Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,945,599 G→T 5.3% intergenic (+26/‑28) EO946_RS15185 → / → EO946_RS15190 cytochrome d ubiquinol oxidase subunit II/DUF1540 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,945,5990GT 5.3% 410.9 / 7.5 132intergenic (+26/‑28)EO946_RS15185/EO946_RS15190cytochrome d ubiquinol oxidase subunit II/DUF1540 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (61/63);  new base T (3/4);  total (64/68)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 132 total aligned reads displayed.

GAGCGCTGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGTGAAACGGCTGAAA  >  NZ_CP034943/2945464‑2945738
                                                                                                                                       |                                                                                                                                           
  gcgcTGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAATCCAGaa                                                                                                                                       <  1:61438/140‑1 (MQ=255)
  gcgcTGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGaa                                                                                                                                       <  1:913959/140‑1 (MQ=255)
     cTGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCt                                                                                                                                    >  2:356684/1‑140 (MQ=255)
      tGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCtt                                                                                                                                   >  2:1311381/1‑140 (MQ=255)
      tGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCtt                                                                                                                                   >  2:35319/1‑140 (MQ=255)
      tGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCtt                                                                                                                                   >  2:1004755/1‑140 (MQ=255)
      tGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCtt                                                                                                                                   >  2:664076/1‑140 (MQ=255)
      tGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCtt                                                                                                                                   >  2:41497/1‑140 (MQ=255)
                gTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACATTTATAaggagg                                                                                                                         >  2:1150948/1‑140 (MQ=255)
                  atatCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAgggg                                                                                                                       >  2:385374/1‑140 (MQ=255)
                   tatCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGa                                                                                                                      <  2:2024907/140‑1 (MQ=255)
                   tatCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGa                                                                                                                      <  2:1168129/140‑1 (MQ=255)
                   tatCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGa                                                                                                                      <  2:2267712/140‑1 (MQ=255)
                   tatCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGa                                                                                                                      <  2:130622/140‑1 (MQ=255)
                   tatCGTTGCATTTATCATTCTCTTTCCAGGCGTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGa                                                                                                                      <  2:129820/140‑1 (MQ=255)
                   tatCGTTGCATTTATAATTCTCTGTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGa                                                                                                                      <  2:1268095/140‑1 (MQ=255)
                    atCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGTACAAGCCCGAGGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGAt                                                                                                                     >  1:1161254/1‑140 (MQ=255)
                           cATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGaa                                                                                                                                       <  2:2315884/115‑1 (MQ=255)
                           cATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGaa                                                                                                                                       >  1:2315884/1‑115 (MQ=255)
                                aTCATTCTATTTCCGGGCTTCTTTTTCTTCTGGAAAATGTTTATGCGAGATAAACGCTATATCCGCCAGGAGGAATAGAGGGCAAGCCAGCCGGCTATACTAATCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAg                                                                                                         >  2:2014731/1‑140 (MQ=255)
                                aTCATTCTATTTCCGGGCTTCTTTTTCTTCTGGAAAATGTTTATGCGAGATAAACGCTATATCCGCCAGGAGGAATAGAGGGCAAGCCAGCCGGCTATACTAATCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAg                                                                                                         >  2:807288/1‑140 (MQ=255)
                                aTCATTCTATTTCCGGGCTTCTTTTTCTTCTGGAAAATGTTTATGCGAGATAAACGCTATATCCGCCAGGAGGAATAGAGGGCAAGCCAGCCGGCTATACTAATCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAg                                                                                                         >  2:807437/1‑140 (MQ=255)
                                 tCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGa                                                                                                        <  1:1679022/140‑1 (MQ=255)
                                  cATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCGTTATAAGGAGGGGATGGCATGGAGCAGaa                                                                                                       >  2:1663653/1‑140 (MQ=255)
                                     tctcTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAAGAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAAt                                                                                                    >  1:1992123/1‑140 (MQ=255)
                                          ttCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTAt                                                                                               >  2:1466067/1‑140 (MQ=255)
                                           tCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAAt                                                                                                    >  2:1551293/1‑134 (MQ=255)
                                           tCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAAt                                                                                                    <  1:1551293/134‑1 (MQ=255)
                                             ccggCTTCTATTTTTTCGGGAAACTGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCg                                                                                            <  1:942419/138‑1 (MQ=255)
                                             cAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCg                                                                                            <  1:448393/140‑1 (MQ=255)
                                             cAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCg                                                                                            <  1:448569/140‑1 (MQ=255)
                                             cAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCg                                                                                            <  1:768055/140‑1 (MQ=255)
                                             cAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTGTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCg                                                                                            <  1:724215/140‑1 (MQ=255)
                                                  ttCTTTTTCTTCTGGAAAATGTTTATGCGAGATAAACGCTATATCCGCCAGGAGGAATAGAGGGCAAGCCAGCCGGCTATACTAATCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGTGAGGTc                                                                                       <  1:807437/140‑1 (MQ=255)
                                                  ttCTTTTTCTTCTGGAAAATGTTTATGCGAGATAAACGCTATATCCGCCAGGAGGAATAGAGGGCAAGCCAGCCGGCTATACTAATCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGTGAGGTc                                                                                       <  1:807288/140‑1 (MQ=255)
                                                  ttCTTTTTCTTCTGGAAAATGTTTATGCGAGATAAACGCTATATCCGCCAGGAGGAATAGAGGGCAAGCCAGCCGGCTATACTAATCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGTGAGGTc                                                                                       <  1:2014731/140‑1 (MQ=255)
                                                     tATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaac                                                                                    >  2:470078/1‑140 (MQ=255)
                                                       ttttttCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGACCCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaacaa                                                                                  <  2:1916531/140‑1 (MQ=255)
                                                       ttttttCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaacaa                                                                                  <  1:1095885/140‑1 (MQ=255)
                                                       ttttttCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaacaa                                                                                  <  1:1442219/140‑1 (MQ=255)
                                                       ttttttCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaacaa                                                                                  <  2:344609/140‑1 (MQ=255)
                                                            tCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTATATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGtt                                                                             >  2:2366292/1‑140 (MQ=255)
                                                                        ttATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCa                                                                 <  2:1156407/140‑1 (MQ=255)
                                                                             cGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGa                                                            <  1:559815/140‑1 (MQ=255)
                                                                             cGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGa                                                            <  1:246057/140‑1 (MQ=255)
                                                                             cGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGa                                                            <  1:2280969/140‑1 (MQ=255)
                                                                             cGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGa                                                            <  1:987368/140‑1 (MQ=255)
                                                                             cGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCAAGAACCTTTATAATGAGGGGATGTCTTGGAGCAGAAAATTCTATGCGTAGTCAACAACTGTTCTTATTGGGGCAAAGGa                                                            <  1:1263260/140‑1 (MQ=255)
                                                                                 aTAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCCGAACCGTTATAAGGAGGGGATGGCATGGAGCAGACAATTCTATGCGAAGTCAACACCTGTTCTTATTGGGGCACAGGAACCa                                                        >  1:2161058/1‑140 (MQ=255)
                                                                                         tataTCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCAcc                                                >  1:2353011/1‑140 (MQ=255)
                                                                                         tataTCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCAcc                                                >  2:377467/1‑140 (MQ=255)
                                                                                         tataTCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCAcc                                                >  2:377686/1‑140 (MQ=255)
                                                                                          atatCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCg                                               >  2:173167/1‑140 (MQ=255)
                                                                                           tatCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATg                                                    >  2:137126/1‑134 (MQ=255)
                                                                                           tatCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATg                                                    <  1:137126/134‑1 (MQ=255)
                                                                                              ccGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaac                                                                                    <  2:923510/99‑1 (MQ=255)
                                                                                              ccGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaac                                                                                    >  1:923510/1‑99 (MQ=255)
                                                                                              ccGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaac                                                                                    <  2:2356743/99‑1 (MQ=255)
                                                                                              ccGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTcaac                                                                                    >  1:2356743/1‑99 (MQ=255)
                                                                                               cGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGAt                                          <  2:1095961/140‑1 (MQ=255)
                                                                                               cGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGAt                                          <  2:1959585/140‑1 (MQ=255)
                                                                                               cGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACCAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGAt                                          <  2:311851/140‑1 (MQ=255)
                                                                                                 ccAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTtat                                                                                                                                >  1:1406831/1‑52 (MQ=255)
                                                                                                 ccAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTtat                                                                                                                                <  2:1406831/52‑1 (MQ=255)
                                                                                                 ccAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGa                                                                                                        >  1:1058715/1‑76 (MQ=255)
                                                                                                 ccAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGa                                                                                                        <  2:1058715/76‑1 (MQ=255)
                                                                                                   aaGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCg                                                                                            <  1:2125608/86‑1 (MQ=255)
                                                                                                   aaGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCg                                                                                            >  2:2125608/1‑86 (MQ=255)
                                                                                                     gagGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCAtt                                    >  2:2194936/1‑140 (MQ=255)
                                                                                                      agGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCAttt                                   <  1:2247109/140‑1 (MQ=255)
                                                                                                      agGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCAttt                                   <  1:490277/140‑1 (MQ=255)
                                                                                                             gagGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTAtgttgt                            <  1:2194936/140‑1 (MQ=255)
                                                                                                              agGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTc                           >  2:2336237/1‑140 (MQ=255)
                                                                                                              agGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTc                           <  2:1825966/140‑1 (MQ=255)
                                                                                                              agGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTc                           >  1:1758066/1‑140 (MQ=255)
                                                                                                                gACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGc                                        >  1:995083/1‑125 (MQ=255)
                                                                                                                gACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAg                         >  1:757737/1‑140 (MQ=255)
                                                                                                                gACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAg                         >  1:567470/1‑140 (MQ=255)
                                                                                                                gACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAg                         >  2:1051394/1‑140 (MQ=255)
                                                                                                                gACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAg                         >  1:1242430/1‑140 (MQ=255)
                                                                                                                gACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAg                         >  1:106305/1‑140 (MQ=255)
                                                                                                                  cAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGcc                       >  2:2259975/1‑140 (MQ=255)
                                                                                                                  cAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGcc                       >  2:2262994/1‑140 (MQ=255)
                                                                                                                  cAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGcc                       >  2:1383252/1‑140 (MQ=255)
                                                                                                                  aaaGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGACATTTATGTTGTCAGcc                       >  2:1256132/2‑140 (MQ=255)
                                                                                                                   aaGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCa                      >  1:504688/1‑140 (MQ=255)
                                                                                                                   aaGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCa                      >  1:1696821/1‑140 (MQ=255)
                                                                                                                     gCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCaga                    >  2:1583185/1‑138 (MQ=255)
                                                                                                                     gCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATa                    >  1:489550/1‑140 (MQ=255)
                                                                                                                     gCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATa                    >  2:235026/1‑140 (MQ=255)
                                                                                                                      cccGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATAc                   <  1:35319/140‑1 (MQ=255)
                                                                                                                      cccGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATAc                   <  1:1004755/140‑1 (MQ=255)
                                                                                                                          aCGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGt               >  1:2404114/1‑140 (MQ=255)
                                                                                                                          aCGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGt               >  1:1979904/1‑140 (MQ=255)
                                                                                                                                      aGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGTGAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGTGAAACGGCTGaa   >  2:1428745/1‑140 (MQ=255)
                                                                                                                                      aGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGTGAAACGGCTGaa   >  1:2176833/1‑140 (MQ=255)
                                                                                                                                      aGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGTGAAACGGCTGaa   >  1:824441/1‑140 (MQ=255)
                                                                                                                                      aGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGTGAAACGGCTGaa   >  2:1051451/1‑140 (MQ=255)
                                                                                                                                      aGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGTGAAACGGCTGaa   >  2:1724072/1‑140 (MQ=255)
                                                                                                                                      aGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTCGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGTGAAACGGCTGaa   >  1:2025696/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                           
GAGCGCTGTTTGCGACGTATATCGTTGCATTTATCATTCTCTTTCCAGGCTTCTATTTTTTCTGGAAAATGTTTATGCGTGATAAACGGTATATCCGCCAAGAGGAATAGAGGACAAGCCCGACGGCTATACTAAGCCAGAACCTTTATAAGGAGGGGATGGCATGGAGCAGAAAATTCTATGCGAAGTCAACAACTGTTCTTATTGGGGCAAAGGAAACAAATGCACCGCTGATGCCATTTATGTTGTCAGCCATACTGGTGAAACGGCTGAAA  >  NZ_CP034943/2945464‑2945738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: