Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,012,140 G→A 5.3% intergenic (‑108/+21) mcpA ← / ← tlpA methyl‑accepting chemotaxis protein/methyl‑accepting chemotaxis protein TlpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,012,1400GA 5.3% 230.0 / 2.2 75intergenic (‑108/+21)mcpA/tlpAmethyl‑accepting chemotaxis protein/methyl‑accepting chemotaxis protein TlpA
Reads supporting (aligned to +/- strand):  ref base G (35/35);  new base A (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTTGTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCGGCTGAAGCGGCG  >  NZ_CP034943/3012001‑3012278
                                                                                                                                           |                                                                                                                                          
cTTTGTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatag                                                                                                                                            >  1:1582283/1‑140 (MQ=255)
cTTTGTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatag                                                                                                                                            >  1:2276389/1‑140 (MQ=255)
 tttGTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAataga                                                                                                                                           <  2:2336734/140‑1 (MQ=255)
 tttGTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTGATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAataga                                                                                                                                           <  2:517704/140‑1 (MQ=255)
   tGTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatagata                                                                                                                                         >  1:551884/1‑140 (MQ=255)
    gTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCATGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatagatag                                                                                                                                        <  2:1926938/140‑1 (MQ=255)
    gTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatagatag                                                                                                                                        <  2:689337/140‑1 (MQ=255)
    gTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatagatag                                                                                                                                        <  2:1593196/140‑1 (MQ=255)
    gTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatagatag                                                                                                                                        <  2:726527/140‑1 (MQ=255)
    gTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatagatag                                                                                                                                        <  2:933249/140‑1 (MQ=255)
    gTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatagatag                                                                                                                                        <  1:1221098/140‑1 (MQ=255)
    gTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTAACCTTCATATCGTCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAatagatag                                                                                                                                        <  2:2425342/140‑1 (MQ=255)
         ctCAGTTGGAGTATTTTTTTCAGCATTCATTTCACCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTAtt                                                                                                                                   <  1:1316449/139‑1 (MQ=255)
         aTCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTAtt                                                                                                                                   <  2:715802/140‑1 (MQ=255)
             gTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGa                                                                                                                               >  2:2074957/1‑140 (MQ=255)
              ttGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGAc                                                                                                                              <  1:1406788/140‑1 (MQ=255)
              ttGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGAc                                                                                                                              <  1:1613954/140‑1 (MQ=255)
              ttGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGAc                                                                                                                              <  1:334925/140‑1 (MQ=255)
              ttGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGAc                                                                                                                              <  1:1728945/140‑1 (MQ=255)
              ttGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTACATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGAc                                                                                                                              <  1:1042934/140‑1 (MQ=255)
              ttGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCGTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAAGTTCCCATAAATAGATAGTTATTTAGAc                                                                                                                              <  1:432862/140‑1 (MQ=255)
                ggAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCTGGAAATGGGTAAATATATACATCAAAAAAGTAGAAATTTAACAAAAGTAAATAATTTCCCATAAATAAATAGAATTTTAGAcat                                                                                                                            >  1:1577145/1‑138 (MQ=255)
                ggAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCTGGAAATGGGTAAATATATACAGCAAAAAAGAAGAAATTTAACAAAAGTAACTAATTTCCCATAAATAAATAGTATTTTAGACCt                                                                                                                            >  1:1217335/1‑140 (MQ=255)
                      tttttttCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTACCATAAATAGATAGTTATTTAGAACTATGCtt                                                                                                                      >  2:281796/1‑140 (MQ=255)
                          tttCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGTCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATc                                                                                                                  <  2:1942006/140‑1 (MQ=255)
                          tttCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATc                                                                                                                  <  2:905308/140‑1 (MQ=255)
                                      tttCTCCTTTTTTATGCGACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCg                                                                                                      <  2:2412636/140‑1 (MQ=255)
                                         ctcCATTTTTATGCTACCCTTCATATCGGCATGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATACACTTTAAATCGttt                                                                                                   <  1:1693878/140‑1 (MQ=255)
                                         atcCTTTTTTATGCTACCCTTAATATCGGCAGGAAATGGGTAAATATATACAGTGCAAAATAAGAAATTTAACGAAAGTAACTAATTTACCATAAATAGATAGTTATTTATACCTATTCTTAATCCACTTTAAATCGttt                                                                                                   <  1:408183/139‑1 (MQ=255)
                                                tttATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAgg                                                                                            <  1:404193/140‑1 (MQ=255)
                                                tttATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAAGAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAgg                                                                                            <  1:28102/140‑1 (MQ=255)
                                                     gCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACg                                                                                       <  2:1072570/140‑1 (MQ=255)
                                                     gCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACg                                                                                       <  2:979735/140‑1 (MQ=255)
                                                     gCTACCCTACATATCGGCAGGAAATGGGTAAATATAAACACTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACg                                                                                       <  1:160026/140‑1 (MQ=255)
                                                        aCCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAg                                                                                    <  1:1226207/140‑1 (MQ=255)
                                                        aCCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAg                                                                                    <  1:1907306/140‑1 (MQ=255)
                                                                   cGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACa                                                                         >  1:2192761/1‑140 (MQ=255)
                                                                     gCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACAtt                                                                       >  2:1586032/1‑140 (MQ=255)
                                                                       aGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCt                                                                     >  2:469782/1‑140 (MQ=255)
                                                                       aGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCt                                                                     >  2:131524/1‑140 (MQ=255)
                                                                       aGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCt                                                                     >  2:1007843/1‑140 (MQ=255)
                                                                                  aaaaATATACAGATAAAAAGAACAAATTTAACGAAACTAACTAATTTCCCATAAATAGATAATTATTTAGACCTATTCTTAATCCACTTTAAATGCTTTTGTCAGGACACGGAGTTCTTCAGACATTCTTTAAATCGTca                                                          >  2:399236/1‑139 (MQ=255)
                                                                                        ataCACTTAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATCGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCg                                                    <  2:1172343/140‑1 (MQ=255)
                                                                                         taCAGCAAAAAAGAAGAAATTTAACAAAAGTAACTAATTTCCCATAAATAAATAGTATTTTAGACCTATTATTTGTCTACTTTAAATTGTTTTGTCAGATCACGGAGTTCTTCAGACATTCTTTCAAGCGTCAGTGCAGa                                                   <  2:1217335/140‑1 (MQ=255)
                                                                                         taCAGAAAAAAATAAGAAATTTAACAAAAGTAACTAATTTCCCATAAATAAATAGTATTTTAGACCTATTATTTGTCTACTTTAAATTGTTTTGTCAGATCACGGAGTTCTTCAGACATTCTTTCAAGCGTCAGTGCAGa                                                   <  2:1577145/140‑1 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATATATAGTTCGTTAGACGTCGTCTTAATCCACTGTAAATCGTTGTGTGAGGTCACGGAGTTCGGCTGTCATTCTTTCTAGCGTGTGTGCCGTGGAAg                                              >  1:1422777/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:2391531/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:2032099/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  2:1650964/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  2:166368/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:1946358/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:1910093/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  2:2238947/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  2:2244211/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:1490853/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  2:438869/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:2360656/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:2136997/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:2058172/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:773766/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAg                                              >  1:2392122/1‑140 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGGTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGacg                                              >  2:2142210/1‑138 (MQ=255)
                                                                                              tGAAAAAGAAGAAATTTAACGAAAGTAAATAATTTCCAATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCAAGTAGTTATTCAGAAATTATTTCTAGATTCTGTGCCGATTAAg                                              >  1:1357092/1‑140 (MQ=255)
                                                                                               gAAAAAGAAGAAATTTAAGGAAAGTAACTAATTTCCGATAAATAGGTAGTTCTTTCTTCCTCTGGTTAATCGTCTTTAACTCTTTTTGTGAGGTCTCGGAGTGCTTCCGACCTTCTTTGGAGCGTGTGTGCGGATgtcgc                                             >  2:1377892/1‑136 (MQ=255)
                                                                                               gAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGc                                             >  2:552655/1‑140 (MQ=255)
                                                                                                aaaaaGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCg                                            >  2:1702161/1‑140 (MQ=255)
                                                                                                aaaaaGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCg                                            >  2:1591172/1‑140 (MQ=255)
                                                                                                aaaaaGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCg                                            >  2:672641/1‑140 (MQ=255)
                                                                                                aaaaaGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCg                                            >  1:244371/1‑140 (MQ=255)
                                                                                                  aaaGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGAt                                          <  2:667498/140‑1 (MQ=255)
                                                                                                  aaaGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAAGAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGAt                                          <  1:1943927/140‑1 (MQ=255)
                                                                                                   aagaagAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGAtt                                         <  2:2391531/140‑1 (MQ=255)
                                                                                                      aagaaATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTtcc                                      <  1:2140221/140‑1 (MQ=255)
                                                                                                      aagaaATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTtcc                                      <  1:2417946/140‑1 (MQ=255)
                                                                                                      aagaaATATAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACATTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTtcc                                      <  1:556069/140‑1 (MQ=255)
                                                                                                          aaTTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTATTGTCAGGTCACGGAGTTCTTCAGACAGTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCa                                  <  2:1998130/140‑1 (MQ=255)
                                                                                                            tttAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATg                                <  1:2244211/140‑1 (MQ=255)
                                                                                                               aaCGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGAt                             <  2:1283160/140‑1 (MQ=255)
                                                                                                                     aGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGc                       <  1:1434206/140‑1 (MQ=255)
                                                                                                                     aGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGc                       <  1:2368540/140‑1 (MQ=255)
                                                                                                                     aGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGc                       >  1:1868737/1‑140 (MQ=255)
                                                                                                                             aTTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGAt                                          <  1:1874050/113‑1 (MQ=255)
                                                                                                                             aTTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGAt                                          >  2:1874050/1‑113 (MQ=255)
                                                                                                                               ttCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCgg             <  1:1702161/140‑1 (MQ=255)
                                                                                                                               ttCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCgg             <  1:1591172/140‑1 (MQ=255)
                                                                                                                                tCCCATAAATAGATCGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCGGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCGGc            >  1:351722/1‑140 (MQ=255)
                                                                                                                                   cATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCGGCTGa         >  2:1695373/1‑140 (MQ=255)
                                                                                                                                        atagatagTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCGGCTGAAgcgg    <  2:1868737/140‑1 (MQ=255)
                                                                                                                                          agatagTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCGGCTGAAgcggcg  <  2:712367/140‑1 (MQ=255)
                                                                                                                                          agatagTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCGGCTGAAgcggcg  <  1:2166830/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
CTTTGTTTTATCAGTTGGAGTATTTTTTTCATCATTCATTTCTCCTTTTTTATGCTACCCTTCATATCGGCAGGAAATGGGTAAATATATACAGTGAAAAAGAAGAAATTTAACGAAAGTAACTAATTTCCCATAAATAGATAGTTATTTAGACCTATTCTTAATCCACTTTAAATCGTTTTGTCAGGTCACGGAGTTCTTCAGACATTCTTTCAAGCGTCTGTGCCGATGAAGCGATTTCCTCCATGGATGCCAGCTGTTCCTCGGCTGAAGCGGCG  >  NZ_CP034943/3012001‑3012278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: