Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,827,922 T→C 7.0% R329R (CGA→CGG thiI ← tRNA 4‑thiouridine(8) synthase ThiI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,827,9220TC 7.0% 352.2 / 12.1 114R329R (CGA→CGGthiItRNA 4‑thiouridine(8) synthase ThiI
Reads supporting (aligned to +/- strand):  ref base T (43/62);  new base C (4/4);  total (48/66)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 114 total aligned reads displayed.

TACGGCTCAAAATCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAAT  >  NZ_CP034943/2827788‑2828056
                                                                                                                                      |                                                                                                                                      
taCGGCTCAAAATCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTGATATGTGCCGAGGTCTCGTGa                                                                                                                                   >  2:1384757/1‑140 (MQ=255)
taCGGCTCAAAATCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGa                                                                                                                                   >  2:950280/1‑140 (MQ=255)
taCGGCTCAAAATCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCGCGTgt                                                                                                                                   >  2:1573880/1‑139 (MQ=255)
   ggCTCAAAATCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGAttt                                                                                                                                >  1:789514/1‑140 (MQ=255)
   ggCTCAAAATCAACAAAGCTTTCAAAGTGTTCGATGTTTTCTTTGTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGAttt                                                                                                                                >  1:541065/1‑140 (MQ=255)
    gCTCAAAATCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATCTCTCGTGAtttt                                                                                                                               >  2:1773952/1‑140 (MQ=255)
         aaaTCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCgat                                                                                                                          <  2:2046916/140‑1 (MQ=255)
         aaaTCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCgat                                                                                                                          <  2:1564867/140‑1 (MQ=255)
          aaTCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGTCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCgatg                                                                                                                         >  2:204596/1‑140 (MQ=255)
          aaTCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCgatg                                                                                                                         >  2:852888/1‑140 (MQ=255)
          aaTCAACAAAGCTTTCAAAGTGTTCGATTTTATCTTTTTTAGGGCGTGTTTTCTGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCgatg                                                                                                                         >  2:2310969/1‑140 (MQ=255)
             caacaaAGCTTTCAACGTGTTCGATTTTTTCTTTTTTAGGGCGTGATTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGAtt                                                                                                                      <  2:1117162/140‑1 (MQ=255)
              aacaaAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCGGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGAttt                                                                                                                     <  1:2287193/140‑1 (MQ=255)
              aacaaAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGAttt                                                                                                                     <  1:1525595/140‑1 (MQ=255)
              aacaaAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGAttt                                                                                                                     <  1:1688740/140‑1 (MQ=255)
              aacaaAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGAttt                                                                                                                     <  2:774954/140‑1 (MQ=255)
              aacaaAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGAttt                                                                                                                     <  1:2175220/140‑1 (MQ=255)
              aacaaAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGAttt                                                                                                                     <  2:628937/140‑1 (MQ=255)
              aacaaAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGAttt                                                                                                                     <  1:590071/140‑1 (MQ=255)
              aacaaAGATTTCAAAGTGTTCGTTTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTTGCTGAGTAAAGATCTTTCAGCAGTATTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGTTTTTTCGATGAttt                                                                                                                     <  1:2039067/140‑1 (MQ=255)
                   aGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGtt                                                                                                                <  2:901529/140‑1 (MQ=255)
                               gtTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCg                                                                                                    >  2:615560/1‑140 (MQ=255)
                                ttCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGTCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGATGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCTATGATTTCTTGTTTATCCATggata                                                                                                   >  2:925124/1‑137 (MQ=255)
                                ttCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCTATGATTTCTGTTTTATCAATGTCGa                                                                                                   >  1:246956/1‑140 (MQ=255)
                                                ttAGGGCGTGTTTTCGGGCTTGGCGGTGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATACTTGTCTCATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAAt                                                                                   <  2:2231170/140‑1 (MQ=255)
                                                ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                   <  2:1122449/140‑1 (MQ=255)
                                                ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                   <  1:1102452/140‑1 (MQ=255)
                                                ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                   <  2:1927845/140‑1 (MQ=255)
                                                ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                   <  1:1104167/140‑1 (MQ=255)
                                                ttAGGGCGTGTTGTCGGTCTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                   <  2:1926878/140‑1 (MQ=255)
                                                ttAGGGCGTGTGTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                   <  1:1102577/140‑1 (MQ=255)
                                                  tgggCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCg                                                                                 <  2:1281368/139‑1 (MQ=255)
                                                              cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                     <  1:2148712/140‑1 (MQ=255)
                                                              cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                     <  1:2030777/140‑1 (MQ=255)
                                                              cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                     <  1:2003758/140‑1 (MQ=255)
                                                              cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                     <  1:1266703/140‑1 (MQ=255)
                                                               ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                    <  1:615560/140‑1 (MQ=255)
                                                               ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                    <  2:204579/140‑1 (MQ=255)
                                                               ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                    <  2:2174526/140‑1 (MQ=255)
                                                                    tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                               >  1:109486/1‑140 (MQ=255)
                                                                    tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                               <  2:2332940/140‑1 (MQ=255)
                                                                    tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                               <  2:2332986/140‑1 (MQ=255)
                                                                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                           <  1:66062/140‑1 (MQ=255)
                                                                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                           <  2:1985002/140‑1 (MQ=255)
                                                                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                           <  1:1685837/140‑1 (MQ=255)
                                                                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                           <  1:852888/140‑1 (MQ=255)
                                                                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                           <  1:1747982/140‑1 (MQ=255)
                                                                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                           <  2:1370403/140‑1 (MQ=255)
                                                                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                           <  1:2094505/140‑1 (MQ=255)
                                                                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                           <  2:1370377/140‑1 (MQ=255)
                                                                                gATCGTGCAGGAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACa                                                   <  2:1586783/140‑1 (MQ=255)
                                                                                gATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACa                                                   <  2:1000298/140‑1 (MQ=255)
                                                                                 aTCGTGCAGCAGTCTTCAAAAGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACAt                                                  <  2:2095928/140‑1 (MQ=255)
                                                                                    gTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGCTTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGAGGTCACCGCATTAATCGACTACATGCt                                               >  1:2432209/1‑140 (MQ=255)
                                                                                              cTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGtt                                     <  1:1305539/140‑1 (MQ=255)
                                                                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                    >  2:739814/1‑140 (MQ=255)
                                                                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                    >  2:1225709/1‑140 (MQ=255)
                                                                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                    >  1:232356/1‑140 (MQ=255)
                                                                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                    >  1:2390799/1‑140 (MQ=255)
                                                                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                    >  2:1796670/1‑140 (MQ=255)
                                                                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                <  2:1816204/140‑1 (MQ=255)
                                                                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                <  2:2184324/140‑1 (MQ=255)
                                                                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                <  2:109486/140‑1 (MQ=255)
                                                                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                <  1:1243918/140‑1 (MQ=255)
                                                                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                <  1:1979111/140‑1 (MQ=255)
                                                                                                       gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                            >  2:70568/1‑140 (MQ=255)
                                                                                                       gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                            >  2:2258180/1‑140 (MQ=255)
                                                                                                        cTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTa                           >  1:7621/1‑140 (MQ=255)
                                                                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                          <  1:1505251/140‑1 (MQ=255)
                                                                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                          <  1:785304/140‑1 (MQ=255)
                                                                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                          <  2:214729/140‑1 (MQ=255)
                                                                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                          <  2:2204669/140‑1 (MQ=255)
                                                                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                          <  1:235783/140‑1 (MQ=255)
                                                                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                 >  1:1563888/1‑140 (MQ=255)
                                                                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                 >  1:2094463/1‑140 (MQ=255)
                                                                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                 >  1:2340860/1‑140 (MQ=255)
                                                                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                 >  1:547196/1‑140 (MQ=255)
                                                                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                 >  1:55332/1‑140 (MQ=255)
                                                                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                 >  1:1089957/1‑140 (MQ=255)
                                                                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACACGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                 >  1:2156647/1‑140 (MQ=255)
                                                                                                                     tcATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCt              <  1:1869805/140‑1 (MQ=255)
                                                                                                                     tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                            >  1:589598/1‑94 (MQ=255)
                                                                                                                     tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                            <  2:589598/94‑1 (MQ=255)
                                                                                                                       atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt            >  2:922046/1‑140 (MQ=255)
                                                                                                                       atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt            >  2:397372/1‑140 (MQ=255)
                                                                                                                       atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCGGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt            >  2:963491/1‑140 (MQ=255)
                                                                                                                       atatGTGCCGATGTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt            <  2:393603/140‑1 (MQ=255)
                                                                                                                        tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc           <  1:1961975/140‑1 (MQ=255)
                                                                                                                        tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc           <  1:1846187/140‑1 (MQ=255)
                                                                                                                        tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc           <  1:945585/140‑1 (MQ=255)
                                                                                                                        tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc           <  1:1118890/140‑1 (MQ=255)
                                                                                                                            tgCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTccccc       >  2:1656692/1‑140 (MQ=255)
                                                                                                                            tgCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACATGACCAAGGCTTTccccc       >  2:1656216/1‑140 (MQ=255)
                                                                                                                              ccaaTTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTAATTGCATAc                                                    <  2:357620/90‑1 (MQ=255)
                                                                                                                              ccGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGt     >  2:920628/1‑140 (MQ=255)
                                                                                                                                 aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat  >  1:317987/1‑140 (MQ=255)
                                                                                                                                 aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat  >  2:896688/1‑140 (MQ=255)
                                                                                                                                 aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAGCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat  >  1:883499/1‑140 (MQ=255)
                                                                                                                                   ttCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt            <  1:2299753/128‑1 (MQ=255)
                                                                                                                                   ttCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt            >  2:2299753/1‑128 (MQ=255)
                                                                                                                                      |                                                                                                                                      
TACGGCTCAAAATCAACAAAGCTTTCAAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAAT  >  NZ_CP034943/2827788‑2828056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: