Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 438,597 G→A 5.3% A46A (GCC→GCT gabR ← transcriptional regulator GabR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943438,5970GA 5.3% 627.7 / 15.1 189A46A (GCC→GCTgabRtranscriptional regulator GabR
Reads supporting (aligned to +/- strand):  ref base G (71/108);  new base A (4/6);  total (75/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 190 total aligned reads displayed.

ATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATT  >  NZ_CP034943/438458‑438735
                                                                                                                                           |                                                                                                                                          
  ggggggTGCTCCACGGCAGAAAACAGGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACACATAGCCCTCAGCCAGAAGCTGCTGATAGGCGGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGc                                                                                                                                          <  1:1205277/140‑1 (MQ=255)
   gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                         >  1:100111/3‑140 (MQ=255)
   gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                         >  1:1127422/3‑140 (MQ=255)
   gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                         >  1:397235/3‑140 (MQ=255)
   gagggTGCTCCTCGGCGGAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGcc                                                                                                                                         >  1:621177/3‑140 (MQ=255)
     gggTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAg                                                                                                                                       >  2:524815/1‑140 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                     <  1:1805021/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                     <  1:1961474/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                     <  1:1402104/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                     <  1:591277/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                     <  2:925159/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                     <  2:961335/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCATCAGCTTCTCGGCCAGct                                                                                                                                     <  1:2415839/140‑1 (MQ=255)
       gTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAAGGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGct                                                                                                                                     <  1:2283995/140‑1 (MQ=255)
         gCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctct                                                                                                                                   >  2:1070964/1‑140 (MQ=255)
         gCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctct                                                                                                                                   <  1:2015774/140‑1 (MQ=255)
         gCTCCACGGCAGAAAACATGTCCAGCGTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATAGCCCGCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctct                                                                                                                                   <  1:698905/140‑1 (MQ=255)
          cTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctc                                                                                                                                  >  2:266132/1‑140 (MQ=255)
          cTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctc                                                                                                                                  >  1:1008180/1‑140 (MQ=255)
            ccACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctctctc                                                                                                                                <  2:298003/140‑1 (MQ=255)
             ctcggcggAAAACATGTCTAGTTCCTCCACGAAGAAACCCTTTCGTTCAATGGCGTACAAATACCCCTCAGCCAGCAGCTGCTGATAGGCTGAATTCACTGAATTTACGCTGACCTTGAGATTTTCAGCCAGctcccgct                                                                                                                               <  2:1205147/138‑6 (MQ=255)
               cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                             >  1:1269634/1‑140 (MQ=255)
               cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                             >  1:1808203/1‑140 (MQ=255)
               cGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTg                                                                                                                             <  2:891957/140‑1 (MQ=255)
                 gCAGAACAAATGTCCAGCTTCTCCCCGAAAACCCCCTGTCGCTCAATGGCGTACAAATATCCCTCACCCAGAAGCTGCCGCTAGGCTGAATTCACTGAGTTTACGCTGACCTGCAGCTTCTCGGCCAGCTCTCTCTTGGa                                                                                                                           <  2:501739/140‑1 (MQ=255)
                  cAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAg                                                                                                                          >  2:411574/1‑140 (MQ=255)
                            gTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCg                                                                                                                <  2:44127/140‑1 (MQ=255)
                               cAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAgt                                                                                                             >  1:2245631/1‑140 (MQ=255)
                                  cTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGtgtg                                                                                                          >  2:2311140/1‑140 (MQ=255)
                                      tcCACGTAAAACCCATTTCGCTCAATTGCGTTCAAATATCCCTCAGCCAGATGCTGCTGTTAGGCTGAATTCACTGAGTTGACGCTGACCTTCAGCTTCTCGGGCAGCTCTCTCTTGGAGGGGAGCTTCGAGTGTTgcag                                                                                                      <  2:1348496/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTGAAGGGCGGACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGCTTACGCTGACCTTCAGGGTCTCGGCCAGCGCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                      <  2:2014946/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                      <  2:1912651/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                      <  2:1367315/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                      <  2:1336329/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                      <  1:2118609/140‑1 (MQ=255)
                                      tcCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                      <  1:1042564/140‑1 (MQ=255)
                                      gccACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCGGCTGATAGGCTGAAGTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGgcag                                                                                                      <  2:1607764/139‑1 (MQ=255)
                                           gAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCGTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGAt                                                                                                 <  1:1650396/140‑1 (MQ=255)
                                                  cccTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCt                                                                                          >  1:2277529/1‑140 (MQ=255)
                                                       tCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGaa                                                                                     >  1:2081827/1‑140 (MQ=255)
                                                          cTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAAttt                                                                                  >  1:1811270/1‑140 (MQ=255)
                                                               tGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCtttt                                                                             >  1:589286/1‑140 (MQ=255)
                                                                  cGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAgg                                                                                                                         >  1:1466645/1‑93 (MQ=255)
                                                                    tACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTTGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAg                                                                        >  2:1417446/1‑140 (MQ=255)
                                                                    tACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAg                                                                        >  1:1070552/1‑140 (MQ=255)
                                                                         atatCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGttttt                                                                   >  1:1088496/1‑140 (MQ=255)
                                                                         atatCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGttttt                                                                   >  1:763274/1‑140 (MQ=255)
                                                                           atCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGctc                                                                                                                                    <  1:1360008/73‑1 (MQ=255)
                                                                           atCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGa                                                                 >  2:534606/1‑140 (MQ=255)
                                                                            tCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGAt                                                                >  2:951733/1‑140 (MQ=255)
                                                                                 cAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAAtt                                                           >  2:518340/1‑140 (MQ=255)
                                                                                    ccAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGc                                                        >  1:1392414/1‑140 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                     <  2:1683597/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                     <  2:1503665/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                     <  2:1795722/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                     <  2:2203670/140‑1 (MQ=255)
                                                                                       gAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTga                                                     <  1:313707/140‑1 (MQ=255)
                                                                                              ctgATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGatat                                              >  1:1271727/1‑140 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACTCTTACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGTACCTTCGAGTGTGGCAGAAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  2:2029410/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  2:941325/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  2:621444/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  1:2071842/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  2:184262/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  1:888514/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  2:1010415/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  2:2277529/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCGGATAGATATaa                                            <  2:745751/140‑1 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTGTTTGATAAATTTGCTGATAGAtatac                                            <  2:1017572/140‑2 (MQ=255)
                                                                                                gATAGGCTGAATTCACTGAGTTTACGCTGAACTTCATCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTTTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATaa                                            <  1:926920/140‑1 (MQ=255)
                                                                                                    ggCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCgg                                        <  2:147433/140‑1 (MQ=255)
                                                                                                     gCTGACTTCACTGAGTTTACGCGGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  2:1174001/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  1:518340/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  2:2245631/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  1:1547614/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  1:1593134/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  1:2311140/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  2:1717626/140‑1 (MQ=255)
                                                                                                     gCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  1:430594/140‑1 (MQ=255)
                                                                                                     gCTGAATTCAATGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  2:1374339/140‑1 (MQ=255)
                                                                                                               gtGAGTTTACTCTTAGCTTGAGGTTATCGTCCTGTTTTGTTTTGGTGGGGAGCTTCGATTGTGGCAGAATATTACTGCTGTTAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGTTAGTTATATTCGGTTTGTTTTGAg                             <  2:2174474/139‑1 (MQ=255)
                                                                                                               cTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAg                             <  2:850278/140‑1 (MQ=255)
                                                                                                               cTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAg                             <  2:1392414/140‑1 (MQ=255)
                                                                                                                    tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                        <  1:448435/140‑1 (MQ=255)
                                                                                                                    tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                        <  1:884682/140‑1 (MQ=255)
                                                                                                                    tttACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTc                        >  1:1420899/1‑140 (MQ=255)
                                                                                                                       ccGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTGGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg                     <  2:2133966/139‑1 (MQ=255)
                                                                                                                       aCGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAg                     <  2:1292592/140‑1 (MQ=255)
                                                                                                                        cGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGc                                       <  1:1737763/121‑1 (MQ=255)
                                                                                                                           tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta                 <  2:397235/140‑2 (MQ=255)
                                                                                                                           tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta                 <  2:1582267/140‑2 (MQ=255)
                                                                                                                           tGACCTTGAGATTTTCAGCCAGCTCCCGCTTGGAGGGAACCTTCGAGTGCGGCAGCAGATTGCGGCTGAGGATTTCTTTTTTCAGCTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAACGATCGAgtgta                 <  2:100111/140‑2 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa                <  1:259773/140‑1 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa                >  1:2326792/1‑140 (MQ=255)
                                                                                                                            gACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGa                >  2:1832870/1‑140 (MQ=255)
                                                                                                                                    gCTTCTCGGCCAGCTCGCGCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATg        <  2:1454480/140‑1 (MQ=255)
                                                                                                                                       tctcGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTcc     >  2:202061/1‑140 (MQ=255)
                                                                                                                                        ctcGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCa    >  2:2141747/1‑140 (MQ=255)
                                                                                                                                        ctcGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAAGTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCa    >  1:1363448/1‑140 (MQ=255)
                                                                                                                                         tcGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCAt   >  1:1154646/1‑140 (MQ=255)
                                                                                                                                          cGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCAtt  <  1:827976/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
ATGGGGGGTGCTCCACGGCAGAAAACATGTCCAGCTTCTCCACGAAAAACCCCTTTCGCTCAATGGCGTACAAATATCCCTCAGCCAGAAGCTGCTGATAGGCTGAATTCACTGAGTTTACGCTGACCTTCAGCTTCTCGGCCAGCTCTCTCTTGGAGGGGACCTTCGAGTGTGGCAGCAGATTACGGCTGAGAATTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATT  >  NZ_CP034943/438458‑438735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: