCCTTTTTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTAATTCTGGCGTTTCCGTGAATGGCCTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:1800442/139‑1 (MQ=255)

                          aaTCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGCACGGCTTCCGTACGGATTCCCGCCTgccagcca                                                                                                              <  2:1174797/140‑1 (MQ=255)

                          aaTCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagcca                                                                                                              <  2:1359046/140‑1 (MQ=255)

                          aaTCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagcca                                                                                                              <  2:1182687/140‑1 (MQ=255)

                             ccTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGta                                                                                                           <  1:1487620/140‑1 (MQ=255)

                             ccTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGta                                                                                                           <  2:1191738/140‑1 (MQ=255)

                               tctGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGtata                                                                                                         <  2:1704917/140‑1 (MQ=255)

                                 tctCAAGCCTTTTAATCCTGCGAAAATCACAATTTTTCCTTGCTAAAGCTCGTCTGCTAAAAACGAAGTGTCGATATCTGTGAGACTGGCGTTTCCCTGAACGGAATCAGTACGGATTCCCTCCTGCCAGCCATTCTAgc                                                                                                       >  2:1315824/3‑139 (MQ=255)

                                    cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                                                                                    <  2:327059/140‑1 (MQ=255)

                                    cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                                                                                    <  1:972804/140‑1 (MQ=255)

                                    cATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGaca                                                                                                    <  1:1803934/140‑1 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  2:825764/140‑1 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  2:42923/140‑1 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  1:2364405/140‑1 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  2:1421242/140‑1 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  1:317035/140‑1 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  >  1:110996/1‑140 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  1:970725/140‑1 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  >  1:1889990/1‑140 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  >  1:1668125/1‑140 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAATATTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGGGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  1:1466889/140‑1 (MQ=255)

                                      tGCCTTGGAATCCTGCGACAATGACAAGTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  2:2424233/140‑1 (MQ=255)

                                      ggCCTTGGAATCCTGCGACAATGACAATTTGTCCGTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGAcaca                                                                                                  <  2:1573805/139‑1 (MQ=255)

                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTa                                                                                                                                   <  2:352696/102‑1 (MQ=255)

                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTa                                                                                                                                   >  1:352696/1‑102 (MQ=255)

                                           tGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTc                                                                                             <  1:415307/140‑1 (MQ=255)

                                             gAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCAt                                                                                           <  1:1591642/140‑1 (MQ=255)

                                                  cTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt                                                                                      >  1:1609847/1‑140 (MQ=255)

                                                  cTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt                                                                                      >  1:742366/1‑140 (MQ=255)

                                                   tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                <  2:2333874/113‑1 (MQ=255)

                                                   tGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTgccagc                                                                                                                >  1:2333874/1‑113 (MQ=255)

                                                     cGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCtttt                                                                                   >  2:965012/1‑140 (MQ=255)

                                                     cGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCtttt                                                                                   >  2:1612345/1‑140 (MQ=255)

                                                          atgacaatTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTg                                                                              >  2:1347378/1‑140 (MQ=255)

                                                                              aaGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAgg                                                          <  1:927589/140‑1 (MQ=255)

                                                                              aaGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAgg                                                          <  1:1347378/140‑1 (MQ=255)

                                                                              aaGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGGACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAgg                                                          <  2:736216/140‑1 (MQ=255)

                                                                                             aaCCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCt                                           <  2:2147983/140‑1 (MQ=255)

                                                                                             aaCCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCt                                           <  2:63060/140‑1 (MQ=255)

                                                                                                   aGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCg                                     >  1:274872/1‑140 (MQ=255)

                                                                                                   aGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCg                                     >  1:1413511/1‑140 (MQ=255)

                                                                                                         gATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCAGAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCca                               <  2:1945652/140‑1 (MQ=255)

                                                                                                          atatCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCcag                              <  2:915434/140‑1 (MQ=255)

                                                                                                              cTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCagcagc                          <  2:893741/140‑1 (MQ=255)

                                                                                                                 tgATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCata                       >  1:919683/1‑140 (MQ=255)

                                                                                                                 tgATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCata                       >  1:2214445/1‑140 (MQ=255)

                                                                                                                        ggCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCt                                                                                      >  1:1381314/1‑70 (MQ=255)

                                                                                                                        ggCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCAttt                <  1:101191/140‑1 (MQ=255)

                                                                                                                        ggCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCAttt                <  1:2131521/140‑1 (MQ=255)

                                                                                                                         gCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCATTtc               >  1:208331/1‑140 (MQ=255)

                                                                                                                         gCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCATTtc               >  1:616346/1‑140 (MQ=255)

                                                                                                                         gCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCATTtc               >  1:897093/1‑140 (MQ=255)

                                                                                                                                     aCGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCATTTCTCGTCTGCTCgg   <  1:2038318/140‑1 (MQ=255)

                                                                                                                                     aCGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCATTTCTCGTCTGCTCgg   <  1:1190387/140‑1 (MQ=255)

                                                                                                                                      cGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCATTTCTCGTCTGCTCGGc  <  1:2254016/140‑1 (MQ=255)

GCCCCGGCCCAATGTCGTGATTTCGCAATCCTCTGTCATGCCTTGGAATCCTGCGACAATGACAATTTTTCCTTGCTCAAGCTGGTCTGCTAAAACCGAAGTGTCGATATCTGTGATCCTGGCGTTTCCGTGAACGGCTTCCGTACGGATTCCCGCCTGCCAGCCAGTATAGGACACAGCGTCATAGCCTTTTTCCTGCAGCGCCATTGAAAGCAAGGAAATGGTGACCTGCTCGCCCGTCGCCAGCAGCATATCCATTTCTCGTCTGCTCGGC  >  NZ_CP034943/2720716‑2720989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`