Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,794,219 A→C 5.1% intergenic (‑101/+64) icd ← / ← citZ NADP‑dependent isocitrate dehydrogenase/citrate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,794,2190AC 5.1% 418.9 / 7.0 137intergenic (‑101/+64)icd/citZNADP‑dependent isocitrate dehydrogenase/citrate synthase
Reads supporting (aligned to +/- strand):  ref base A (67/60);  new base C (4/4);  total (73/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 147 total aligned reads displayed.

ACTCCATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGTCGG  >  NZ_CP034943/2794081‑2794358
                                                                                                                                          |                                                                                                                                           
aCTCCATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAAc                                                                                                                                            <  1:1189060/140‑1 (MQ=255)
aCTCCATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAAc                                                                                                                                            <  1:2425967/140‑1 (MQ=255)
aCTCCATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAAc                                                                                                                                            <  1:645552/140‑1 (MQ=255)
aCTCCATTACAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCACAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAAc                                                                                                                                            <  1:1946162/140‑1 (MQ=255)
    cATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACt                                                                                                                                        >  1:1164175/1‑140 (MQ=255)
    cATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACt                                                                                                                                        >  1:2277035/1‑140 (MQ=255)
    cATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACt                                                                                                                                        >  1:557237/1‑140 (MQ=255)
    cATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACt                                                                                                                                        >  1:751422/1‑140 (MQ=255)
    cATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAAAATTCAATGTAAAGTGTAAACCACt                                                                                                                                        >  2:2025356/1‑140 (MQ=255)
            aCTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGACAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGt                                                                                                                                >  1:1240989/1‑140 (MQ=255)
            aCTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGt                                                                                                                                >  2:1618824/1‑140 (MQ=255)
            aCTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGt                                                                                                                                >  2:1032194/1‑140 (MQ=255)
            aCTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGt                                                                                                                                >  1:428786/1‑140 (MQ=255)
            aCTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGt                                                                                                                                >  1:313/1‑140 (MQ=255)
            aCTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGt                                                                                                                                >  1:1768386/1‑140 (MQ=255)
            aCTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGt                                                                                                                                >  1:229043/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCATTGTAAAGTGTAAGCCACTTTTAATGGTc                                                                                                                              >  2:1569622/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTATTGTTc                                                                                                                              >  1:1622678/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGtta                                                                                                                              >  2:248178/1‑139 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTc                                                                                                                              >  1:813863/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTc                                                                                                                              >  1:444067/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTc                                                                                                                              >  1:1153911/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTc                                                                                                                              >  2:191310/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTc                                                                                                                              >  1:1517416/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTc                                                                                                                              >  2:2376627/1‑140 (MQ=255)
              tGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTAGGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTACAGTGTAAACCACTTTGAATGTTc                                                                                                                              >  2:1700170/1‑140 (MQ=255)
               ttAATTTTTTCAACTTGTTCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCt                                                                                                                             <  2:2400238/139‑1 (MQ=255)
               gTAAGTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCt                                                                                                                             <  2:2207438/140‑1 (MQ=255)
                    tttttCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACa                                                                                                                        <  1:1750170/140‑1 (MQ=255)
                        tCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAagag                                                                                                                    >  2:1568098/1‑140 (MQ=255)
                        tCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAagag                                                                                                                    >  2:150370/1‑140 (MQ=255)
                        tCACCTTGTGCCACAATAAAAACCTCCCAGTACGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAagag                                                                                                                    >  2:221455/1‑140 (MQ=255)
                           ccTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCt                                                                                                                 <  1:1871918/140‑1 (MQ=255)
                           ccTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCt                                                                                                                 <  1:309669/140‑1 (MQ=255)
                           ccTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAAAATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCt                                                                                                                 <  2:1856294/140‑1 (MQ=255)
                           ccTAGTGCCACAATAACAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCt                                                                                                                 <  1:1871917/140‑1 (MQ=255)
                                tgCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCt                                                                                                            >  1:345670/1‑140 (MQ=255)
                                   cacaATAAAAACCTCCCAGTATTTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCAt                                                                                                         <  2:2291596/140‑1 (MQ=255)
                                   cacaATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCAt                                                                                                         <  2:1342261/140‑1 (MQ=255)
                                   cacaATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCAt                                                                                                         <  1:954533/140‑1 (MQ=255)
                                   cacaATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCAt                                                                                                         <  2:383026/140‑1 (MQ=255)
                                   cacaATAAAAACCTCCCACTATGTAAGTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCAt                                                                                                         <  2:631383/140‑1 (MQ=255)
                                       aTAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAAc                                                                                                     >  2:2043812/1‑140 (MQ=255)
                                               cTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAagcca                                                                                             <  2:1517320/140‑1 (MQ=255)
                                               cTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAagcca                                                                                             <  2:373274/140‑1 (MQ=255)
                                                tCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAagccag                                                                                            >  1:2074802/1‑140 (MQ=255)
                                                  ccAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAagccagcc                                                                                          >  2:1134621/1‑140 (MQ=255)
                                                  ccAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAAACACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAagccagcc                                                                                          >  2:14624/1‑140 (MQ=255)
                                                        tGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAAt                                                                                    <  1:881235/140‑1 (MQ=255)
                                                         gTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATg                                                                                   <  1:1355103/140‑1 (MQ=255)
                                                         gTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATg                                                                                   <  2:796353/140‑1 (MQ=255)
                                                            aTTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGtt                                                                                >  1:2005940/1‑140 (MQ=255)
                                                                atatTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCttt                                                                            >  1:755047/1‑140 (MQ=255)
                                                                atatTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCttt                                                                            >  1:891795/1‑140 (MQ=255)
                                                                atatTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCttt                                                                            >  2:45949/1‑140 (MQ=255)
                                                                atatTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCttt                                                                            >  1:1196033/1‑140 (MQ=255)
                                                                atatTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCttt                                                                            >  1:1195647/1‑140 (MQ=255)
                                                                atatTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGACAGCCTCCAATGGTTCttt                                                                            >  1:2366498/1‑140 (MQ=255)
                                                                  atTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTTAACAAGAGGCTAACCTAATTAACGCAATCCATCCTCCAATGGTTCTTTAg                                                                          >  1:337035/1‑140 (MQ=255)
                                                                  atTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAg                                                                          >  1:2148562/1‑140 (MQ=255)
                                                                  atTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAg                                                                          >  1:1971141/1‑140 (MQ=255)
                                                                     tAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGct                                                                       <  2:657949/140‑1 (MQ=255)
                                                                           aCATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCtctttct                                                                 <  2:2148562/140‑1 (MQ=255)
                                                                           aCATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCtctttct                                                                 <  2:2148538/140‑1 (MQ=255)
                                                                                ttatttgTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAAt                                                            <  1:2165591/140‑1 (MQ=255)
                                                                                 tattttTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAAtt                                                           <  1:2376627/140‑1 (MQ=255)
                                                                                    ttttCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGa                                                        <  1:1646579/140‑1 (MQ=255)
                                                                                                gAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGt                                            <  2:1971141/140‑1 (MQ=255)
                                                                                                   aataaaaTGATTTTTCCTAACATTCAATTTAAAGTGTAACCCAATTTTGATGTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTAt                                        >  1:549421/4‑140 (MQ=255)
                                                                                                   aataaaaTGATTTTTCCTAACATTCAATTTAAAGTGTAACCCAATTTTGATGTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTAt                                        >  1:189220/4‑140 (MQ=255)
                                                                                                     cAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATc                                       <  2:2074802/140‑1 (MQ=255)
                                                                                                        aaTGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAgg                                    >  1:437508/1‑140 (MQ=255)
                                                                                                        aaTGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAgg                                    >  2:767536/1‑140 (MQ=255)
                                                                                                        aaTGATTTTTCCTAACATTCAATTTAAAGTGTAACCCAATTTTGATGTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAggg                                   <  2:549421/140‑1 (MQ=255)
                                                                                                        aaTGATTTTTCCTAACATTCAATTTAAAGTGTAACCCAATTTTGATGTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAggg                                   <  2:503404/140‑1 (MQ=255)
                                                                                                        aaTGATTTTTCCTAACATTCAATTTAAAGTGTAACCCAATTTTGATGTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAggg                                   <  2:189220/140‑1 (MQ=255)
                                                                                                         aTGATTTTTCTCAACATTCCATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAggg                                   <  2:1221575/140‑1 (MQ=255)
                                                                                                         aTGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAggg                                   <  1:1568098/140‑1 (MQ=255)
                                                                                                         aTGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAggg                                   <  1:221455/140‑1 (MQ=255)
                                                                                                         aTGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTGAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAggg                                   <  1:148927/140‑1 (MQ=255)
                                                                                                          tGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGc                                  <  2:751422/140‑1 (MQ=255)
                                                                                                          tGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGc                                  <  2:650689/140‑1 (MQ=255)
                                                                                                          tGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGc                                  <  1:1416322/140‑1 (MQ=255)
                                                                                                          tGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGc                                  <  1:950011/140‑1 (MQ=255)
                                                                                                          tGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGc                                  <  2:740461/140‑1 (MQ=255)
                                                                                                                          tCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGACCAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCcg                  >  2:1414263/1‑140 (MQ=255)
                                                                                                                             aTGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCgcgg               >  2:1799555/1‑140 (MQ=255)
                                                                                                                              tGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCgcggg              <  1:22444/140‑1 (MQ=255)
                                                                                                                              tGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCgcggg              <  1:248178/140‑1 (MQ=255)
                                                                                                                                 aaaGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCgcgggcgg           >  2:1717367/1‑140 (MQ=255)
                                                                                                                                       tAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGt     >  1:606645/1‑140 (MQ=255)
                                                                                                                                       tAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGt     <  1:1948248/140‑1 (MQ=255)
                                                                                                                                       tAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGt     >  1:409666/1‑140 (MQ=255)
                                                                                                                                        aaaCCACTTTTAATGTTCTGAACAAGAGGCTAACCTCCTTAAAGCAATCCAGACTCCAATTTTTCTTAAGTCTCTTTCTTCAAATGGAACAAATGCTTCGTTATCAGGGCCTGTGTAATGTGCCCGCGGGCGGATCAGTc    >  2:1176888/1‑140 (MQ=255)
                                                                                                                                        aaaCCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGTc    >  2:272804/1‑140 (MQ=255)
                                                                                                                                        aaaCCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGTc    >  2:1345043/1‑140 (MQ=255)
                                                                                                                                        aaaCCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGTc    >  2:898192/1‑140 (MQ=255)
                                                                                                                                        aaaCCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGTc    >  2:939195/1‑140 (MQ=255)
                                                                                                                                        aaaCCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTAGCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGTc    >  1:2416864/1‑140 (MQ=255)
                                                                                                                                          aCCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCgcggg              >  1:210397/1‑128 (MQ=255)
                                                                                                                                          |                                                                                                                                           
ACTCCATTAGAAACTGTAATTTTTTCACCTTGTGCCACAATAAAAACCTCCCAGTATGTAATTCATATTTAGAAAACATTTTATTTTTCCTATATAGAAAACAAAATGATTTTTCTCAACATTCAATGTAAAGTGTAAACCACTTTTAATGTTCTGAACAAGAGGCTAACCTCATTAACGCAAGCCAGCCTCCAATGGTTCTTTAGGCTCTTTCTTCAATTGGAACAAATGCTTGTTTATCAGGGCCTGTGTAATCTGCCCGCGGGCGGATCAGTCGG  >  NZ_CP034943/2794081‑2794358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: