BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,995,560	C→T	5.0%	R10K (AGA→AAA)	lytG ←	exo‑glucosaminidase LytG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,995,560	0	C	T	5.0%	392.4 / 6.7	119	R10K (AGA→AAA)	lytG	exo‑glucosaminidase LytG
Reads supporting (aligned to +/- strand): ref base C (83/30); new base T (3/3); total (86/33)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 119 total aligned reads displayed.

ACAGGCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAG  >  NZ_CP034943/2995423‑2995698

aCAGGCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTa                                                                                                                                          <  2:935914/140‑1 (MQ=255)

   ggCTGTCTATAAATACCTGCTGTTAATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGAGAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGt                                                                                                                                       <  2:1061107/140‑1 (MQ=255)

    ggtGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  1:374449/3‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  2:791836/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  1:2071296/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  1:2340747/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  1:356553/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  2:1724904/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  2:1596286/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  2:1101468/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGtt                                                                                                                                      >  1:919939/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTGTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGATATGAAGAGTCTACGtt                                                                                                                                      >  1:80761/1‑140 (MQ=255)

    gCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGGTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTATCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACttt                                                                                                                                      >  1:417480/1‑140 (MQ=255)

     cTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGttt                                                                                                                                     >  2:1074999/1‑140 (MQ=255)

     cTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGttt                                                                                                                                     >  2:331107/1‑140 (MQ=255)

     cTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGttt                                                                                                                                     >  2:786363/1‑140 (MQ=255)

              aaTACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGttt                                                                                                                            >  2:1041071/1‑140 (MQ=255)

              aaTACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGttt                                                                                                                            >  1:290859/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGGTAGCGGAATCGATACAATGAAAAATCGTGAAATGAAGAGTCTACGTTTTTTCAGTGTTTTa                                                                                                                        >  2:2063208/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTCGCGGAATCGATACACTGAAATATAGTGAAATGTAGAGTCTACGTTTTTTCCGTTTTTTa                                                                                                                        >  1:2199840/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGACTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:1515085/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTCCGTTTTTTCAGTTTTTTa                                                                                                                        >  1:2152635/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:780050/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:755541/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:731640/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:6234/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:556744/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:413647/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:1587005/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:1612013/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:1170894/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTAAGTTTTTTa                                                                                                                        >  1:1865869/1‑140 (MQ=255)

                  ccTGCTGTTCATCAATTTCTTAGGGTTCTTTGGATGTTTCTATCGGTGTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAATATTCTACGTTTTTTCAGTTTTTTa                                                                                                                        >  1:1170199/1‑140 (MQ=255)

                    tgctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCa                                                                                                                                >  1:213158/1‑130 (MQ=255)

                    tgctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCa                                                                                                                                <  2:213158/130‑1 (MQ=255)

                    tgctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCa                                                                                                                                <  2:2419946/130‑1 (MQ=255)

                    tgctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCa                                                                                                                                >  1:2419946/1‑130 (MQ=255)

                     gctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACgg                                                                                                                     <  2:1259497/140‑1 (MQ=255)

                     gctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACgg                                                                                                                     <  1:1281716/140‑1 (MQ=255)

                     gctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACgg                                                                                                                     <  1:1168499/140‑1 (MQ=255)

                      ctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACACACAGGGCTAGCGGAATCGATACAATGAAATATAGTGAAATGAAGAGTCTACGTGTTTTCAGTTTTTTACggg                                                                                                                    >  2:1167913/1‑140 (MQ=255)

                      ctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACggg                                                                                                                    >  2:438498/1‑140 (MQ=255)

                      ctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACggg                                                                                                                    <  2:2071296/140‑1 (MQ=255)

                      ctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACggg                                                                                                                    <  2:356553/140‑1 (MQ=255)

                      ctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACggg                                                                                                                    >  1:1039490/1‑140 (MQ=255)

                      ctgTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAAGAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACggg                                                                                                                    >  1:1782566/1‑140 (MQ=255)

                         ttCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGATGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCa                                                                                                                 >  2:361108/1‑140 (MQ=255)

                           catcaATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATg                                                                                                               >  1:244131/1‑140 (MQ=255)

                                           ttCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAg                                                                                               >  1:630119/1‑140 (MQ=255)

                                                       ttCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTAc                                                                                   <  1:1482523/140‑1 (MQ=255)

                                                       ttCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTAc                                                                                   <  1:999994/140‑1 (MQ=255)

                                                       ttCAATCGGTTTTGATAAAGTTGTCGCTAAAACAAACAGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTAc                                                                                   >  1:330587/1‑140 (MQ=255)

                                                       ttCAATCGGTTTTGATAAAGTTGTCGCTAAAACAAACAGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTAc                                                                                   >  1:333879/1‑140 (MQ=255)

                                                       ttCAATCGGTTTTGATAAAGTTGTCGCTAAAACAAACAGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTAc                                                                                   >  1:1312095/1‑140 (MQ=255)

                                                        tCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGACTCGATACAATCAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCGTCGAGTTAAAAGATTAct                                                                                  >  2:2401602/1‑139 (MQ=255)

                                                         cTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGa                                                                                 <  2:290859/140‑1 (MQ=255)

                                                               gTTTTGATTATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACTATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaa                                                                           <  1:1275361/140‑1 (MQ=255)

                                                               gTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaa                                                                           <  1:808080/140‑1 (MQ=255)

                                                                ttttTATAATGTTGTGTCTTAAACAAACAGGGCTAGCGTAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCATTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaaa                                                                          <  2:1509758/140‑1 (MQ=255)

                                                                 tttGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaaaa                                                                         <  1:1124818/140‑1 (MQ=255)

                                                                 tttGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaaaa                                                                         <  1:1131141/140‑1 (MQ=255)

                                                                 tttGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaaaa                                                                         <  1:1696311/140‑1 (MQ=255)

                                                                             gtgGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCttt                                                             <  1:935712/140‑1 (MQ=255)

                                                                              tgGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCtttt                                                            >  1:2145368/1‑140 (MQ=255)

                                                                              tgGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCtttt                                                            >  1:1915246/1‑140 (MQ=255)

                                                                                    aaacaaacAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAAtt                                                      >  2:1973312/1‑140 (MQ=255)

                                                                                     aacaaacaGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGACttt                                                     >  2:1431685/1‑140 (MQ=255)

                                                                                     aacaaacaGGGCTAGCGGAATCGATACAATCAAAAATAGTGAAATGAAGAGTCTACGTTTGTTCAGTTTTTTACGGGCCATGAAAACCTTCGTTCGAGTTAAAAGATTACGAATGTAAAAGAATCGACCTGTTAGTAttt                                                     >  2:1736433/1‑140 (MQ=255)

                                                                                          acaGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACtt                                                >  2:1553499/1‑140 (MQ=255)

                                                                                           caGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                               <  2:1612013/140‑1 (MQ=255)

                                                                                           caGGGCTAGAGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                               <  2:244131/140‑1 (MQ=255)

                                                                                           caGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                               <  2:1312095/140‑1 (MQ=255)

                                                                                           caGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                               <  2:333879/140‑1 (MQ=255)

                                                                                              ggCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCAc                                            >  2:392679/1‑140 (MQ=255)

                                                                                              ggCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACATTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCAc                                            >  2:2222523/1‑140 (MQ=255)

                                                                                                cTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACtt                                          >  2:1094582/1‑140 (MQ=255)

                                                                                                cTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACtt                                          >  2:89686/1‑140 (MQ=255)

                                                                                                        aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAATAATATACCTTTTAGAATTTTACTTGAACTTCTAATAtt                                  >  1:526480/1‑140 (MQ=255)

                                                                                                        aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAtt                                  >  2:2114794/1‑140 (MQ=255)

                                                                                                        aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAtt                                  >  2:1522180/1‑140 (MQ=255)

                                                                                                        aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAtt                                  >  1:2092966/1‑140 (MQ=255)

                                                                                                        aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAtt                                  >  2:1522724/1‑140 (MQ=255)

                                                                                                        aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAtt                                  >  1:1969656/1‑140 (MQ=255)

                                                                                                         tCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAttt                                 >  1:1832000/1‑140 (MQ=255)

                                                                                                         tCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAttt                                 >  1:1832007/1‑140 (MQ=255)

                                                                                                         tCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAttt                                 >  1:1868365/1‑140 (MQ=255)

                                                                                                             tACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCCGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAACAGAATCTCCCTTTTAGAATTTTACTTGCACTTCTACTATTTTTCa                             >  2:1993725/1‑140 (MQ=255)

                                                                                                             tACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCa                             <  1:1973312/140‑1 (MQ=255)

                                                                                                                aaTGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTAAAtt                          >  1:1275880/1‑140 (MQ=255)

                                                                                                                    aaaaaTAGTGAAATGAAGACTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCttt                      <  2:1976603/140‑1 (MQ=255)

                                                                                                                     aaaaTAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTAAATTCTTTa                     >  2:1762346/1‑140 (MQ=255)

                                                                                                                           gTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCGTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACa               <  2:2000162/140‑1 (MQ=255)

                                                                                                                            tGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGAGaa              >  1:1262708/1‑140 (MQ=255)

                                                                                                                            tGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACaa              >  1:2279198/1‑140 (MQ=255)

                                                                                                                            tGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACaa              >  1:586114/1‑140 (MQ=255)

                                                                                                                             taaaTGAAGAGTCTACTTATTTTCATTTTTTTACGTGCCATTAAAACCTTCCTTAGATTTAAAAGATTCCGAATGTAAAAGAATCTAACTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACaaa             <  1:271864/139‑1 (MQ=255)

                                                                                                                             gAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACaaa             <  1:157304/140‑1 (MQ=255)

                                                                                                                                      agTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGaaa    >  2:28617/1‑140 (MQ=255)

                                                                                                                                        tCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAg  >  2:224054/1‑140 (MQ=255)

                                                                                                                                        tCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAg  >  2:1859455/1‑140 (MQ=255)

ACAGGCTGTCTATAAATACCTGCTGTTCATCAATTTCTTCGGGTTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAG  >  NZ_CP034943/2995423‑2995698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`