Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,991,252 C→A 23.0% intergenic (+53/‑154) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,991,2520CA23.0% 124.0 / 12.1 77intergenic (+53/‑154)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand):  ref base C (13/40);  new base A (18/2);  total (35/42)
Fisher's exact test for biased strand distribution p-value = 4.27e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTT  >  NZ_CP034943/2991119‑2991390
                                                                                                                                       |                                                                                                                                            
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAATTAAAACaaa                                                                                                                                       >  2:1275285/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTCTTTTTTATGTAATTAATACaaa                                                                                                                                       >  2:644757/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACTAaaaa                                                                                                                                       >  2:1312218/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTACTACaaa                                                                                                                                       >  2:2153252/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATACaaa                                                                                                                                       >  2:2287860/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATGTAAATAAAACaaa                                                                                                                                       >  2:1607599/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTTAATAGTAaaaa                                                                                                                                       >  1:2188523/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTTATTAATAaaaa                                                                                                                                       >  1:1287194/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTTATTAATACaaa                                                                                                                                       >  2:2152656/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATGTAATTACTACaaa                                                                                                                                       >  2:2145269/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATTTAATTAATAaaaa                                                                                                                                       >  2:2152585/1‑140 (MQ=255)
tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGAGTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTGTGTTGTTTTTTATGTAATTAATACaaa                                                                                                                                       >  2:238877/1‑140 (MQ=255)
 aatttgtttGGCTTAACGGGCTTGCTGAATCGTTGCAGAGCGGGTAATTTTTTAAATTTATACCTATTGAAAAAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAAt                                                                                                                                      <  1:1451776/133‑1 (MQ=255)
 aCTCTGATTGGCTGAAACGGCTTGCTGAATAGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAGTACacag                                                                                                                                      >  2:2108719/1‑137 (MQ=255)
 aCTATGTTTGGCTTAAACGGATTGCTTAAGGGGTGCAGCGGGGGTAATTTTTTAAATTTATACCTATTGAAAAAAATTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAAt                                                                                                                                      <  1:1647422/140‑1 (MQ=255)
 aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTCAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAAataataacaat                                                                                                                                      >  2:2201634/1‑135 (MQ=255)
 aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTATTTAAATAGTACaaaa                                                                                                                                      >  2:2108596/1‑139 (MQ=255)
 aCTATGATTGGCTGAAACCGCTAGCTCAATCGGTGCATAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAATTAAAACAAAAAAGCCTGGGAAACCGGGCGTGTTTTTTGTTTTGTTTTATTTATTAATAAAAAt                                                                                                                                      >  1:1012783/1‑140 (MQ=255)
      gATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGaa                                                                                                                                 <  2:1806816/140‑1 (MQ=255)
       aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAATAAAAAATTGaaa                                                                                                                                >  2:742446/1‑140 (MQ=255)
       aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAAAACAAAATTTaaa                                                                                                                                >  2:680460/1‑140 (MQ=255)
          ggCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTCTTTTTTATGTAATTAATACAAATTTGAAAAAt                                                                                                                             >  2:515107/1‑140 (MQ=255)
             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAAATACTAAAAATTTTAAAAATaag                                                                                                                          >  2:1591058/1‑138 (MQ=255)
             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATGTTATTTATACAAAATTTTAAAATaag                                                                                                                          >  2:37037/1‑138 (MQ=255)
                                gttGCCGAGCGGGCAAATTTTTAATTTTATACCGATTGAAACAAAGTAAAAAAAAAACGCCTTGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTAc                                                                                                      <  1:998439/138‑1 (MQ=255)
                                ggTGCAGAGCGGGTAAATTTTTAAATTTATACCTATTCAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTAc                                                                                                      <  1:1710431/140‑1 (MQ=255)
                                ggTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTAc                                                                                                      <  1:373660/140‑1 (MQ=255)
                                ggTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTAc                                                                                                      <  1:1710739/140‑1 (MQ=255)
                                ggTGCAGACCGGGTAAATTTTAAAATTTATCCCGAATGAAACAAACTAAAACAAAAACGCCTGGGAAACCGGTCGTTTATTTGTTCTTTTTTATGTAATTACGACACATTTGAAAAATACGGGAAAAACATCCCATTTAc                                                                                                      <  1:997947/140‑1 (MQ=255)
                                  tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTGTTTTTTTTTTTATTAATAAAAAATTTAAAAAAAACGGAAAAACAACACAATTaaat                                                                                                    >  1:862182/1‑137 (MQ=255)
                                           gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGTGTGGTGTGTTTTTTTTATTTGATTACTAACAAATTTGTAAATAAGGGAAAAAAAACCCCAGTACTTCCaaaaaaa                                                                                           >  1:1175606/1‑137 (MQ=255)
                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACGACAAATTTTAAAAATACGGGAAAAACCTCCCCTGTACTTCCAAAATAtt                                                                                          >  2:1784380/1‑140 (MQ=255)
                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATTACTACAAATTTGAAAAATACGGGAAAAACATGCCATTTACTTCCAAAAAAtt                                                                                          >  2:1226088/1‑140 (MQ=255)
                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTAATACAAAATTTAAAAATAATGGAAAAACAAACCAATTAAGTACCAAATAtt                                                                                          >  2:1558906/1‑140 (MQ=255)
                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTTTTTAATTTATAAAAAATTGTAAAATAATGGGAAAACATACCCAATTATTGCCaaaaatt                                                                                           >  1:2164478/1‑137 (MQ=255)
                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGGTTTGTTGTTTTTTTTTTTATTTCTTCAAAATTTATAAATAATGGGAAAAAATCCCAGTTAGTTACCAAAAAtt                                                                                          >  1:1664695/1‑140 (MQ=255)
                                                  ttttAAATTTATACCTATTGAAACAAATTAAACCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAACACATCCCATTTACTTCCAAAACATTTGATgg                                                                                    <  2:368913/140‑1 (MQ=255)
                                                            ataCCTATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                          <  2:318150/140‑1 (MQ=255)
                                                            ataCCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                          <  2:2202459/140‑1 (MQ=255)
                                                                 taTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                     <  2:30400/139‑1 (MQ=255)
                                                                 gATTCAAACAAAGTAACACACAACCGCCTGGGAAACCGCGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                     <  2:2143125/140‑1 (MQ=255)
                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTTTTTTAATTAAACAAAATTTAAAAATAAGGGAAAAAAATAACAATTAATTAAAAAAAATTTTATTGTAATtttatttattta                                                                    >  1:502952/1‑136 (MQ=255)
                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTACTACAAAATTGAAAAATAAGGGGAAAAAAAACCCTTTTCTTACAAAATATTTGAGGGTGATTTTTTGtgtgta                                                                    >  2:1539006/1‑135 (MQ=255)
                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTAATTACTACAAATTTTTAAAATACGGGAAAAACATACCATTTAATTCCAAAATATTTGATTGTAATTTTATGTAGGtt                                                                    >  2:564519/1‑139 (MQ=255)
                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTAATTAAATACTAAAAATTTGAAAAAATCGGGAAAAAAAACCCAGTTACTTCCAGAATATATTTTTGTAATTTTAtttgagtt                                                                    >  2:1406768/1‑133 (MQ=255)
                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATTACTACAAATTTTAAAAATAAGGGAAAAAAAAACCAATTACTTAAAAAATATTTTTTGGTTATTTTATGTAGGtt                                                                    >  2:2138775/1‑139 (MQ=255)
                                                                      aaaCAATGTAACAAAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAACTATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  1:1994771/140‑1 (MQ=255)
                                                                      aaaCAAATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  2:1673908/140‑1 (MQ=255)
                                                                            aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATTAATAAAAAATTTTAAAATAAGGGAAAAAAAAACCAATTTATTACAAAATATTTTATTGTAAATTTATGTAGGTAAAATTTTTAt                                                          >  2:194676/1‑140 (MQ=255)
                                                                            aGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTAGTTTTTATGTTATTACTACAAATTTGTAAAAAAAGGGAAAAACAGACCATTTACTTACAAAATATTTTTTGGTAATTTTATTGAGTTCAAATTTGttt                                                          >  2:2335299/1‑138 (MQ=255)
                                                                                aaccaacaaCGCCGGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:2358930/137‑1 (MQ=255)
                                                                                aaacaaacACGCCTGGTAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:769520/140‑1 (MQ=255)
                                                                                aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:2305165/140‑1 (MQ=255)
                                                                                aaaaaaaaACCCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:660230/140‑1 (MQ=255)
                                                                                aaaCAAAATCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:1136740/140‑1 (MQ=255)
                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:964099/140‑1 (MQ=255)
                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:742446/140‑1 (MQ=255)
                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTTATTAATACAAATTTTTAAAATAAGGGAAAAACATCCCCTTTACTTCCAAAATATTTTATGGTAATTTTTTTTAGGTAAAATTTGTATTTgg                                                      >  2:1562539/1‑140 (MQ=255)
                                                                                aaaCAAAAACCCCTGGGAAACCGGGCGTTTTTTTGTACTTATTTATGTAATTAATACAAAATAGAAAAATACCGCAAAAACATCCCATTGAATTCCAAAATATTTGATGGTAATTTAATGTAGGTAAAATGTGTATTTgg                                                      <  1:116930/140‑1 (MQ=255)
                                                                                 aaCAAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:680460/140‑1 (MQ=255)
                                                                                 aaCAAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:2078210/140‑1 (MQ=255)
                                                                                     aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAACAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAATTGGCCGtt                                                 <  1:2153252/140‑1 (MQ=255)
                                                                                     aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:1275285/140‑1 (MQ=255)
                                                                                     aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:1666048/140‑1 (MQ=255)
                                                                                     aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:2152656/140‑1 (MQ=255)
                                                                                     aaaaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:2145269/140‑1 (MQ=255)
                                                                                      accaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACGACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:1312218/137‑1 (MQ=255)
                                                                                      aacccGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:1908211/136‑1 (MQ=255)
                                                                                      aacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:2378990/137‑1 (MQ=255)
                                                                                      aacaCGCCTGGGAAACCGGGCGATTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                                 <  1:2152585/137‑1 (MQ=255)
                                                                                          cGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCaga                                             <  1:2423833/140‑1 (MQ=255)
                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  2:558469/140‑1 (MQ=255)
                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:1558906/140‑1 (MQ=255)
                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  2:1494659/140‑1 (MQ=255)
                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  2:1755476/140‑1 (MQ=255)
                                                                                                          cGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                             <  2:692087/140‑1 (MQ=255)
                                                                                                            tttttttGTTCTTTTTTATGTAAGTAATACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTCTGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCa                           <  2:1012969/140‑1 (MQ=255)
                                                                                                                ttttGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  2:862182/140‑1 (MQ=255)
                                                                                                                                      actacAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAATTTTGTATGTGGCCGTTCCGAGGCGGTGAAAAGCGGGCAGGCGTGAGTATTTTtatacgtttgt  >  2:1952085/1‑133 (MQ=255)
                                                                                                                                       |                                                                                                                                            
TACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTT  >  NZ_CP034943/2991119‑2991390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: