Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 3,036,047 T→C 8.0% H10H (CAT→CAC kinB → sporulation sensor histidine kinase KinB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,036,0470TC 8.0% 223.4 / 8.4 75H10H (CAT→CACkinBsporulation sensor histidine kinase KinB
Reads supporting (aligned to +/- strand):  ref base T (41/28);  new base C (3/3);  total (44/31)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

GCCTTTACGTCATTCGTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCC  >  NZ_CP034943/3035910‑3036184
                                                                                                                                         |                                                                                                                                         
gCCTTTACGTCATTCGTAAAGGCGTTATTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCatat                                                                                                                                         <  2:1214919/140‑1 (MQ=255)
    ttACGTCATTCGTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGc                                                                                                                                     >  2:624820/1‑140 (MQ=255)
     tACGTCATTCGTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAATGATTTCACACGAATGGAAATTCTAAAAGTCTATCTCCTGCATATCTGCt                                                                                                                                    >  2:1471002/1‑140 (MQ=255)
     tACGTCATTCGTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCt                                                                                                                                    <  2:562663/140‑1 (MQ=255)
     tACGTCATTCGTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCt                                                                                                                                    >  2:1764723/1‑140 (MQ=255)
            ttCGTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTAtttt                                                                                                                             >  2:993964/1‑140 (MQ=255)
            ttCGTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTAtttt                                                                                                                             >  2:2187750/1‑140 (MQ=255)
               gTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGtt                                                                                                                          <  1:1822395/140‑1 (MQ=255)
               gTAAAGGCGTTATTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGtt                                                                                                                          <  2:158219/140‑1 (MQ=255)
                 aaaGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTc                                                                                                                        >  1:294978/1‑140 (MQ=255)
                  aaGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTcc                                                                                                                       >  1:1085166/1‑140 (MQ=255)
                    ggCGTTCTTTATACAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTa                                                                                                                     <  1:386598/140‑1 (MQ=255)
                                             atTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCt                                                                                            <  1:1240946/140‑1 (MQ=255)
                                             atTTTACTTATAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCt                                                                                            <  1:976867/140‑1 (MQ=255)
                                                 tACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGa                                                                                        >  1:196590/1‑140 (MQ=255)
                                                 tACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGa                                                                                        >  1:55064/1‑140 (MQ=255)
                                                              tAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGAGTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGATTGGAAATCCGGCGATcc                                                                           >  2:2120247/1‑140 (MQ=255)
                                                                 tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTCTCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTtg                                                                        >  1:591195/1‑140 (MQ=255)
                                                                 tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                        >  1:668707/1‑140 (MQ=255)
                                                                 tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                        >  1:2374105/1‑140 (MQ=255)
                                                                 tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTTCTGCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTtg                                                                        >  1:2374122/1‑140 (MQ=255)
                                                                 tgtTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTtg                                                                        >  1:722852/1‑140 (MQ=255)
                                                                    tataATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGc                                                                     <  2:1085166/140‑1 (MQ=255)
                                                                     ataataGATTCTTATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                    >  1:598067/1‑140 (MQ=255)
                                                                     ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATGTGCTTTATTTTGTTTCCTCTTCTTCTCTACCAAGTGTTTTGGCTTGGATAGCCGGCGAGCCTTGTGcc                                                                    >  1:1544879/1‑140 (MQ=255)
                                                                     ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                    >  2:1716852/1‑140 (MQ=255)
                                                                     ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                    >  2:1500208/1‑140 (MQ=255)
                                                                     ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                    >  1:1737361/1‑140 (MQ=255)
                                                                     ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                    >  1:1857438/1‑140 (MQ=255)
                                                                     ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                    >  1:1256217/1‑140 (MQ=255)
                                                                     ataataGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGcc                                                                    >  1:2147085/1‑140 (MQ=255)
                                                                           gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                              >  1:369536/1‑140 (MQ=255)
                                                                           gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                              >  1:479822/1‑140 (MQ=255)
                                                                           gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                              >  1:2399023/1‑140 (MQ=255)
                                                                           gATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCGATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAAt                                                              >  1:389148/1‑140 (MQ=255)
                                                                             ttCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAATCCGGCGATCCTTGTGCCCAAAATaa                                                            >  2:2008130/1‑140 (MQ=255)
                                                                             ttCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATaa                                                            >  2:588294/1‑140 (MQ=255)
                                                                                 tatTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAg                                                        >  2:569954/1‑140 (MQ=255)
                                                                                 tatTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAg                                                        >  2:1140911/1‑140 (MQ=255)
                                                                                   ttttATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCg                                                      <  2:2147085/140‑1 (MQ=255)
                                                                                      tcttcGACAGCATTTCACAAGAATCGCAATTCAACAAGACTATCTCCTGCATATCTGATTTATTGTGTTTCCTATTCTGCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCGAAAATAAACAGCGGGt                                                   <  1:666624/136‑1 (MQ=255)
                                                                                      tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                   >  1:1960640/1‑140 (MQ=255)
                                                                                      tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                   >  1:1505622/1‑140 (MQ=255)
                                                                                      tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                   >  1:2044832/1‑140 (MQ=255)
                                                                                      tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                   >  1:834876/1‑140 (MQ=255)
                                                                                      tataCGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGt                                                   >  2:438402/1‑140 (MQ=255)
                                                                                          cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTCCCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                               <  1:733801/140‑1 (MQ=255)
                                                                                          cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                               <  1:2139558/140‑1 (MQ=255)
                                                                                          cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                               <  2:2399023/140‑1 (MQ=255)
                                                                                          cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                               <  1:2370502/140‑1 (MQ=255)
                                                                                          cGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGt                                               <  2:479822/140‑1 (MQ=255)
                                                                                             aaGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCAc                                            >  1:2022106/1‑140 (MQ=255)
                                                                                             aaGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCAc                                            >  1:745009/1‑140 (MQ=255)
                                                                                                gATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTATACCAAGGGTTTTGGCTTGGAAAGCCGGCGCTCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGtt                                         >  2:1244072/1‑140 (MQ=255)
                                                                                                          gAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTgc                               >  2:2106584/1‑140 (MQ=255)
                                                                                                            aTGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTgcgg                             <  1:914612/140‑1 (MQ=255)
                                                                                                            aTGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTgcgg                             <  1:914532/140‑1 (MQ=255)
                                                                                                                 aaTTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCt                        <  1:2187750/140‑1 (MQ=255)
                                                                                                                        aaaGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGtttt                 <  2:196590/140‑1 (MQ=255)
                                                                                                                          aGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTAt               <  2:722852/140‑1 (MQ=255)
                                                                                                                           gACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATg              <  2:1162111/140‑1 (MQ=255)
                                                                                                                            aCTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGc             <  2:1574794/140‑1 (MQ=255)
                                                                                                                             cTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCa            <  1:1102583/140‑1 (MQ=255)
                                                                                                                             cTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCa            <  1:494501/140‑1 (MQ=255)
                                                                                                                                tctcCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCgg                                                     >  1:1001412/1‑96 (MQ=255)
                                                                                                                                tctcCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCgg                                                     <  2:1001412/96‑1 (MQ=255)
                                                                                                                                    cTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATAtt     <  2:986634/140‑1 (MQ=255)
                                                                                                                                     tGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTc    >  1:550922/1‑140 (MQ=255)
                                                                                                                                      gCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTcc   >  1:2039331/1‑140 (MQ=255)
                                                                                                                                      gCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTATTTTcc   <  2:668707/140‑1 (MQ=255)
                                                                                                                                      gCACATCTGCTTTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTATTTTcc   <  2:2374105/140‑1 (MQ=255)
                                                                                                                                      gCACATCTGCTCTATTTTGTTTCCTATTCTTCTTTACCAAGTGTTTTGGCTTGGAAAACCTGCGATTCTTGTGCCAAAAATAAACAGCGGTTTGGTCACGCTCTTCGCCTGCGGCGCCTCCGTTTTATGCATTATTTTcc   <  2:2374122/140‑1 (MQ=255)
                                                                                                                                       cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCCAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc  <  2:66822/140‑1 (MQ=255)
                                                                                                                                       cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc  >  2:385100/1‑140 (MQ=255)
                                                                                                                                       cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc  <  2:64825/140‑1 (MQ=255)
                                                                                                                                       cATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTccc  <  2:66853/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
GCCTTTACGTCATTCGTAAAGGCGTTCTTTATAAAGGAATTTTATATTTTACTTCTAATATTTAGTGTTATAATAGATTCATATTTTATACGAAAGGATTTCACACGAATGGAAATTCTAAAAGACTATCTCCTGCATATCTGCTTTATTTTGTTTCCTATTCTTCTCTACCAAGTGTTTTGGCTTGGAAAGCCGGCGATCCTTGTGCCCAAAATAAACAGCGGGTTGGTCACGTTATTCGCTTGCGGCGCCTCCGTTTTATGCATTATATTCCC  >  NZ_CP034943/3035910‑3036184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: