Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,917,019 C→T 5.1% K216K (AAG→AAA)  ytrF ← ABC transporter permease YtrF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,917,0190CT 5.1% 225.5 / 2.3 78K216K (AAG→AAA) ytrFABC transporter permease YtrF
Reads supporting (aligned to +/- strand):  ref base C (21/52);  new base T (2/2);  total (24/54)
Fisher's exact test for biased strand distribution p-value = 5.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTGGTTCTT  >  NZ_CP034943/2916882‑2917152
                                                                                                                                         |                                                                                                                                     
aCGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCtt                                                                                                                                     <  2:1073065/140‑1 (MQ=255)
   ttCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCttttt                                                                                                                                  >  1:1502086/1‑140 (MQ=255)
   ttCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCttttt                                                                                                                                  >  2:1567587/1‑140 (MQ=255)
   ttCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCtattt                                                                                                                                  >  2:1160454/1‑140 (MQ=255)
   ttCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAAGCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCttttt                                                                                                                                  >  2:868476/1‑140 (MQ=255)
    tctcAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCGTCCATCCAATCTTGAGATGGCtttttt                                                                                                                                 >  2:1620553/1‑140 (MQ=255)
              atcatcAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaa                                                                                                                       <  2:1658133/140‑1 (MQ=255)
              aTCAGCAAAAACACCAATGTTCGTTTCAACATTTCCGCCTTTGAAATCTAAGAATTCCGAAAAATCTTTTTTGAATTGATCACTGATAAAAATCTTTGAGTCCTCCATCCAGTCGTGAGATGGTTTTTTTGTAATAccga                                                                                                                       >  2:2055831/1‑138 (MQ=255)
              aTCAGCAAAAACACCAATGTTCGTTTCAACATTTCCGCCTTTGAAATCTAAGAATTCCGAAAAATCTTTTTTGAATTGATCACTGATAAAAATATTTGAGTCCTCCATCCAGTCTTGAGATGGTTTTTTTGTAATAccga                                                                                                                       >  2:116324/1‑138 (MQ=255)
               tCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaac                                                                                                                      <  1:1114878/140‑1 (MQ=255)
               tCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaac                                                                                                                      <  1:1672304/140‑1 (MQ=255)
               tCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaac                                                                                                                      <  1:1724287/140‑1 (MQ=255)
               tCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaac                                                                                                                      <  1:2030067/140‑1 (MQ=255)
                cAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACcaaca                                                                                                                     >  2:1588525/1‑140 (MQ=255)
                      aaaCACCAATTTTTTTTTTAAGATTTCCGTCTTTTAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCAATTATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTa                                                                                                               <  2:1911824/140‑1 (MQ=255)
                      aaaCACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTa                                                                                                               <  1:1044630/140‑1 (MQ=255)
                           ccAATGTTTTTTTCAACATTTCCGAGTTTTAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCCCTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAAGACCAACAATTTTAAAGTc                                                                                                          <  1:2234382/140‑1 (MQ=255)
                             aaTGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCat                                                                                                        <  2:973758/140‑1 (MQ=255)
                                         aaCATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTTTAATACCAACAATTTTAAAGTCATAtgtttttgttt                                                                                            >  2:1416961/1‑140 (MQ=255)
                                                   tatttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                                  <  2:2330176/138‑1 (MQ=255)
                                                   tCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                                  >  2:1796465/1‑140 (MQ=255)
                                                   tCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                                  <  2:370718/140‑1 (MQ=255)
                                                   tCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                                  >  2:16832/1‑140 (MQ=255)
                                                   tCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATc                                                                                  >  2:1305380/1‑140 (MQ=255)
                                                   tCTTTGAAGTCTAAGAATTACGAAAAATCTTTATTGAATAGATCACTGATAAAAATGTATGAGACATCCATCCAAACTTTAGATGGATTTTTTGTAAAACCAACAATTTTAAACTAATAGGTTTTTGATGTTTATACATc                                                                                  >  2:1302755/1‑140 (MQ=255)
                                                   tCTTTGAACTCTAAGAATTCCGAAAAATCTTAATTGAATTGATCACTGATAAAAATCATATAGTCCAACATACAATCTTGAGATGGCTTTTTTTTAATACCAACAATTTAAAAGTCATATGATTTTGTTTTTTCGACATc                                                                                  >  2:954073/1‑140 (MQ=255)
                                                     tttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                <  2:9936/140‑1 (MQ=255)
                                                     tttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                <  2:971870/140‑1 (MQ=255)
                                                     tttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                <  2:906509/140‑1 (MQ=255)
                                                     tttGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                <  2:1502086/140‑1 (MQ=255)
                                                     tttGAAGTCTAAGAATTCCGAAAAATCTTTATTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                <  2:788006/140‑1 (MQ=255)
                                                     tttGAAGTATAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                <  2:878800/140‑1 (MQ=255)
                                                            tatAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGtttt                                                                         <  2:1597002/138‑1 (MQ=255)
                                                                 gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCACTCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                                    <  1:1658669/140‑1 (MQ=255)
                                                                 gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                                    <  2:217640/140‑1 (MQ=255)
                                                                 gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                                    <  1:363435/140‑1 (MQ=255)
                                                                 gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                                    <  1:266366/140‑1 (MQ=255)
                                                                 gAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                                    <  1:1588525/140‑1 (MQ=255)
                                                                 gAATTCCGAAAAATCTTTCTTGAAGTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGAt                                                                    <  1:953539/140‑1 (MQ=255)
                                                                            aaTCTTTCTTTAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGAt                                                         <  2:1210323/140‑1 (MQ=255)
                                                                            aaTCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGAt                                                         <  2:823064/140‑1 (MQ=255)
                                                                            aaTCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATATTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTTTTTTTTCTAAATAACATGTTTTAGGATCTGTTTTTGAt                                                         <  2:302520/140‑1 (MQ=255)
                                                                            aaTCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTACATACAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAATTCATATGTTTTTTTTTTTTCTAAATAAAATTTTTTAGGATCTGTTTTTGAt                                                         <  2:1647913/140‑1 (MQ=255)
                                                                                 ttcttGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACt                                                    <  2:59809/140‑1 (MQ=255)
                                                                                 ttcttGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACt                                                    <  2:386721/140‑1 (MQ=255)
                                                                                 ttcttGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACt                                                    <  2:1432688/140‑1 (MQ=255)
                                                                                         ttGATCACTGATAAATATGTTTGAGTCCTCCATCAAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTAATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCaa                                            <  2:1755031/140‑1 (MQ=255)
                                                                                         ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                >  2:290303/1‑104 (MQ=255)
                                                                                         ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                <  1:290303/104‑1 (MQ=255)
                                                                                         ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                <  1:1986593/104‑1 (MQ=255)
                                                                                         ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATccc                                                                                >  2:1986593/1‑104 (MQ=255)
                                                                                         ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCaa                                            <  2:888361/140‑1 (MQ=255)
                                                                                         ttGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCaa                                            <  2:1884309/140‑1 (MQ=255)
                                                                                         ttGATAACTGATAAAAATGTTTGAGTCCTCGATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCaa                                            <  2:1968665/140‑1 (MQ=255)
                                                                                                           ttttGAGTCCTCCATCCATTCTTGAGATTGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAatat                          <  2:2058395/139‑1 (MQ=255)
                                                                                                           ttttGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACTATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTTTTTTTGATACAATTAATTCAATTGTTTTGTTCAGAatat                          <  2:493221/139‑1 (MQ=255)
                                                                                                           gTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGTCATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAatat                          >  2:1438387/1‑140 (MQ=255)
                                                                                                           gTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAatat                          <  1:1305380/140‑1 (MQ=255)
                                                                                                              tGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCtt                       <  2:709856/140‑1 (MQ=255)
                                                                                                                   cctccATCCAGTCTTGAGATGGTTTTTTTGTAATACCGACAATTTTAAAGTCATATGTTTTTGTTTTTGTAACATCCCCTGTTTTAGAATCTGTCTTCGATACACTTAGTTCAATTGTTTTATTTAGAATATCTTTCGta                  <  1:116324/140‑1 (MQ=255)
                                                                                                                   cctccATCCAATCGTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTGTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGta                  <  1:1354719/140‑1 (MQ=255)
                                                                                                                   actccATCCAGTCTTGAGATTGTTTTTTTGTAATACCGACAATTTTAAAGTCATATGTTTTTGTTTTTGTAACATCCCCTGTTTTAGAATCTGTCTTCGATACACTTAGTTCAATTGTTTTATTTAGAATATCTTTCGta                  <  1:2055831/139‑1 (MQ=255)
                                                                                                                   actcaATCAAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGta                  <  2:1436945/139‑1 (MQ=255)
                                                                                                                     tccctccaATCTTGAGATGTCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGtata                <  2:1923855/140‑1 (MQ=255)
                                                                                                                     tccatccaATCTTGAGATGTCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGtata                <  2:1926794/140‑1 (MQ=255)
                                                                                                                              tCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTgg       <  1:355714/140‑1 (MQ=255)
                                                                                                                              tCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTgg       <  1:1596677/140‑1 (MQ=255)
                                                                                                                              tCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTgg       <  1:2318209/140‑1 (MQ=255)
                                                                                                                              tCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTgg       <  1:719707/140‑1 (MQ=255)
                                                                                                                              tCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTgg       <  1:627878/140‑1 (MQ=255)
                                                                                                                              tCTTGAGATGGCTTTTTTGTAATACCAACAATTGTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGCTCTGGTGTTGCTACACTTAATTCAAGTGTTTTGTTCAGACTATCTTTCGTCTAGCGTTCTgg       >  2:40335/1‑140 (MQ=255)
                                                                                                                              tCTTGAGATGGCTTTTTTGTAATACAAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTgg       <  1:728250/140‑1 (MQ=255)
                                                                                                                               cTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTGGTATAGCCTGCTGGt      >  2:1888016/1‑140 (MQ=255)
                                                                                                                               cTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTGGt      >  2:751726/1‑140 (MQ=255)
                                                                                                                               cTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTGGt      >  2:1288642/1‑140 (MQ=255)
                                                                                                                               cTTGAGATGGCTGTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTGGt      >  2:1376111/1‑140 (MQ=255)
                                                                                                                                   agaTGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTTTTCAGAATATCTTTCGTATAGCCTTCTGGTTCtt  >  2:2010842/1‑140 (MQ=255)
                                                                                                                                   agaTGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTGGTTCtt  >  1:1544114/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                     
ACGTTCTCAAATTTATCAGCAAAAACACCAATGTTTTTTTCAACATTTCCGTCTTTGAAGTCTAAGAATTCCGAAAAATCTTTCTTGAATTGATCACTGATAAAAATGTTTGAGTCCTCCATCCAATCTTGAGATGGCTTTTTTGTAATACCAACAATTTTAAAGTCATATGTTTTTGTTTTTTCGACATCCCCTGTTTTAGGATCTGTTTTTGATACACTTAATTCAATTGTTTTGTTCAGAATATCTTTCGTATAGCCTTCTGGTTCTT  >  NZ_CP034943/2916882‑2917152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑