Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 438,594 C→T 5.6% E47E (GAG→GAA gabR ← transcriptional regulator GabR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943438,5940CT 5.6% 585.3 / 13.8 179E47E (GAG→GAAgabRtranscriptional regulator GabR
Reads supporting (aligned to +/- strand):  ref base C (62/105);  new base T (4/6);  total (66/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

ATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCA  >  NZ_CP034943/438458‑438733
                                                                                                                                        |                                                                                                                                           
aTGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCg                                                                                                                                          >  2:2345580/1‑140 (MQ=255)
   gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                       >  1:100111/3‑140 (MQ=255)
   gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                       >  1:1127422/3‑140 (MQ=255)
   gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                       >  1:621177/3‑140 (MQ=255)
   gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                       >  1:397235/3‑140 (MQ=255)
     gggTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAg                                                                                                                                     >  2:524815/1‑140 (MQ=255)
     gggTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAg                                                                                                                                     >  2:1193796/1‑140 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                   <  2:961335/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                   <  2:925159/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                   >  2:1346434/1‑140 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                   <  1:2335204/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                   <  1:1805021/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAAGGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                   <  1:2283995/140‑1 (MQ=255)
         gCTCCACGGCAGAAAACATGTCCAGCGTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATAGCCCGCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctct                                                                                                                                 <  1:698905/140‑1 (MQ=255)
          cTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctc                                                                                                                                >  1:1008180/1‑140 (MQ=255)
            ccccGGCAGAAAACATGTCCAGCTTCTCCACCAAACACCCCTTTCGCTCAATGGCGTACAAATAGCCCTCAGCCAGAAGCTGCTGATAGGCTGAATCCACTGAGTTTACGCGGCCCTTCAGCTTCTCGGCCAGctctctc                                                                                                                              <  2:2121566/137‑1 (MQ=255)
            ccACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctctc                                                                                                                              <  1:974258/140‑1 (MQ=255)
            ccACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctctc                                                                                                                              <  2:298003/140‑1 (MQ=255)
            ccACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctctc                                                                                                                              <  2:297729/140‑1 (MQ=255)
               cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                           <  2:891957/140‑1 (MQ=255)
               cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                           <  2:1635525/140‑1 (MQ=255)
                 gCAGAACAAATGTCCAGCTTCTCCCCGAAAACCCCCTGTCGCTCAATGGCGTACAAATATCCCTCACCCAGAAGCTGCCGCTAGGCTGAATTCACTGAGTTTACGCTGACCTGCAGCTTCTCGGCCAGCTCTCTCTTGGa                                                                                                                         <  2:501739/140‑1 (MQ=255)
                  cAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAg                                                                                                                        >  2:411574/1‑140 (MQ=255)
                   aGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAgg                                                                                                                       <  1:1392547/140‑1 (MQ=255)
                         cATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCt                                                                                                                 <  2:1481382/140‑1 (MQ=255)
                            gTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCg                                                                                                              <  2:1008180/140‑1 (MQ=255)
                            gTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCg                                                                                                              <  2:44127/140‑1 (MQ=255)
                               cAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAgt                                                                                                           >  1:2245631/1‑140 (MQ=255)
                                      tcCACGAACAACCCCTGTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACGGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                    <  2:1307484/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                    <  1:1042564/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                    <  2:1590603/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                    <  1:2118609/140‑1 (MQ=255)
                                           gAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCGTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGAt                                                                                               <  1:1650396/140‑1 (MQ=255)
                                                  cccTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCt                                                                                        >  1:2277529/1‑140 (MQ=255)
                                                       tCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGaa                                                                                   >  1:2081827/1‑140 (MQ=255)
                                                               tGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCtttt                                                                           >  2:124560/1‑140 (MQ=255)
                                                               tGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCtttt                                                                           >  1:589286/1‑140 (MQ=255)
                                                                  cGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAgg                                                                                                                       <  2:1466645/93‑1 (MQ=255)
                                                                    tACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAg                                                                      >  1:1070552/1‑140 (MQ=255)
                                                                    tACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAg                                                                      >  1:1566816/1‑140 (MQ=255)
                                                                    tACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAg                                                                      >  1:2036642/1‑140 (MQ=255)
                                                                         atatCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGttttt                                                                 >  1:763274/1‑140 (MQ=255)
                                                                         atatCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGttttt                                                                 >  1:1088496/1‑140 (MQ=255)
                                                                           atCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctc                                                                                                                                  <  1:1360008/73‑1 (MQ=255)
                                                                           atCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctc                                                                                                                                  >  2:1360008/1‑73 (MQ=255)
                                                                            tCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGAt                                                              >  2:951733/1‑140 (MQ=255)
                                                                            tCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGAt                                                              >  2:1369303/1‑140 (MQ=255)
                                                                                 cAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAAtt                                                         >  2:518340/1‑140 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCCCTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGCACCTTCGAGTGTGGCAGCAGATTACGGGTGAGAATTTGTTTTTTTATTTTTGGATAAATTTGCTga                                                   <  2:1503827/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                   <  2:1683597/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                   <  2:1795722/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                   <  2:2203670/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                   <  1:313707/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                   <  1:1385850/140‑1 (MQ=255)
                                                                                              ctgATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTTCTGATAGatat                                            >  1:1271684/1‑140 (MQ=255)
                                                                                              ctgATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGatat                                            >  1:1443168/1‑140 (MQ=255)
                                                                                              ctgATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGatat                                            >  1:1271727/1‑140 (MQ=255)
                                                                                                gacaGGCTGAATTCCCGGAGTTGACGCTGACCTTCAGCTTCTCGGCCAGCTCTCCCTTGGAGGGGACCGTCGAGGGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                          <  2:1445558/137‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                          <  2:2277541/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                          <  2:941325/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                          <  2:621444/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                          <  1:888229/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                          <  2:1846502/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCGGATAGATATaa                                          <  2:745751/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTGTTTGATAAATTTGCTGATAGAtatac                                          <  2:1017572/140‑2 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCGTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                          <  2:2019636/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTATCGGCCAGCTCTCTCTTGTATGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                          <  2:2029402/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGCGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  1:1469610/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  1:518340/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  1:1593134/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  1:430594/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  2:1717626/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  2:2245631/140‑1 (MQ=255)
                                                                                                     gCTGAATTCAATGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  2:1374339/140‑1 (MQ=255)
                                                                                                     gCTGAAGTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  1:1904574/140‑1 (MQ=255)
                                                                                                      cTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCCGCTCTCTCTTGGAGGGGACCTTCGCCTGTGGCAGCAGATTACGGCTGAGACTTTCTTTTTTTAGTTTTTGATAAATTTGCTGATCGATATAATCGGCt                                    >  2:1066710/1‑140 (MQ=255)
                                                                                                               gtGAGTTTACTCTTAGCTTGAGGTTATCGTCCTGTTTTGTTTTGGTGGGGAGCTTCGATTGTGGCAGAATATTACTGCTGTTAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGTTAGTTATATTCGGTTTGTTTTGAg                           <  2:2174474/139‑1 (MQ=255)
                                                                                                               cTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAg                           <  2:850278/140‑1 (MQ=255)
                                                                                                               cTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAg                           <  2:1392414/140‑1 (MQ=255)
                                                                                                                    tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                      >  1:1419545/1‑140 (MQ=255)
                                                                                                                    tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                      <  1:448435/140‑1 (MQ=255)
                                                                                                                    tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                      >  1:2081293/1‑140 (MQ=255)
                                                                                                                    tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                      <  1:2014945/140‑1 (MQ=255)
                                                                                                                    tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGGGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATAGTCTTGTTCTGAGCGGTc                      >  1:1234704/1‑140 (MQ=255)
                                                                                                                       ccGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTGGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg                   <  2:2133966/139‑1 (MQ=255)
                                                                                                                        cGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                     <  1:1737763/121‑1 (MQ=255)
                                                                                                                           tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta               <  2:621177/140‑2 (MQ=255)
                                                                                                                           tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta               <  2:397235/140‑2 (MQ=255)
                                                                                                                           tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta               <  2:1127422/140‑2 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa              >  2:1832870/1‑140 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa              >  1:1809630/1‑140 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa              >  1:2319835/1‑140 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa              <  1:256157/140‑1 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa              <  1:259773/140‑1 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa              <  1:889830/140‑1 (MQ=255)
                                                                                                                                 tCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAAtgtg         <  2:1962011/140‑1 (MQ=255)
                                                                                                                                       tctcGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTcc   >  1:1204551/1‑140 (MQ=255)
                                                                                                                                       tctcGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTcc   >  1:873303/1‑140 (MQ=255)
                                                                                                                                       tctcGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTcc   >  1:594880/1‑140 (MQ=255)
                                                                                                                                        ctcGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCa  >  2:2141747/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
ATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCA  >  NZ_CP034943/438458‑438733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: