Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,928,089 T→A 5.2% intergenic (‑49/+91) alaP ← / ← asnB alanine permease AlaP/asparagine synthase (glutamine‑hydrolyzing)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,928,0890TA 5.2% 550.3 / 14.9 165intergenic (‑49/+91)alaP/asnBalanine permease AlaP/asparagine synthase (glutamine‑hydrolyzing)
Reads supporting (aligned to +/- strand):  ref base T (88/68);  new base A (5/4);  total (93/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 166 total aligned reads displayed.

CCACTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCTTCAGGCAT  >  NZ_CP034943/2927956‑2928227
                                                                                                                                     |                                                                                                                                          
ccACTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCgt                                                                                                                                      <  2:509026/140‑1 (MQ=255)
   cTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGtgtg                                                                                                                                   >  2:1747902/1‑140 (MQ=255)
   cTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGtgtg                                                                                                                                   >  1:1068408/1‑140 (MQ=255)
   cTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGtgtg                                                                                                                                   >  2:2433960/1‑140 (MQ=255)
     ccTATAGCAGCTCCTCAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTCGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTGTCCGTGTGGa                                                                                                                                 <  2:786048/140‑1 (MQ=255)
     ccTATAGCAGCTCCTAAAGACCTTACAGAAAGAGGCCATTCTTCGAGTCCGCGGTGCAGCACTTGTTTTTGTTTTTGCATGCGTTCTTCTCCCCTAGAAAAAAATCTCAATAAATCATGTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  1:1490594/140‑1 (MQ=255)
     ccTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  1:98438/140‑1 (MQ=255)
     ccTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  1:477788/140‑1 (MQ=255)
     ccTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  2:2140525/140‑1 (MQ=255)
     ccTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  1:176344/140‑1 (MQ=255)
       tataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTCTTCTCCCCTAGAAAAAAATATCATTATATCATTTTTCTGAATATTCCGTACGGATTg                                                                                                                              >  1:940722/1‑140 (MQ=255)
        ataGCAGCTCCTAAAGACATTAAAGAAATATGCGTTTCTTCGAGTCCGCGGTGCAGGTCTGGTTTTTGTGTTTGCATGCTTTCTTATACCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTGTCCGTGTGGATTg                                                                                                                              >  1:808345/1‑140 (MQ=255)
        ataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTg                                                                                                                              >  2:167714/1‑140 (MQ=255)
        ataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTg                                                                                                                              >  2:1169335/1‑140 (MQ=255)
        ataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTg                                                                                                                              >  1:101691/1‑140 (MQ=255)
         taGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTgg                                                                                                                             >  1:1764443/1‑140 (MQ=255)
         taGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTgg                                                                                                                             >  1:1378758/1‑140 (MQ=255)
          agcagcTCCTAAAGACATTATAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTATTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGACTTTTCCGTGTGGATTggg                                                                                                                            <  2:456653/140‑1 (MQ=255)
          agcagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTggg                                                                                                                            <  2:457194/140‑1 (MQ=255)
            cagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTa                                                                                                                          >  1:1083797/1‑140 (MQ=255)
              gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTGTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTGTCCGTGTGGATTGGGTaaa                                                                                                                        >  2:587442/1‑140 (MQ=255)
              gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCTTGTGGATTGGGTaaa                                                                                                                        >  1:2148524/1‑140 (MQ=255)
              gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTaaa                                                                                                                        >  2:25378/1‑140 (MQ=255)
              gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTaaa                                                                                                                        >  1:2306470/1‑140 (MQ=255)
              gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTaaa                                                                                                                        >  2:916461/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:1355252/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:2021322/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:2050378/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:139528/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:611226/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  1:693025/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  1:1598464/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGAGTGGGTAAAta                                                                                                                      >  2:1878223/1‑139 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGAGTGGGTAAATc                                                                                                                      >  2:2116855/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTATGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:338055/1‑140 (MQ=255)
                tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCGTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTCAATTTTCCGAGTGGATTGGGTAAATc                                                                                                                      >  2:1659864/1‑140 (MQ=255)
                 ccTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCAGTGTGGATTGGGTAAATCa                                                                                                                     >  2:1339446/1‑140 (MQ=255)
                  cTAAAGACATTAAAGACATATCCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTGGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCaa                                                                                                                    <  2:67323/140‑1 (MQ=255)
                  cTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCAGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCaa                                                                                                                    <  2:867937/140‑1 (MQ=255)
                         cATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAatgatgat                                                                                                             >  2:2224012/1‑140 (MQ=255)
                         cATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTCTTCTCCCCTAGAAAAAAATATCATTATATCATTTTTCTGAATATTCCGTACGGATTGGGTCAATCAatgatgatg                                                                                                            >  2:1477529/1‑140 (MQ=255)
                            tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAAtgatgatgat                                                                                                          >  2:1208836/1‑139 (MQ=255)
                            tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  1:2095183/1‑140 (MQ=255)
                            tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  2:466044/1‑140 (MQ=255)
                            tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  2:152210/1‑140 (MQ=255)
                            tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  2:1287978/1‑140 (MQ=255)
                            tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  2:55750/1‑140 (MQ=255)
                              aaGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaaga                                                                                                        <  2:500989/140‑1 (MQ=255)
                                    tatGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAaataat                                                                                                  >  2:2378036/1‑140 (MQ=255)
                                     atGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAataata                                                                                                 <  1:60836/140‑1 (MQ=255)
                                       gCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATaaa                                                                                               <  2:360694/140‑1 (MQ=255)
                                       gCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATaaa                                                                                               <  2:1629330/140‑1 (MQ=255)
                                            tcttctAGTCTGCTGTGGAGATATTTTTTTTGTTTTTGAATTCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTTTGTAAATCAATGATGATTAATAAATAATAAAAGGat                                                                                          <  2:2001864/140‑1 (MQ=255)
                                            tctgcGATTACGCGGTGCAGCTATTTATTGTGTTATTGCATGCTTTCTTCTCCCCCAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTTGATTGGGTAAATCAATGATTATGAAGAAATAATAAACGGat                                                                                          <  2:888459/140‑1 (MQ=255)
                                              ttcGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGataa                                                                                        >  2:191621/1‑140 (MQ=255)
                                                      cgcgGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATaaa                                                                                               >  1:369279/1‑125 (MQ=255)
                                                      cgcgGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATaaa                                                                                               <  2:369279/125‑1 (MQ=255)
                                                      cgcgGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATaaa                                                                                               >  1:83103/1‑125 (MQ=255)
                                                             cAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTcga                                                                         >  1:2221620/1‑140 (MQ=255)
                                                             cAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTcga                                                                         >  1:2221489/1‑140 (MQ=255)
                                                             cAGCTCGTGTTTTTGTGTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATAGCAATAAATCATATTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGTATAATAACAAGTCTTTaga                                                                         >  1:693470/1‑140 (MQ=255)
                                                              aGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTcgac                                                                        <  2:853976/140‑1 (MQ=255)
                                                                  ctTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATAAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAAAAAGGCTTTCGACGAAt                                                                    >  2:2190542/1‑140 (MQ=255)
                                                                     gtttttgtttttGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGa                                                                 >  1:2248958/1‑140 (MQ=255)
                                                                     gtttttgtttttGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGa                                                                 >  1:2068353/1‑140 (MQ=255)
                                                                     gttcttgattgtGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGa                                                                 <  2:2367659/140‑1 (MQ=255)
                                                                               ttgCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCttgtt                                                       <  1:1015708/140‑1 (MQ=255)
                                                                               ttgCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCttgtt                                                       <  2:693048/140‑1 (MQ=255)
                                                                                    tGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTg                                                  <  2:661660/140‑1 (MQ=255)
                                                                                     gCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGt                                                 <  1:691691/140‑1 (MQ=255)
                                                                                     gCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGt                                                 <  1:1860209/140‑1 (MQ=255)
                                                                                         tcttctCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGACTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTaca                                             >  2:1241885/1‑140 (MQ=255)
                                                                                              tcCCCTATAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAg                                        <  1:2050516/140‑1 (MQ=255)
                                                                                              tcCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAg                                        <  1:1404245/140‑1 (MQ=255)
                                                                                                cccTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGAt                                      >  2:419721/1‑140 (MQ=255)
                                                                                                cccTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGAt                                      >  2:2407386/1‑140 (MQ=255)
                                                                                                cccTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGAGTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTGGTTGTCTGTTACACAAAGAt                                      >  1:1950649/1‑140 (MQ=255)
                                                                                                  cTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTTTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGa                                    <  2:1988403/140‑1 (MQ=255)
                                                                                                  cTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGa                                    <  1:1331996/140‑1 (MQ=255)
                                                                                                  cTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGa                                    <  1:2165745/140‑1 (MQ=255)
                                                                                                   tAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGACGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGAc                                   >  2:2309103/1‑140 (MQ=255)
                                                                                                   tAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGAGTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGAc                                   >  2:674043/1‑140 (MQ=255)
                                                                                                   tAGAAAAAAATATCAATAAACCATTTTTCTGAATTTTCCGTGTGGAGTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGAc                                   >  1:356075/1‑140 (MQ=255)
                                                                                                         aaaaTATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCttt                             <  2:830398/140‑1 (MQ=255)
                                                                                                         aaaaTATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCttt                             <  2:1086078/140‑1 (MQ=255)
                                                                                                                cactcaaTCATTTTTCTGAATTTTCCCTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCtga                      <  1:587442/135‑1 (MQ=255)
                                                                                                                cAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCtga                      <  1:916461/140‑1 (MQ=255)
                                                                                                                cAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCtga                      <  1:55955/140‑1 (MQ=255)
                                                                                                                cAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATAAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGAAGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCtga                      <  1:55750/140‑1 (MQ=255)
                                                                                                                       tCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCa               <  1:675223/140‑1 (MQ=255)
                                                                                                                        cATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAg              <  1:1642067/140‑1 (MQ=255)
                                                                                                                        cATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAg              <  2:1598464/140‑1 (MQ=255)
                                                                                                                         aTTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGc             >  2:2269261/1‑140 (MQ=255)
                                                                                                                          tttttCTGAATATTCCGTACGGATTGGGTAAATCAATGATGATGGAGA‑‑‑AATAAAAGGATAACAACAAGGCTTTCGACTACTCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTAGGCTGATGGCTCAGCTCtt         <  2:940722/140‑1 (MQ=255)
                                                                                                                          tttttCTGAATATTCCGTACGGATTGGGTAAATCAATGATGATGGAGA‑‑‑AATAAAAGGATAACAACAAGGCTTTCGACTACTCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTAGGCTGATGGCTCAGCTCtt         <  2:681411/140‑1 (MQ=255)
                                                                                                                          tttttCTGAATATTCCGTACGGATTGGGTAAATCAATGATGATGGAGA‑‑‑AATAAAAGGATAACAACAAGGCTTTCGACTACTCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTAGGCTGATGGCTCAGCTCtt         <  1:625544/140‑1 (MQ=255)
                                                                                                                          tttttCTGAATATTCCGTACGGATTGGGTAAATCAATGATGATGGAGA‑‑‑AATAAAAGGATAACAACAAGGCTTTCGACTACTCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTAGGCTGATGGCTCAGCTCtt         <  1:2331193/140‑1 (MQ=255)
                                                                                                                             ttCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCtt         <  1:2433960/140‑1 (MQ=255)
                                                                                                                             ttCTGAATTTTCCGTGTGGACTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCtt         <  1:1241885/140‑1 (MQ=255)
                                                                                                                                    ttttCCGTGTGGATTGGGTAAATCACTGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCTTCAGGCat  >  2:920194/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                          
CCACTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCTTCAGGCAT  >  NZ_CP034943/2927956‑2928227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: