Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,827,963 A→G 7.8% L316L (TTA→CTA)  thiI ← tRNA 4‑thiouridine(8) synthase ThiI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,827,9630AG 7.8% 313.1 / 12.4 102L316L (TTA→CTA) thiItRNA 4‑thiouridine(8) synthase ThiI
Reads supporting (aligned to +/- strand):  ref base A (42/52);  new base G (4/4);  total (46/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 102 total aligned reads displayed.

TTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGATTCTGTCGGCAAT  >  NZ_CP034943/2827836‑2828098
                                                                                                                               |                                                                                                                                       
ttAGGGCGTGTTTTCGGGCTTGGCGGTGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATACTTGTCTCATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAAt                                                                                                                             <  2:2231170/140‑1 (MQ=255)
ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                                                             <  1:1102452/140‑1 (MQ=255)
ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                                                             <  1:1104167/140‑1 (MQ=255)
ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                                                             <  2:1927845/140‑1 (MQ=255)
ttAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                                                             <  2:1122449/140‑1 (MQ=255)
ttAGGGCGTGTTGTCGGTCTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                                                             <  2:1926878/140‑1 (MQ=255)
ttAGGGCGTGTGTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAAt                                                                                                                             <  1:1102577/140‑1 (MQ=255)
  tgggCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCg                                                                                                                           <  2:1281368/139‑1 (MQ=255)
              cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                                                               <  1:2030777/140‑1 (MQ=255)
              cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                                                               <  1:2003758/140‑1 (MQ=255)
              cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                                                               <  1:2148712/140‑1 (MQ=255)
              cGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCa                                                                                                               <  1:1266703/140‑1 (MQ=255)
               ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                                                              <  1:615560/140‑1 (MQ=255)
               ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                                                              <  2:2174526/140‑1 (MQ=255)
               ggACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCAc                                                                                                              <  2:204579/140‑1 (MQ=255)
                    tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                                                                         <  2:2332940/140‑1 (MQ=255)
                    tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                                                                         <  2:2332986/140‑1 (MQ=255)
                    tGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCAt                                                                                                         >  1:109486/1‑140 (MQ=255)
                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                                                     <  1:66062/140‑1 (MQ=255)
                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                                                     <  2:1370377/140‑1 (MQ=255)
                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                                                     <  2:1370403/140‑1 (MQ=255)
                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                                                     <  1:2094505/140‑1 (MQ=255)
                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                                                     <  1:852888/140‑1 (MQ=255)
                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                                                     <  2:1985002/140‑1 (MQ=255)
                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                                                     <  1:1747982/140‑1 (MQ=255)
                        ggcgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAAt                                                                                                     <  1:1685837/140‑1 (MQ=255)
                          cgTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCg                                                                                                   <  2:1048728/140‑1 (MQ=255)
                                gATCGTGCAGGAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACa                                                                                             <  2:1586783/140‑1 (MQ=255)
                                gATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACa                                                                                             <  2:1428490/140‑1 (MQ=255)
                                gATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACa                                                                                             <  2:1000298/140‑1 (MQ=255)
                                 aTCGTGCAGCAGTCTTCAAAAGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACAt                                                                                            <  2:2095928/140‑1 (MQ=255)
                                    gTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGCTTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGAGGTCACCGCATTAATCGACTACATGCt                                                                                         >  1:2432209/1‑140 (MQ=255)
                                              cTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGtt                                                                               <  1:1305539/140‑1 (MQ=255)
                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                                                              >  2:1796670/1‑140 (MQ=255)
                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                                                              >  1:232356/1‑140 (MQ=255)
                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                                                              >  1:2390799/1‑140 (MQ=255)
                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                                                              >  2:739814/1‑140 (MQ=255)
                                               ttCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGttt                                                                              >  2:1225709/1‑140 (MQ=255)
                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                                                          <  2:109486/140‑1 (MQ=255)
                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                                                          <  1:1243918/140‑1 (MQ=255)
                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                                                          <  2:2184324/140‑1 (MQ=255)
                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                                                          <  2:1816204/140‑1 (MQ=255)
                                                   aaCGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCt                                                                          <  1:1979111/140‑1 (MQ=255)
                                                       gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                                                      >  2:70568/1‑140 (MQ=255)
                                                       gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                                                      >  2:2258180/1‑140 (MQ=255)
                                                       gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                                                      >  2:891685/1‑140 (MQ=255)
                                                       gCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCt                                                                      >  2:517602/1‑140 (MQ=255)
                                                        cTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTa                                                                     >  1:7621/1‑140 (MQ=255)
                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                                                    <  1:1505251/140‑1 (MQ=255)
                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                                                    <  2:2204669/140‑1 (MQ=255)
                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                                                    <  1:235783/140‑1 (MQ=255)
                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                                                    <  1:785304/140‑1 (MQ=255)
                                                         tGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTAc                                                                    <  2:214729/140‑1 (MQ=255)
                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                                                           >  1:1089957/1‑140 (MQ=255)
                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                                                           >  1:55332/1‑140 (MQ=255)
                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                                                           >  1:547196/1‑140 (MQ=255)
                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                                                           >  1:2340860/1‑140 (MQ=255)
                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                                                           >  1:2094463/1‑140 (MQ=255)
                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                                                           >  1:1563888/1‑140 (MQ=255)
                                                                  gTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACACGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAg                                                           >  1:2156647/1‑140 (MQ=255)
                                                                     tcATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCt                                                        <  1:1869805/140‑1 (MQ=255)
                                                                     tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                                                                      >  1:589598/1‑94 (MQ=255)
                                                                     tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                                                                      <  2:589598/94‑1 (MQ=255)
                                                                     tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                                                                      <  2:1803333/94‑1 (MQ=255)
                                                                     tcATATGTGCCAATTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTaa                                                                                                      >  1:1803333/1‑94 (MQ=255)
                                                                       atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                                                      <  2:2259246/140‑1 (MQ=255)
                                                                       atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                                                      >  2:397372/1‑140 (MQ=255)
                                                                       atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                                                      >  2:922046/1‑140 (MQ=255)
                                                                       atatGTGCCGATTTCTCGTGATTTTTCGATGATTTCGGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                                                      >  2:963491/1‑140 (MQ=255)
                                                                       atatGTGCCGATGTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                                                      <  2:393603/140‑1 (MQ=255)
                                                                        tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc                                                     <  1:945585/140‑1 (MQ=255)
                                                                        tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc                                                     <  1:1118890/140‑1 (MQ=255)
                                                                        tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc                                                     <  1:1846187/140‑1 (MQ=255)
                                                                        tatGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTc                                                     <  1:1961975/140‑1 (MQ=255)
                                                                            tgCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTccccc                                                 >  2:1656692/1‑140 (MQ=255)
                                                                            tgCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACATGACCAAGGCTTTccccc                                                 >  2:1656216/1‑140 (MQ=255)
                                                                            tgCCGATTTCTCGGGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTccccc                                                 >  1:687203/1‑140 (MQ=255)
                                                                              ccaaTTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTAATTGCATAc                                                                                              <  2:357620/90‑1 (MQ=255)
                                                                              ccaaTTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTAATTGCATAc                                                                                              >  1:357620/4‑93 (MQ=255)
                                                                              ccGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGt                                               >  2:920628/1‑140 (MQ=255)
                                                                                 aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat                                            >  2:896688/1‑140 (MQ=255)
                                                                                 aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat                                            >  1:1270629/1‑140 (MQ=255)
                                                                                 aTTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAat                                            >  1:317987/1‑140 (MQ=255)
                                                                                 aTTTCCCGTGATTTTTCGATGATTTCTGTTTTGTCCATGGCGATTAGCGGACGCAAAATCGGTGTTGACGTCACCGCATTAATTGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCGAGGCTTTCTCCCGTAat                                            >  1:2312324/1‑140 (MQ=255)
                                                                                   ttCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                                                      >  2:2299753/1‑128 (MQ=255)
                                                                                   ttCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCttt                                                      <  1:2299753/128‑1 (MQ=255)
                                                                                       cGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCTTTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGc                                      <  1:1024603/140‑1 (MQ=255)
                                                                                       cGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGc                                      <  1:2086330/140‑1 (MQ=255)
                                                                                       cGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGc                                      <  1:468427/140‑1 (MQ=255)
                                                                                                                  tCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCAtt                             >  1:336376/1‑122 (MQ=255)
                                                                                                                  tCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCAtt                             <  2:336376/122‑1 (MQ=255)
                                                                                                                  tCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGATTCt           >  2:663587/1‑140 (MQ=255)
                                                                                                                  tCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCAGTTCTCTTGTCTCTGAGTCt           >  2:1998013/1‑140 (MQ=255)
                                                                                                                       ggCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGATTCTGTCgg      >  1:2385171/1‑140 (MQ=255)
                                                                                                                       ggCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGATTCTGTCgg      >  2:962550/1‑140 (MQ=255)
                                                                                                                       ggCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGAGTCTGTCgg      >  1:1611020/1‑140 (MQ=255)
                                                                                                                       ggCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCAGTTCTCTTTTCTCTGATTCTGTCgg      >  1:1121203/1‑140 (MQ=255)
                                                                                                                       ggCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCACTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGATTCTGTCgg      >  2:476742/1‑140 (MQ=255)
                                                                                                                           aTTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGATTCTGTCGGCAAt  >  2:2319947/1‑140 (MQ=255)
                                                                                                                           aTTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGATTCTGTCGGCAAt  >  2:943892/1‑140 (MQ=255)
                                                                                                                               |                                                                                                                                       
TTAGGGCGTGTTTTCGGACTTGGCGGCGTAAAGATCGTGCAGCAGTCTTCAAACGGCTGAATGCTTGTCTCATATGTGCCGATTTCTCGTGATTTTTCGATGATTTCTGTTTTATCCATGGCGATTAACGGACGCAAAATCGGTGTTGACGTCACCGCATTAATCGCATACATGCTTTCAAGCGTTTGGCTCGCTACCTGACCAAGGCTTTCCCCCGTAATGATGGCAAGCCCATTTCTCTTTTCTCTGATTCTGTCGGCAAT  >  NZ_CP034943/2827836‑2828098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: