Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,917,007	A→G	5.6%	D220D (GAT→GAC)	ytrF ←	ABC transporter permease YtrF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,917,007	0	A	G	5.6%	227.3 / 3.1	74	D220D (GAT→GAC)	ytrF	ABC transporter permease YtrF
Reads supporting (aligned to +/- strand):  ref base A (17/51);  new base G (2/2);  total (19/55)
Fisher's exact test for biased strand distribution p-value = 2.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

TTGTCAGTTGTTCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATA  >  NZ_CP034943/2916868‑2917138                                                                                                                                            |                                                                                                                                    ttGTCAGTTGTTCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCaa                                                                                                                                     >  1:1965488/1‑140 (MQ=255) ttGTCAGTTGTTCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGGCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCaa                                                                                                                                     >  1:949076/1‑140 (MQ=255)         tgttCCACGTTCTCAAATTTATCAGCAAAAACACCAATTTTTTTTTCAAGATTTCCGTCTTTGAAGTCTAAGAATTCCGACAAATGTTTCTTGAATTGATGACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTgaga                                                                                                                             <  1:1299471/140‑1 (MQ=255)         tgttCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTgaga                                                                                                                             <  1:382046/140‑1 (MQ=255)         tgttCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTgaga                                                                                                                             <  1:975869/140‑1 (MQ=255)         tgttCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTgaga                                                                                                                             <  1:185871/140‑1 (MQ=255)         tgttCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATGTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTgaga                                                                                                                             <  1:1964902/140‑1 (MQ=255)          gttCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGAt                                                                                                                            <  2:953436/140‑1 (MQ=255)          gttCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGAt                                                                                                                            <  2:586151/140‑1 (MQ=255)           ttCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCAAATCTTGAGATg                                                                                                                           >  1:1904582/1‑140 (MQ=255)           ttCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACAGTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAGTGTTTGAGTCCTCCATCCCATCTTGAGATg                                                                                                                           >  1:1549127/1‑140 (MQ=255)               aCGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCtt                                                                                                                       <  2:1073065/140‑1 (MQ=255)                  ttCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCttttt                                                                                                                    >  2:1567587/1‑140 (MQ=255)                  ttCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCttttt                                                                                                                    >  1:1502086/1‑140 (MQ=255)                  ttCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCtattt                                                                                                                    >  2:1160454/1‑140 (MQ=255)                  ttCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAAGCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCttttt                                                                                                                    >  2:868476/1‑140 (MQ=255)                   tctcAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCGTCCATCCAATCTTGAGATGGCtttttt                                                                                                                   >  2:1620553/1‑140 (MQ=255)                             atcatcAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaa                                                                                                         <  2:1658133/140‑1 (MQ=255)                             aTCAGCAAAAACACCAATGTTCGTTTCAACATTTCCGCCTTTGAAATCTAAGAATTCCGAAAAATCTTTTTTGAATTGATCACTGATAAAAATCTTTGAGTCCTCCATCCAGTCGTGAGATGGTTTTTTTGTAATAccga                                                                                                         >  2:2055831/1‑138 (MQ=255)                             aTCAGCAAAAACACCAATGTTCGTTTCAACATTTCCGCCTTTGAAATCTAAGAATTCCGAAAAATCTTTTTTGAATTGATCACTGATAAAAATATTTGAGTCCTCCATCCAGTCTTGAGATGGTTTTTTTGTAATAccga                                                                                                         >  2:116324/1‑138 (MQ=255)                              tCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaac                                                                                                        <  1:1114878/140‑1 (MQ=255)                              tCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaac                                                                                                        <  1:1672304/140‑1 (MQ=255)                              tCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaac                                                                                                        <  1:2030067/140‑1 (MQ=255)                              tCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaac                                                                                                        <  1:1724287/140‑1 (MQ=255)                               cAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaaca                                                                                                       >  2:1588525/1‑140 (MQ=255)                                     aaaCACCAATTTTTTTTTTAAGATTTCCGTCTTTTAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCAATTATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTa                                                                                                 <  2:1911824/140‑1 (MQ=255)                                     aaaCACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTa                                                                                                 <  1:1044630/140‑1 (MQ=255)                                          ccAATGTTTTTTTCAACATTTCCGAGTTTTAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCCCTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAAGACCAACAATTTTAAAGTc                                                                                            <  1:2234382/140‑1 (MQ=255)                                            aaTGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCat                                                                                          <  2:973758/140‑1 (MQ=255)                                                        aaCATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTTTAATACCAACAATTTTAAAGTCATAtgtttttgttt                                                                              >  2:1416961/1‑140 (MQ=255)                                                                  tatttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                    <  2:2330176/138‑1 (MQ=255)                                                                  tCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                    <  2:370718/140‑1 (MQ=255)                                                                  tCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                    >  2:1305380/1‑140 (MQ=255)                                                                  tCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                    >  2:1796465/1‑140 (MQ=255)                                                                  tCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                    >  2:16832/1‑140 (MQ=255)                                                                  tCTTTGAAGTCTAAGAATTACGAAAAATCTTTATTGAATAGATCACTGATAAAAATGTATGAGACATCCATCCAAACTTTAGATGGATTTTTTGTAAAACCAACAATTTTAAACTAATAGGTTTTTGATGTTTATACATc                                                                    >  2:1302755/1‑140 (MQ=255)                                                                  tCTTTGAACTCTAAGAATTCCGAAAAATCTTAATTGAATTGATCACTGATAAAAATCATATAGTCCAACATACAATCTTGAGATGGCTTTTTTTTAATACCAACAATTTAAAAGTCATATGATTTTGTTTTTTCGACATc                                                                    >  2:954073/1‑140 (MQ=255)                                                                    tttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  <  2:906509/140‑1 (MQ=255)                                                                    tttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  <  2:971870/140‑1 (MQ=255)                                                                    tttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  <  2:9936/140‑1 (MQ=255)                                                                    tttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  <  2:1502086/140‑1 (MQ=255)                                                                    tttGAAGTCTAAGAATTCCGAAAAATCTTTATTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  <  2:788006/140‑1 (MQ=255)                                                                    tttGAAGTATAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  <  2:878800/140‑1 (MQ=255)                                                                           tatAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGtttt                                                           <  2:1597002/138‑1 (MQ=255)                                                                                gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCACTCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                      <  1:1658669/140‑1 (MQ=255)                                                                                gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                      <  1:363435/140‑1 (MQ=255)                                                                                gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                      <  1:1588525/140‑1 (MQ=255)                                                                                gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                      <  1:266366/140‑1 (MQ=255)                                                                                gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                      <  2:217640/140‑1 (MQ=255)                                                                                gAATTCCGAAAAATCTTTCTTGAAGTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                      <  1:953539/140‑1 (MQ=255)                                                                                           aaTCTTTCTTTAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGAt                                           <  2:1210323/140‑1 (MQ=255)                                                                                           aaTCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGAt                                           <  2:823064/140‑1 (MQ=255)                                                                                           aaTCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATATTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTTTTTTTTCTAAATAACATGTTTTAGGATCTGTTTTTGAt                                           <  2:302520/140‑1 (MQ=255)                                                                                           aaTCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTACATACAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAATTCATATGTTTTTTTTTTTTCTAAATAAAATTTTTTAGGATCTGTTTTTGAt                                           <  2:1647913/140‑1 (MQ=255)                                                                                                ttcttGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACt                                      <  2:1432688/140‑1 (MQ=255)                                                                                                ttcttGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACt                                      <  2:386721/140‑1 (MQ=255)                                                                                                ttcttGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACt                                      <  2:59809/140‑1 (MQ=255)                                                                                                        ttGATCACTGATAAATATGTTTGAGTCCTCCATCAAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTAATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCaa                              <  2:1755031/140‑1 (MQ=255)                                                                                                        ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  <  1:290303/104‑1 (MQ=255)                                                                                                        ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  >  2:1986593/1‑104 (MQ=255)                                                                                                        ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  >  2:290303/1‑104 (MQ=255)                                                                                                        ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                  <  1:1986593/104‑1 (MQ=255)                                                                                                        ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCaa                              <  2:888361/140‑1 (MQ=255)                                                                                                        ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCaa                              <  2:1884309/140‑1 (MQ=255)                                                                                                        ttGATAACTGATAAAAATGTTTGAGTCCTCGATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCaa                              <  2:1968665/140‑1 (MQ=255)                                                                                                                          ttttGAGTCCTCCATCCATTCTTGAGATTGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAatat            <  2:2058395/139‑1 (MQ=255)                                                                                                                          ttttGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACTATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTTTTTTTGATACAATTAATTCAATTGTTTTGTTCAGAatat            <  2:493221/139‑1 (MQ=255)                                                                                                                          gTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGTCATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAatat            >  2:1438387/1‑140 (MQ=255)                                                                                                                          gTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAatat            <  1:1305380/140‑1 (MQ=255)                                                                                                                             tGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCtt         <  2:709856/140‑1 (MQ=255)                                                                                                                                  cctccATCCAGTCTTGAGATGGTTTTTTTGTAATACCGACAATTTTAAAGTCATATGTTTTTGTTTTTGTAACATCCCCTGTTTTAGAATCTGTCTTCGATACACTTAGTTCAATTGTTTTATTTAGAATATCTTTCGta    <  1:116324/140‑1 (MQ=255)                                                                                                                                  cctccATCCAATCGTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTGTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGta    <  1:1354719/140‑1 (MQ=255)                                                                                                                                  actccATCCAGTCTTGAGATTGTTTTTTTGTAATACCGACAATTTTAAAGTCATATGTTTTTGTTTTTGTAACATCCCCTGTTTTAGAATCTGTCTTCGATACACTTAGTTCAATTGTTTTATTTAGAATATCTTTCGta    <  1:2055831/139‑1 (MQ=255)                                                                                                                                  actcaATCAAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGta    <  2:1436945/139‑1 (MQ=255)                                                                                                                                    tccctccaATCTTGAGATGTCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGtata  <  2:1923855/140‑1 (MQ=255)                                                                                                                                    tccatccaATCTTGAGATGTCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGtata  <  2:1926794/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                    TTGTCAGTTGTTCCACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATA  >  NZ_CP034943/2916868‑2917138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑