Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,928,097 T→C 5.6% intergenic (‑57/+83) alaP ← / ← asnB alanine permease AlaP/asparagine synthase (glutamine‑hydrolyzing)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,928,0970TC 5.6% 513.0 / 13.6 159intergenic (‑57/+83)alaP/asnBalanine permease AlaP/asparagine synthase (glutamine‑hydrolyzing)
Reads supporting (aligned to +/- strand):  ref base T (84/66);  new base C (5/4);  total (89/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 164 total aligned reads displayed.

CTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCTTCAGGCAT  >  NZ_CP034943/2927959‑2928227
                                                                                                                                          |                                                                                                                                  
cTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGtgtg                                                                                                                                   >  1:1068408/1‑140 (MQ=255)
cTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGtgtg                                                                                                                                   >  2:2107105/1‑140 (MQ=255)
cTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGtgtg                                                                                                                                   >  2:1747902/1‑140 (MQ=255)
cTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGtgtg                                                                                                                                   >  2:524023/1‑140 (MQ=255)
  ccTATAGCAGCTCCTCAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTCGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTGTCCGTGTGGa                                                                                                                                 <  2:786048/140‑1 (MQ=255)
  ccTATAGCAGCTCCTAAAGACCTTACAGAAAGAGGCCATTCTTCGAGTCCGCGGTGCAGCACTTGTTTTTGTTTTTGCATGCGTTCTTCTCCCCTAGAAAAAAATCTCAATAAATCATGTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  1:1490594/140‑1 (MQ=255)
  ccTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  1:176344/140‑1 (MQ=255)
  ccTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  2:2140525/140‑1 (MQ=255)
  ccTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  1:707070/140‑1 (MQ=255)
  ccTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTAGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGa                                                                                                                                 <  1:588027/140‑1 (MQ=255)
    tataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTCTTCTCCCCTAGAAAAAAATATCATTATATCATTTTTCTGAATATTCCGTACGGATTg                                                                                                                              >  1:940722/1‑140 (MQ=255)
    tataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGCCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTCTTCTCCCCTAGAAAAAAATATCATTATATCATTTTTCTGAATATTCCGTACGGATTg                                                                                                                              >  1:681411/1‑140 (MQ=255)
     ataGCAGCTCCTAAAGACATTAAAGAAATATGCGTTTCTTCGAGTCCGCGGTGCAGGTCTGGTTTTTGTGTTTGCATGCTTTCTTATACCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTGTCCGTGTGGATTg                                                                                                                              >  1:808345/1‑140 (MQ=255)
     ataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTg                                                                                                                              >  2:1169335/1‑140 (MQ=255)
     ataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTg                                                                                                                              >  2:167714/1‑140 (MQ=255)
     ataGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTg                                                                                                                              >  1:101691/1‑140 (MQ=255)
      taGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTgg                                                                                                                             >  1:1764443/1‑140 (MQ=255)
      taGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTgg                                                                                                                             >  1:1378758/1‑140 (MQ=255)
       agcagcTCCTACAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCATGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTggg                                                                                                                            <  2:1507755/140‑1 (MQ=255)
       agcagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTggg                                                                                                                            <  2:2205112/140‑1 (MQ=255)
       agcagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTggg                                                                                                                            <  2:1226858/140‑1 (MQ=255)
       agcagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTggg                                                                                                                            <  2:1346149/140‑1 (MQ=255)
       agcagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTggg                                                                                                                            <  2:35260/140‑1 (MQ=255)
       agcagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTggg                                                                                                                            <  2:435854/140‑1 (MQ=255)
       agcagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTggg                                                                                                                            <  2:457194/140‑1 (MQ=255)
         cagcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTa                                                                                                                          >  1:1083797/1‑140 (MQ=255)
           gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTGTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTGTCCGTGTGGATTGGGTaaa                                                                                                                        >  2:587442/1‑140 (MQ=255)
           gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCTTGTGGATTGGGTaaa                                                                                                                        >  1:2148524/1‑140 (MQ=255)
           gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTaaa                                                                                                                        >  2:25378/1‑140 (MQ=255)
           gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTaaa                                                                                                                        >  2:916461/1‑140 (MQ=255)
           gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTaaa                                                                                                                        >  2:1072802/1‑140 (MQ=255)
           gcTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTaaa                                                                                                                        >  1:853976/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:139528/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  1:1598464/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:1096787/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  1:693048/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  1:693025/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:459395/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:2021322/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  1:500989/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:2050378/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  1:2174036/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGAGTGGGTAAAta                                                                                                                      >  2:1878223/1‑139 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTATGAATTTTCCGTGTGGATTGGGTAAATc                                                                                                                      >  2:338055/1‑140 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTGTGGTTGGTGCATGCTTTCTTCTCCCCTAGAAAAATATATCAATAATTCATTTTTCTGCATTTTCCGTGTGGATTGGGTACAta                                                                                                                      >  2:1259161/1‑139 (MQ=255)
             tCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCGTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTCAATTTTCCGAGTGGATTGGGTAAATc                                                                                                                      >  2:1659864/1‑140 (MQ=255)
               cTAAAGACATTAAAGACATATCCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTGGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCaa                                                                                                                    <  2:67323/140‑1 (MQ=255)
               cTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCAGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCaa                                                                                                                    <  2:867937/140‑1 (MQ=255)
                      cATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTCTTCTCCCCTAGAAAAAAATATCATTATATCATTTTTCTGAATATTCCGTACGGATTGGGTAAATCAatgatgatg                                                                                                            >  2:625544/1‑140 (MQ=255)
                      cATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTCTTCTCCCCTAGAAAAAAATATCATTATATCATTTTTCTGAATATTCCGTACGGATTGGGTAAATCAatgatgatg                                                                                                            >  2:2331193/1‑140 (MQ=255)
                       aTTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAatgatgatg                                                                                                            >  2:1389800/1‑140 (MQ=255)
                       aTTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAatgatgatg                                                                                                            >  1:661660/1‑140 (MQ=255)
                       aTTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAatgatgatg                                                                                                            >  1:569823/1‑140 (MQ=255)
                         tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  2:1287978/1‑140 (MQ=255)
                         tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  2:152210/1‑140 (MQ=255)
                         tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  2:2426180/1‑140 (MQ=255)
                         tAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATgaa                                                                                                          >  2:55750/1‑140 (MQ=255)
                                 tatGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAaataat                                                                                                  >  2:2378036/1‑140 (MQ=255)
                                 tatGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAaataat                                                                                                  >  2:398755/1‑140 (MQ=255)
                                    gCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATaaa                                                                                               <  2:1629330/140‑1 (MQ=255)
                                         tcttctAGTCTGCTGTGGAGATATTTTTTTTGTTTTTGAATTCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTTTGTAAATCAATGATGATTAATAAATAATAAAAGGat                                                                                          <  2:2001864/140‑1 (MQ=255)
                                         tctgcGATTACGCGGTGCAGCTATTTATTGTGTTATTGCATGCTTTCTTCTCCCCCAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTTGATTGGGTAAATCAATGATTATGAAGAAATAATAAACGGat                                                                                          <  2:888459/140‑1 (MQ=255)
                                           ttcGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGataa                                                                                        >  2:191621/1‑140 (MQ=255)
                                                gTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACa                                                                                   >  1:935162/1‑140 (MQ=255)
                                                   cgcgGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATaaa                                                                                               >  1:369279/1‑125 (MQ=255)
                                                   cgcgGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATaaa                                                                                               <  2:369279/125‑1 (MQ=255)
                                                          cAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTcga                                                                         >  1:245657/1‑140 (MQ=255)
                                                          cAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTcga                                                                         >  1:2221620/1‑140 (MQ=255)
                                                           aGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTcgac                                                                        <  2:853976/140‑1 (MQ=255)
                                                               ctTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATAAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAAAAAGGCTTTCGACGAAt                                                                    >  2:2190542/1‑140 (MQ=255)
                                                                  gtttttgtttttGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGa                                                                 >  1:2068353/1‑140 (MQ=255)
                                                                  gtttttgtttttGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGa                                                                 >  1:2248958/1‑140 (MQ=255)
                                                                            ttgCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCttgtt                                                       <  2:693048/140‑1 (MQ=255)
                                                                            ttgCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCttgtt                                                       <  1:1015708/140‑1 (MQ=255)
                                                                            ttgCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCttgtt                                                       <  1:418572/140‑1 (MQ=255)
                                                                                 tGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTg                                                  <  2:661660/140‑1 (MQ=255)
                                                                                  gCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGt                                                 <  1:1860209/140‑1 (MQ=255)
                                                                                           tcCCCTATAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAg                                        <  1:2050516/140‑1 (MQ=255)
                                                                                           tcCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAg                                        <  1:1404245/140‑1 (MQ=255)
                                                                                             cccTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGAt                                      >  2:500283/1‑140 (MQ=255)
                                                                                             cccTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGAt                                      >  2:2407386/1‑140 (MQ=255)
                                                                                               cTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTTTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGa                                    <  2:1988403/140‑1 (MQ=255)
                                                                                               cTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGa                                    <  1:2165745/140‑1 (MQ=255)
                                                                                               cTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGa                                    <  1:1331996/140‑1 (MQ=255)
                                                                                                tAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGACGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGAc                                   >  2:2309103/1‑140 (MQ=255)
                                                                                                tAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGAc                                   >  1:209489/1‑140 (MQ=255)
                                                                                                tAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGAGTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGAc                                   >  2:674043/1‑140 (MQ=255)
                                                                                                      aaaaTATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCttt                             <  2:830398/140‑1 (MQ=255)
                                                                                                             cAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCtga                      <  1:55955/140‑1 (MQ=255)
                                                                                                                     cATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAg              <  1:1642067/140‑1 (MQ=255)
                                                                                                                      aTTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGc             >  2:2269261/1‑140 (MQ=255)
                                                                                                                       tttttCTGAATATTCCGTACGGATTGGGTAAATCAATGATGATGGAGA‑‑‑AATAAAAGGATAACAACAAGGCTTTCGACTACTCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTAGGCTGATGGCTCAGCTCtt         <  2:940722/140‑1 (MQ=255)
                                                                                                                       tttttCTGAATATTCCGTACGGATTGGGTAAATCAATGATGATGGAGA‑‑‑AATAAAAGGATAACAACAAGGCTTTCGACTACTCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTAGGCTGATGGCTCAGCTCtt         <  1:625544/140‑1 (MQ=255)
                                                                                                                       tttttCTGAATATTCCGTACGGATTGGGTAAATCAATGATGATGGAGA‑‑‑AATAAAAGGATAACAACAAGGCTTTCGACTACTCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTAGGCTGATGGCTCAGCTCtt         <  2:681411/140‑1 (MQ=255)
                                                                                                                       tttttCTGAATATTCCGTACGGATTGGGTAAATCAATGATGATGGAGA‑‑‑AATAAAAGGATAACAACAAGGCTTTCGACTACTCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTAGGCTGATGGCTCAGCTCtt         <  1:2331193/140‑1 (MQ=255)
                                                                                                                        ttttCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTGGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGctc           >  1:2253463/1‑140 (MQ=255)
                                                                                                                          ttCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCtt         <  1:191621/140‑1 (MQ=255)
                                                                                                                          ttCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCtt         <  1:2433960/140‑1 (MQ=255)
                                                                                                                          ttCTGAATTTTCCGTGTGGACTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCtt         <  1:1241885/140‑1 (MQ=255)
                                                                                                                                 ttttCCGTGTGGATTGGGTAAATCACTGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCTTCAGGCat  >  2:920194/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                  
CTCCTATAGCAGCTCCTAAAGACATTAAAGAAATATGCCTTTCTTCGAGTCCGCGGTGCAGCTCTTGTTTTTGTTTTTGCATGCTTTCTTCTCCCCTAGAAAAAAATATCAATAAATCATTTTTCTGAATTTTCCGTGTGGATTGGGTAAATCAATGATGATGAAGAAATAATAAAAGGATAATAACAAGGCTTTCGACGAATCGAAAGCCTTGTTGTCTGTTACACAAAGATGACTTCTTTCGGCTGATGACTCAGCTCTTCAGGCAT  >  NZ_CP034943/2927959‑2928227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: