Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,995,605 G→A 5.1% intergenic (‑17/+143) lytG ← / ← EO946_RS15510 exo‑glucosaminidase LytG/MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,995,6050GA 5.1% 376.2 / 6.7 119intergenic (‑17/+143)lytG/EO946_RS15510exo‑glucosaminidase LytG/MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (80/31);  new base A (3/3);  total (84/35)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 120 total aligned reads displayed.

TTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGAAAGGTTTTGATCAT  >  NZ_CP034943/2995466‑2995743
                                                                                                                                           |                                                                                                                                          
ttCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAg                                                                                                                                            >  1:630119/1‑140 (MQ=255)
    ttGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTaa                                                                                                                                        >  1:1221552/1‑140 (MQ=255)
            ttCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTAc                                                                                                                                <  1:999994/140‑1 (MQ=255)
            ttCAATCGGTTTTGATAAAGTTGTCGCTAAAACAAACAGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTAc                                                                                                                                >  1:1312095/1‑140 (MQ=255)
            ttCAATCGGTTTTGATAAAGTTGTCGCTAAAACAAACAGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTAc                                                                                                                                >  1:333879/1‑140 (MQ=255)
            ttCAATCGGTTTTGATAAAGTTGTCGCTAAAACAAACAGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTAc                                                                                                                                >  1:330587/1‑140 (MQ=255)
             tCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGACTCGATACAATCAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCGTCGAGTTAAAAGATTAct                                                                                                                               >  2:2401602/1‑139 (MQ=255)
              cTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGa                                                                                                                              <  2:290859/140‑1 (MQ=255)
              cTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGa                                                                                                                              <  2:2340747/140‑1 (MQ=255)
                    gTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaa                                                                                                                        <  1:808080/140‑1 (MQ=255)
                    gTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaa                                                                                                                        <  1:1881441/140‑1 (MQ=255)
                     ttttTATAATGTTGTGTCTTAAACAAACAGGGCTAGCGTAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCATTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaaa                                                                                                                       <  2:1509758/140‑1 (MQ=255)
                      tttGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaaaa                                                                                                                      <  1:1124818/140‑1 (MQ=255)
                      tttGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaaaa                                                                                                                      <  1:1696311/140‑1 (MQ=255)
                      tttGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTaaaa                                                                                                                      <  1:1131141/140‑1 (MQ=255)
                                  gtgGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCttt                                                                                                          <  1:935712/140‑1 (MQ=255)
                                   tgGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCtttt                                                                                                         >  1:2145368/1‑140 (MQ=255)
                                   tgGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAAAAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTAGAGTAAAAAGATTACGAATGTAAAAGAATCTAACtttt                                                                                                         >  1:1659318/1‑140 (MQ=255)
                                    ggCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAATGATTACGTATGTAAAAGTATCTACCTTTTa                                                                                                        >  2:1815371/1‑140 (MQ=255)
                                        aaaaCAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACATTTTAGAAt                                                                                                    >  1:445874/1‑140 (MQ=255)
                                          aacaaacaGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCATTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAAttt                                                                                                  >  2:1594296/1‑140 (MQ=255)
                                          aacaaacaGGGCTAGCGGAATCGATACAATCAAAAATAGTGAAATGAAGAGTCTACGTTTGTTCAGTTTTTTACGGGCCATGAAAACCTTCGTTCGAGTTAAAAGATTACGAATGTAAAAGAATCGACCTGTTAGTAttt                                                                                                  >  2:1736433/1‑140 (MQ=255)
                                               acaGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACtt                                                                                             >  2:1553499/1‑140 (MQ=255)
                                                caGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                                                                            <  2:413647/140‑1 (MQ=255)
                                                caGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                                                                            <  2:1612013/140‑1 (MQ=255)
                                                caGGGCTAGAGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                                                                            <  2:244131/140‑1 (MQ=255)
                                                caGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                                                                            <  2:1312095/140‑1 (MQ=255)
                                                caGAGCTAGCGGAATCGATACAAGGAAAAATAGTGAAATCAAGAGTTTACGTTTTTTAAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAATTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTg                                                                                            <  2:333879/140‑1 (MQ=255)
                                                   ggCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCAc                                                                                         >  2:392679/1‑140 (MQ=255)
                                                   ggCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACATTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCAc                                                                                         >  2:2222523/1‑140 (MQ=255)
                                                     cTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAATAATCTACCTTTTAGAATTTTAATTGCACtt                                                                                       >  2:1331383/1‑140 (MQ=255)
                                                     cTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACtt                                                                                       >  2:89686/1‑140 (MQ=255)
                                                     cTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACtt                                                                                       >  2:1094582/1‑140 (MQ=255)
                                                        gCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTa                                                                                    >  1:147769/1‑140 (MQ=255)
                                                             aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAtt                                                                               >  1:2092966/1‑140 (MQ=255)
                                                             aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAtt                                                                               >  1:1969656/1‑140 (MQ=255)
                                                             aTCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAtt                                                                               >  2:1522724/1‑140 (MQ=255)
                                                              tCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAttt                                                                              >  1:1868365/1‑140 (MQ=255)
                                                              tCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAttt                                                                              >  1:1832007/1‑140 (MQ=255)
                                                              tCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATAttt                                                                              >  1:1832000/1‑140 (MQ=255)
                                                                  tACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCCGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAACAGAATCTCCCTTTTAGAATTTTACTTGCACTTCTACTATTTTTCa                                                                          >  2:1993725/1‑140 (MQ=255)
                                                                  tACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCa                                                                          <  1:1973312/140‑1 (MQ=255)
                                                                     aaTGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTAAAtt                                                                       >  1:1275880/1‑140 (MQ=255)
                                                                         aaaaaTAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCttt                                                                   <  2:991096/140‑1 (MQ=255)
                                                                         aaaaaTAGTGAAATGAAGACTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCttt                                                                   <  2:1976603/140‑1 (MQ=255)
                                                                          aaaaTAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTAAATTCTTTa                                                                  >  2:1762346/1‑140 (MQ=255)
                                                                                gTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCGTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACa                                                            <  2:2000162/140‑1 (MQ=255)
                                                                                 tGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGAGaa                                                           >  1:1262708/1‑140 (MQ=255)
                                                                                 tGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACaa                                                           >  1:2279198/1‑140 (MQ=255)
                                                                                 tGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACaa                                                           >  1:2294002/1‑140 (MQ=255)
                                                                                 tGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACaa                                                           >  1:586114/1‑140 (MQ=255)
                                                                                  gAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACaaa                                                          <  1:157304/140‑1 (MQ=255)
                                                                                           agTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGaaa                                                 >  2:2015423/1‑140 (MQ=255)
                                                                                           agTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGaaa                                                 >  2:28617/1‑140 (MQ=255)
                                                                                             tCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAg                                               >  2:1859455/1‑140 (MQ=255)
                                                                                             tCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAg                                               >  2:224054/1‑140 (MQ=255)
                                                                                               tACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGAt                                             >  1:1427006/1‑140 (MQ=255)
                                                                                               tACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGAt                                             >  1:885752/1‑140 (MQ=255)
                                                                                               tACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGAt                                             >  1:1295813/1‑140 (MQ=255)
                                                                                               tACGTTTTTTCAGTTTTTTAAGGGCCATGAAAACCTTACTTCGATTTAAAAGATTACGAATGTAAAAGAATCTAACTTTTAGAATTTTACATGGACTTCTAATATTTTTAAATTCTTTAGGGACAAAGTTGGGAAAAGAt                                             >  2:1608642/1‑140 (MQ=255)
                                                                                                 cGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGGACTTCTAATATTTTTCAATTCTTTAGGGAGAAAGTTGGGATAAGATTg                                           >  2:1508484/1‑140 (MQ=255)
                                                                                                  gTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGAtttc                                          >  1:59200/1‑138 (MQ=255)
                                                                                                  gTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGc                                          >  2:1521399/1‑140 (MQ=255)
                                                                                                  gTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGc                                          >  2:1519715/1‑140 (MQ=255)
                                                                                                  gTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGc                                          >  1:545536/1‑140 (MQ=255)
                                                                                                     ttttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCAGTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaa                                       >  1:40565/1‑140 (MQ=255)
                                                                                                      tttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGACTGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTATTATTTTTCAATTCTTTAGGGACAAAGTTTGTAAAAGATTGcaaac                                      >  2:511860/1‑140 (MQ=255)
                                                                                                      tttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaac                                      >  2:147360/1‑140 (MQ=255)
                                                                                                      tttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaa                                      >  2:96689/1‑139 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGTAAACCTTCCTTCGAGTTAAAAGAATACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTGGTAATATTTTTGAATTCTTTAGGGACAAACTTGGGAAAAGATTTcaaaca                                     >  1:803703/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGccaaca                                     >  1:1380441/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcacaca                                     >  1:1351953/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaca                                     >  1:1329371/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaca                                     >  1:1380506/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaca                                     >  1:1954715/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaca                                     >  1:1707579/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaca                                     >  1:2099414/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaca                                     >  1:2253095/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaca                                     >  2:109470/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaaaca                                     >  1:602134/1‑140 (MQ=255)
                                                                                                       ttCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTGCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGcaatca                                     >  1:4155/1‑140 (MQ=255)
                                                                                                           gTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACaaaaa                                 <  1:1859455/140‑1 (MQ=255)
                                                                                                           gTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACaaaaa                                 <  2:1427006/140‑1 (MQ=255)
                                                                                                                ttACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTAGCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCtt                            <  2:1252632/140‑1 (MQ=255)
                                                                                                                ttACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCTATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCtt                            <  2:1295813/140‑1 (MQ=255)
                                                                                                                ttACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCtt                            <  2:1039490/140‑1 (MQ=255)
                                                                                                                      gCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTATGGACAATGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTc                      >  2:801390/1‑140 (MQ=255)
                                                                                                                      gCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAAATCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTc                      >  2:2240605/1‑140 (MQ=255)
                                                                                                                          tGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAgg                  >  2:2243867/1‑140 (MQ=255)
                                                                                                                          tGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTATTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAgg                  >  2:765443/1‑140 (MQ=255)
                                                                                                                            aaaaCCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGa                <  2:1954715/140‑1 (MQ=255)
                                                                                                                                ccttccttCGAGTTAAAAGATTACGTATGTAAAAGAATCTTCCTTTTAGAATTTTACTTGCCCTTCTAATATTTTTCAATTCTGTCGGGACTAGGGTGGGAAAAGATTGCAAACAAAATACCTTTCCCTCAAGGGAACgg            >  1:1079395/1‑140 (MQ=255)
                                                                                                                                ccttccttCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGACAgg            >  1:1338693/1‑140 (MQ=255)
                                                                                                                                ccttccttCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGAAAgg            <  2:2099414/140‑1 (MQ=255)
                                                                                                                                    ccttcGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGAAAGGtttt        >  1:262710/1‑140 (MQ=255)
                                                                                                                                    ccttcGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGAAAGGtttt        >  2:2176501/1‑140 (MQ=255)
                                                                                                                                     cttcGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTATAATTTTACTTGCACTTCTATTATTTTTCAATTCTTTAGGGACAAAGGTGGGAAATGATTGCCAACAAAAAACCTTTCCCTCAAGGGACAGGTTTTg       >  2:1032058/1‑140 (MQ=255)
                                                                                                                                        cGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGAAAGGTTTTGATc    <  1:1553499/140‑1 (MQ=255)
                                                                                                                                        cGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGAAAGGTTTTGATc    >  2:501310/1‑140 (MQ=255)
                                                                                                                                          aGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGACAGGTTTTgatgat  >  1:778923/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTCTTTGGATGTTTCTATCGGTTTTGATAATGTTGTGGCTAAAACAAACAGGGCTAGCGGAATCGATACAATGAAAAATAGTGAAATGAAGAGTCTACGTTTTTTCAGTTTTTTACGGGCCATGAAAACCTTCCTTCGAGTTAAAAGATTACGAATGTAAAAGAATCTACCTTTTAGAATTTTACTTGCACTTCTAATATTTTTCAATTCTTTAGGGACAAAGTTGGGAAAAGATTGCAAACAAAAAACCTTTCCCTCAAGGGAAAGGTTTTGATCAT  >  NZ_CP034943/2995466‑2995743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: