BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,837,054	A→T	7.1%	I122N (ATC→AAC)	EO946_RS14635 ←	diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,837,054	0	A	T	7.1%	370.6 / 14.7	112	I122N (ATC→AAC)	EO946_RS14635	diguanylate cyclase
Reads supporting (aligned to +/- strand): ref base A (44/60); new base T (4/4); total (48/64)
Fisher's exact test for biased strand distribution p-value = 7.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 115 total aligned reads displayed.

AATAAACAATGAAAACTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAGCCGTCCTCAATCATATAAAATG  >  NZ_CP034943/2836918‑2837189

aaTAAACAATGAAAACTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGatat                                                                                                                                      <  1:1704550/140‑1 (MQ=255)

     ataaagAAAACTCAAAAATATAAAATGTGATTGTCAAGCTTTCTAAAAGATGCTTCAGCACATCGGTCAGATCAAGCAGTGAATGGAACAGCTCTGTCATGTTCTATATCTTTTTTTGGTAGATCGTTTTGTTATAGAGt                                                                                                                                 >  1:915189/6‑140 (MQ=255)

     ataaagAAAACTCAAAAAGAGAAAATGTGCTTGTCAAGCTTTCTAAAAGCTGCTTCAGCACATCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGGTAGATCGTTTTGTTATAGAGt                                                                                                                                 >  1:311424/6‑140 (MQ=255)

     ataaagAAAACTCAAAAAGAGAAAATGTGCTTGTCAAGCTTTCTAAAAGCTGCTTCAGCACATCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGGTAGATCGTTTTGTTATAGAGt                                                                                                                                 >  1:855554/6‑140 (MQ=255)

     aCAATGAAAACTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGt                                                                                                                                 <  2:1704079/140‑1 (MQ=255)

        aTGAAAACTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCtt                                                                                                                              <  2:980545/140‑1 (MQ=255)

         tGAAAACTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCttt                                                                                                                             >  1:1770973/1‑140 (MQ=255)

         tGAAAACTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCttt                                                                                                                             >  1:1300980/1‑140 (MQ=255)

         tGAAAACTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCttt                                                                                                                             >  1:1301004/1‑140 (MQ=255)

          caaaaCTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTc                                                                                                                            <  1:782252/139‑1 (MQ=255)

                       gagaAACCGTGCGGGTCCAGCTTTCTACAAGCTGCTTCAGCACCTCTGTCTGATCCAGCAGTGAATGGAACAGCTCTGTCATTTTGTATCTCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGAc                                                                                                               <  2:1372813/140‑1 (MQ=255)

                       gagaAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGAc                                                                                                               <  2:1368090/140‑1 (MQ=255)

                       gagaAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGAc                                                                                                               <  2:73996/140‑1 (MQ=255)

                       gacaaaaCGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTATTTTTGATAGATCGTTTTGATATAGAGTCTTTCCGGTTTCAGTgtc                                                                                                               >  2:1069141/4‑138 (MQ=255)

                          aaaaCGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTgg                                                                                                            >  1:1077271/1‑140 (MQ=255)

                          aaaaCGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATAGAGAGTCTTTCCTGTTTCATTGACTgg                                                                                                            >  1:1828992/1‑140 (MQ=255)

                                 gCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATag                                                                                                                                    >  2:371787/1‑109 (MQ=255)

                                 gCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATag                                                                                                                                    <  1:371787/109‑1 (MQ=255)

                                 gCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGAc                                                                                                               >  2:1056796/1‑130 (MQ=255)

                                 gCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGAc                                                                                                               <  1:1056796/130‑1 (MQ=255)

                                 gCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTaaaa                                                                                                     >  2:1774178/1‑140 (MQ=255)

                                 gCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTaaaa                                                                                                     >  2:552098/1‑140 (MQ=255)

                                           cTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAAc                                                                                           <  2:74625/140‑1 (MQ=255)

                                             ttCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACATCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCa                                                                                         <  2:994602/140‑1 (MQ=255)

                                             ttCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCa                                                                                         <  2:2274687/140‑1 (MQ=255)

                                             ttCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCa                                                                                         <  2:2033397/140‑1 (MQ=255)

                                             ttCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCa                                                                                         <  2:1618067/140‑1 (MQ=255)

                                             ttCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCa                                                                                         <  2:1413351/140‑1 (MQ=255)

                                             ttCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCa                                                                                         <  2:1074773/140‑1 (MQ=255)

                                                tAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGt                                                                                      <  1:1104316/140‑1 (MQ=255)

                                                     gctgctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGaa                                                                                 <  1:424986/140‑1 (MQ=255)

                                                     gctgctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGaa                                                                                 <  2:1770973/140‑1 (MQ=255)

                                                     gctgctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGaa                                                                                 >  1:1787181/1‑140 (MQ=255)

                                                     gctgctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGaa                                                                                 <  2:1301004/140‑1 (MQ=255)

                                                     gctgctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGaa                                                                                 <  2:1300980/140‑1 (MQ=255)

                                                     gctgctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGaa                                                                                 >  1:2278576/1‑140 (MQ=255)

                                                     gctgctTCAGCACCTCTGTCGGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGCTAGATCGTTTTGATATCGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGaa                                                                                 <  1:759034/140‑1 (MQ=255)

                                                       tgctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAg                                                                               <  1:1139941/140‑1 (MQ=255)

                                                       tgctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAg                                                                               <  1:1507533/140‑1 (MQ=255)

                                                        gctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGatat                                                                                                                                      <  1:1192821/84‑1 (MQ=255)

                                                        gctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGatat                                                                                                                                      >  2:1192821/1‑84 (MQ=255)

                                                        gctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGc                                                                              >  2:244782/1‑140 (MQ=255)

                                                        gctTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGc                                                                              >  2:1862791/1‑140 (MQ=255)

                                                                aCCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAgg                                                                      <  2:455487/140‑1 (MQ=255)

                                                                aCCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAgg                                                                      <  2:2118940/140‑1 (MQ=255)

                                                                  ctctTTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGCTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGaa                                                                    <  2:2066550/140‑1 (MQ=255)

                                                                  ctctGTCTTATCAAGCTGTGAATGGAACAGCTCTGTCATTTTTTATATCTTTTTTGGATATATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGaa                                                                    <  2:1378155/140‑1 (MQ=255)

                                                                  ctctGTCTGATCAAGCAGTGAATGGACCAGCTCTGTCATTGTGTCTATCTTTTTTTGATAGATCGTTTTGATATAGCGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGaa                                                                    <  2:245412/140‑1 (MQ=255)

                                                                  ctctGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGaa                                                                    <  2:275870/140‑1 (MQ=255)

                                                                  ctctGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGaa                                                                    <  2:1787181/140‑1 (MQ=255)

                                                                  ctctGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGaa                                                                    <  2:1112637/140‑1 (MQ=255)

                                                                  ctctGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGaa                                                                    <  2:2278576/140‑1 (MQ=255)

                                                                  ctctGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGaa                                                                    <  2:2175524/140‑1 (MQ=255)

                                                                    ctgtctgATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAAtc                                                                  >  1:504765/1‑140 (MQ=255)

                                                                       tctgATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAAtctct                                                               >  1:684967/1‑140 (MQ=255)

                                                                       tctgATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGAGCGTTTTGATCTAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCTGGAAtctct                                                               >  1:1796418/1‑140 (MQ=255)

                                                                        ctgATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAAtctctc                                                              <  1:1061194/140‑1 (MQ=255)

                                                                           aTCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAc                                                           >  2:952481/1‑140 (MQ=255)

                                                                            tCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          <  1:1089925/140‑1 (MQ=255)

                                                                            tCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          >  1:410798/1‑140 (MQ=255)

                                                                            tCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          <  1:732228/140‑1 (MQ=255)

                                                                            tCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          >  2:587945/1‑140 (MQ=255)

                                                                            tCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          <  1:587945/140‑1 (MQ=255)

                                                                            tCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          >  2:732228/1‑140 (MQ=255)

                                                                            tCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          >  1:2410485/1‑140 (MQ=255)

                                                                            acAACCAGTGAATGGAACAGCTCTGACATTTTGTAGATCCTTTTTCGATAGATCGTATTGATATCGCGTCTTTCCGGTTTCATTGACTGGTTTACAAATGAAGCAACCACGTGCGCAAGCCCTTCAGGAATCTCTCCAcc                                                          <  1:1700283/139‑1 (MQ=255)

                                                                             cAAGGAGTGAATTGAACAGCTGTGTCATTTTGTATATATTTTTTTGGTAGATCGTTTTGTTATAGAGTCTTTCCTGTTTCATCGACTGGTTTAAAAATGAAGCAAGCACGTCCGGAAGCCCTTCAGGAATCTCTTCAtcc                                                         <  2:915189/140‑1 (MQ=255)

                                                                             cAAGCAGTGAATGGAACAGCTCTTTCATTTTGTATATCTTTTTTTGGTAGATCGTTTTGTTATAGAGTCTTTCCTGTTTCATCGACTGGTTTAAAAATGAAGCAACCACGTCCGGAAGCCCTTCAGGAATCTCTTCAtcc                                                         <  2:1768081/140‑1 (MQ=255)

                                                                             cAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGGTAGATCGTTTTGTTATAGAGTCTTTCCTGTTTCATCGACTGGTTTAAAAATGAAGCAACCACGTCCGGAAGCCCTTCAGGAATCTCTTCAtcc                                                         <  2:855554/140‑1 (MQ=255)

                                                                             cAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGGTAGATCGTTTTGTTATAGAGTCTTTCCTGTTTCATCGACTGGTTTAAAAATGAAGCAACCACGTCCGGAAGCCCTTCAGGAATCTCTTCAtcc                                                         <  2:311424/140‑1 (MQ=255)

                                                                              aaGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTTGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcccg                                                        <  2:335789/140‑2 (MQ=255)

                                                                              aaGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCt                                                        <  2:1317657/140‑1 (MQ=255)

                                                                               aGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGATTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTg                                                       >  1:2374421/1‑140 (MQ=255)

                                                                               aGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGa                                                                     >  2:2410171/1‑126 (MQ=255)

                                                                                      aaTGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGaa                                                                                                <  1:1205937/92‑1 (MQ=255)

                                                                                      aaTGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGaa                                                                                                >  2:1205937/1‑92 (MQ=255)

                                                                                      aaTGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          <  2:605847/130‑1 (MQ=255)

                                                                                      aaTGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCAcc                                                          >  1:605847/1‑130 (MQ=255)

                                                                                       aTGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATg                                               <  1:737214/140‑1 (MQ=255)

                                                                                         ggAACAGCTCTGTAATTTTTTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCATGTTTCATTGACTGGTTTAAAAATGAAGCAACCACTTCAGAAAGCCCTTCTTTAATCTATCCACCCTGTCTTCTTGTa                                             <  2:1002782/140‑1 (MQ=255)

                                                                                           aaCAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAg                                           >  2:739596/1‑140 (MQ=255)

                                                                                           aaCAGCTCTGTCAGTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAg                                           >  2:406495/1‑140 (MQ=255)

                                                                                                ctctGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGaa                                      >  1:805197/1‑140 (MQ=255)

                                                                                                ctctGTCATTGTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGaa                                      >  1:662192/1‑140 (MQ=255)

                                                                                                    gTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAgcg                                  <  2:1595902/140‑1 (MQ=255)

                                                                                                    gTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAgcg                                  <  2:2067416/140‑1 (MQ=255)

                                                                                                     tCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAgcgc                                 >  2:1897898/1‑140 (MQ=255)

                                                                                                     tCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAgcgc                                 >  1:1243591/1‑140 (MQ=255)

                                                                                                          ttGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGcc                            >  1:417746/1‑140 (MQ=255)

                                                                                                              atatCTTTTTTTTATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGATTAAAAATGAAGCAACCACGTCCGAACGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAg                        <  2:364388/140‑1 (MQ=255)

                                                                                                              atatCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTTTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATTAAAGCGCCTGCCGGAg                        <  2:856873/140‑1 (MQ=255)

                                                                                                              atatCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAg                        <  2:2296428/140‑1 (MQ=255)

                                                                                                              atatCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAg                        <  1:687784/140‑1 (MQ=255)

                                                                                                              atatCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAg                        <  2:20937/140‑1 (MQ=255)

                                                                                                              atatCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAg                        <  2:1610586/140‑1 (MQ=255)

                                                                                                                   tttttttGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAGCCGTc                   <  1:937840/140‑1 (MQ=255)

                                                                                                                                    tttGATATATAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAATCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAGCCGTCCTCAATCATATAAAATg  >  2:1558117/1‑140 (MQ=255)

                                                                                                                                    tttGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCAGGAGCCGTCCTCAATCATATAAAATg  >  2:2336663/1‑140 (MQ=255)

                                                                                                                                    tttGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGAAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAGCCGTCCTCAATCATATAAAATg  >  2:1558780/1‑140 (MQ=255)

                                                                                                                                    tgtGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAGCCGTCCTCAATCATATAAAATg  >  2:1558881/3‑140 (MQ=255)

AATAAACAATGAAAACTCAATAGGAGAAAACGTGCGGGTCAAGCTTTCTAAAAGCTGCTTCAGCACCTCTGTCTGATCAAGCAGTGAATGGAACAGCTCTGTCATTTTGTATATCTTTTTTTGATAGATCGTTTTGATATAGAGTCTTTCCTGTTTCATTGACTGGTTTAAAAATGAAGCAACCACGTCCGAAAGCCCTTCAGGAATCTCTCCACCCTGTCTGCATGTAAGATGAAAGCGCCTGCCGGAGCCGTCCTCAATCATATAAAATG  >  NZ_CP034943/2836918‑2837189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`