Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,901,671 A→C 8.7% K411T (AAA→ACA)  EO946_RS14945 → extracellular solute‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,901,6710AC 8.7% 294.7 / 5.5 104K411T (AAA→ACA) EO946_RS14945extracellular solute‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (83/10);  new base C (2/9);  total (85/19)
Fisher's exact test for biased strand distribution p-value = 1.51e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

ATGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCT  >  NZ_CP034943/2901533‑2901804
                                                                                                                                           |                                                                                                                                     
     aaaGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACt                                                                                                                                 >  1:1178137/1‑140 (MQ=255)
     aaaGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACt                                                                                                                                 >  2:2231226/1‑140 (MQ=255)
     aaaGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACCCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACt                                                                                                                                 >  1:1073144/1‑140 (MQ=255)
     aaaGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGGGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACt                                                                                                                                 >  2:177292/1‑140 (MQ=255)
                ttACTTCGACGGTTTATACTGCCAAACCGGATTGTGGGTGGGCCTGGGCAGGGTCGGACTGGATTTTATGGATCAAATTTTCACGCCTAAAGATATTTCTAAGACGCTGGTAAGACAAAACAAACGACTCCGGGAGCAAt                                                                                                                      <  2:83385/140‑1 (MQ=255)
                  cctcccaCCTTTTATAGTTCCCACAGGTAATGTGGTTTGGGCGGGCCACGGTCGCACAGGCGTTTTGGGCTCAAATTTTCACGCCTAACGAGTTTGCCACCAAGGTGGGAATACAAACCACACGACTCCGGGAGCAATCg                                                                                                                    <  2:144795/134‑1 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGCGCAATCGGAcaca                                                                                                              >  1:1857140/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                              >  1:1043605/1‑140 (MQ=255)
                        aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                              >  1:716187/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGCGATTTCGAAGAAGCTGGGAAGAGACTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTcaac                                                                                                 >  2:1431772/1‑139 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCCATCGGAAACACAGGGAGCTgaag                                                                                                 >  1:1583494/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                 >  2:125384/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                 >  2:1409132/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                 >  1:531978/1‑140 (MQ=255)
                                     gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                 >  2:1030062/1‑140 (MQ=255)
                                     gCCAAACGGAATGGGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                 >  1:229875/1‑140 (MQ=255)
                                      ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                >  1:2330089/1‑140 (MQ=255)
                                          aCGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACaa                                                                                            >  1:2340252/1‑140 (MQ=255)
                                          aCGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACaa                                                                                            >  1:55749/1‑140 (MQ=255)
                                                 tgtgGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAAtgagtg                                                                                      >  1:1559743/1‑140 (MQ=255)
                                                 tgtgGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAAtgagtg                                                                                      >  1:555582/1‑140 (MQ=255)
                                                   tgtgttGTCCTGGGAAGGGTCGCACAGGAGTTTTTGGCTTAAATTTTCACGCATACAGAGATTTCCAAGAAGCTGGTAACATAAAACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAAtgagtgag                                                                                    <  2:1913242/135‑1 (MQ=255)
                                                   tgGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAAtgagtgag                                                                                    >  2:757885/1‑140 (MQ=255)
                                                   tgGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAAtgagtgag                                                                                    >  2:1252720/1‑140 (MQ=255)
                                                      gATGTCCTGCGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTCGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATc                                                                                 >  2:157880/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGACGCTGGGAAGAGAATACAAACGACTCCGGGAGCACTCGGACACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                              >  1:792594/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                              >  1:2034667/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                              >  1:2034641/1‑140 (MQ=255)
                                                           ccTGGGCACGGTCGGACCGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAAGGAGTGAGATCGCAag                                                                            >  1:754832/1‑140 (MQ=255)
                                                           ccTGGGCACGGTCGGACAGGATCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGATATCTCAag                                                                            >  1:550743/1‑140 (MQ=255)
                                                             tGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACCAACGACTCCGGGAGCAATCGGACACACAGGGAGCTGAAGAACACTGAGTGAGATCGCacgtg                                                                          >  2:1481340/1‑136 (MQ=255)
                                                              gggCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAgaga                                                                         >  1:769737/1‑140 (MQ=255)
                                                              gggCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAgaga                                                                         >  1:8785/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGACGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATAGCAAGTGAGGTTCa                                                                   >  1:2320512/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGACGAGAATACAAACGACTCCGGGAGCAATCGGAAACACCGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:1342609/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTct                                                                   >  1:1070930/1‑139 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:315487/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:1396860/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:405156/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:1622267/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:362029/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:1877348/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:343977/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:2004013/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:26840/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:750709/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:2161714/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:2186376/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:1920447/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:2191717/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  1:2322259/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:2261778/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                   >  2:1957521/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGAAAGAGACGTTCa                                                                   >  1:2125982/1‑140 (MQ=255)
                                                                     gTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAt                                                                  >  1:1284124/1‑140 (MQ=255)
                                                                     gTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAt                                                                  >  1:1340873/1‑140 (MQ=255)
                                                                              gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAg                                                         >  1:2363530/1‑140 (MQ=255)
                                                                              gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAg                                                         >  2:491617/1‑140 (MQ=255)
                                                                                gCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGt                                                       >  2:156067/1‑140 (MQ=255)
                                                                                gCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGt                                                       >  2:11903/1‑140 (MQ=255)
                                                                                gCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGt                                                       >  1:548589/1‑140 (MQ=255)
                                                                                gCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACCCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGt                                                       >  2:925792/1‑140 (MQ=255)
                                                                                    aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAAAACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                   >  2:320005/1‑140 (MQ=255)
                                                                                           aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                            >  2:659396/1‑140 (MQ=255)
                                                                                           aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                            >  2:1240622/1‑140 (MQ=255)
                                                                                           aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                            >  2:2184142/1‑140 (MQ=255)
                                                                                           aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                            >  2:200172/1‑140 (MQ=255)
                                                                                           aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                            >  2:194098/1‑140 (MQ=255)
                                                                                           aCAGTTTGACGCCTCAAGAGATTTCGACGAAGCTGGGAAGAGACTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCCAGAGAGGTTCATGTGAAACAGGTGAAGCCGagag                                            >  1:2135657/1‑137 (MQ=255)
                                                                                             tttttGCCGCCTCAATATATTTCTAACAAGGTGGTAAGAGAATAAAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAg                                          <  2:2330089/139‑1 (MQ=255)
                                                                                             tttttGACGCCTAAAGTTATTTCGAACAAGCTGGTAACATAAAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAg                                          <  2:2004013/139‑1 (MQ=255)
                                                                                             aTTTTACCGCCACAAGAGATTAGGATGACCGGGGAAAGAGAATAACCACGACTCCGGGATCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAg                                          <  2:1489508/140‑1 (MQ=255)
                                                                                                ttGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAg                                       >  1:1294926/1‑140 (MQ=255)
                                                                                                ttGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAg                                       >  1:1002623/1‑140 (MQ=255)
                                                                                                       cacacgagaTTTCCAAGACGCTGGGAAGACATAACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                <  2:1501480/135‑1 (MQ=255)
                                                                                                       cTCAAGATATTTCTCAGACGCTGGCAACACAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                <  2:1970745/140‑1 (MQ=255)
                                                                                                       acaaaGAGATTTCGAACACGCTGGGAACAGAACACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                <  2:636491/137‑1 (MQ=255)
                                                                                                        acactagaTTTCAAAGACGGTGGCAACAAAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACt                               <  2:2363530/135‑1 (MQ=255)
                                                                                                            gacttttCCAAGACGCTGGCAAGATAAAACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtgg                           <  2:1512304/136‑1 (MQ=255)
                                                                                                               aTTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                        >  2:394852/1‑140 (MQ=255)
                                                                                                                 ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                      >  1:972116/1‑140 (MQ=255)
                                                                                                                 ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                      >  1:469384/1‑140 (MQ=255)
                                                                                                                 ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                      >  1:2369389/1‑140 (MQ=255)
                                                                                                                 ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                      >  1:122793/1‑140 (MQ=255)
                                                                                                                 ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                      >  1:1140266/1‑140 (MQ=255)
                                                                                                                 ttCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                      >  1:1597058/1‑140 (MQ=255)
                                                                                                                         ggCTGGGATGATATTACTCACGACTCCGGGAGCAATCGGAAACTCAGGGAGCTGAAGAACCATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGTTGATGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTc              <  2:567395/139‑1 (MQ=255)
                                                                                                                         agCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTc              <  2:2340252/140‑1 (MQ=255)
                                                                                                                         agCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTc              <  2:55749/140‑1 (MQ=255)
                                                                                                                         agCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTc              <  2:548589/140‑1 (MQ=255)
                                                                                                                          gCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTcc             >  2:1352259/1‑140 (MQ=255)
                                                                                                                                   aaaaaaCAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTGCATGTGAAACAGGTGAAGCCGAAAAAGCAGGCCTCACTTTGGTGGATGTATATTCCTGCTTTGct    <  1:1938074/135‑1 (MQ=255)
                                                                                                                                     aaaacacacGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct  <  1:62957/137‑1 (MQ=255)
                                                                                                                                     aaTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct  <  2:2428205/140‑1 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct  >  1:1575159/1‑140 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct  >  2:27396/1‑140 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct  <  2:732156/140‑1 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct  >  1:732156/1‑140 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct  <  2:1426708/140‑1 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACCTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAGGTATATTCCTGCTTTGctct  >  2:1247831/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                     
ATGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCT  >  NZ_CP034943/2901533‑2901804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: