Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,827,949 A→G 8.4% D320D (GAT→GAC thiI ← tRNA 4‑thiouridine(8) synthase ThiI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,827,9490AG 8.4% 287.4 / 12.3 95D320D (GAT→GACthiItRNA 4‑thiouridine(8) synthase ThiI
Reads supporting (aligned to +/- strand):  ref base A (35/52);  new base G (4/4);  total (39/56)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGC  >  NZ_CP034943/2827814‑2828062
                                                                                                                                       |                                                                                                                 
aaaGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCa                                                                                                               <  2:2094173/140‑1 (MQ=255)
     gtTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCg                                                                                                          >  2:615560/1‑140 (MQ=255)
      ttCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGTCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGATGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCTATGATTTCTTGTTTATCCATggata                                                                                                         >  2:925124/1‑137 (MQ=255)
      ttCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCTATGATTTCTGTTTTATCAATGTCGa                                                                                                         >  1:246956/1‑140 (MQ=255)
                      ttAGGGCGTGTTTTCGGGCTTGGCGGTGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATACTTGTCTCATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAAt                                                                                         <  2:2231170/140‑1 (MQ=255)
                      ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                         <  1:1102452/140‑1 (MQ=255)
                      ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                         <  1:1104167/140‑1 (MQ=255)
                      ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                         <  2:1927845/140‑1 (MQ=255)
                      ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                         <  2:1122449/140‑1 (MQ=255)
                      ttAGGGCGTGTTGTCGGTCTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                         <  2:1926878/140‑1 (MQ=255)
                      ttAGGGCGTGTGTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                         <  1:1102577/140‑1 (MQ=255)
                        tgggCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCg                                                                                       <  2:1281368/139‑1 (MQ=255)
                                    cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                           <  1:2148712/140‑1 (MQ=255)
                                    cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                           <  1:2030777/140‑1 (MQ=255)
                                    cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                           <  1:2003758/140‑1 (MQ=255)
                                    cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                           <  1:1266703/140‑1 (MQ=255)
                                     ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                          <  2:2174526/140‑1 (MQ=255)
                                     ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                          <  1:615560/140‑1 (MQ=255)
                                     ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                          <  2:204579/140‑1 (MQ=255)
                                          tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                                     <  2:2332986/140‑1 (MQ=255)
                                          tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                                     >  1:109486/1‑140 (MQ=255)
                                          tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                                     <  2:2332940/140‑1 (MQ=255)
                                              ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                 <  1:66062/140‑1 (MQ=255)
                                              ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                 <  1:852888/140‑1 (MQ=255)
                                              ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                 <  2:1985002/140‑1 (MQ=255)
                                              ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                 <  1:2094505/140‑1 (MQ=255)
                                              ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                 <  1:1747982/140‑1 (MQ=255)
                                              ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                 <  1:1685837/140‑1 (MQ=255)
                                              ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                 <  2:1370377/140‑1 (MQ=255)
                                              ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                 <  2:1370403/140‑1 (MQ=255)
                                                cgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCg                                                               <  2:1048728/140‑1 (MQ=255)
                                                      gATCGTGCAGGAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACa                                                         <  2:1586783/140‑1 (MQ=255)
                                                      gATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACa                                                         <  2:1000298/140‑1 (MQ=255)
                                                      gATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACa                                                         <  2:1428490/140‑1 (MQ=255)
                                                       aTCGTGCAGCAGTCTTCAAAAGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACAt                                                        <  2:2095928/140‑1 (MQ=255)
                                                          gTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGCTTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGAGGTCACCGCATTAATCGACTACATGCt                                                     >  1:2432209/1‑140 (MQ=255)
                                                                    cTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGtt                                           <  1:1305539/140‑1 (MQ=255)
                                                                     ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                          >  2:1796670/1‑140 (MQ=255)
                                                                     ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                          >  2:1225709/1‑140 (MQ=255)
                                                                     ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                          >  1:232356/1‑140 (MQ=255)
                                                                     ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                          >  1:2390799/1‑140 (MQ=255)
                                                                     ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                          >  2:739814/1‑140 (MQ=255)
                                                                         aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                      <  1:1243918/140‑1 (MQ=255)
                                                                         aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                      <  2:109486/140‑1 (MQ=255)
                                                                         aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                      <  1:1979111/140‑1 (MQ=255)
                                                                         aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                      <  2:2184324/140‑1 (MQ=255)
                                                                         aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                      <  2:1816204/140‑1 (MQ=255)
                                                                             gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGCTTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                  >  2:991938/1‑140 (MQ=255)
                                                                             gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                  >  2:2258180/1‑140 (MQ=255)
                                                                             gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                  >  2:517602/1‑140 (MQ=255)
                                                                             gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                  >  2:70568/1‑140 (MQ=255)
                                                                             gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                  >  2:891685/1‑140 (MQ=255)
                                                                              cTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTa                                 >  1:7621/1‑140 (MQ=255)
                                                                               tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                <  2:2204669/140‑1 (MQ=255)
                                                                               tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                <  1:785304/140‑1 (MQ=255)
                                                                               tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                <  1:1505251/140‑1 (MQ=255)
                                                                               tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                <  1:235783/140‑1 (MQ=255)
                                                                               tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                <  2:214729/140‑1 (MQ=255)
                                                                                        gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                       >  1:1089957/1‑140 (MQ=255)
                                                                                        gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                       >  1:1563888/1‑140 (MQ=255)
                                                                                        gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                       >  1:2094463/1‑140 (MQ=255)
                                                                                        gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                       >  1:2340860/1‑140 (MQ=255)
                                                                                        gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                       >  1:547196/1‑140 (MQ=255)
                                                                                        gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                       >  1:55332/1‑140 (MQ=255)
                                                                                        gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACACGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                       >  1:2156647/1‑140 (MQ=255)
                                                                                           tcATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCt                    <  1:1869805/140‑1 (MQ=255)
                                                                                           tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                                  <  2:589598/94‑1 (MQ=255)
                                                                                           tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                                  >  1:589598/1‑94 (MQ=255)
                                                                                           tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                                  >  1:1803333/1‑94 (MQ=255)
                                                                                           tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                                  <  2:1803333/94‑1 (MQ=255)
                                                                                             atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                  >  2:397372/1‑140 (MQ=255)
                                                                                             atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                  <  2:2259246/140‑1 (MQ=255)
                                                                                             atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                  >  2:922046/1‑140 (MQ=255)
                                                                                             atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCGGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                  >  2:963491/1‑140 (MQ=255)
                                                                                             atatGTGCCGATGTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                  <  2:393603/140‑1 (MQ=255)
                                                                                              tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc                 <  1:1846187/140‑1 (MQ=255)
                                                                                              tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc                 <  1:1118890/140‑1 (MQ=255)
                                                                                              tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc                 <  1:945585/140‑1 (MQ=255)
                                                                                              tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc                 <  1:1961975/140‑1 (MQ=255)
                                                                                                  tgCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTccccc             >  2:1656692/1‑140 (MQ=255)
                                                                                                  tgCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACATGACCAAGGCTTTccccc             >  2:1656216/1‑140 (MQ=255)
                                                                                                  tgCCGATTTCTCGGGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTccccc             >  1:687203/1‑140 (MQ=255)
                                                                                                    ccaaTTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTAATTGCATAc                                                          <  2:357620/90‑1 (MQ=255)
                                                                                                    ccaaTTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTAATTGCATAc                                                          >  1:357620/4‑93 (MQ=255)
                                                                                                    ccGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGt           >  2:920628/1‑140 (MQ=255)
                                                                                                       aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat        >  1:317987/1‑140 (MQ=255)
                                                                                                       aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat        >  1:1270629/1‑140 (MQ=255)
                                                                                                       aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat        >  2:896688/1‑140 (MQ=255)
                                                                                                       aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAGCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat        >  1:883499/1‑140 (MQ=255)
                                                                                                       aTTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTAATTGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCGAGGCTTTCTCCCGTAat        >  1:2312324/1‑140 (MQ=255)
                                                                                                         ttCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                  >  2:2299753/1‑128 (MQ=255)
                                                                                                         ttCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                  <  1:2299753/128‑1 (MQ=255)
                                                                                                             cGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCTTTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGc  <  1:1024603/140‑1 (MQ=255)
                                                                                                             cGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGc  <  1:2086330/140‑1 (MQ=255)
                                                                                                             cGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGc  <  1:468427/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                 
AAAGTGTTCGATTTTTTCTTTTTTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGC  >  NZ_CP034943/2827814‑2828062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: